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PubMedDate not supplied

[Arbovirus infections in Tunisia: new serological survey of small wild mammals].

Sera of 103 small wild mammals (rodents, insectivora, cheiroptera) trapped at the beginning of 1980 in different areas of Tunisia were studied for antibody against 15 arboviruses. In inhibition haemagglutination tests, positive reactions were found against Dengue type 2 (2.2%), Tahyna (18.4%), Arumowot (42.7%) and Sicilian sandfly fever (31%) viruses. No antibody was found for 8 tick-borne viruses. In comparison with previous sero-surveys, our results indicated a fall of West Nile virus activity and the emergence of Tahyna virus. The possibility that wild cycles involving small mammals and the two phleboviruses may exist in Tunisia is discussed.

Animal Population Groups↗
PubMed1982

[Arbovirus infections in Morocco: serosurvey in small wild mammals in the northern part of the country].

Sera of 128 small wild mammals (rodents, insectivora) trapped in Northern Morocco in March 1979, were studied for antibody against 15 arboviruses. In inhibition-haemagglutination tests, positive reactions were found against West Nile (0.8%), Tahyna (21 %), Arumowot (14 %) and Sicilian Sandfly fever (9.4 %) viruses. In complement fixation tests, an unique serum from Apodemus sylvaticus was simultaneously positive for Quaranfil virus and for a Kemerovo group virus, Brest/Ar/T222, previously isolated from Ornithodoros (A.) maritimus ticks caught in Morocco. These results are discussed according to the bioclimatic profile of the station where the mammals originated and to the potential vectors known to occur here.

Animal Population Groups↗
PubMedDate not supplied

[Factors determining the antigenic activity of tissue cultures from arbovirus-infected diagnostic specimens].

Formation of arbovirus group A and B antigens is influenced significantly by the number of cells, time of monolayer growth before inoculation, the time of maximum accumulation of hemagglutinins, the infectious dose, the growth and maintenance medium composition, the autointerfering activity of the viruses and their sensitivity to tissue inhibitors. The stability of the hemagglutinating and complement-fixing properties of arbovirus diagnostic preparations does not depend upon the degree of concentration (7-12-fold) and the amount of the stabilizer (2-4%), and storage of group A diagnostic preparations in ampoules under vacuum or in ampoules sealed with air for 2-3 1/2 years (the observation period) does not influence their antigenic activity.

Arboviruses↗
PubMed2000

[Serological survey on arbovirus infection in residents of an ecological reserve].

OBJECTIVE: Serological inquires conducted in the Ribeira Valley, S. Paulo State, Brazil, showed an intense circulation of pathogenic arboviruses in the region. The goal was to verify the prevalence of arboviral infections in people living at the local ecological, and its potential association with these population' individual and familiar characteristics. METHODS: The study was carried out among 182 persons of 58 families to identify the presence of antibodies to the following viruses: Rocio (ROC), Ilheus (ILH), Eastern equine encephalitis virus (EEE), Western equine encephalitis virus (WEE), Venezuelan equine encephalitis virus (VEE) and St. Louis encephalitis virus (SLE). Inhibition hemagglutination test and plaque reduction neutralization test were the laboratory assays of choice. MAC-ELISA was used to identify IgM antibodies to ROC, ILH and EEE viruses. RESULTS: The prevalence of antibodies was 26.9% (21. 4% to alphavirus and 12.6% to flavivirus). There were no antibodies to WEE virus. IgM antibodies were not observed suggesting no recent infection in study population. Among the characteristics investigated, age, occupation, place of birth and the habit of going into the forest were shown to be statistically associated with arboviral infection (p<0.05). CONCLUSIONS: It was observed an intense circulation of pathogenic arboviruses, especially VEE. It seems the habit of going into the forest is the most important factor to this population exposure to the mosquito vectors of arboviruses. The results suggest the need of further investigation to clarify the role of birds as arbovirus infection amplificators indoors.

Adolescent↗