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Toxic effects of ingested lead shots in domestic fowls.

Lead poisoning from ingested shots is thought to be a major cause of high mortality in waterfowls throughout the world, and some millions of fowls die each year. However, there have been no other Japanese studies regarding lead toxicity in birds from ingested lead shots. We used domestic fowls instead of waterfowls as the experimental birds, in order to make clear the distribution and the toxic effects of lead shot in the birds. In a 1-wk follow-up study, two, four, and eight #4 lead shots were administered orally. A dose-dependent increase of the lead concentrations in blood, brain, liver, kidney, lung, spleen, bone, and epidermis of the gizzard was observed. In the 12-wk follow-up study, twenty domestic fowls were used and eight #4 lead shots were administered to the experimental birds. Lead concentrations in brain, liver, kidney, bone, ovary, fat tissue, and breast muscle increased more than in the 1-wk follow-up study. The observed lead concentrations of organs in the domestic fowls were lower than those of the other species used in past studies. The blood lead concentrations increased up to the third week and a remarkable suppression of delta-aminolevulinic acid dehydratase activity in red blood cells and elevation of free erythropoietic protoporphyrin were observed in the exposed group. Body weight loss, loss of hair, and neurological symptoms were also observed. However, there were no mortalities during the 1- and 12-wk studies.

Administration, Oral↗

Role of serotonin in the regulation of growth hormone and prolactin secretion in the domestic fowl.

Plasma levels of GH and prolactin were measured by radioimmunoassay in male domestic fowl treated with centrally active agents. p-Chlorophenylalanine (pCPA) did not have an effect on tonic levels of prolactin but led to a significant rise in circulating GH concentrations. The three serotonin receptor antagonists tested, methysergide, SQ-10631 and cyproheptadine, each resulted in a significant reduction in plasma prolactin while markedly increasing plasma GH levels. Administration of 5-hydroxytryptophan led to a rise in plasma prolactin and a drop in plasma GH levels in untreated birds or in animals pretreated with pCPA. The serotonin receptor agonist, quipazine, resulted in a marked increase in plasma prolactin and a marked reduction in plasma GH concentrations in untreated birds. In pCPA-pretreated animals quipazine was no longer effective in altering plasma prolactin levels but still caused a significant drop in circulating levels of GH. These results suggest that in the young male domestic fowl serotonin has a stimulatory role in the regulation of prolactin and an inhibitory role in the regulation of GH secretion.

5-Hydroxytryptophan↗

Effects of temperature and pH on osmotic fragility of erythrocytes of the domestic fowl (Gallus domesticus) and guinea-fowl (Numida meleagris).

The effects of temperature and pH on the osmotic behaviour of erythrocytes of the domestic fowl and guinea-fowl were studied. Erythrocytes of the guinea-fowl were observed to be more osmotically fragile than those of the domestic fowl at 29 degrees C and pH 7.7. The osmotic fragility of erythrocytes of both species decreased as the temperature (at constant pH of 7.7) or pH (at constant temperature of 29 degrees C) increased. The erythrocyte count, packed cell volume, haemoglobin concentration and mean corpuscular volume were identical in both species.

Animals↗

Purification and characterization of cholyl-CoA: taurine N-acetyltransferase from the liver of domestic fowl (Gallus gallus).

The enzymological basis for the ability of mammalian liver to conjugate bile acids with both glycine and taurine, and for non-mammalian liver to make only taurine conjugates, was investigated. The taurine-conjugating enzyme has been purified 1200-fold from the liver of domestic fowl and its properties compared with those of the glycine/taurine-conjugating enzyme from bovine liver [Czuba & Vessey (1980) J. Biol. Chem. 255, 5296-5299]. The enzyme from both species followed a Ping Pong mechanism. The enzymes were also similar with respect to their affinity for taurine, although the enzyme from domestic fowl would not bind glycine. The affinity of both for cholyl-CoA was quite similar, too, and both enzymes were inhibited reversibly by p-mercuribenzoate. The enzymes, however, were quite different in size. The enzyme from domestic fowl had a mol.wt. of 63000-65000 by both gel filtration and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. This is approx. 15 000 mol.wt. units larger than the enzyme from bovine liver, and suggests a loss of genome over the course of evolution as the basis for the altered specificity at the amino-acid binding site.

