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Results for “Immunity, Humoral”

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PubMed1995

Immunoparasitological studies of Trypanosoma cruzi low virulence clones from Panama: humoral immune responses and antigenic cross-reactions with Trypanosoma rangeli in experimentally infected mice.

The kinetics of humoral immune responses were investigated in mice experimentally infected with five clones of Trypanosoma cruzi isolated from different sources in Panama. Sera were collected at different timepoints post-infection. ELISA and IHA tests were used to detect antibodies against T. cruzi epimastigote antigens. The levels of T. cruzi specific antibodies increased during the course of infection; at day 90 post-infection the range was between 1:5120 and 1:10240. A high correlation was evident between ELISA and IHA results. Western blots revealed that these antibodies recognized polypeptides of 81, 76 and 71 KDa during the first weeks and 81, 76, 71, 50, 40, 28 and 12 KDa after 30-50 days. Only minor differences in antigen recognition patterns were demonstrated, suggesting that the major antigens may be represented in all clones. T. rangeli antigens were also recognized by T. cruzi seropositive sera. However, an ELISA test using antigens isolated from a genomic expression library of T. cruzi revealed that a hyperimmune rabbit serum against T. rangeli was unable to recognize the repeat sequence of SAPA (Shed Acute Phase Antigen) peptides but did recognize a number of other T. cruzi synthetic peptide antigens. The importance of these findings, in the context of Chagas' disease, is discussed.

Amino Acid Sequence↗

PubMed1981

Cellular and humoral immunity in Hodgkin's disease. I Patients in continuous long-term remission.

Cell-mediated and humoral immunity to herpes simplex virus (HSV), cytomegalovirus (CMV) and varicella zoster virus (VZV) were examined in 37 patients with Hodgkin's disease in continuous long term remission. This group had lower blast-transformation than a matched control group to all 3 antigens. Patients originally showing B-symptoms had higher transformation to VZV than those with A-symptoms. Patients treated with irradiation only had higher transformation than those treated with either chemotherapy or a combination of chemotherapy and irradiation. There was a clear tendency towards lower transformation in patients having been in remission for 2 years or less. Phytohaemagglutinin (PHA) stimulation gave lower response in the patient group than in the control group. Patients with B-symptoms had lower response than those with A-symptoms. Interferon production was specially impaired in patients with B-symptoms. The patient group had higher CF titers against HSV and CMV while the control group had higher titers against VZV. B-symptom patients had higher titers against VZV than A-symptom patients. It is concluded that HD patients have impaired immune function many years after discontinuation of therapy, but there are certain differences regarding the in vitro immunity within the patient groups.

Antibody Formation↗

PubMed1988

Characterization of humoral immune response in the serum and bile of patients with opisthorchiasis and its application in immunodiagnosis.

The humoral immune response in patients with opisthorchiasis was investigated using an enzyme-linked immunosorbent assay. IgG antibody reactive with Opisthorchis viverrini antigens was present in the serum of all patients. The infection also stimulated specific IgA and IgE antibody responses in most patients and, in practically all patients, there was a marked increase of total IgE. There was a moderate but significant correlation between serum IgG antibody level and severity of infection as judged from the quantity of eggs in the stool of the patients. There was also a significant elevation of antibody in the bile and serum of O. viverrini-infected patients who also had biliary obstruction. Analysis of paired samples from individual patients showed that while IgG was the predominant class of antibody in the serum of all patients, IgA was present at approximately the same level as IgG or higher in the bile of many patients. In addition to IgA and IgG antibodies, IgE antibody was also detectable in 50% of the bile samples. The high level of IgA antibody in the bile together with its presence in association with the secretory component suggested a selective transport and/or local production of IgA antibody by the hepatobiliary system of these patients.

Animals↗

PubMed1988

[Cellular and humoral immunity and systemic and regional fibrinolysis in patients with chronic suppurative bronchitis].

