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At least 73 records · Page 4Linked to original sources
PubMed1996

[Experimental study on repair of mandibular defect by allogenic decalcified bone compounded with autogeneic bone marrow in rabbits].

Twenty adult rabbits were used in our experiment. The bone defects were made at the lower border of the bilateral mandible. A compound bone graft (allogenic decalcified bone with autogeneic bone marrow, ADB) was implanted in the right bone defect as the experimental group. The left mandible was taken as the control group and autogeneic cancellous bone was grafted. The process of new bone formation was evaluated by X-ray plain film, 99Tc-PYP Scintigraphy, histological, and histochemical, and transmmission electronic microscope examinations. The results demonstrated histogically that ADB induced new bone formation. The quantity of new bone produced by compound bone graft in the experimental group was much more than that in control group (P < 0.05). The compound bone graft can promote and accelerate bone defect healing.

Animals↗
PubMed1975

Accelerated reattachment with cementogenesis to dentin, demineralized in situ. I. Optimum range.

This study confirms an original report describing accelerated reattachment with cementogenesis to root dentin, surgically exposed and demineralized in situ. It additionally describes results of 250 experiments on over 1000 teeth in mongrel dogs and cats designed to identify an optimum range of demineralization related to type of agent, pH, and time of application. This optimum range consistently induces flap reattachment with cementogenesis, while demineralization rates above and below this range enhance reattachment relative to undermineralized controls, but do not consistently induce new cementum. Although most controls demonstrated some reattachment with partial cementogenesis, none produced complete repair as did properly demineralized root surfaces and approximately one-third showed spithelial migration to the apical borders of the wound. No demineralized teeth demonstrated this control result. Small species differences in the response to root demineralization and the degree of hypermineralization of roots adjacent to chronic periodontal pockets, may make optimum ranges determined in this animal study slightly low for human pocket reaattachment techniques.

Animals↗
PubMed1995

Comparisons of in vitro root caries models.

The purpose of this article is to compare various model systems for the production of in vitro root caries and to assess their ability to simulate the naturally occurring root caries process. Partially saturated buffer models and gel models were evaluated using polarized light microscopy and both qualitative and quantitative microradiography. All model systems showed very similar lesion formation when examined under polarized light. When microradiographs were compared, the systems which contained fluoride, showed clear radiopaque bands within the lesion. The bands, which occurred only in the presence of fluoride, appeared to be due to remineralization. When using an in vitro system that simulates the natural root caries process, it is imperative to understand the components of the particular model, as well as its limitations, and to be aware of the need for more than one evaluative technique.

Buffers↗
PubMed1978

Methods of preparation of fossil bone samples for light and transmission electron microscopy.

The methods described make possible the preparation of fossil samples for light nad transmission electron microscopy. To obtain good images with ground sections of bone, both the grinding process and classical bone staining techniques were modified, the latter by staining already made ground sections. Ground sections of suitable thickness were etched with a 4:1 mixture of 5% HNO3 and 2% OsO4 at a lowered temperature, thereby obtaining isolated osteocytes with a complete network of processes. Using the same method with thicker ground sections, the contents of vascular canals were isolated. The contents retained spatial relations permitting complete reconstruction of the vascular system of the bone in question. The isolated osteocytes, the vascular canal contents, and the isolated remnants of the intercellular organic part of this bone, subsequently embedded in Epon 812 and in methacrylate, made possible sections suitable for detailed analysis of the image of the surfaces of these elements. Polished surfaces of the fossil bone were replicated by the so-called clearing replicas technique adapted to fossil bone. The latter facilitated analysis of the ultrastructure of the surfaces of the vascular canals, isolated osteocytes and their processes, and analysis of the immediate proximity of these elements, since the technique preserves the mutual spatial relations intact.

Animals↗