Acyl Coenzyme A↗

[Staphylococcus aureus infections in domestic fowls. 1. Ecology and epizootiology. (Review)].

The ecological and epizootiological aspects relating to Staphylococcus aureus in domestic fowl are expounded, on the basis of literature, with particular reference being made to five topics: occurrence of these conditionally pathogenic bacteria in domestic fowl, point of attack and routes of infection, conditions for possible occurrence of infections, reciprocal infection between man and animal, as well as aspects of therapy and prophylaxis.

Animals↗

Monophyletic origin and unique dispersal patterns of domestic fowls.

With the aim of elucidating in greater detail the genealogical origin of the present domestic fowls of the world, we have determined mtDNA sequences of the D-loop regions for a total of 21 birds, of which 12 samples belong to red junglefowl (Gallus gallus) comprising three subspecies (six Gallus gallus gallus, three Gallus gallus spadiceus, and three Gallus gallus bankiva) and nine represent diverse domestic breeds (Gallus gallus domesticus). We also sequenced four green junglefowl (Gallus varius), two Lafayette's junglefowl (Gallus lafayettei), and one grey junglefowl (Gallus sonneratii). We then constructed a phylogenetic tree for these birds by the use of nucleotide sequences, choosing the Japanese quail (Coturnix coturnix japonica) as an outgroup. We found that a continental population of G. g. gallus was the real matriarchic origin of all the domestic poultries examined in this study. It is also of particular interest that there were no discernible differences among G. gallus subspecies; G. g. bankiva was a notable exception. This was because G. g. spadiceus and a continental population of G. g. gallus formed a single cluster in the phylogenetic tree. G. g. bankiva, on the other hand, was a distinct entity, thus deserving its subspecies status. It implies that a continental population of G. g. gallus sufficed as the monophyletic ancestor of all domestic breeds. We also discussed a possible significance of the initial dispersal pattern of the present domestic fowls, using the phylogenetic tree.

Animals↗

A variant in the restriction endonuclease cleavage pattern of mitochondrial DNA in the domestic fowl, Gallus gallus domesticus.

The cleavage patterns of mitochondrial DNAs (mtDNAs) were investigated from 15 lines of domestic fowls, Gallus gallus domesticus, using 11 restriction endonucleases. The cleavage patterns with 10 restriction endonucleases were identical in all the lines. A variant was found in a line of White Leghorn in the pattern with MspI digestions. Cleavage patterns of the red jungle fowl, Gallus gallus gallus, were identical to the common patterns shown by the 14 lines of domestic fowls.

Animals↗

[Incidence of allergic reactions in small flocks of poultry and the pathogenesis of avian mycobacteriosis in domestic fowl].

Incidence of allergic reactions to avian tuberculin was investigated in 12,549 birds of the domestic fowl in the territory of one district, in 16 villages and in 721 small flocks. The reactions were found in 7.3% of birds and 35.5% flocks were infected. The zero reactions to tuberculin were observed only in one village out of the 16 villages investigated. Experimental infection confirmed high susceptibility of the domestic fowl to M. avium. After intramuscular implantation of M. avium suspension allergy to avian tuberculin was observed within a fortnight while within the same period histological lesions were found in muscle at the spot of puncture, in liver and spleen. Macroscopic lesions at the spot of puncture and in liver appeared in 21 days, and miliary tuberculosis of liver and spleen was detected almost regularly since day 28. Cultivation demonstrated mycobacteria in all the organs and tissues examined except the intestine in 12 days after infection. After peroral infection tuberculin allergy was observed in 106 days. Histological lesions were found in spleen in 49 days, as well as in liver, lungs and intestines in 96 days. Macroscopic lesions occurred in liver, spleen, lungs and intestine in 106 days. Small numbers of mycobacteria were isolated from various organs and tissues in 36 days after infection, and also from the intestine in 160 days. After infection by contacts with the tuberculous fowl tuberculin allergy appeared in 157 days. The first tuberculous lesions appeared in liver in 106 days, in spleen and intestine in 180 days.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

