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PubMedDate not supplied

Sensory analysis of polystyrene packaging material taint in cocoa powder for drinks and chocolate flakes.

Polystyrene packaging material taint was sensorily evaluated in cocoa powder for drinks and chocolate flakes using short-cut signal detection measures on differences between control and test samples and on recognition of styrene. No differences were observed in cocoa powder for drinks and plain chocolate flakes treated with 0.5 dm2 polystyrene of 1 mm thickness. However, differences were detected in milk chocolate flakes and plain chocolate flakes, which were in contact with a larger area or thicker polystyrene packaging material. The latter results were confirmed by the styrene recognition test, so polystyrene is a potential source of off-flavour for chocolate products. The amount of residual styrene in the polystyrene used was about 320 ppm, while the amounts of styrene ranged from 7 to 132 ppb in cocoa drinks and from 414 to 1447 ppb in chocolate flakes.

Cacao↗

PubMedDate not supplied

Differences in the adsorption behaviour of poly(ethylene oxide) copolymers onto model polystyrene nanoparticles assessed by isothermal titration microcalorimetry correspond to the biological differences.

The adsorption behaviour of a tetrafunctional copolymer of poly (ethylene oxide)-poly (propylene oxide) ethylene diamine (commercially available as Poloxamine 908) and a diblock copolymer of poly (lactic acid)-poly (ethylene oxide) (PLA/PEG 2:5) onto a model colloidal drug carrier (156 nm sized polystyrene latex) is described. The adsorption isotherm, hydrodynamic thickness of the adsorbed layers and enthalpy of the adsorption were assessed. The close similarity in the conformation of the poly (ethylene oxide) (PEO) chains (molecular weight 5,000 Da) in the adsorbed layers of these two copolymers was demonstrated by combining the adsorption data with the adsorbed layer thickness data. In contrast, the results from isothermal titration microcalorimetry indicated a distinct difference in the interaction of the copolymers with the polystyrene colloid surface. Poloxamine 908 adsorption to polystyrene nanoparticles is dominated by an endothermic heat effect, whereas, PLA/PEG 2:5 adsorption is entirely an exothermic process. This difference in adsorption behaviour could provide an explanation for differences in the biodistribution of Poloxamine 908 and PLA/PEG 2:5 coated polystyrene nanoparticles observed in previous studies. A comparison with the interaction enthalpy for several other PEO-containing copolymers onto the same polystyrene colloid was made. The results demonstrate the importance of the nature of the anchoring moiety on the interaction of the adsorbing copolymer with the colloid surface. An endothermic contribution is found when an adsorbing molecule contains a poly (propylene oxide) (PPO) moiety (e.g. Poloxamine 908), whilst the adsorption is exothermic (i.e. enthalpy driven) for PEO copolymers with polylactide (PLA/PEG 2:5) or alkyl moieties.

Adsorption↗

PubMed2000

Efficacy of the cation exchange resin, sodium polystyrene sulfonate, to decrease iron absorption.

BACKGROUND: Iron is not bound by charcoal; therefore, a method of binding iron in the gastrointestinal tract to prevent absorption in iron overdose is needed. This study investigated the efficacy and safety of sodium polystyrene sulfonate to prevent absorption of iron in human volunteers. METHODS: Six adult volunteers completed this prospective crossover trial. Following an oral dose of elemental iron 10 mg/kg, each subject received sodium polystyrene sulfonate 30 g or water as control. Baseline and serial serum iron samples were drawn to determine pharmacokinetic parameters. RESULTS: A trend toward increased time to peak following sodium polystyrene sulfonate compared to the control arm (5.7 vs 3.6 hours) was observed but was not statistically significant (p = 0.517). A trend toward smaller area-under-the-curve for the sodium polystyrene sulfonate was evident but was not statistically significant (p = 0.77). Iron concentration increased on average 298 mcg/dL and 370 mcg/dL above baseline in the treatment and control arms (p = 0.44). Sodium polystyrene sulfonate is not an effective method of decontamination for iron overdose.

Administration, Oral↗

PubMed2005

The effect of radio-frequency glow discharge treatment of polystyrene on the behavior of porcine chondrocytes in vitro.

