SEARCH · PubMed Health

Results for “In vitro fermentation”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 739 records · Page 41Linked to original sources

PubMed1993

The role of carbohydrate fermentation in colon cancer prevention.

Diet is an important factor in the development of colonic cancer. Fibre has been shown to decrease this risk. Part of this protective effect is probably mediated by colonic fermentation. About 10% of starch in the normal diet escapes digestion and absorption in the small bowel, and is therefore called resistant starch. This is a considerably larger source of fermentable substrate than fibre in the diet and could thus contribute significantly to the prevention of this malignancy. Short chain fatty acids, produced during fermentation, reduce colonic pH, affecting the intraluminal concentration of the putative co-carcinogenic secondary bile acids by precipitation, and by inhibition of their enzymatic formation from primary bile acids. The role of secondary bile acids in promoting colonic carcinogenesis is probably mediated by their cytotoxic effect on colonic mucosa, leading to a compensatory increase in proliferation. A hyper-proliferative mucosa, having an enhanced sensitivity to mutagenic substances, is associated with an increased risk of colorectal cancer. Butyrate, one of the short chain fatty acids, could be significant, as it has anti-neoplastic properties in vitro and in vivo. We conclude that fermentation is probably the key factor in the protective effect of fibre on colon carcinogenesis. Furthermore, consumption of resistant starch seems to be another way of stimulating fermentation.

Bile Acids and Salts↗

PubMedDate not supplied

Potency of biosynthetic human insulin determined in vitro.

Biosynthetic human insulin (BHI) obtained from separately synthesized A- and B-chains by recombinant DNA technology with Escherichia coli fermentation was compared with human and pork insulin of high purity in vitro. Applying four biologic tests (glucose oxidation and glucose incorporation into the lipids by rat epididymal fat pads, inhibition of lipolysis, and ATP depletion of isolated fat cells) and three receptor assays (binding competition with human fat cells, IM-9 lymphocytes, and rat liver cell plasma membranes), we could not discern significant differences of the half-maximum response by these seven methods. The only variance occurred with the ATP-depletion assay. This method disclosed 10% greater maximum reversion of isoproterenol-induced ATP depletion by BHI when compared with pork insulin.

Adenosine Triphosphate↗

PubMed2001

Dietary modulation of bacterial fermentative capacity by edible seaweeds in rats.

The effect of edible seaweeds [nori (Porphyra tenera) and wakame (Undaria pinnatifida)] on the modulation of colonic microbiota was studied in adult male Wistar rats. Each alga was fed to rats as the only source of dietary fiber and compared with cellulose. After 12 days, animals were sacrificed and cecal contents used as inoculum to ferment lactulose, citrus pectin, cellulose, nori, and wakame in vitro. Dietary treatment did not affect food intake or food efficiency, yet alga caused a significant increase in cecal weight. Nori and wakame were poorly fermented by the cellulose inoculum, with intermediate substrate degradation (76 and 57% for nori and wakame, respectively) and low metabolism to short-chain fatty acids (SCFA) (30% fermentability compared with lactulose). Cecal contents from rats fed nori and wakame showed a reduced ability to ferment all of the studied substrates compared with the cellulose inoculum, causing a reduction in SCFA production and dry matter disappearance. Only nori induced a bacterial adaptation that brought about a higher fermentation of this substrate. The different behaviors of the two algae could be due to their distinct chemical compositions. In conclusion, nondigestible components of edible seaweeds modified the metabolic activity of intestinal microflora, leading to a reduction of its fermentative capacity.

Animals↗

PubMedDate not supplied

Colonic fermentation of ispaghula, wheat bran, glucose, and albumin to short-chain fatty acids and ammonia evaluated in vitro in 50 subjects.