Plasma concentrations of somatomedin-C in hypophysectomized, dwarf and intact growing domestic fowl as determined by heterologous radioimmunoassay.

The application of a human somatomedin-C radioimmunoassay for the determination of somatomedin-C in chicken plasma has been examined. Parallel inhibition of binding of 125I-labelled somatomedin-C to antisera raised against somatomedin-C was observed with acid-treated human and chicken plasma. The concentration of immunoreactive (IR)-somatomedin-C in the plasma of the domestic fowl appears to be GH dependent. Plasma concentrations of IR-somatomedin-C were reduced after hypophysectomy and partially restored by replacement therapy with chicken GH. The age/development pattern of circulating concentrations of IR-somatomedin-C has been determined in normal and dwarf strains of domestic fowl. Increases in the plasma concentration of IR-somatomedin-C were observed between 1 and 6 weeks of age in control male domestic fowl of either heavy (broiler type) or light (White Leghorn) strains. Thereafter, the plasma concentrations of IR-somatomedin-C remained constant in the heavy strain birds but declined in White Leghorn chicks. Plasma concentrations of IR-somatomedin-C were reduced in sex-linked dwarf chickens, in both light and heavy strains of fowl, but were unaffected in autosomal dwarf chickens.

Animals↗

Immunoreactive neuropeptide systems in avian embryos (domestic mallard, domestic fowl, Japanese quail).

In embryos of the domestic mallard, domestic fowl, and Japanese quail vasotocin-, mesotocin-, luliberin (LHRH)-, met-enkephalin-, corticotropin-, and somatostatin-immunoreactive perikarya and fiber formations were visualized at different incubation stages by means of the PAP technique (Sternberger 1979). The most striking results were: (1) Vasotocin-, mesotocin-, and luliberin-immunoreactive systems display, up to the late embryonic period, morphological features most probably related to a neurohormonal function. (2) Met-enkephalin immunoreactivity appears very late during embryonic life; it is restricted to fiber networks and not found in perikarya. (3) Corticotropin immunoreactivity is observed in the tuberal region temporarily at the end of the second and the beginning of the last third of the incubation period. (4) Somatostatin-immunoreactive material is present (i) at the end of the first third of incubation, in association with the olfactory system; (ii) during the same period, adjacent to thin-layered portions of the roof of the brain; (iii) shortly thereafter, in cells of both pancreatic primordia and thyroid gland; and (iv) onward from the middle of the incubation period, in a mesencephalic cell group. The striking difference, in the early embryo, between the mature somatostatin plays a role in the development of the brain, as well as the pancreas, and the thyroid gland.

Adrenocorticotropic Hormone↗

Effects of methyl mercury at different dose regimes on eggshell formation and some biochemical characteristics of the eggshell gland mucosa of the domestic fowl.

