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Decomposition patterns in terrestrial and intertidal habitats on Oahu Island and Coconut Island, Hawaii.

Decomposition studies were conducted at two sites on the Island of Oahu, Hawaii, to compare patterns of decomposition and arthropod invasion in intertidal and adjacent terrestrial habitats. The animal model used was the domestic pig. One site was on Coconut Island in Kaneohe Bay on the northeast side of Oahu, and the second was conducted in an anchialine pool located at Barber's Point Naval Air Station on the southwest shore of Oahu. At both sites, the terrestrial animal decomposed in a manner similar to what has been observed in previous studies in terrestrial habitats on the island of Oahu. Rate of biomass depletion was slower in both intertidal studies, and decomposition was primarily due to tide and wave activity and bacterial decomposition. No permanent colonization of carcasses by insects was seen for the intertidal carcass at Coconut Island. At the anchialine pool at Barber's Point Naval Air Station, Diptera larvae were responsible for biomass removal until the carcass was reduced below the water line and, from that point on, bacterial action was the means of decomposition. Marine and terrestrial scavengers were present at both sites although their impact on decomposition was negligible. Five stages of decomposition were recognized for the intertidal sites: fresh, buoyant/floating, deterioration/disintegration, buoyant remains, and scattered skeletal.

Animals↗

[Conclusions of the Canary Islands Nutrition Survey (1997-98). Foundations for a nutrition policy in Canary Islands].

The principal findings of the Canary Island Nutrition survey (ENCA). 1997-98 are presented. This survey was realised to obtain the following objectives: to evaluate eating habits and nutritional status utilising dietary, anthropometric and biochemical indicators in the Canary Island population along with determining lifestyle factors and the prevalence of the main cardiovascular risk factors. Additionally, a survey evaluating food-related knowledge and attitudes was also carried out. To attain this end, a cross-sectional study was realised in a representative sample of the Canary Island population aged 6 to 75 years (n = 1,747). This article summarises the key findings related to study objectives as well as study-derived recommendations to be realised within a Canary Island health and nutrition policy framework. For this purpose, the development of food based dietary guidelines addressed to meet target nutrients is needed.

Adolescent↗

[The lateral abdominal island flap--the lateral intercostal neurovascular island flap].

This work studied the lateral abdominal island flap, its clinical value, transposition range and the practicability of a modified operative method. Five lateral abdominal island flaps were used in 5 patients. Four of them were for axillary radiation ulcers after radical mastectomy. One was for a sacral defect after resection of a recurrent fibrosarcoma. All the flaps obtained satisfactory results. Clinical applications revealed that the blood supply of the lateral abdominal skin was mainly from the lateral cutaneous branches of the 9th, 10th and 11th intercostal arteries, among which there were numerous anastomoses. The lateral abdominal island flap can be pedicled with any of these lateral cutaneous branches. The long pedicle of the flap provides a wide range of trnasposition from the axilla to the sacrum. As the pedicle of the flap contains the lateral cutaneous branch of the intercostal nerve, the flap can bring sensation function to the recipient area. The modified operative method of the lateral abdominal island flap is introduced.

Adult↗

The comparative hydrochemistry of two granitic island aquifers: the Isles of Scilly, UK and the Hvaler Islands, Norway.

A comparative study is presented of granitic groundwaters from the Hvaler Islands, south-eastern Norway (11 samples) and the Scilly Islands, south-western England (10 samples). The islands display similar bulk lithologies (peraluminous S-type, U/Th-enriched granites) and land use, but differing glaciation and hence weathering histories. The groundwater of both groups bears a strong marine signature, although the Hvaler Islands display less marine influence and a greater degree of water-rock interaction. The most interesting hydrochemical dissimilarities concern the health related trace elements Rn, U and F. These display median (and maximum) values of 2510 Bq/l (8520 Bq/l), 15 micrograms/l (170 micrograms/l) and 3.3 mg/l (4.4 mg/l), respectively, for Hvaler, compared with 140 Bq/l (200 Bq/l), 1.5 micrograms/l (4 micrograms/l) and 0.1 mg/l (0.27 mg/l) for Scilly. Commonly employed drinking water limits for these parameters are 500 Bq/l (Norwegian action level), 20 micrograms/l (Canadian limit) and 1.5 mg/l. The differences in groundwater contents of these elements between Hvaler and Scilly may be ascribed to: (i) differing trace element compositions of the granites and fracture mineralisations; (ii) radically differing recent weathering histories; and (iii) hydrodynamic factors.