The aim of this study was to determine the effects of physicochemical surface properties of tissue-culture substrata on chondrocyte behavior. Polystyrene was modified by radio-frequency glow discharge (RFGD) plasma treatment with various monomers. The changes in surface properties of the modified polystyrene were verified by ESCA and water contact angle measurements. Porcine chondrocytes were seeded on these surfaces and cultured for 5 days. After 5 days of culture, the number of chondrocytes was highest on the N2 plasma-treated surface, followed by the CH2/N2 plasma-treated surface, untreated polystyrene and CF4 plasma-treated surface. The number of chondrocytes decreased with increasing water contact angle. The surface chemical properties influenced the morphology and gene expression of cultured chondrocytes. The cells cultured on the CF4 plasma-treated surface retained a round morphology characteristic of chondrocytes after day 1, while most of the cells grown on the N2 plasma-treated surface or the untreated polystyrene showed a flattened morphology. Using RT-PCR, expression of type-I collagen could not be detected in the chondrocytes cultured on the CF4 plasma-treated surface and the CH2/N2 plasma-treated surface. In contrast, the chondrocytes grown on the N2 plasma-treated surface or the untreated polystyrene surface expressed type-I collagen mRNA. This study shows that modification by RFGD treatment could modulate chondrocyte culture and gene expression.

Acetylene↗

PubMed1996

Sodium polystyrene sulfonate (kayexalate) aspiration: histologic appearance and infrared microspectrophotometric analysis of two cases.

OBJECTIVE: Sodium polystyrene sulfonate (kayexalate) is a cation-exchange resin given enterally for the treatment of hyperkalemia. Aspiration of this material is a rare occurrence, but when visualized in the alveolus, it has a characteristic microscopic appearance that is virtually diagnostic. In two cases, recognition of the characteristic morphology of the foreign material raised the question of sodium polystyrene sulfonate. DESIGN: We used infrared spectroscopy to demonstrate the presence of this material in lung biopsies of two patients by identifying foreign body particles. Histories of the patients were reviewed for exposure to sodium polystyrene sulfonate. SETTING: Two lung specimens were referred to the Armed Forces Institute of Pathology with an unknown foreign material identified within the air spaces. PATIENTS: The lung biopsies were from two children, one postterm female infant who died at 3 days of life and a 4-year-old girl who underwent lung biopsy during surgical repair for tetralogy of Fallot. Both patients had received sodium polystyrene sulfonate previously for control of hyperkalemia. RESULTS: The lung specimens showed characteristic basophilic, amorphous foreign material in airspaces on histologic sections. The identity of this material was confirmed by Fourier transform infrared microspectrophotometry. CONCLUSIONS: To our knowledge, we report the first two cases of sodium polystyrene sulfonate aspiration in children. This material has a distinctive morphologic appearance on histologic sections, and its identity can be confirmed by Fourier transform infrared microspectrophotometry.

Cation Exchange Resins↗

PubMed1989

Cytotoxicity due to a reaction between polystyrene and constituents of stool.

Cell monolayers grown in polystyrene culture plates and in glass tubes are routinely used in virology laboratories around the world for virus isolation and identification. Monolayers of different types of cells grown in polystyrene culture plates were found to show a strange cytotoxicity when inoculated with stool samples from healthy children from Bangladesh. When inoculated with 0.2 ml of a 10% stool sample 100% (80/80) of the cell monolayers established in polystyrene plates showed cytopathy by 4 days and 60% (48/80) within first 2 hours of sample inoculation. In contrast only 4% (4/90) of samples inoculated in the same type of cell monolayers but established in glass cell culture tubes showed cytopathy by 4 days; out of these 4 only 2 (2%) showed cytopathy in the first 2 hours. Cytotoxicity could be reduced to half when the amount of inoculated stool sample was reduced by half in cases where polystyrene was used as the substrate. This difference of cytopathy under two different circumstances (polystyrene substrate versus glass substrate) was statistically significant (P less than 0.001).

Cell Survival↗

PubMed1996

Serotyping of Actinobacillus pleuropneumoniae serotype 5 strains using a monoclonal-based polystyrene agglutination test.

A polystyrene agglutination test has been developed for serotyping Actinobacillus pleuropneumoniae serotype 5a and 5b strains. Protein A-coated polystyrene microparticles were sensitized with a murine monoclonal antibody recognizing an epitope on serotype 5 LPS-O chain as shown by SDS-PAGE and Western blotting. A total of 205 A. pleuropneumoniae, strains including all 12 serotype reference strains and 13 strains representing 8 common bacterial species associated with swine or related to A. pleuropneumoniae, were tested by mixing 25 microL of polystyrene reagent with the same volume of a dense suspension of bacterial cells grown for 18 h. All A. pleuropneumoniae strains had been previously serotyped using standard procedures. The polystyrene agglutination test was rapid (less than 3 min) and easy to perform. Overall a very good correlation (97.3%) with the standard techniques was found. The sensitized polystyrene particles were stable for at least 6 mo.

