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At least 73 records · Page 4Linked to original sources
PubMed1996

Variability and inheritance of double-stranded RNA viruses in Phaffia rhodozyma.

The present survey demonstrates polymorphism in both the length and the number of double-stranded RNAs (dsRNAs) among six Phaffia rhodozyma strains. Strains with one-, three- and four-types of dsRNA molecules were found, while two strains proved to be dsRNA-free. Elongated icosahedral virus-like particles (VLPs) 34x26 nm in size were detected in strains carrying four- or three-types of dsRNAs. One 3.7-kb dsRNA molecule was found not to form part of the VLP genome. Transmission of the VLPs of strain ATCC 24203 was followed through the basidiospores during the sexual cycle. Cytoplasmic inheritance was observed.

Basidiomycota↗
PubMed1975

Zygote heterogeneity and uniparental inheritance of mitochondrial genes in yeast.

A number of different crosses between strains of Saccharomyces cerevisiae differing in mitochondrial genotype are analyzed with respect to the extent to which individual zygotes transmit mitochondrial genes from one parent or the other. Many crosses produce two or more distinct classes of zygotes in this respect. Some crosses produce a high frequency of uniparental zygotes, which transmit mitochondrial genes exclusively or nearly so from one parent. Such zygotes cannot be accounted for in terms of an unequal input of mitochondrial DNA molecules from the two parents; they indicate that mitochondrial DNA from one parent is selectively replicated, or mitochondrial DNA from the other parent is selectively destroyed, in the zygote. Multiple zygote classes, and uniparental zygotes, are seen in studies of mitochondrial and chloroplast inheritance in other organisms, and may have a common explanation.

DNA, Mitochondrial↗
PubMed1975

Susceptibility of a hybrid plasmid to excision of genetic material.

A 5 Megadalton segment of DNA carrying a gene for kanamycin resistance from R447 b (a plasmid of group N of molecular weight 33 Megadaltons) has been inserted into Plac (a plasmid of the A--C complex of molecular weight 101 Megadaltons) to produce the recombinant plasmid Plac-R447 b (Coetzee, 1974). The recombinant plasmid is a typical member of the A--C complex except that entry of an N group plasmid into a Plac-R447 b+ recipient frequently leads to the loss of 5 Megadaltons of DNA (including the kanamycin resistance determinant) from the resident plasmid. In those transcipients from which kanamycin resistance is not eliminated, both plasmids are stably inherited.

Conjugation, Genetic↗
PubMed1976

Cytoplasmic inheritance in Saccharomyces cerevisiae: comparison of zygotic mitochondrial inheritance patterns.

Mitochondrial movements in Saccharomyces cerevisiae (Sc) zygotes were monitored with phase-contrast microscopy and compared to known mitochondrial inheritance systems. The mitochondria of Sc were convincingly identified by integrated use of phase-contrast, cytochemical and electron microscopic observations. Mitochondria in Sc appear to move by saltatory jumps, which appear to be oriented towards movement of mitochondria into developing buds. Tracking of mitochondria of different genotypes was made possible by positive identification of each mitochondrial population before zygosis, and by the low degree of mixing (less than 10%) of mitochondrial populations before first bud septation. A grande by grande cross demonstrated equal numbers of mitochondria from each haploid moving into the first zygotic bud. A grande by neutral petite cross gave a 2:1 ratio of grande to petite mitochondria. However, a grande by suppressive petite cross gave equal numbers of grande and petite mitochondria. Using drug resistance systems, a comparison was made of highly biased (97%) and moderately biased (71%) chloramphenicol resistant inheritance patterns. In both cases, the ratios of drug resistant to sensitive mitochondria were 1:1. When numbers of mitochondria moving into an individual bud were compared to the phenotypic content of the clone of that bud, no model could be constructed which could predict the latter from the former. The data indicate (with the exception of the neutral petite by grande cross) that the numbers of each mitochondrial type "inserted" into the first zygotic bud are equal, regardless of the degree of asymmetry of inheritance of mitochondrial markers.

Chloramphenicol↗