The production of short-chain fatty acids and ammonia was measured in 16.6% fecal homogenates from 50 subjects incubated at 37 degrees C for 6 and 24 hours. All 50 homogenates produced ammonia and short-chain fatty acids of any chain length (C2-C5). Incubation for 24 hours with dietary fiber (ispaghula husk or wheat bran), albumin, or glucose (10 mg/mL) increased the short-chain fatty acid production (43.6 +/- 2.8, 45.4 +/- 2.0, 60.3 +/- 3.2, and 65.8 +/- 3.1 mmol/L, respectively) compared with controls (21.4 +/- 1.3 mmol/L). The degradation of different substrates was associated with the production of different amounts of ammonia and short-chain fatty acids. Ispaghula, wheat bran, albumin, and glucose were fermented to acetate (> 2 mmol/L; 24-hour incubations) in 86%, 96%, 98%, and 98% of the homogenates, to propionate in 80%, 76%, 100%, and 68%, and to butyrate in 32%, 94%, 88%, and 54% of the homogenates, respectively. Isobutyrate, valerate, and isovalerate were produced from albumin in all (100%) of the homogenates, but only in 2 to 4% of the homogenates incubated with ispaghula or glucose. Ammonia was always (100%) produced after the addition of albumin and always (98%) consumed (assimilated) when glucose was fermented. Surgery (sigmoid or right- or left-sided colonic resection) did not change the pattern of ammonia and short-chain fatty acid production from these substrates. This study suggests that the different colonic flora from a large number of subjects share general biochemical characteristics, which metabolize different substrates to specific patterns of ammonia and short-chain fatty acids.

Adult↗

PubMed2002

Effect of pH and dose on the growth of gut bacteria on prebiotic carbohydrates in vitro.

The effect of pH and substrate dose on the fermentation profile of a number of commercial prebiotics was analysed in triplicate using stirred, pH and temperature controlled anaerobic batch culture fermentations, inoculated with a fresh faecal slurry from one of three healthy volunteers. Bacterial numbers were enumerated using fluorescence in situ hybridisation. The commercial prebiotics investigated were fructooligosaccharides (FOS), inulin, galactooligosaccharides (GOS), isomaltooligosaccharides (IMO) and lactulose. Two pH values were investigated, i.e. pH 6 and 6.8. Doses of 1% and 2% (w/v) were investigated, equivalent to approximately 4 and 8 g per day, respectively, in an adult diet. It was found that both pH and dose altered the bacterial composition. It was observed that FOS and inulin demonstrated the greatest bifidogenic effect at pH 6.8 and 1% (w/v) carbohydrate, whereas GOS, IMO and lactulose demonstrated their greatest bifidogenic effect at pH 6 and 2% (w/v) carbohydrate. From this we can conclude that various prebiotics demonstrate differing bifidogenic effects at different conditions in vitro.

Journal Article↗

PubMed2006

[The dynamics of microorganism populations and fermentation characters of co-cultures of rumen fungi and cellulolytic bacteria on different substrates].

In vitro co-culture technique and DGGE were used to investigate the dynamics of microorganism populations and fermentation characters of co-cultures of rumen fungi and cellulolytic bacteria at different substrates (concentrate to crude ratio in treatment A to E was all rice straw, 3:7, 5:5, 7:3 and all corn, respectively). The results showed that, compared with Oh, fungal population density at 24h increased in treatment B and C, but decreased in treatment A and D, and no fungi was detected in treatment E; but cellulolytic bacteria population at 24h increased with the increasing of concentrate to crude ratio. At the end of 48h fermentation, no fungi were detected in all treatments; cellulolytic bacteria population increased from treatment A to treatment C, but decreased from treatment D. DGGE results showed that samples collected in treatment A, B and C had similar DGGE patterns with about 11 dominant bands, but dominant bands in treatment D and E decreased markedly compared to treatments A, B and C. With the increasing of concentrate to crude ratio, pH value of the co-culture decreased dramatically (P < 0.05). During the fermentation periods, acetate was the major VFA in co-culture, the acetate to propionate ratio decreased from treatment A to treatment C, but increased from treatment D. With the increasing of concentrate to crude ratio, the total VFA at 48h increased from treatment A to treatment C, and then decreased. At the end of 48h fermentation, CMCase activity and xylanase activity were highest in treatment A. alpha-Amylase activity increased from treatment A to treatment D, but treatment E was the lowest in all treatments.

Animals↗

PubMed1976

Photodynamic action of thiopyronine on the respiration and fermentation in yeast.

The efficiency of photodynamic treatment with thiopyronine (TP) on the respiration and fermentation of yeast cells, on the metabolic oxygen uptake of isolated mitochondrial preparations from yeast, and on the enzyme activity of alcohol dehydrogenase (ADH) in vivo and in vitro were studied. Respiration was inactivated by treatment with TP and visible light more at the initial stage than was the fermentation. Isolated mitochondria and ADH in vitro were not as sensitive to treatment as were respiration and fermentation in vivo. Inactivation patterns of respiration and fermentation in vivo showed that the inactivation was not due to damage to the DNA. The hindrance of respiration and fermentation might be one of main causes of cell-death resulting from photodynamic treatment.