Chemical Phenomena↗

MtDNA phylogeography and recent intra-island diversification among Canary Island Calathus beetles.

The genus Calathus Bonelli comprises 24 species on the Canary Islands. Sequences of 927 and 687 bp of the mitochondrial cytochrome oxidase I and II genes, respectively, as well as the intervening tRNA leu gene in 21 of the 24 species, have identified three genetically divergent and unequivocally monophyletic groupings. A phylogeographic analysis is presented for the major monophyletic group comprising all the species of Gran Canaria, La Gomera, and El Hierro, and two Tenerifean species. A distance-based phylogenetic analysis and maximum parsimony analysis have clearly shown that this clade is composed of four distinct lineages. DNA sequence data suggest a recent origin for this clade and that lineages have not evolved at the same rate. Compared with diversification patterns observed in other Coleoptera on the Canary Islands, diversification has been recent relative to the time of colonization within the islands of Gran Canaria and La Gomera. Calathus diversification on La Gomera has been greater than on Gran Canaria. The influences of geological and ecological history are discussed in relation to Calathus diversification.

Animals↗

Mitochondrial DNA sequence variation and phylogeography of Pimelia darkling beetles on the island of Tenerife (Canary Islands).

Four morphological taxa of the beetle genus Pimelia (Coleoptera, Tenebrionidae) are known to exist on the Atlantic island of Tenerife. We have obtained DNA sequences for 61 individuals from these taxa across the island for a 200 bp long fragment of the mitochondrial COI gene. In addition, a restriction site polymorphism in the nuclear rRNA ITS-1 sequence was identified and screened in a sample of these individuals using the enzyme Kpn2I. The results were analysed using approaches which allow inferences to be made about the population genetic structure and the mitochondrial genealogy of these closely related beetles. The mtDNA haplotype distribution and the estimates of sequence divergence revealed the presence of two ancient mtDNA lineages which coincide with the disjunct volcanic evolution of the island. The ITS-1 polymorphism was found to be diagnostic of these two lineages. However, the morphological and mitochondrial phylogenies were found to be discordant. We argue that this is possibly the result of rapid morphological change, produced by selection in different habitats, which has been recently superimposed on an older mitochondrial DNA divergence.

Animals↗

Orofacial analysis on the Adriatic islands: an epidemiological study of malocclusions on Hvar Island.

The prevalence of malocclusion was studied on the Hvar island, Croatia. This island is characterized by high rates of endogamy, inbreeding and reproductive isolation. The sample for this study comprised 224 children, 126 males and 98 females from all schools on the island of Hvar (20.2 per cent of the total public school population). The sample was selected according to age, sex distribution, and demographic characteristics. The Angle Classes, overjet, overbite, crowding, spacing, and the type of anterior bite were examined. Class I buccal relationship was found in 47.3 per cent of the subjects, Class II in 45.1 per cent, and Class III in 5.4 per cent. Normal overjet was the most frequent finding (69.9 per cent). Normal overbite was seen in 42.5 per cent, deep in 49.1 per cent, and very deep in 2.7 per cent of the cases. Open bite was diagnosed in seven subjects (3.1 per cent) and edge-to-edge bite in six (2.7 per cent). The frequency of crowding was 57.1 per cent, normal relationships were observed in 34.9 per cent, and spacing in 8 per cent. The results of this investigation showed that the inhabitants of Hvar, who are characterized by a high rate of inbreeding and traditional diet, have more Class II malocclusions, deep bites, and midline shifts than the general Croatian population but not higher than other modern urbanized groups. Since the lack of chewing stress in this population cannot be considered as the main explanation for the above phenomenon, the genetic influence on the development of these occlusal traits in the inbred population of Hvar requires further investigation.

