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PubMed1983

Isoantibodies to epididymal sperm antigens in BALB/c strain of mice.

Female mice of the BALB/c isogenic strain were immunized with epididymal sperm of the male mice. Four antigenic preparations were used: whole sperm (WS), homogenized sperm (HS), protein fraction (PF) and lipid fraction (LF). Antisera were assayed for antibody activity by sperm immobilization and indirect immunofluorescence tests. The differential results between the WS and HS antisera obtained in the two tests suggest that sperm membrane surface antigen(s) may be distinct from intracellular antigen(s). PF-antiserum showed lower but significant activity with the sperm immobilization test but weak activity with the indirect immunofluorescence test. The lipid fractions showed extremely weak activities with both tests. Indirect immunofluorescent staining was carried out on 10 micrometer sections of unfertilized and fertilized eggs and 2-cell embryos. Unfertilized eggs showed no antibody binding and fertilized eggs showed patchy fluorescence of the plasma membrane and cytoplasm. In the 2-cell embryo the fluorescence was diffuse, suggesting spreading of the sperm antigens. Comparative electrophoresis of aliquots of HS antiserum with and without incubation with washed sperm showed a clear reduction, due to adsorption by sperm, of IgG class of antibodies, on the basis of molecular weight. The differences in antigenicity between the four sperm preparations are discussed in relation to (i) possible localization of the antigens in the spermatozoa and (ii) the conformational differences of the antigens manifested by organic solvent denaturation.

Animals↗
PubMedDate not supplied

The action of anti-A,AB isoantibodies on group A, AB-positive human lymphocytes.

Human lymphocytes of group A and group AB individuals were exposed to anti-A,B antisera both in growth medium and in fresh autologous plasma. Agglutination developed slowly but was present in all positive specimens by four hours and was negative in the group O controls even after 24 hours. There was no loss of viability even in the presence of the fresh plasma. The lymphocytes in the agglutinates appeared larger and more irregular suggesting stimulation of the lymphocytes but assessment by 3H thymidine uptake showed no evidence of a mitogenic response.

ABO Blood-Group System↗
PubMed1953

Experimental erythroblastosis fetalis in rabbits. II. The passage of blood group antigens and their specific isoantibodies across the placenta.

Female rabbits became immunized during pregnancy to the rabbit blood group factors G or g in five out of ten instances in which the red cells of the fetuses carried one of the factors absent in the mother. Antibodies so produced were of low titer and disappeared in all cases within 6 weeks after the birth of the litter. Repeated pregnancies did not result in additive increases in titer. Antibodies to the G-g factors, whether produced by the injection of red cells or by pregnancy, crossed the placenta readily from mother to fetus and were found at birth (and prior to nursing) associated with the red cells and in the serum of the fetuses. The rabbit placenta appeared to be equally permeable to the agglutinating and coating antibodies. The implications of these findings and their relation to the pathogenesis of erythroblastosis fetalis are briefly touched upon.

Animals↗