Eggshell formation and egg production in domestic fowl were studied following the administration of methyl mercury (two dose regimes: 5 mg daily for 6 consecutive days and 1 mg daily for 50 consecutive days). A daily oral dose of 5 mg of methyl mercury for 6 consecutive days induced significant eggshell thinning and deformation and inhibited egg production. Uptake of 45Ca and synthesis of prostaglandins by a homogenate of eggshell gland mucosa from methyl-mercury-treated birds were significantly reduced, as was the calcium content of blood plasma. A daily oral dose of 1 mg of methyl mercury administered for 50 consecutive days also induced eggshell deformation and thinning and reduced egg production. This dose did not, however, have significant effects on the following: 45Ca uptake and prostaglandin synthesis by a homogenate of the eggshell gland mucosa; 45Ca uptake by a homogenate of duodenal mucosa; the Ca content of the blood plasma, shell gland mucosa or shell gland lumen; the HCO3- content of the shell gland lumen or the specific gravity of tibia. Methyl mercury added in vitro to a homogenate of eggshell gland mucosa significantly stimulated the synthesis of prostaglandins PGF2 alpha and PGE2. Addition of mercury chloride to the same type of preparation stimulated the synthesis of PGF2 alpha at the expense of thromboxane (TxB2) synthesis. Administration of 5 mg methyl mercury for 6 consecutive days seemed to reduce the availability of calcium for eggshell formation. This effect could have been due to a direct inhibitory effect of methyl mercury on calcium uptake from the gastrointestinal tract and/or to mobilization of medullary bone.(ABSTRACT TRUNCATED AT 250 WORDS)

Administration, Oral↗

Water diuresis in the domestic fowl.

1. The exteriorization of ureters in domestic fowls allows the collection of urine uncontaminated by faeces.2. When water is administered by stomach tube to a fowl, water is stored in the crop, from which it is slowly released into the proventriculus.3. Immediately after water gavage, there is a sharp rise of urine flow accompanied by an increase in endogenous creatinine excretion and a fall in the osmolarity of the urine. This initial increase in urine flow is of short duration, and is followed by a normal curve of diuresis with a peak at about 90 min after hydration.4. The first rise in urine flow following the filling of the crop appears to be of reflex origin, as distension of the crop with paraffin produces an enhanced excretion of urine and of creatinine.5. Changes in urine flow and in creatinine excretion are closely correlated.6. After water deprivation for 18 hr, the urine is slightly hyptertonic to blood. The maximum urine concentration observed was of the order of 600 m-osmole/l.

Animals↗

Central versus peripheral opioid regulation of ingestive behavior in the domestic fowl.

1. Three experiments were conducted to determine whether opioid regulation of ingestive behavior in the domestic fowl is mediated at sites within the central nervous system (CNS) or peripheral tissues. 2. Food and water intake were significantly decreased by the intramuscular (im) injection of naloxone hydrochloride (NHCl) and naloxone methobromide, which have a high and low ability, respectively, to cross the blood-brain barrier. 3. Water, but not food, intake was significantly decreased by the intracerebroventricular (ICV) injection of NHCl. However, water intake was not affected by the im injection of doses which were effective when given ICV. 4. These results suggest that in the domestic fowl there is a peripheral component to opioid regulation of food intake, while opioid regulation of water intake seems to be mediated at peripheral sites and within the CNS.

Animals↗

Alteration of food intake following intracerebroventricular administration of plasma from free-feeding domestic fowl.

Four experiments were conducted to investigate the presence of a factor in the plasma of the domestic fowl that alters food intake when injected into the lateral cerebral ventricle. Plasma collected from free-feeding and 24-hour fasted birds was lyophilized and reconstituted to 2, 4, or 5 times normal concentration. Stainless steel guide cannulae were stereotaxically implanted into the lateral cerebral ventricle of 8-week-old Single Comb White Leghorn cockerels. Free-feeding birds received 10 microliter intracerebroventricular (ICV) injections of normal (Experiment 1) and concentrated (Experiments 2, 3, and 4) plasma collected from free-feeding and 24-hour fasted birds. Artificial cerebrospinal fluid (aCSF) served as the control. Food intake was significantly decreased by ICV injections of 2, 4, and 5 times normal concentration of plasma from fed birds. Plasma from fasted birds did not alter food intake regardless of concentration, but did significantly reduce water intake when concentrated to five times normal. It appears that a satiety factor, which can be detected by the central nervous system, is present in the plasma of free-feeding domestic fowl.

