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[Content of fluoride, calcium and magnesium in superficial layer of enamel from teeth with vital and necrotic pulp].

This work was designed to determine the content of fluorides, calcium, and magnesium in the superficial layer of enamel of adult teeth. The study group consisted of 25 patients, aged 18 to 35 years, appearing for dental treatment. Two microsamples of the superficial layer of enamel were collected from each patient with the acid biopsy technique. Samples were obtained from pulpectomized teeth treated endodontically and from teeth with necrotic pulp qualifying for endodontic treatment. Control samples were obtained from homonymous, caries-free teeth with vital pulp. The results of biochemical tests in the study group were compared with control teeth.

Adult↗

[The remineralization of enamel lesions by casein phosphopeptide-amorphous calcium fluoride phosphate in vitro].

OBJECTIVE: To evaluate the remineralizing effect on artificial enamel subsurface lesions in vitro by 2% casein phosphopeptide-amorphous calcium fluoride phosphate complexes (CPP-ACFP) solution. METHODS: Twenty-eight enamel blocks were cut from human third molars, in which 2 lesions were prepared from each block. The 28 samples were randomly assigned to the four study groups, in which each group was incubated in remineralization solution for 1, 3, 5, 10 days respectively. After remineralization the samples were sectioned, microradiographied and the mineral content were determined by microdensitometry. RESULTS: The remineralizing solution replaced 9.19%, 14.27%, 29.07%, 38.45% of mineral lost after 1, 3, 5, 10 days respectively (y = 8.9316 x0.6347, R2 = 0.9322; y: remineralizing rate, x: time). Significant differences were found in remineralizing rate between any two groups using One-way Scheffe multiple comparison. CONCLUSIONS: The 2% CPP-ACFP solution could significantly remineralize the artificial enamel subsurface lesion in vitro. The remineralizing rates were increased with the experiment time (within 10 days).

Caseins↗

Relief of osteoporotic backache with fluoride, calcium, and calciferol.

In a prospective randomized clinical trial comprising 22 postmenopausal women with backache and a halisteretic spine with crush fracture(s), 12 women completed a 12-week therapy with sodium fluoride, calcium and calciferol and 10 with placebo. A statistically significant improvement (p less than 0.05), evaluated by a four-stage scale on pains, infirmity, and consumption of analgesics, was observed in the actively treated patients.

Aged↗

Effect on dental caries of a stannous fluoride-calcium pyrophosphate dentrifice in an adult population: one-year results.

The first year of a two-year double-blind clinical trial of the anticaries effectiveness of a stannous fluoride-calcium pyrophosphate (SnF2-CPP) dentifrice as compared to a placebo control dentifrice involved 1,337 adult subjects, 18 to 78 years of age. Adult subjects were examined for dental caries at a baseline examination and after one year of ad lib dentifrice usage by an experienced clinical examiner using a visual-tactile examination and a radiographic evaluation. Results of the study after one year indicate a significant (P < 0.05) 33.3% difference in new caries formation with use of the SnF2-CPP dentifrice as compared to the placebo control based on the DMFS score.

Adolescent↗

Three-year performance of a calcium-, fluoride-, and hydroxyl-ions-releasing resin composite.

Secondary caries is the main reason for replacement of restorations in cross-sectional studies. To prevent demineralization, the use of ion-releasing materials has been suggested. The purpose of this study was to evaluate the durability of a new resin composite that releases calcium-, fluoride- and hydroxyl ions at low pH. Sixty-nine posterior restorations were placed in 36 patients and were evaluated at baseline, 6 months, 1, 2, and 3 years with slightly modified USPHS criteria. Postoperative sensitivity was observed in 2 teeth directly after placement and in another 8 teeth after 6-12 months. Absence of the use of an adhesive bonding technique explained the symptoms. A total of 26% failures was observed during the follow-up: 13 cusp fractures, 2 partial fractures of the resin composite, 1 secondary caries and 1 endodontic treatment due to prolonged sensitivity. The cusp fractures occurred during the second part of the follow-up and may be explained by expansion of the composite material due to water expansion and/or hydrolytic degradation of the alkaline glass filler. It can be concluded that the new ion-releasing resin composite showed, despite promising pH stabilizing properties, a clinically unacceptable failure rate.

Adult↗

Fluoride, calcium and magnesium intake in children living in a high-fluoride area in Ethiopia. Intake through food.

