Demonstration of dye-uptake as a potential aid in early diagnosis of incipient caries.
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To estimate permeability properties of bovine dental enamel, complex impedance measurements and radioisotope diffusion experiments were carried out before and after X-ray irradiation (single dose, 72 Gy) of enamel specimens. Neither impedance measurements nor diffusion experiments showed significant changes in permeability.
A comparison was made between the rate of iodide permeation of dentin by diffusion and by filtration. Before acid etching, filtration only doubled the rate of iodide permeation relative to that obtained by diffusion. After acid etching, filtration produced a 32-fold increase in permeation. Acid etching, by removing occluding debris, facilitates filtration more than diffusion.
Iontophoretic currents (0--1.0 mA) were applied to dentin disks prepared from human third molars. A negative electrode increased 125I-permeation 10X, while a positive electrode increased 14C-lidocaine 8X. Iontophoresis appears promising as a method to increase the permeation of dentin by ionized substances of therapeutic interest.
Two independent techniques (filtration and surface tension) were developed to measure the functional tubular radii of 11 dentin and 2 glass discs. Anatomic radii were determined using SEM for comparative purposes. The functional radii of the dentin discs ranged from 5 to 40% of the anatomic radii. This differences was due to the fact that SEM visualizes only the surface while the functional techniques measure the radii within dentin tubules. It is concluded that the functional methods are preferred whenever the dynamics of fluid flow through dentin are under consideration.
The purposes of this study were: (1) to measure the effect of distance from the pulp on the hydraulic conductance of human radicular dentin; (2) to determine the influence of dentin thickness on the rates of fluid flow; and (3) to attempt to correlate dentinal tubule densities and diameters with root dentin hydraulic conductance. Dentin slabs prepared from extracted, unerupted, human third molar teeth were placed in a split-chamber device to permit quantitation of fluid filtration rate (hydraulic conductance). In the SEM portion of the study, dentinal tubule numbers and diameters were recorded. The results indicated that radicular dentin hydraulic conductance decreased with distance from the pulp and with increasing dentin thickness. Tubule density and diameter correlated well with the measured hydraulic conductances. The relatively low hydraulic conductance of outer root dentin makes it a significant barrier to fluid movement across root structure.
Analysis of these results shows that complete hard tissue bridges, aside from occurring with great frequency, produce a satisfactory protection to the pulp. In a case in which the barrier is incomplete, some future clinical problems may result as an incomplete barrier does not offer adequate protection as shown by the chronic inflammatory reaction in the remaining pulp tissue.
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The permeability of coronal dentine was investigated by measuring the hydraulic conductance of dentine discs. Reductions in dentine thickness from the enamel side of disc resulted in a greater increase in permeability than reductions from the pulpal side. Scanning electron microscopy revealed fewer dentinal tubules with smaller diameters in superficial dentine than in deep dentine. The permeability of coronal incisor bovine dentine is six to eight times less than that of unerupted coronal human third molar dentine but similar to that of human root dentine.
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Infiltration of a porous hard tissue with methyl methacrylate, followed by dissolution of the tissue provides a three-dimensional methacrylate cast of spaces within the tissue. Examination of such a cast by scanning electron microscopy provides information on the nature and extent of the pore system to a degree that cannot readily be visualized by the direct examination of fractured surfaces. The findings described concern human dentine but the technique may have a wider application in studies on the internal structure of other hard tissues.
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A simple in vitro method has been described for quantitatively measuring the rate at which isotopically labeled substances permeate through dentin in occlusal cavity preparations. The presence or absence of pulp tissue had only a minor effect on the kinetics of permeation. As the dentin was made thinner there was a resulting increase in the rate of iodide permeation. The relative rates of permeation were 3H2O greater than 131I greater than 99mTc greater than 14C-urea, a sequence that follows the molecular dimensions of these substances.
The effects on dentin permeability of burnishing NaF, kaolin, or glycerin, alone and in various combinations, were determined using an in vitro system. The results indicate that the important variable was not any of the constituents of the paste but the burnishing process itself. Burnishing dentin creates a partial smear layer on the dentin surface, thereby occluding the orifices of dentinal tubules sufficiently to reduce fluid movement across dentin 50 to 80%.
The posteruptive maturation of enamel is an important factor in caries disposition. In an experimental study the demineralization of enamel as well as its permeability and its morphological features in dependence of eruption stage were examined on extracted premolars. Chemical analysis showed variations in the Ca/P molar ratio. It is possible, that the instability of Ca-ions in crystalline configuration decreased during the time of eruption. The permeability of enamel decreased between 5 and 11.8%. It appears to be useful to concentrate preventive measure on this phase of dentition development.