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At least 73 records · Page 4Linked to original sources
PubMed2002

High-resolution hard X-ray phase-contrast microscopy with a large-diameter and high-numerical-aperture zone plate.

An imaging transmission hard X-ray microscope has been constructed at beamline BL24XU of SPring-8. It makes use of a phase zone plate made of tantalum with a diameter of 1 mm and an outer-most zone width of 50 nm, aiming at a wide field of view and a high spatial resolution. The performance test was carried out at a photon energy of 10 keV. A field of view as wide as approximately 200 microm in diameter was achieved. The spatial resolution was measured to be 220 nm by analyzing a knife-edge image. Further, a line-and-space pattern as fine as 100 nm could be imaged. By placing a phase plate made of gold in the back focal plane of the zone plate, phase-contrast microscopy using Zernike's method was also carried out. The feasibility of phase-contrast microscopy for observing transparent samples was successfully demonstrated by imaging small polystyrene particles.

Journal Article↗
PubMed1975

Detection of fungi in clinical specimens by phase-contrast microscopy.

During 1973 and 1974, the following fungi were detected in clinical specimens by using phase-contrast microscopy: Blastomyces dermatitidis, 5; Coccidioides immitis, 3; Cryptococcus neoformans, 11; other yeasts 918; dermatophytes, 863; Mucor species, 1; and Aspergillus fumigatus, 16. This technique allows rapid detection and, in many instances, immediate identification of fungi in clinical specimens.

Arthrodermataceae↗
PubMed1992

Comparative assessment of phase contrast microscopy and Coulter counter measurements in localising the site of haematuria.

Haematuria is a common diagnostic problem in clinical practice and is seen in a large number of renal, urological and systemic disorders. The routine use of dipstick methods has increased the frequency of detection of microscopic haematuria. It is desirable to develop a simple, non-invasive, easily reproducible, quantitative technique that would help to determine the site of bleeding and thereby direct the patient either to the nephrologist or to the urologist. This study compares the accuracy of phase contrast microscopy with Coulter counter measurement of red cell distribution volumes as a means of distinguishing glomerular haematuria from non-glomerular haematuria. Of 20 patients with biopsy-proven glomerulonephritis, 13 (65%) were correctly identified by phase contrast microscopy and 19 (95%) by the Coulter counter method. In 18 patients with pathological conditions affecting the lower urinary tract phase contrast microscopy was correct in 6 (33%) and Coulter counter measurement in 17 (94%). Coulter counter measurement is the simpler and more accurate method of screening for the source of haematuria, but is inaccurate when the red cell count in the urine is less than 0.02 x 10(12).

Adolescent↗
PubMed1969

Scanning electron and phase-contrast microscopy of bacterial spores.

The three-dimensional immages of free and intrasporangial spores produced by scanning electron microscopy show surface structures not visible by phase-contrast microscopy. Although fine surface detail is not elucidated by scanning electron microscopy, this technique does afford a definitive picture of the general shape of spores. Spores of Bacillus popilliae, B. lentimorbus, B. thuringiensis, B. alvei, B. cereus, and Sarcina ureae have varying patterns of surface ridge formation, whereas spores of B. larvae, B. subtilis, and B. licheniformis have relatively smooth surfaces.

Bacillus↗