OBJECTIVE: The aim of this study was to assess the daily dietary fluoride intake in children living in two neighbouring villages in the Wonji Shoa Sugar Estate, a rural part of the Ethiopian Rift Valley. METHODS: The villages depended on water from different sources: Village A used either river water (Awash River, with 1.8 mg F(-)/L) or ground water (2.1 mg F(-)/L), while village K was served ground water with 14 mg F(-)/L. Fifteen fully weaned children below the age of 5 years were selected in each of the villages. Over a four-day period the total food intake for these children was assessed by using a duplicate portion technique. The food was analysed for fluoride by using a fluoride ion-selective electrode after the dry ashing. Furthermore, the energy of the food was measured, as well as the calcium and magnesium contents (atomic absorption spectrometry after microwave digestion with nitric acid and hydrogen peroxide). The mothers gave a description of the ingredients used for preparing the food. Relevant background information concerning food habits, etc. was collected through a food frequency questionnaire. RESULTS: This study shows that considerable amounts of fluoride may be retained in food prepared on high-fluoride water. In village A, food contributed 2.3 mg F(-)/day, while a dietary fluoride intake of 4.8 mg/day was found in children in village K. Interestingly, the sevenfold higher fluoride concentration of the water used for food preparation in village K compared to village A, gave only a doubling in fluoride intake through food. Calcium intake was relatively low while magnesium intake was above the recommended level. CONCLUSION: As the energy intake by both groups was low, some systematic underreporting might be suspected. Thus, the fluoride intake in the group may be even higher than what was actually found in this study.

Calcium, Dietary↗

Osteoporosis: calcium, fluoride, and aluminum interactions.

A gradual loss of bone occurs with age. There are indications in the literature that not only calcium but also fluoride may play an important role in maintaining the bone mass. In view of the high incidence of osteoporosis and its complications, and in view of the fact that fluoride is beneficial for the treatment of osteoporosis, studies have been carried out in this research unit to determine the retention and excretion of fluoride both during a normal dietary intake of fluoride and during fluoride supplementation. Another problem to be examined was to delineate whether certain substances interfere with the intestinal absorption of fluoride and of calcium. One such substance is aluminum contained in commonly used antacids. The use of small amounts of aluminum-containing antacids increased fecal fluoride significantly, thereby decreasing the intestinal absorption of fluoride. In addition, the small doses of aluminum-containing antacids also affected the metabolism of calcium and phosphorus, the primary effect being complexation of phosphorus in the intestine leading to phosphorus depletion. This change in phosphorus metabolism was associated with an increase of the urinary and fecal calcium excretion resulting in a negative calcium balance. The dual effect of aluminum, namely in causing calcium loss and inhibition of the intestinal absorption of fluoride, can result in adverse effects on bone which may contribute to bone loss.

Aluminum↗

Fluoride in serum and bone during treatment of osteoporosis with sodium fluoride, calcium and vitamin D.

Thirteen patients with primary osteoporosis were treated for two years with sodium fluoride (NaF), calcium and dihydrotachysterol (group A). The dose of NaF was modified according to the serum fluoride concentration, which was kept as constant as possible between 0.20 and 0.25 microgram/ml. In patients with bone fluoride content greater than or equal to 0.20% a significant increase in the volumetric density and in the surface percentage covered with osteoid and with osteoblasts was observed. A second group of 7 patients with primary osteoporosis was also treated for 2 years with calcium and dihydrotachysterol, but without fluoride (group B). In these patients no significant change in the volumetric density or in the surface percentage covered with osteoid or osteoblasts was found. Patients in group A with a bone fluoride content less than 0.20% could not be distinguished from the patients in group B. Based on the available data an advice was given for the dosage of NaF in patients with osteoporosis. Regular assessment of the serum fluoride concentration remains advisable.

Adult↗

Intraoral evaluation of mineralization of cosmetic defects by a toothpaste containing calcium, fluoride, and sodium bicarbonate.

New dual-phase fluoride toothpastes that contain soluble calcium, phosphate, and baking soda have recently been introduced into the market. These toothpastes are designed to fill in small surface defects in tooth enamel and thereby enhance tooth esthetics such as gloss. This two-part study was designed to assess these superficial mineralizing effects from using one of these products compared with an experimental calcium-containing, bicarbonate-free formulation and a conventional fluoride toothpaste using an intraoral model. Enamel specimens with 4 types of defects were mounted into an intraoral appliance and placed in the mouths of volunteers for 1 month. The four types of defects were whitening toothpaste abrasion, coarse abrasion, natural dimpling, and acid etching. Before and after intraoral exposure, scanning electron microscope photographs of the specimens were made. The surface microhardness of the acid-etched specimens also was determined. The volunteers brushed their specimens twice daily with one of three randomly assigned toothpastes. The toothpastes were a two-phase, calcium-containing, bicarbonate-based toothpaste; an experimental, two-phase, calcium-containing, bicarbonate-free toothpaste; and a conventional toothpaste. Only the calcium-containing toothpastes showed unequivocal signs of mineral deposition into surface defects, leading to smoothing of the enamel. All three products significantly increased the hardness of the etched enamel, presumably because of fluoride. However, only the two calcium-containing toothpastes gave significantly greater hardness increases than the conventional toothpaste; the specimens treated with a conventional toothpaste were indistinguishable from those treated with saliva.

Calcium↗