The state of cellular and humoral immunity, systemic and regional fibrinolysis was studied in 47 patients with chronic purulent bronchitis of a moderately severe and severe course. The suppression of cellular immunity, expressed in the reduction of the number and function of T-cells, the reduction of the number of theophylline-resistant cells with the unchangeable level of theophylline-sensitive lymphocytes was revealed. An additional study of O-cells made it possible to reveal an increase in the number of precursor and blocked T-cells, the prevalence of the fraction of suppressor-killer lymphocytes in T-lymphocyte subpopulation. The detected shifts lead to disturbance of T-B-cell cooperation, antibody genesis, and breakage of fibrinolysis immune regulation which determine progression of inflammatory changes in the bronchopulmonary system. Their detection and elimination form the basis of modern pathogenetic therapy of purulent forms of chronic bronchitis.

Adult↗

PubMed1983

[Humoral immunity in an idiopathic nephrotic syndrome with minimal glomerular lesions and focal and segmental hyalinosis in children].

Humoral immunity was studied in 40 children presenting with idiopathic nephrotic syndrome (INS), of which 30 with minimal supposed or proven glomerular changes (MGC) and 10 with focal and segmental hyalinosis (FSH). Such patients are capable of producing in vivo active antibodies, in response to viral or bacterial infections and after anti-poliomyelitis immunisation. They present with decreased immunoglobulins G and increased immunoglobulins M during exacerbation of the disease. These abnormalities often persist during remission as well as in patients off-therapy. IgE are often increased during periods or exacerbation as well as in remission. On the contrary, the number of B lymphocytes and their distribution according to surface Ig are normal.

Adolescent↗

PubMed1980

Natural humoral immunity in patients with malignant disease.

In order to assess the association of enhancement or suppression of humoral immune responses with cancer histology, sera from age and sex matched patients with sarcoma, melanoma and breast cancer and from normal donors were tested for natural antibodies in six serologic assays. Patients with melanoma were found to have a significantly higher level of antibody than the other groups tested. Antibody levels in patients with breast cancer were lowest.

Adult↗

PubMed1996

Humoral immunity and regulation of intrapulmonary growth of Legionella pneumophila in the immunocompetent host.

The potential role of humoral immunity in regulating intrapulmonary growth of Legionella pneumophila in the immunocompetent host was investigated using a murine model of Legionnaires' disease. Intratracheal inoculation of A/J mice with a virulent strain of L. pneumophila (10(6) bacteria per mouse) resulted in the recruitment of B lymphocytes into the lung and the development of anti-L. pneumophila Ab. Opsonization of L. pneumophila in vitro with anti-L. pneumophila-specific mAb resulted in a significant decrease in intrapulmonary growth of the bacteria at 24 to 72 h postinfection. Transmission electron microscopic analysis of lung tissue from L. pneumophila- infected mice demonstrated that while there was no significant difference between phagocytosis of the unopsonized and opsonized L. pneumophila by alveolar macrophages at 24 h postinfection, phagocytosis of opsonized bacteria by alveolar mononuclear phagocytic cells was significantly enhanced at 48 h postinfection. Depletion of A/J mice of complement before intratracheal inoculation of opsonized L. pneumophila (10(6) bacteria per mouse) did not significantly alter intrapulmonary growth of L. pneumophila. These results suggest that anti-L. pneumophila Ab, produced during replicative L. pneumophila lung infections, may regulate intrapulmonary growth of L. pneumophila in the immunocompetent host by decreasing the viability of extracellular L. pneumophila and by enhancing phagocytosis of the bacteria by alveolar mononuclear phagocytic cells by a complement-independent mechanism.

Animals↗

PubMed1987

[Cellular and humoral immune responses in calves after experimental infection with bovine herpesvirus 1].

Studied were some of the humoral and cellular factors of immunity with calves experimentally infected with Herpes virus 1. The clinical signs manifested by the infected calves were followed up for 10 to 12 days. The shedding of the virus through the nasal mucosa was demonstrated up to the eighth day, the peak values reached being between the 3rd and 5th day of infection. Demonstrated was blastic transformation in vitro with a phytohemagglutinin of the lymphocytes obtained from the peripheral blood prior to infection; the same phenomenon was observed also with each sample taken from the peripheral blood of the controls. Blastic transformation of lymphocytes was demonstrated also with the use of a specific antigen with the group infected from the 6th to the 15th day, with highest indices between the eighth and tenth day. This spoke of the presence of an immune response. The humoral immune response, followed up in its dynamics, was manifested by the presence of local antibodies in the infected calves first on the eighth day, and of serum antibodies on the 12th day. The results obtained point to the importance of both components of immunity with calves. It is of interest to note that the peak of the cell response set in on the eighth to ninth day of infection which coincided with the cease of virus shedding. On the other hand, the serum antibodies were demonstrated later--between the eighth and the twelfth day. This data showed that cellular immunity played a greater part in the recovery of calves in the case of a primary Herpes virus 1 infection.

