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PubMed2005

[Apoptosis of human carcinoma of mouth floor KB cells and multidrug resistant KBv200 cells induced by azide methyl anthraquinone derivative].

AIM: To determine the effects of azide methyl anthraquinone derivative (AMAD) on growth inhibition and inducing apoptosis of multidrug resistant (MDR) KBv200 cells and parental drug-sensitive KB cells. METHODS: Cytotoxicity was determined by tetrazolium (MTF) assay. Reactive oxygen species (ROS) levels and mitochondrial membrane potential (deltapsi(m)) in cells were labeled with DCFH-DA and DiOC6 and tested by flow cytometry. Annexin V stain and DNA ladder were used to examine the apoptosis of KB and KBv200 cells induced by AMAD. RESULTS: AMAD was shown to inhibit the growth of KB and KBv200 cells significantly in a concentration-dependent manner, with mean IC50 of 0.36 and 0.45 micromol x L(-1), respectively. The generation of ROS increased obviously after the cells were treated with AMAD for 12 h, up to the peak in 24 h, meanwhile the levels of deltapsi(m) were time-dependently decreased. DNA fragmentation appeared on the agarose gel. Annexin V stain showed AMAD induced apoptosis of KB and KBv200 cells also in a concentration-dependent manner. CONCLUSION: AMAD showed inhibitory effect on both MDR KBv200 cells and parental drug-sensitive KB cells. The mechanism of action was associated with the increase of the cellular ROS level and the decrease of the mitochondrial membrane potential induced by AMAD, which result in cell apoptosis.

Anthraquinones↗
PubMed1990

[Determination of hemoglobin concentration using the hemoglobin azide method in traumatic emergencies].

UNLABELLED: The present study examined the clinical practicability of a new hemoglobin measurement device based on a modified azide-methemoglobin reaction (HiN3 method) in trauma patients. The accuracy of the HiN3 method was compared to the internationally well accepted hemiglobincyanide method (HiCN method) in arterial, capillary, and venous blood samples. PATIENTS AND METHODS: 1. Comparison of the methods. In 62 trauma patients, hemoglobin measurements using the HiN3 method were compared to results obtained by the HiCN method. Blood samples were taken simultaneously from the same patient to evaluate each measurement procedure in arterial (group 1), capillary (group 2), and venous blood samples (group 3). 2. Accuracy. To evaluate the accuracy of both methods we compared two venous blood samples taken simultaneously from the same patient. 3. Reproducibility. For measurement of the reproducibility of the HiN3 method, the first two results of each three-time-measurement were compared. Statistical analysis of the results was performed using regression analysis. RESULTS: 1. Comparison of the methods. Comparing the results of hemoglobin measurements resulting from the HiN3 and HiCN methods, a strong correlation was found in arterial (Fig. 2), capillary (Fig. 3), and venous blood (Fig. 4). 2. Accuracy. The HiN3 method had an accuracy of +/- 1.47% and the HiCN method +/- 2.55%. 3. The reproducibility of the HiN3 method was +/- 1.25% (Fig. 5); however, one pair of samples did show a significantly different initial value (Hb 8.4 g/dl in I, but 10.4 g/dl in I and III). DISCUSSION: From our examinations in trauma patients, we can recommend this easy to handle device, particularly for acute diagnostic purposes in emergency patients and for trend evaluations both in the hospital and in the prehospital period for rapid and precise hemoglobin determinations.

Adult↗
PubMedDate not supplied

Measurement of rate constants for quenching singlet oxygen with a Cypridina luciferin analog (2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo [1,2-a]pyrazin-3-one) and sodium azide.

The rate constants for [1O2] [MCLA] and [1O2] [NaN3] were measured by quenching the near-infrared emission (1 delta g----3 sigma g) in steady state with MCLA and NaN3, respectively. 1O2 was constantly generated by energy transfer to O2 from Ar laser-excited Rose Bengal. The Stern-Volmer plots yielded the second-order rate constants of 2.94 x 10(9) M-1 S-1 and 3.83 x 10(8) M-1 S-1 for quenching 1O2 with MCLA and NaN3 in water at pH 5.4, respectively. The 1O2 + MCLA reaction emitted light with maximum at 465 nm at pD 4.5 identical to the O(2-) + MCLA reaction.

Azides↗