Actinobacillus Infections↗

PubMed1996

Simple and more sensitive immune complex transfer enzyme immunoassay for antibody IgG to reverse transcriptase of HIV-1 using microplates, modified polystyrene solid phase and fluororeader.

In a previously reported ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for antibody IgG to HIV-1, polystyrene beads in test tubes were handled with tweezers, and bound beta-D-galactosidase activity was measured with a fluorometer. The use of tweezers was causative of false-positivity by carryover, and testing many samples was difficult. Recently, these drawbacks have been minimized using microplates, a fluororeader, and modified polystyrene beads with sticks for easy handling. The modified polystyrene beads were transferred from wells to wells more quickly and easily without tweezers, eliminating false-positivity due to carryover. The fluorescence intensity for bound beta-D-galactosidase activity was quickly measured with a fluororeader. As a result, it became easy to test many samples with high reliability. However, modified polystyrene beads used were handmade, and the sensitivity was lower than the previous assay with test tubes. In the present study, the condition of the immune complex transfer enzyme immunoassay for antibody IgG to reverse transcriptase of HIV-1 was optimized using microplates, a fluororeader, and polystyrene solid phase with stick, which is commercially available. Testing many samples became easy with higher sensitivity.

Adult↗

PubMed1980

Dynamic properties of concentrated suspensions of charged polystyrene spheres.

Ordered structure formation of charged polystyrene spheres was studied by measuring the order-disorder phase diagrams as well as the mechanical properties. The phase diagrams indicate that the ordering of polystyrene spheres obeys Lindemann's law of crystal melting, in which the Lindemann's parameter is about 5%. The rigidity of about 10(3) dyn/cm(2) was observed in the ordered suspension of polystyrene spheres as measured by a torsional quartz crystal method. The steady flow properties of suspensions of polystyrene spheres showed a remarkable change from a Bingham body to a Newtonian liquid at the transition point. The limit of elasticity in the ordered phase was about 1 dyn/cm(2). The viscosity in the disordered phase was well explained by the free volume theory of liquids. It is concluded from these facts that the ordered phase of polystyrene spheres is a real "crystal" whereas the disordered phase is a "liquid". Properties of ordered structures in biological systems are also discussed.

Journal Article↗

PubMed2003

Real-time evidence of surface modification at polystyrene lattices by poloxamine 908 in the presence of serum: in vivo conversion of macrophage-prone nanoparticles to stealth entities by poloxamine 908.

Intravenously injected polystyrene nanoparticles, which are prone to rapid sequestration by professional phagocytes, are converted to stealth entities by prior bolus intravenous injection of poloxamine 908. This behaviour is not due to alteration in macrophage phagocytic activity. Laser Doppler anemometry and surface plasmon resonance were used to unravel the mechanisms fundamental to generation of such stealth entities in vivo by poloxamine 908. Electrophoretic mobility of poloxamine pre-coated monodisperse polystyrene nanoparticles in serum, which behave as stealth entities in vivo, was similar to that of uncoated nanoparticles incubated in poloxamine pre-treated serum. This observation supported the notion that poloxamine in serum can modify the surface of nanoparticles with similar topography to that of stealth poloxamine pre-coated particles, i.e. with polyoxyethylene chains projected from the surface. Surface plasmon resonance optical phenomenon was used for real-time monitoring of protein-poloxamine interactions and adsorption at the polystyrene interface. It was found that poloxamine can not only adsorb to a serum-modified surface but in addition poloxamine in serum can form macromolecular complexes with high affinity for adsorption to a polystyrene lattice. A role for serum albumin in surface modification of nanoparticles by poloxamine 908 is also identified. Hence, our biophysical observations correlate precisely with the in vivo longevity of uncoated polystyrene nanoparticles in poloxamine pre-treated rats. This rational and sensitive biophysical approach has unravelled the probable mechanism fundamental to generation of stealth entities in vivo and therefore has application in the design and nano-engineering of stealth colloidal carriers for optimal biological performance.

