[Description of a new microtome for living tissues].
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We present plans for an original holder for long-edged glass knives (Ralph knives). Knives are prepared by hand-breaking of commercial window glass (3 mm thick), for cutting large blocks of tissue embedded in a water-miscible plastic resin, glycol methacrylate.
Three methods are evaluated for holding capsules of plastic-embedded tissue for rotary microtomy. Use of a V-block is rapid but deforms the capsule. Gluing the capsule to a supporting block is useful for reorientation of the capsule but otherwise time consuming. adapter is easy to use, does not deform the tissue, and is the preferred method for routine microtomy.
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