Adolescent↗

Evidence for a hybrid genomic island in verocytotoxin-producing Escherichia coli CL3 (serotype O113:H21) containing segments of EDL933 (serotype O157:H7) O islands 122 and 48.

Genomic O island 122 (OI-122) of the verocytotoxin-producing Escherichia coli (VTEC) strain EDL933 contains four putative virulence genes, Z4321, Z4326, Z4332, and Z4333. However, strain CL3 (serotype O113:H21) contains only Z4321, not the other three genes. To determine whether Z4321 is part of a different genomic island in CL3, a region of 27,293 bp up- and downstream of Z4321 was sequenced and found to contain elements of two different EDL933 genomic islands (OI-48 and OI-122) and a Yersinia pestis-like hemolysin/adhesin gene cluster. The region contained OI-48 genes Z1635, Z1636, and Z1637 at the left terminus and Z1641, Z1642, Z1643, and Z1644 at the right. The middle portion consisted of OI-48 gene Z1640, which was separated into three fragments by genomic segments including the Y. pestis cluster and EDL933 OI-122 genes Z4322, Z4321, and Z4318. In a PCR investigation of 36 VTEC strains of different serotypes, intact Z1640 was present in strains of serotypes O157:H7, O26:H11, O103:H2, O111:NM, and O145:NM, which are associated with hemolytic uremic syndrome and outbreaks. In contrast, fragmented Z1640 was seen in strains of nonepidemic serotypes, such as O91:H21 and O113:H21, and in animal serotypes that have not been associated with human disease, indicating that Z1640 might be a virulence gene.

Adhesins, Escherichia coli↗

Human enzyme polymorphism in the Canary Islands. IV. Eastern islands.

The genetic polymorphism of eight red cell enzymes was analyzed in four population samples from the two easternmost islands of the Canary Archipelago. No heterogeneity was detected within the islands, but differences between them were of the same magnitude as those found between the islands and the Spanish mainland. The presence of the African G6PD-A+ and the rare G6PD-Gc alleles seems to be a characteristic of all Canarian populations studied.

Alleles↗

Aboriginal and Torres Strait Islander health: current status and recent initiatives. Health and Community Development Branch, Aboriginal and Torres Strait Islander Commission.

Against a background of the appalling health problems experienced by indigenous Australians, the following discussion details the current status of health services for Aboriginal and Torres Strait Islander peoples and recent government and non-government initiatives to address these problems. In many localities, health services are overwhelmed by the tasks confronting them. State and Territory governments, having primary responsibility for the health of all citizens, must act in close cooperation with local communities, the Aboriginal and Torres Strait Islander Commission (ATSIC), regional councils and Aboriginal health services to ensure inequalities are addressed in health service provision to Australia's indigenous people. The Federal government, through ATSIC and the Department of Human Services and Health, has implemented a range of initiatives in order to make a significant contribution to indigenous health and welfare. A primary task remains to implement broader scale changes relevant to Aboriginal and Torres Strait Islander people within the mainstream health services. Mainstream services have the primary responsibility, and are funded on behalf of the whole population, to address health problems in a working partnership with the communities they serve. The Australian Hospital Association and its membership can help to improve the health of indigenous peoples by actively and sensitively working with them in the localities where they live, and through advocacy in the policies and plans that shape the whole of our health system.

Acquired Immunodeficiency Syndrome↗

The Pacific Island Health Care Project: easing the cancer burden in the United States associated Pacific Islands.