Animals↗

PubMed2000

Post-source decay in the analysis of polystyrene by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Various secondary series are observed in matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectra of polystyrene. The number and positions of the series depend on the choice of matrix and added cation. For a given treatment, series observed in linear mode are not necessarily observed in reflectron mode, and vice versa. Post-source decay analysis was used to determine that the secondary series arise at least in part from formation and decay of adducts of polystyrene with matrix species. There is some treatment-to-treatment variation, but adduct formation and decay were observed for all tested treatments. The multiplicity of secondary series makes it unclear whether post-source decay occurs for the main series (polystyrene + cation)+ ions under the conditions normally used for polystyrene analysis. Such ions do undergo post-source decay at laser fluences greater than normally used. Although only polystyrene was investigated in this work, other polymers may also produce adduct and decay series in MALDI analysis. Their presence can mask the presence of minor components in a sample, but at least as observed here, do not have a strong influence on molecular mass determinations.

Journal Article↗

PubMed2005

Influence of molecular architecture on the dewetting of thin polystyrene films.

The control of dewetting for thin polymer films is a technical challenge and of significant academic interest. We have used polystyrene nanoparticles to inhibit dewetting of high molecular weight, linear polystyrene, demonstrating that molecular architecture has a unique effect on surface properties. Neutron reflectivity measurements were used to demonstrate that the nanoparticles were uniformly distributed in the thin (ca. 40 nm) film prior to high temperature annealing, yet after annealing, they were found to separate to the solid substrate, a silanized silicon wafer. Dewetting was eliminated when the nanoparticles separated to form a monolayer or above while below this surface coverage the dewetting dynamics was severely retarded. Blending linear polystyrene of similar molecular weight to the polystyrene nanoparticle with the high molecular weight polystyrene did not eliminate dewetting.

Journal Article↗

PubMed2005

Effect of rubbing-induced polymer chain alignment on adhesion and friction of glassy polystyrene surfaces.

The friction and adhesion properties of polystyrene surfaces are studied below the glass transition temperature by means of atomic force microscopy in argon. Even at a temperature far below the glass transition, the repeated sliding of a polystyrene bead tip on the non-cross-linked polystyrene surface causes significant reduction of friction and adhesion forces. There is no measurable wear of the polystyrene surface due to repeated sliding. These decreases are associated with the alignment of the outermost polymer segments induced by repeated rubbing. There are only little changes in friction and adhesion on the cross-linked polystyrene surface in which the covalent cross-linking prevents chain realignment.

Journal Article↗

PubMed2006

Polystyrene-silica nanocomposite particles via alcoholic dispersion polymerization using a cationic azo initiator.

Submicrometer-sized polystyrene-silica nanocomposite particles have been prepared by alcoholic dispersion polymerization of styrene using commercial alcoholic silica sols of 13 or 22 nm diameter as the sole stabilizing agent. The key to the formation of colloidally stable nanocomposite particles is the selection of a cationic azo initiator (use of nonionic or anionic initiators leads either to the formation of silica-stabilized polystyrene latex particles with very low silica contents or to the precipitation of polystyrene, respectively). Neither surface modification of the silica sol nor the addition of surfactant or polymeric stabilizers is required for successful nanocomposite syntheses. The purified polystyrene-silica nanocomposite particles have relatively narrow particle size distributions, with mean diameters ranging from 331 to 464 nm as judged by disk centrifuge photosedimentometry. Thermogravimetric analyses indicated mean silica contents of 13-26 wt. %, depending on the synthesis conditions. Calcination of the polystyrene-silica nanocomposite particles leads to the formation of hollow silica shells, which indicates a well-defined core-shell morphology for the original nanocomposite particles.

Journal Article↗

PubMed2005

Control of pore hydrophilicity in ordered nanoporous polystyrene using an AB/AC block copolymer blending strategy.

Ordered nanoporous plastics with hydrophilic pore surfaces were prepared by the degradative removal of polylactide from a self-organised, multi-component composite containing two block copolymers: polystyrene-polylactide and polystyrene-polyethylene oxide. The solid-state characterization of blends containing up to 12 wt.% polyethylene oxide was consistent with nanoscopic cylinders of mixed polyethylene oxide and polylactide hexagonally packed in a polystyrene matrix. Orientation of these materials through simple channel die processing resulted in good cylinder alignment. Subsequent methanolysis/hydrolysis of the polylactide component gave nanoporous polystyrene with polyethylene oxide coated pores. The resulting nanoporous materials were able to imbibe water, in contrast to nanoporous polystyrene with no polyethylene oxide component.

Journal Article↗

PubMed1997

[Absorption spectra study for fullerene-doped in polystyrene film].

