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PubMed1986

Applicability of leukocyte esterase test strip in detection of canine pyuria.

A commercially available leukocyte esterase assay was evaluated for application in analyzing canine urine for the detection of pyuria. In 229 urine samples, the leukocyte esterase activity was compared with leukocyte concentrations, as assessed by microscopic sediment analysis and chamber cell counts. The leukocyte esterase assay was specific (93.2%) for canine pyuria, but was poorly sensitive (46.0%) and did not appear to be applicable to analysis of canine urine samples.

Animals↗
PubMedDate not supplied

Sterile pyuria: consider chlamydial infection.

We report the cases of two men referred to a genitourinary medicine clinic with sterile pyuria and subsequently found to have their urethras infected with Chlamydia trachomatis. Chlamydial infection should be considered in the differential diagnosis of sterile pyuria, particularly in young, sexually active males. We discuss the consequences of inadequate treatment of the index case.

Adolescent↗
PubMed1981

[Is asymptomatic bacteriuria in pregnancy combined with pyuria? (author's transl)].

The midstream urine of 269 pregnant women between 10 and 36 weeks gestation were investigated bacteriologically by the semi-quantitative dip method and microscopically for leukocytes in the urinary sediment. In 20 of 269 urines (7%) an asymptomatic bacteriuria was found but only in 10% of these bacteriurias leukocytes were found in the urine. An isolated pyuria without significant bacteriuria was found in 15 of 249 urines (6%). The assumption that a physiological pyuria exists in pregnancy was not substantiated.

Bacteriuria↗
PubMed1963

Simplified quantitative methods for bacteriuria and pyuria.

Although pyelonephritis is a common disease, it escapes clinical detection in an undesirably high proportion of patients. The present unsatisfactory diagnostic position would be much improved by widespread screening of patients by simple yet reasonably accurate methods. Bacterial counts by the pour-plate technique and estimates of the white cell excretion per hour or day, while undoubtedly of diagnostic value, are probably unsuitable for use on a wide scale. In an attempt to find more convenient procedures a simplified stroke-plate method of bacterial counting and a simplified quantitative white cell count method were devised and applied to over 1,000 mid-stream urine samples from 398 patients. Good correlation was obtained between the simpler stroke-plate method of bacterial counting and the more time-consuming pour-plate method. The quantitative white cell procedure was a much more sensitive index of pyuria than wet-film microscopy, and comparison with the bacterial count results showed that it gave a useful indication of urinary infection. It is suggested that a quantitative bacterial count should replace non-quantitative culture methods when urinary infection is suspected and that the quantitative white cell count should be performed as a routine part of the initial clinical and laboratory assessment of all patients, followed by a bacterial count if pyuria is revealed. Experience has shown that routine urine microscopy by a precise method leads to the detection of many cases of occult urinary infection.

Journal Article↗
PubMed1991

First catch urine sediment for Chlamydia trachomatis and Neisseria gonorrhoeae culture in adolescent males with pyuria.

Traditionally, only symptomatic males or those with a history of exposure are tested for sexually transmitted diseases (STDs). Since urethral infections with Chlamydia trachomatis and Neisseria gonorrhoeae are frequently asymptomatic, a practical, sensitive, and acceptable screening method is desirable. Fifty sexually active males with pyuria (age 13 to 22 years old) diagnosed with 1+ or 2+ leukocyte esterase (LE) dipstick on first catch urine (FCU) were further evaluated by culture of urethral swabs and centrifuged FCU samples for N. gonorrhoeae and C. trachomatis. Eighty-six percent had one or more positive cultures: 17 (34%) N. gonorrhoeae, 18 (36%) C. trachomatis, and 8 (16%) both organisms. FCU culture for N. gonorrhoeae had a 100% sensitivity, specificity, and positive and negative predictive value when compared to urethral swab cultures. FCU culture for C. trachomatis had a 32% sensitivity, 95% specificity, 89% positive predictive value, and a 53% negative predictive value compared to urethral culture. On the basis of the results of this study, one urethral swab can be eliminated when evaluating male adolescents for urethritis by using spun FCU culture for N. gonorrhoeae. Continued efforts should be made to develop optimal tests to detect STDs which are reliable and encourage compliance in this high-risk group.