Animals↗

PubMed1990

Measurement of the humoral immune response against Streptococcus pneumoniae type 14-derived antigens by an ELISA and ELISPOT assay based on biotin-avidin technology.

A Streptococcus pneumoniae type 14-specific ELISA and ELISPOT assay have been developed based on the use of biotinylated type 14 capsular polysaccharide (S14PS-biotin). A major advantage of this application over other methods is the use of 10-100-fold less antigen than that reported in the literature for other similar assays. Moreover, the prepared biotinylated polysaccharides are very stable and it is possible to use the same procedures for other pneumococcal polysaccharide antigens (e.g., S6BPS) with no major changes necessary in the ELISA and ELISPOT protocols. Furthermore, a simple thin layer chromatography method has been developed as a method for quality control of the biotinylated polysaccharide. Immunization with the thymus-independent antigen S14PS resulted in the induction of IgM spot-forming cells (SFC) and antibodies while S14PS-protein conjugates induced a thymus-dependent response. The immune response to the conjugates was enhanced by the addition of the adjuvant Quil A resulting in high levels of both IgG SFC and antibodies at day 14 after immunization. The developed assays are reliable and reproducible tools for studying the humoral immune response against Streptococcus pneumoniae type 14 capsular polysaccharide derived antigens.

Animals↗

PubMed2005

Potentiation of humoral immune responses to vaccine antigens by recombinant chicken IL-18 (rChIL-18).

Mammalian interleukin-18 (IL-18) is one of the pro-inflammatory cytokines involved in innate immune responses to microbial infection preceding the induction of both cellular and humoral immune responses. We assessed the potential of Escherichia coli-expressed His-tag purified recombinant chicken IL-18 (rHis-ChIL-18) as a potentiator of vaccine-induced immune responses in 3 week-old SPF chickens and compared it with several commonly used traditional immunostimulating adjuvants. We found that rHis-ChIL-18 significantly enhanced antibody responses to Clostridium perfringens alpha-toxoid and Newcastle disease virus (NDV) antigens, comparable to the Al(OH)3-gel, Miglyol and chitosan adjuvant. However, antibody responses were lower than water-in-oil (w/o) emulsions and combinations of rHis-ChIL-18 with Al(OH)3-gel, Miglyol or chitosan. Improved HI-titers for infectious bronchitis virus (IBV) were not noted. We conclude that rHis-ChIL-18, when delivered in the proper context, is a novel and safe immunostimulator in chickens.

Adjuvants, Immunologic↗

PubMed1990

Distinctive humoral immune responses following anterior chamber and intravenous administration of soluble antigen. Evidence for active suppression of IgG2-secreting B lymphocytes.

Inoculation of soluble antigen into the anterior chamber (AC) of the eyes of mice and rats induces a distinctive form of immune deviation known as anterior chamber-associated immune deviation (ACAID). Similarly, intravenous injections of soluble antigen induce immune deviation. In both instances, a selective impairment of delayed hypersensitivity (DH) is observed, whereas humoral immunity is said to be preserved. Recently, we noted that radiolabelled bovine serum albumin (BSA) was not eliminated in an immune fashion from the blood of animals pretreated with this antigen via AC and intravenous (i.v.) routes of inoculation. This was puzzling because the sera of these animals contained easily measurable anti-BSA antibodies. We have examined the characteristics of the anti-BSA humoral responses of mice following i.v. and AC inoculation of BSA in order to understand the reason for the lack of immune elimination. The results indicate that AC and i.v. recipients fail to eliminate antigen in an immune fashion because they produce insufficient amounts of complement-fixing (IgG2) antibodies, even though the other isotypes of immunoglobulins are well represented in the humoral anti-BSA response. The pattern of antibody isotype production, especially after boosting with BSA in complete Freund's adjuvant (CFA), implies that activation of IgG2-secreting, BSA-specific B cells is suppressed. Evidence is presented demonstrating this suppression to be antigen-specific and mediated by CD8+ T lymphocytes. These data are compatible with the hypothesis that interleukin-4 (IL-4)-secreting T helper (Th) cells are selectively activated in ACAID, whereas interferon-gamma (IFN-gamma)IL-2-secreting Th cells are actively suppressed.