Animals↗

Determination of testis temperature rhythms and effects of constant light on testicular function in the domestic fowl (Gallus domesticus).

There is a wide range of opinions regarding the operating temperature of the testis in the domestic fowl. We used physiological monitoring techniques to investigate testis and body temperature over daily periods and under various light regimes to elucidate body temperature gradients in the fowl. We confirm that the operating temperature of the adult fowl's testes is equivalent to core body temperature (40-41 degrees C). Long-term continuous temperature monitoring showed that there was no difference between the temperature of the testis, liver, and peritoneum during a 24-h period either in a normal light:dark cycle or under constant light conditions. However, there was a slight decrease in all temperatures at subjective night in each case, a decrease that does not appear to be sufficient to influence spermatogenesis. Birds maintained under constant light throughout two cycles of the seminiferous epithelium (28 days) still exhibited normal testis function and structure, even when "nightly" testis temperature decrease was the lowest. Thus, by undergoing spermatogenesis at an elevated temperature, the domestic fowl system is unique among the homeothermic animal systems studied to date.

Animals↗

A pineal ganglion associated with the pineal tract in the domestic fowl.

A ganglion-like aggregate consisting of acetyl-cholinesterase-positive neurons was demonstrated in the pineal organ of the domestic fowl by means of light and electron microscopy. This ganglion is located in juxtaposition with the pineal tract at the posterior (caudal) aspect of the pineal stalk. Numerous large and small neurons formed the ganglion in 40-day-old domestic fowl. Some of these nerve cells established direct neuro-neuronal contacts, others were surrounded by satellite cells. These ganglion cells displayed axo-somatic and axo-dendritic synapses. The above-mentioned cluster of nerve cells may be considered as a "pineal ganglion". It central or peripheral nature is open to discussion.

Animals↗

Branching of the coeliac artery in some domestic birds. iii. A comparison of the pattern of the coeliac artery in three breeds of the domestic fowl (Gallus gallus f. domestica).

The authors examined the pattern of the coeliac artery and its branches in 100 females of the domestic fowl of three breeds: White Leghorn (33 females), Brown Leghorn (34 females) and White Plymouth (33 females). The arterial pattern was injected by red-dyed latex. An exact analysis of the pattern of branches of the coeliac artery in the individual breeds was made, the individual signs were compared and the level of statistical significance in the differen breed-related findings was determined. On the basis of study of the material it is possible to state that: a) There exist certain differences in the pattern of branches of the coeliac artery in various breeds of the domestic fowl. b) These differences comprise especially the way of origin of the A. proventricularis dorsalis, the origin of spleen arteries, the existence of the accessory arteries for the gall bladder, bile ducts and the duodenum, the origin of the Aa. ileocaecales and the way of branching of the A. gastrica sin. c) It will be possible to compare the pattern of the coeliac artery in various species of birds no sooner than the breed-related differences are determined.

Animals↗

Early nuclear events of in vitro fertilization in the domestic fowl (Gallus domesticus).

In vitro fertilization in the domestic fowl (Gallus domesticus) was investigated by observation of the early nuclear events. Ova retrieved from the fimbria following ovulation were inseminated in vitro with 10(6)-10(7) spermatozoa in Dulbecco's modified Eagle's medium (DMEM) for 10 min and then further incubated in DMEM + albumen for 1, 2, 3, or 4 hr. These eggs were histologically examined by epifluorescent microscopy after staining with 4',6'-diamidino-2-phenylindole (DAPI). Nuclei of spermatozoa at various stages of transformation were observed in the ova incubated for 1-3 hr. Close pairing of two pronuclei, presumed to be male and female juxtaposition, was detected in ova incubated for 4 hr. These data provide direct evidence for the in vitro fertilization of fowl eggs and suggested that the early process of in vitro fertilization is comparable to that of in vivo fertilization.

Animals↗