Cancer is a serious and ever increasing problem in the United States Associated Pacific Islands (USAPIs). Nearly 30% of all consultations and referrals are for cancer. Depending on the type of cancer, the prognosis for long-term survival is often poor and the cost of caring for such patients is considerable. Tripler Army Medical Center (TAMC) provides tertiary medical care to patients from the USAPIs in support of Graduate Medical Education (GME) at no cost to the patient or jurisdiction. Continuing its tradition of leading telemedicine initiatives in the Pacific, TAMC has developed a web-based electronic consultation and referral network, the Pacific Island Health Care Project (PIHCP). Ten sites in Micronesia and one in American Samoa were provided with computer equipment. Local clinicians request consultation from TAMC specialists through the internet, attaching supporting imagery. The consults are posted on a secure web page, where they are viewed, evaluated, forwarded, and commented on by the consultants. Experience with more than 2,100 cases indicates that the Internet can be easily used as a store-and-forward consultation format and offers a cost effective means for distance consultation, referral, and learning. This format has provided for a more rational evaluation of all patients but especially patients with cancer. Patients who are terminally ill, have widely metastasized disease, and/or have inoperable tumors or recurrences are excluded so that limited resources can be used for those most likely to benefit. There has been overwhelming enthusiasm for such Internet consultations between referring physicians and consultants. Many Pacific Islander patients have received state-of-the-art medical care through this program. At the same time, the training of TAMC physicians and residents has been enhanced by their opportunity to care for these remarkable and deserving patients from the Pacific.

Education, Medical, Continuing↗

Identification of the physiological promoter for spinocerebellar ataxia 2 gene reveals a CpG island for promoter activity situated into the exon 1 of this gene and provides data about the origin of the nonmethylated state of these types of islands.

In order to further use the spinocerebellar ataxia 2 (SCA2) promoter for transgenic mice models of "CAG repeat" neurodegeneration, different fragments of this 5' end were ligated into pGL3-Luc plasmid to obtain the better promoter-activity of the physiological promoter for SCA2. Base-par composition of the SCA2-5' region, and promoter prediction algorithms such as TSSW and TSSG, together with the high firefly luciferase expression after 48 hours of transient transfection in mammalian cells lines, showed a typical CpG island for promoter-activity. The promoter activity was specifically localized into the exon 1 of the SCA2 gene. The higher expression of firefly luciferase in the embryonal F9 cells by the use of SCA2 promoter, rather than by the use of CMV promoter may be related with the origin of the nonmethylated CpG island during the early embryogenesis. Analysis of the 5' region from HD gene revealed to a CpG island, which could be containing the physiological promoter for this gene.

Algorithms↗

Trace elements intake in the Faroe Islands. III. Element concentrations in human organs in populations from Bergen (Norway) and the Faroe Islands.

Flameless as well as flame atomic absorption spectrophotometry were used for the analysis of six elements (calcium, iron, zinc, selenium, cadmium and mercury) in human organs (liver, kidney cortex and medulla, heart, pancreas and spleen) from 13 bodies from Bergen and 10 from the Faroe Islands. Samples were taken at autopsy and the organs selected were without pathological signs. All patients were born between 1899 and 1923. Element concentrations in the organs studied were comparable to previous studies, except for high mercury and selenium values in the liver, the kidney cortex and medulla of subjects from the Faroe Islands. The high mercury and selenium values may be explained by the high consumption of pilot whales by the Faroe Islands population.

Aged↗

An island within an island: genetic differentiation of Anopheles gambiae in São Tomé, West Africa, and its relevance to malaria vector control.

Islands are choice settings for experimental studies of vector control strategies based on transgenic insects. Before considering this approach, knowledge of the population structure of the vector is essential. Genetic variation at 12 microsatellite loci was therefore studied in samples of the malaria vector Anopheles gambiae s.s., collected from six localities of São Tomé island (West Africa). The objectives were (i) to assess the demographic stability and effective population size of A. gambiae from these sites, (ii) to determine population differentiation and (iii) to relate the observed patterns of population structure with geographic, ecological and historical aspects of the vector on the island. Significant population differentiation, revealed by FST and RST statistics, was found between the southernmost site, Porto Alegre, and northern localities. The observed patterns of population substructure are probably a result of restrictions to gene flow in the less inhabited, more densely forested and mountainous south. In all localities surveyed, A. gambiae appeared to be experiencing a demographic expansion, consistent with a relatively recent (ca. 500 years) founder effect. The results are discussed with respect to current and future prospects of malaria vector control.

Africa, Western↗

High prevalence of the very rare Wilson disease gene mutation Leu708Pro in the Island of Gran Canaria (Canary Islands, Spain): a genetic and clinical study.