Adolescent↗
PubMed1971

A blue catalase screening test for pyuria and haematuria.

The blue catalase test is easy to perform and economical of materials. In a series of 529 adult female hospital patients it appeared to be a good screening test for pyuria and haematuria, detecting all but one of the specimens examined with more than 20 red and white cells combined per cubic millimetre of urine and 95.2% of those with more than 10 cells. It indicates a raised leucocyte or red cell excretion rate but is not a test for bacteriuria.

Catalase↗
PubMed1985

Leucocyte esterase-nitrite screening method for pyuria and bacteriuria.

The value of the one minute leucocyte esterase-nitrite chemical strips as a screening procedure for detecting appreciable levels of pyuria and bacteriuria was assessed by comparison with microscopy and culture results. The likelihood that a negative leucocyte esterase result indicated less than 10 white cells/cu mm by microscopy (the negative predictive value), was 90.1% and that a negative nitrite result indicated less than 10(5) organisms/ml was 91.3%. There were many false positive results with both tests, however, and the overall predictive value of a positive leucocyte esterase or nitrite test, or both, was low. The leucocyte esterase-nitrite strip was neither sufficiently sensitive nor specific enough to be used as a cost effective method for screening urines in the laboratory.

Bacteriuria↗
PubMed1993

Evaluation of Questor urine screening system for bacteriuria and pyuria.

AIMS: To evaluate the Questor automated bacteriuria and pyuria screening system; to compare its performance with that of a reference method; and to assess its usefulness in a routine clinical laboratory. METHODS: The Questor urine screening system was compared with a comprehensive regimen to detect urinary tract infection, using pour-plate viable counts to determine the numbers of bacteria present in urine samples, a wide range of other cultural methods, microscopic findings and clinical information. RESULTS: The optimal performance in detecting significant growths was a sensitivity of 93%, a specificity of 74%, a positive predictive value of 43% and a negative predictive value of 98%. The list price per test is 0.17 pounds and the capital cost of the system is 39,950 pounds. Questor can test 50 samples an hour and can be operated by one member of the laboratory staff, who is not required to make interpretative judgments--for example, a medical laboratory assistant. CONCLUSIONS: The sensitivity and specificity of the Questor was better than that obtained from other screening systems using the same protocol. The system was easy to use and is a useful addition to the methods available for screening for bacteriuria.

Bacteriuria↗
PubMed2001

Asymptomatic pyuria in diabetic women.

The aim of the present study was to determine the prevalence of and the host factors for asymptomatic pyuria (ASP) in women with type 2 diabetes. The study included 179 type 2 diabetic women and consecutive 455 non-diabetic women attending as out-patients in 1996. Patients with symptoms of a urinary tract infection were excluded. ASP was defined as the presence of more than 10 leukocytes/high-power field in a random urine sample. Diabetic women more often had ASP than non-diabetic women (27.9 vs. 15.8%, P<0.001). The prevalence of ASP was significantly increased in patients with a duration of diabetes exceeding 15 years (0 approximately 4 years; 20.3%, 5 approximately 9 years; 24.3%, 10 approximately 14 years; 23.8%, and > or =15 years; 46.3%). No differences were evident in HbA(1C) between diabetic patients without ASP and those with ASP. Diabetic women with ASP more often had diabetic retinopathy, neuropathy, nephropathy, cerebrovascular disease, ischemic heart disease, and hyperlipidemia than those without ASP. However, no statistically significant differences were evident in the prevalence of hypertension, constipation, or dementia. As the degree of neuropathy increases, it is accompanied by an increasing prevalence of ASP (none, 21.4%; blunt tendon reflexes, 24.5%; symptomatic, 50.0%; and gangrene, 66.6%). The prevalence of ASP was significantly increased in the patients with proliferative diabetic retinopathy (none, 23.2%; background, 29.4%; pre-proliferative, 18.2%; and proliferative, 50.0%). As the degree of nephropathy increases, it is accompanied by an increasing prevalence of ASP (none, 20.0%; microalbuminuria, 31.9%; macroalbuminuria, 37.0%; and renal failure, 60.0%). Thus, the prevalence of ASP is increased in women with diabetes and increased with longer duration of diabetes but was not affected by glucose control. The incidence of ASP increases significantly as diabetic microangiopathy becomes severer.