Alcohol Oxidoreductases↗

PubMed2002

Comparative antimicrobial spectrum and activity of the desfluoroquinolone BMS284756 (T-3811) tested against non-fermentative Gram-negative bacilli.

OBJECTIVE: To compare the in vitro activity of BMS284756, a desfluoroquinolone, with four fluoroquinolones against 129 Pseudomonas aeruginosa strains, 97 Stenotrophomonas/Burkholderia group strains and 43 Acinetobacter spp. strains by three in vitro test methods. METHODS: The activity of BMS284756 was determined using the NCCLS reference broth microdilution method and E test (AB Biodisk, Solna, Sweden). These methods were compared for test accuracy, and 5-microg disk zone diameters were compared for interpretative accuracy using the susceptible breakpoint of < or =4 mg/L recommended by the manufacturer. All strains tested were derived from the 1999-2000 SENTRY Antimicrobial Surveillance Program organism collection. RESULTS: Comparative quinolone potency against P. aeruginosa was as follows: ciprofloxacin (MIC50, 0.25 mg/L) > gemifloxacin (MIC50, 0.5 mg/L) > levofloxacin = gatifloxacin (MIC50, 1 mg/L) > BMS284756 (MIC50, 4 mg/L). The MIC50 value for BMS284756 versus Stenotrophomonas/Burkholderia group was 2 mg/L. This potency was similar to that of other quinolones reported previously. The MIC50 results for the Acinetobacter spp. were ciprofloxacin at >2 mg/L, levofloxacin, gatifloxacin and gemifloxacin at 4 mg/L, and BMS284756 at >4 mg/L, all intermediate or resistant concentrations. The E test results compared favorably with the reference dilution test results for P. aeruginosa, Stenotrophomonas/Burkholderia group, and Acinetobacter spp., with an overall essential agreement of 97.0% +/- 2 log2 dilution steps. E test MIC results tended to be slightly lower when testing Stenotrophomonas/Burkholderia group strains. The disk diffusion method correlated well for P. aeruginosa (r = 0.94), Stenotrophomonas/Burkholderia group (r = 0.84) and Acinetobacter spp. (r = 0.99) strains. CONCLUSIONS: BMS284756 was generally less active than other comparison quinolones when tested against non-fermentative Gram-negative bacilli. Its spectrum remains equivalent only if dosing schedules substantiate a proposed susceptible breakpoint of < or =4 mg/L.

Anti-Infective Agents↗

PubMed2005

Effects of specific lactic acid bacteria on the intestinal permeability to macromolecules and the inflammatory condition.

UNLABELLED: Non-live probiotic bacteria and their fermentation products can be used in milk-based formula intended for healthy infants. The effects of a milk formula fermented with Bifidobacterium breve and Streptococcus thermophilus and heated/dehydrated to inactivate the micro-organisms have been reported over the last few years to decrease the intestinal permeability to macromolecules in experimental animals in vivo and more recently to down-regulate inflammatory condition in vitro. Feeding guinea-pigs with such dehydrated fermented milk reinforced the intestinal barrier resistance to food proteins (HRP, beta-lactoglobulin). In addition, the products secreted by bacteria were capable of inhibiting the lipopolysaccharide (LPS)-induced TNF-alpha secretion by human peripheral mononuclear blood cells. The active secretion products were resistant to digestive enzymes and their anti-inflammatory properties were preserved after transepithelial transport across the filter-grown intestinal epithelial cell line, especially in inflammatory conditions. The binding of LPS to monocytes as well as NFkappaB nuclear translocation leading to pro-inflammatory cytokine transcription were inhibited by bacteria-culture supernatants. CONCLUSION: B. breve and S. thermophilus used as non-live micro-organisms in fermented infant formula seem to induce a reduction in macromolecular absorption and release metabolites exerting an anti-TNF-alpha effect, which persists after intestinal transport. Thus, specific lactic acid bacteria and their metabolites seem to affect positively the intestinal function.

Bifidobacterium↗

PubMed2005

Chemical and physical characteristics of corn silages and their effects on in vitro disappearance.