The molecular basis of Wilson disease (WD), an autosomal recessive disorder, is the presence of mutations in the ATP7B gene, a copper transporting ATPase. Hospital records indicated a higher prevalence of WD (1 in 2,600) in some counties in the northeastern region of the island of Gran Canaria (Canary Islands, Spain) that was around 10-fold higher than that described for European populations (1 in 30,000). The ATP7B gene was analyzed for mutations in 24 affected subjects, revealing a high prevalence of the rare Leu708Pro mutation present in 12 homozygous and 7 heterozygous individuals. In these patients, who constitute one of the largest described cohorts of WD homozygotes, we found a variable clinical presentation of the disease, although the biochemical picture was homogenous and characteristic, thereby confirming that the Leu708Pro change is indeed a mutation associated with WD. Haplotype analysis of subjects homozygous for the Leu708Pro mutation showed a conserved shared region smaller than 1 centimorgan (cM), and the region of linkage disequilibrium between the Leu708Pro mutation and neighboring microsatellite markers extended approximately 4.6 cM. When comparing the amount of linkage disequilibrium versus genetic distance from the disease mutation, it was estimated that a common ancestral Leu708Pro chromosome may have been introduced in Gran Canaria over 56 generations ago, dating it back to pre-Hispanic times. The prevalence, and the tight geographical distribution of the Leu708Pro chromosome suggests that the Canary Islands can be considered a genetic isolate for linkage disequilibrium studies.

Adenosine Triphosphatases↗

Comparison of faunal equilibrium turnover rates on a tropical island and a temperate island.

Rates of immigration and extinction of bird species on a tropical island, Karkar in the southwest Pacific Ocean, have been estimated from surveys made in 1914 and in 1969. Compared to a temperate-zone island of similar size and isolation (Santa Cruz off southern California), Karkar has a similar extinction rate, but a lower immigration rate expressed as a fraction of the mainland species pool, due to the sedentariness of many tropical forest birds. The probability of extinction is highest for species that are rare (due to narrow habitat requirements, large territory size, competition, recency of colonization, or marginal suitability of habitat), species with "in-and-out" tactics, and populations on small islands.

Journal Article↗

An Outbreak of Vibrio cholerae O1 infections on Ebeye Island, Republic of the Marshall Islands, associated with use of an adequately chlorinated water source.

In December 2000, physicians in the Republic of the Marshall Islands reported the first known outbreak of Vibrio cholerae O1 infection (biotype El Tor, serotype Ogawa) from this country. In a matched case-control study on Ebeye Island, patients with cholera (n=53) had greater odds than persons without cholera (n=104) to have drunk adequately chlorinated water collected from a US military installation on neighboring Kwajalein Island and transported back to Ebeye (matched odds ratio [MOR], 8.0; P=.01). Transporting or storing drinking water in a water cooler with a spout and a tight-fitting lid was associated with reduced odds of illness (MOR, 0.24; P<.01), as was drinking bottled water (MOR, 0.08; P<.01), boiled water (MOR, 0.47; P=.02), or water flavored with powdered drink mixes (MOR, 0.18; P<.01). No cases of cholera were reported among Kwajalein residents. This outbreak highlights the critical importance of handling and storing drinking water safely, especially during outbreaks of gastrointestinal illness.

Adolescent↗

Comparative study of reverse flow island flaps in the lower extremities--peroneal, anterior tibial, and posterior tibial island flaps in 25 patients.

The reverse flow island flap is one of the most versatile reconstructive procedures in the lower extremity. There are three major arteries, the peroneal, the anterior tibial, and the posterior tibial artery, and a reverse flow island flap pedicled by each vessel and its intermuscular cutaneous perforators is available. Twenty-five reverse flow island flaps were clinically applied for soft tissue defects in the lower leg (10 peroneal, 8 anterior tibial, and 7 posterior tibial flaps). We report a comparative study of the characteristics and indications of the peroneal, anterior tibial, and posterior tibial reverse flow flaps. We conclude that the anterior tibial reverse flow flaps are more likely, without venous anastomosis, to become congested and necrose than the peroneal and posterior tibial flaps.

Adult↗