Animals↗

PubMed2001

Cellular and humoral immune responses to Borrelia burgdorferi antigens in patients with culture-positive early Lyme disease.

We determined cellular and humoral immune responses to Borrelia burgdorferi lysate and to recombinant flagellin (FlaB), OspC, and OspA in acute- and convalescent-phase samples from 39 culture-positive patients with erythema migrans and in 20 healthy control subjects. During the acute illness, a median of 4 days after the onset of erythema migrans, 51% of the patients had proliferative cellular responses and 72% had antibody responses to at least one of the borrelial antigens tested. During convalescence, at the conclusion of antibiotic therapy, 64% of the patients had proliferative cellular reactivity and 95% had antibody reactivity with at least one of the spirochetal antigens tested. In both acute- and convalescent-phase samples, cellular immune responses were found as frequently to OspA as to OspC and FlaB. Although antibody responses were also frequently seen to OspC and FlaB, only a few patients had marginal antibody reactivity with OspA. The percentage of patients with proliferative responses was similar in those with clinical evidence of localized or disseminated infection, whereas humoral reactivity was found more often in those with disseminated disease. We conclude that cellular and humoral responses to B. burgdorferi antigens are often found among patients with early Lyme disease. In contrast with the other antigens tested, cellular but not humoral reactivity was often found with OspA.

Acute Disease↗

PubMedDate not supplied

Phenotype of lymphocytes mediating copolymer-specific humoral immunity in ethanol-consuming C57BL/6 mice.

Little is known about the mechanisms of impaired immune function in alcoholic patients. We have previously shown that ethanol consumption by mice alters copolymer-specific humoral and cellular immune responses. Does ethanol consumption eliminate suppressor T cells, allowing nonresponder mice to make humoral immune responses to poly(Glu50Tyr50) (GT)? Female C57BL/6 mice were fed a nutritionally complete liquid diet containing 35% ethanol-derived calories for up to 33 days. Control mice were fed an isocaloric control liquid diet or remained on a solid diet and water. Mice fed the ethanol-containing diet made GT-specific plaque-forming cell (PFC) responses, whereas mice fed liquid control or solid diets did not. Lymphocytes from ethanol liquid diet-consuming mice helped splenocytes from either solid or liquid control mice to make a GT-specific PFC response. The cells mediating help were nylon wool nonadherent, CD4-bearing T cells. These findings suggest that ethanol does not eliminate copolymer-specific suppressor cells, but instead alters the functional capability of helper T cells for humoral immune responses.

Alcoholism↗

PubMed1987

Sensitization through carcinogen-induced Langerhans cell-deficient skin activates specific long-lived suppressor cells for both cellular and humoral immunity.

Application of 2,4-dinitrofluorobenzene (DNFB) to BALB/c mouse skin depleted of epidermal Langerhans cells (LC) by the chemical carcinogen 7,12-dimethylbenz[a]anthracene (DMBA) activated cells which suppress both contact sensitivity and antibody production when transferred into naive host mice. Tolerance was induced by a concentration of DNFB optimal for inducing contact sensitivity in solvent-treated control mice. The cellular and humoral responses of hosts to a second antigen, 2,4,6-trinitrochlorobenzene (TNCB), were unaffected by these suppressor cells, demonstrating specificity for DNFB. Suppressor cells for cellular and humoral immunity could still be demonstrated 6 months following activation, by which time some mice had died, presumably of old age. The dose responses to sensitizer for generation of cells which suppressed contact sensitivity and antibody production differed, indicating that separate populations of suppressor cells probably inhibit these responses. Hence, during cutaneous chemical carcinogenesis, depletion of LC may allow activation of specific long-lived suppressor cells capable of inhibiting cellular or humoral antitumor immune responses.