The absorption spectra of C60 and C70-doped in polystyrene film and in solution were studied. The experiment results showed that the positions of the absorption peaks for C60-doped in polystyrene film moved a little toward longer wavelength than in n-hexane, and regular solid material appeared while the ratio of C60-doped in the film increased; The absorption peaks for C70-doped in polystyrene film were almost in consistent with that in solution. We suggest that C60 molecules may be connected as "microcrystalline" in polystyrene film, while C70 may be exist as single molecule or particle less than C60 in it. The preliminary model for the growth of "microcrystslline" in polystyrene film has been proposed.

English Abstract↗

PubMed1996

In vivo uptake of lecithin-coated polystyrene beads by rat hepatocytes and sinusoidal endothelial cells.

BACKGROUND: While phagocytosis by Kupffer cells (stellate perisinusoidal macrophages) is well known and that by endothelial cells also is thought to occur under certain conditions, the uptake of large particles by hepatocytes has not been well studied. We reported previously the selective phagocytic uptake of material by hepatocytes using egg lecithin-coated silicon particles. In the present work, we describe more precisely this process following the injection of lecithin-coated polystyrene beads. Additionally, we consider the possible significance of the transcytotic action by endothelial cells. METHODS: Polystyrene latex beads (240 nm in diameter) composed of two layers of polystyrene and methyl methacrylate with a central void cavity and diameter of 140 nm were injected into male Wister-Imamichi rats. The injections were administered through the hepatic portal vein in a volume of 3 ml (concentration of the lecithin-coated or uncoated beads was 2 mg/ml). Controls received the lecithin alone at a concentration of 2 mg/ml. Liver samples were taken 5, 10, or 15 min after injection, fixed, and processed for ultrastructural analysis. RESULTS: Both lecithin-coated and noncoated beads were mainly incorporated in the Kupffer cells as well as in the endothelial cells. Bristle-coated invaginations were observed in the uptake by both cell types; however, noncoated invaginations were also active in the endothelial cells, especially on the surface facing the perisinusoidal space of Disse. Only coated beads were observed within the space or in the hepatocytes. Once taken up by the hepatocytes, the lecithin-coated beads were found either within lysosomes or in a free state in the cytoplasm. CONCLUSIONS: Uptake of 240 nm lecithin-coated polystyrene beads was observed by Kupffer cells, endothelial cells and hepatocytes. These beads were considered to be transported across the endothelial cells by transcytosis. Pseudopodia and bristle-coated invaginations were not employed by the hepatocytes when incorporating the beads.

Animals↗

PubMed2000

Fluorine ion-implanted polystyrene improves growth and viability of vascular smooth muscle cells in culture.

Vascular smooth muscle cells derived from the rat aorta were cultured on unmodified or F(+) ion-implanted polystyrene (5 x 10(12) or 5 x 10(14) ions/cm(2), energy 150 keV). In 1-day-old cultures, the cells adhered to the modified polystyrene in higher numbers and over larger contact areas. Increased resistance of the cells to trypsin-mediated detachment from the growth support indicated an improved adhesion of cells to the modified polymer at later culture intervals. The cells cultured on ion-modified polymers also were larger and had a higher total protein content. By use of immunocytochemistry, several specific protein species were increased, including the cytoskeletal alpha-actin and vimentin and the plasma membrane-associated vinculin, talin, alpha-v integrins, ICAM-1, and VCAM-1, which account for stronger cell-cell and cell-extracellular matrix adhesion. The lower number of cells found floating in the medium suggests that the spontaneous detachment of cells from the modified polystyrene was lower and that the viability of the adhered cell population was higher. As was shown by the two-parameter flow-cytometric measurements of BrdU incorporation and DNA content, as well as by (3)H-thymidine autoradiography, the cell proliferation on samples modified by the dose of 5 x 10(12) ions/cm(2) was similar to that in controls; and at the dose of 5 x 10(14) ions/cm(2), it tended to be even lower. The cells grown on the polymer implanted with the dose of 5 x 10(12) ions/cm(2) responded to a new artificially created cell-free area in a confluent cell layer by more intense migration whereas at the dose of 5 x 10(14) ions/cm(2), the migration ability of cells was similar to that on the unmodified polymer. The data revealed a higher biocompatibility of ion-implanted polystyrene with vascular smooth muscle cells in culture. There was better adhesion, differentiation, and survival, and there was neither excessive migration nor proliferation.

Animals↗