Aged↗
PubMed1990

[Two case reports of hydronephroureterosis associated with complete double pelvis-ureter accompanied by pyuria and hematuria].

We report two cases of hydronephroureterosis of the upper moiety in the complete duplex kidney disclosed by urological examination for pyuria and hematuria. The first case was in a 64-year old female and the second case was in a 68-year old female. Urinary incontinence was not seen in either case. Heminephrectomy was performed because the upper moiety was non-functioning and the upper ureter opened at the vesical neck in these cases. The postoperative course was uneventful.

Aged↗
PubMedDate not supplied

Fastidious bacteriuria and pyuria.

Mid stream specimens of urine that showed pyuria or bacteriuria on direct microscopy and no bacterial growth on initial routine culture were collected from 346 patients. These specimens were cultured for the presence of fastidious organisms using two different test systems. One system used non selective media and the other used selective media. Any fastidious organism isolated was identified by standard methods. Lactobacilli were the only fastidious organisms isolated in significant numbers. Using comparable test systems, equivalent numbers of Lactobacilli were isolated from both test and control group and because of the difficulty in specimen collection, these are to be considered as possible contaminants.

Adult↗
PubMed1981

A simple test for detecting pyuria.

Pyuria indicates inflammation within the urinary tract. A test strip, Cytur-Test, was used to detect a significant number of white cells in 100 consecutive urine samples and compared with the quantitative white cell concentration. The test was simple, rapid, easy to read, the false positive rate was low and the false negative rate was acceptable. In screening patients for urinary tract disease the Cytur-Test would be particularly useful if combined with tests for protein, blood and glucose.

False Negative Reactions↗
PubMed1978

Studies on the prevalence of renal disease and hypertension in relation to schistosomiasis. III. Proteinuria, haematuria, pyuria and bacteriuria in the rural community of Nigeria.

Midstream specimen of urine was collected from the total population of two rural communities in the immediate environs of Ibadan. One has a higher and the other a low endemicity of urinary schistosomiasis. Proteinuria, haematuria, pyuria and bacteriuria were found to be significantly more frequent in the area of high than low endemicity.

Adolescent↗
PubMed1998

Indinavir nephropathy in an AIDS patient with renal insufficiency and pyuria.

Indinavir has been described to cause crystalluria and nephrolithiasis in a variable number of treated patients. Acute renal failure, often reversible with discontinuation of the medication, induction of a diuresis and correction of urinary obstruction if present, occurs in a smaller percent of patients. One recent report described renal biopsy findings, indinavir crystals within cellular casts in the collecting tubules, in a patient receiving this antiretroviral agent. We report a second case of a patient with mild renal insufficiency and pyuria following indinavir therapy and describe similar renal biopsy findings.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

Chlamydia trachomatis detected by ligase chain reaction in the semen of asymptomatic patients without pyospermia or pyuria.

To determine whether an absence of leukocytes in semen and urine predicts an absence of Chlamydia trachomatis in asymptomatic infertile men, chlamydial DNA was detected in subjects' semen and urine by ligase chain reaction (LCR). Ninety-eight infertile men were studied, including 39 cases of oligozoospermia, 19 of azoospermia, 16 of asthenozoospermia, and 24 of normozoospermia. None of the subjects had pyospermia or pyuria. C. trachomatis was detected by LCR. Antichlamydial and antisperm antibody were also measured. C. trachomatis was detected by LCR in the semen of only 1 of 98 patients (1.02%), but not in the urine samples. In C. trachomatis-positive patients LCR, IgG, and IgA levels were higher than normal. No antisperm antibody was detected. Even if leukocytes are not observed in the semen and urine of asymptomatic infertile men, the presence of C. trachomatis in semen specimens is rarely observed. Therefore, it should be noted that the presence of C. trachomatis in such cases is addressed in the context of artificial insemination and other assisted reproductive technologies.

Antibodies, Bacterial↗