Estimating the available energy in corn silage provides a unique challenge because the silage contains variable proportions of grain and stover, each of which can differ in availability due to chemical composition and physical form. The objectives of this study were to investigate relationships among chemical components and their relationships with in vitro disappearance of ground and unground dried silages, and to quantify minimally fragmented starch in corn silage and investigate its impact and that of mean particle size (MPS) on in vitro disappearance of unground silages. Thirty-two corn silages were selected to provide diversity in dry matter, protein, fiber, and MPS. Detergent fibers were highly correlated with each other and with nonfiber carbohydrates, and were used to develop prediction equations between these constituents. Sieves with apertures > or =4.75 mm were used to isolate intact kernels and large kernel fragments, which were collected and analyzed to measure minimally fragmented starch (Starch>4.75). Dividing Starch>4.75 by total starch defined the proportion of minimally fragmented starch (Starch>4.75/Total), which ranged from 9 to 100% with a mean of 52%. Starch>4.75/Total was positively correlated with MPS (r = 0.46). The inverse of Starch>4.75/Total is an index of kernel fragmentation. Silages were prepared as whole material or ground to pass through a 4- or 1-mm screen of a cutter mill. In vitro dry matter disappearance (IVDMD) was greater for ground than for whole samples (71.7 and 61.2%, respectively). Increased IVDMD for ground samples was attributed to greater in vitro neutral detergent fiber (NDF) and neutral detergent solubles (NDS) disappearances. The IVDMD of ground samples was related to NDF and acid detergent lignin (R2 = 0.80). The IVDMD of whole corn silage was related to acid detergent lignin, Starch>4.75, MPS, and dry matter. When IVDMD was partitioned into in vitro digestible NDS (IVdNDS) and in vitro digestible NDF, the IVdNDS of whole was not uniform or completely fermented. The difference in IVdNDS between ground and whole was related to Starch>4.75/Total. In conclusion, the proportion of minimally fragmented starch provides a corn silage fragmentation index that is related to the in vitro digestion of whole silages that, if validated by in vivo trials, may be a useful quantitative substitute for the qualitative processing adjustment factor that is used currently in summative equations for estimating the total digestible nutrients of corn silages.

Carbohydrates↗

PubMed2005

In vivo antioxidant activity of methanol extract from quinoa fermented with Rhizopus oligosporus.

Previously, this author reported that the fermentation of quinoa with Rhizopus oligosporeus increased antioxidant activity, and the antioxidant activity of the 80% methanol extract of the fermented quinoa (Q-tempeh) was higher than the other extracts with n-hexane and water in vitro. In this paper, to clarify a beneficial effect of the fermentation of quinoa with R. oligosporus, the antioxidant activity of 80% methanol extract of Q-tempeh was investigated in rats ex vivo and in vivo. In the ex vivo experiment, the 80% methanol extract from Q-tempeh increased both activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the liver, and accelerated the production of 12-hydroxyeicosatetraenoic acid (12-HETE) in the lung. In rats fed vitamin E-free diets with 80% methanol extract of Q-tempeh, the alpha-tocopherol concentration, thiobarbituric acid-reactive substance (TBARS) value, and activities of GSH-Px and SOD in serum showed a similar concentration to those of the control rats fed a vitamin E-supplemented diet. However, the hepatic GSH-Px and SOD activities were higher than those in the control rats. On the other hand, in rats fed a vitamin E-free diet with the 80% methanol extract of quinoa, the serum alpha-tocopherol level was lower, and both TBARS values of serum and liver were higher than those in the control rats. From these results, the 80% methanol extract of Q-tempeh was inferred to be an active superoxide scavenger and peroxide reducer in vivo.

12-Hydroxy-5,8,10,14-eicosatetraenoic Acid↗

PubMed1996

Effects of a strain of Saccharomyces cerevisiae (Levucell SC1), a microbial additive for ruminants, on lactate metabolism in vitro.

The effect of Levucell SC, a strain of Saccharomyces cerevisiae marked as a feed additive for ruminants, was investigated in vitro on lactate metabolism by the ruminal bacteria Streptococcus bovis and Megasphaera elsdenii. The coculture between 10(7) live cells x mL(-1) of SC and a Streptococcus bovis strain in the presence of glucose reduced lactate production by the bacterial strain. Live yeast cells were able to compete with Streptococcus bovis for glucose utilization in strictly anaerobic conditions, so less glucose was available for the bacterium. SC also stimulated L-lactate utilization by a strain of M. elsdenii. The effect depended on the concentration of yeast cells added. Bacterial growth and fermentation end-product concentrations were also increased in the presence of SC. Some amino acids and vitamins, but not dicarboxylic acids, stimulated the bacterial specific activity of L-lactate uptake. SC was able to provide amino acids to M. elsdenii. In a coculture of Streptococcus bovis and M. elsdenii on glucose, the reduction of lactate concentration was improved by SC, the same trend being observed when maltose or soluble starch were used as carbon and energy source. These results indicate that SC can be a very useful tool to reduce lactate accumulation in vitro during fermentation of soluble sugars.