9,10-Dimethyl-1,2-benzanthracene↗

PubMed1984

[Cellular and humoral immunity factors in salmonellosis and food toxinfections].

The present work deals with the state of cell-mediated and humoral immunity in 129 patients with salmonellosis and 185 patients with alimentary toxicoinfections, determined from the data obtained in the leukocyte migration inhibition (LMI) test and the passive hemagglutination (PHA) test with cysteine, at different stages of the disease and depending on the severity of the infectious process. The results of these investigations showed that in adult salmonellosis patients the specific reaction of T- and B-lymphocytes developed as secondary immune response. The results of the LMI test proved to be unrelated to the severity of the infectious process, while antibody formation was found to be most intensive in the acute course of the disease. The investigations also revealed that the activity of reaction in the LMI est did not depend on the presence of humoral antibodies. In the patients with alimentary toxicoinfections changes in the results of the LMI test and the PHA test with cysteine showed the same regularities as in the salmonellosis patients. This permitted the authors to suggest that diseases of Salmonella etiology prevailed in the former group.

Adolescent↗

PubMed1998

Humoral immune responses of Africans to cysteine protease-related antigens of Plasmodium falciparum.

The Plasmodium falciparum serine repeat antigen (SERA) and serine repeat protein homologue (SERPH) contain highly conserved domains that appear to encode cysteine proteases or related proteins. Humoral immune responses against the protease domains of SERA and SERPH were evaluated. Malaria-immune Africans, but not nonimmune controls, demonstrated potent humoral responses against the protease domains. As the SERA and SERPH protease domains are likely accessible to circulating antibody, these results suggest that humoral responses to the domains may contribute to antimalarial immunity.

Adolescent↗

PubMed1999

Humoral immune responses of cancer patients against "Cancer-Testis" antigen NY-ESO-1: correlation with clinical events.

Humoral immune responses against the "Cancer-Testis" (CT) antigen NY-ESO-1 are frequently observed in patients with NY-ESO-1 expressing tumors. This is in contrast to other known tumor antigens (TA) defined by antibody or cytotoxic T cell (CTL) reactivity, i.e., MAGE-1, MAGE-3, SSX2, Melan A, and tyrosinase. No NY-ESO-1 antibody has been detected in healthy controls and patients with NY-ESO-1 negative tumors. In this study, we have assessed the NY-ESO-1 serum antibody response in patients with NY-ESO-1 positive tumors of different histological types and stages using Western blotting and an ELISA. Of the 12 patients analyzed, 10 had demonstrable NY-ESO-1 antibodies at the start of the study. All patients were followed for changes in NY-ESO-1 antibody titers during the course of tumor treatment and clinical evolution. In 4 patients, an increase of NY-ESO-1 antibody titer was observed with progression of disease or extensive tumor necrosis under treatment. One patient showed a stable NY-ESO-1 antibody titer over 3 years along with gradual regression of a large tumor mass. In 5 patients, a decrease of NY-ESO-1 antibody was detected: in 1 patient after curative tumor resection, in 3 patients with partial regression of metastatic disease under chemo- and immunotherapy, and in another patient with a NY-ESO-1 negative tumor relapse. Our results indicate that the induction and maintenance of NY-ESO-1 antibody is dependent on the presence of NY-ESO-1 expressing tumors. Furthermore, changes in NY-ESO-1 antibody titers correlate with the evolution of NY-ESO-1 positive disease.

Adult↗

PubMed1988

Effect of salai guggal ex-Boswellia serrata on cellular and humoral immune responses and leucocyte migration.

Effect of alcoholic extract of salai guggal (AESG) was studied on cellular and humoral immune responses in mice and leucocyte migration in rats. Oral administration of AESG strongly inhibited the antibody production and cellular responses to sheep red blood cells in mice. It inhibited the infiltration of polymorphonuclear leucocytes and reduced the volume of pleural exudate in carrageenan induced pleurisy in rats. It showed no cytotoxic effect.

Animals↗