Acetates↗

PubMed2003

Preliminary studies on the anti-angiogenic potential of pomegranate fractions in vitro and in vivo.

We previously showed pomegranate seed oil and fermented juice polyphenols to retard oxidation and prostaglandin synthesis, to inhibit breast cancer cell proliferation and invasion, and to promote breast cancer cell apoptosis. Here we evaluated the anti-angiogenic potential of these materials in several ways. We checked a possible effect on angiogenic regulation by measuring vascular endothelial growth factor (VEGF), interleukin-4 (IL-4) and migration inhibitory factor (MIF) in the conditioned media of estrogen sensitive (MCF-7) or estrogen resistant (MDA-MB-231) human breast cancer cells, or immortalized normal human breast epithelial cells (MCF-10A), grown in the presence or absence of pomegranate seed oil (SESCO) or fermented juice polyphenols (W). VEGF was strongly downregulated in MCF-10A and MCF-7, and MIF upregulated in MDA-MB-231, overall showing significant potential for downregulation of angiogenesis by pomegranate fractions. An anti-proliferative effect on angiogenic cells was shown in human umbilical vein endothelial cell (HUVEC) and in myometrial and amniotic fluid fibroblasts, and inhibition of HUVEC tubule formation demonstrated in an in vitro model employing glass carrier beads. Finally, we showed a significant decrease in new blood vessel formation using the chicken chorioallantoic membrane (CAM) model in vivo. 'In sum, these varied studies employing different models in different laboratories overall demonstrate for the first time an anti-angiogenic potential of pomegranate fractions, suggesting further in vivo and clinical investigations (for updates: info@rimonest.com).

Angiogenesis Inhibitors↗

PubMed1989

Fermentation of carbohydrates under aerobic and anaerobic conditions by intestinal microflora from infants.

To determine how factors involved in infant carbohydrate metabolism could be effectively assayed in vitro, fecal materials from healthy infants were obtained and tested with fermentation mixtures incorporating glucose, fructose, and lactose incubated under aerobic and anaerobic conditions. The amounts of organic acids, hydrogen, carbon dioxide, and methane produced were determined. Principal component analysis indicated that products of the three sugar systems produced very similar factor patterns and, therefore, all three sugars may not be required to adequately characterize intestinal microbial fermentation processes. Moreover, the fermentation processes under aerobic and anaerobic conditions produced essentially identical cluster patterns of the factors from the three sugar systems. Collectively, the results indicated that two sugar systems, glucose and lactose, measured under aerobic fermentation conditions sufficed in accounting for most of the variation among babies.

Aerobiosis↗

PubMed2001

Effect of antibiotics, 2-bromoethanesulfonic acid and pyromellitic diimide on methanogenesis in rumen ciliate cultures in vitro.

The effects of penicillin G, streptomycin, chloramphenicol, 2-bromoethanesulfonic acid and pyromellitic diimide on total gas, methane, volatile fatty acid production and food degradability after 24 h of incubation in vitro were investigated in the cultures of two rumen ciliates. The inocula of both rumen ciliates Entodinium caudatum and Epidinium ecaudatum were used at a volume of 34 ml into the 50 ml glass syringes together with the feed and compounds tested. Despite penicillin G--streptomycin treatment methane production in both cultures was significantly decreased by the inhibitors for Epidinium ecaudatum. Methane production of the bacterial fraction of both protozoan species was significantly lower than in the whole cultures. No epifluorescence of methanogens on (or in) the cells of Entodinium caudatum was observed in contrast to Epidinium with which strong epifluorescence of methanogens on the cell surface was detected. Microscopic observation could indicate that the methane production by Entodinium caudatum was probably caused by their intracellular methanogenic activity, while methane production by Epidinium ecaudatum could be related to both the methanogenic bacterial fraction from their external surface and probably also to intracellular activity. Decreased feed degradability and differences in the fermentation end products accompanied the inhibition of methanogenesis in both in vitro cultures. Entodinium caudatum appeared to be more sensitive than Epidinium ecaudatum to the compounds tested.

Alkanesulfonic Acids↗

PubMed2001

Expression of E. coli araBAD operon encoding enzymes for metabolizing L-arabinose in Saccharomyces cerevisiae.

The Escherichia coli araBAD operon consists of three genes encoding three enzymes that convert L-arabinose to D-xylulose-5 phosphate. In this paper we report that the genes of the E. coli araBAD operon have been expressed in Saccharomyces cerevisiae using strong promoters from genes encoding S. cerevisiae glycolytic enzymes (pyruvate kinase, phosphoglucose isomerase, and phosphoglycerol kinase). The expression of these cloned genes in yeast was demonstrated by the presence of the active enzymes encoded by these cloned genes and by the presence of the corresponding mRNAs in the new host. The level of expression of L-ribulokinase (araB) and L-ribulose-5-phosphate 4-epimerase (araD) in S. cerevisiae was relatively high, with greater than 70% of the activity of the enzymes in wild type E. coli. On the other hand, the expression of L-arabinose isomerase (araA) reached only 10% of the activity of the same enzyme in wild type E. coli. Nevertheless, S. cerevisiae, bearing the cloned L-arabinose isomerase gene, converted L-arabinose to detectable levels of L-ribulose during fermentation. However, S. cerevisiae bearing all three genes (araA, araB, and araD) was not able to produce detectable amount of ethanol from L-arabinose. We speculate that factors such as pH, temperature, and competitive inhibition could reduce the activity of these enzymes to a lower level during fermentation compared to their activity measured in vitro. Thus, the ethanol produced from L-arabinose by recombinant yeast containing the expressed BAD genes is most likely totally consumed by the cell to maintain viability.

Journal Article↗

PubMed1996

Non-functional variants of yeast mitochondrial ATP synthase subunit 8 that assemble into the complex.

Subunit 8 (Y8) is a component of the proton channel of yeast (Saccharomyces cerevisiae) mitochondrial ATP synthase (mtATPase), whose function in the complex remains to be precisely defined. Y8 variants truncated at residue 46 (Lys47-->STP), or in which each of three conserved C-terminal amino acid residues (Arg37, Arg42 and Lys47) were substituted with isoleucine, have defects in assembly and function. The additional positive charge substitution (Gln29-->Lys) was introduced into each of the variants to determine whether functional compensation for these defects could be achieved. In the case of the (Lys47-->STP) variant, the additional positive charge restored the ability of cells to grow on non-fermentable substrate. By contrast, for the (Lys47-->Ile) variant the additional positive charge did not confer any improvement in cellular growth rate compared to that of cells expressing the Lys47-->Ile substitution alone. For the (Arg42-->Ile) and (Arg37-->Ile) variants, the presence of the Gln29-->Lys substitution failed to restore growth of host cells lacking endogenous subunit 8 on non-fermentable substrate. However, use of an in vitro assembly assay revealed that, unlike their respective parents (Arg42-->Ile or Arg37-->Ile), the (Gln29-->Lys Arg42-->Ile) and (Gln29-->Lys Arg37-->Ile) variants assemble into mtATPase. Thus we conclude that Arg42 and Arg37 have a role in mtATPase function, in addition to being required for assembly of Y8 into mtATPase.

Adenosine Triphosphatases↗

PubMed2004

Breast cancer chemopreventive properties of pomegranate (Punica granatum) fruit extracts in a mouse mammary organ culture.

We previously reported anticancer effects of pomegranate extracts in human breast cancer cells in vitro and also chemopreventive activity of pomegranate fermented juice polyphenols (W) in a mouse mammary organ culture (MMOC). In the present study we decided to expand the MMOC investigations to also include an evaluation of the potential chemopreventive efficacy of a purified chromatographic peak of W (Peak B), and also of whole pomegranate seed oil. In brief, an MMOC was established according to a known method. For the first 10 days of culture, the glands were treated with pomegranate fermented juice polyphenols (W), a high-performance liquid chromatographic (HPLC) peak separated from W (peak B), or pomegranate seed oil (Oil, and on day 3, exposed to the carcinogen 7,12-dimethylbenz[a]anthracene (DMBA), and for 10 days treated with the putative pomegranate chemopreventive. The glands were subsequently harvested and tumours counted by visual inspection. While W effected a 42% reduction in the number of lesions compared with control, peak B and pomegranate seed oil each effected an 87% reduction. The results highlight enhanced breast cancer preventive potential both for the purified compound peak B and for pomegranate seed oil, both greater than that previously reported for pomegranate fermented juice polyphenols.

Animals↗