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PubMed2000

Characterization of reversed-phase columns using the linear free energy relationship. III. Effect of the organic modifier and the mobile phase composition.

Retention factors determined for 31 solutes of widely different types on five columns of different chromatographic characteristics have been used to calculate the regression coefficients of the linear free energy relationship (LFER) equations. The mobile phases investigated consisted of acetonitrile-water and methanol-water, respectively, in a composition range of 20-70% (v/v) of organic modifiers. The regression coefficients of the LFER equations are characteristic of the given phase system (stationary phase, organic modifier and mobile phase composition) and represent the extent of the various molecular interactions contributing to the retention process. The effect of the characteristic of the stationary phase, the type of the organic modifier and the mobile phase composition is demonstrated and discussed. Alpha selectivity factors have been determined for various pairs of compounds. Hydrophobic or methylene selectivity can be described by the variation of the upsilon coefficient in Eq. (3) representing the difference in hydrophobicity between the stationary phase and the mobile phase. The polar or chemical selectivity of a phase system varies with the b coefficient in Eq. (3) representing the difference in acidity between the stationary phase and the mobile phase. Polar selectivity, i.e. the relative retention of polar solutes to that of a non-polar solute, e.g. toluene decreases with increasing polarity of the mobile phase. It depends also significantly on the polar characteristics of the columns. Specific selectivity, i.e. the relative retention of various polar solutes depends on the acidic or basic properties of the solutes to be separated and the chemical properties of the columns. The b regression coefficients can be used to describe the effect of mobile phase composition on the variation of specific selectivities. We have demonstrated that the LFER method provides a useful estimate of selectivity under different operating conditions by using the solvation parameters describing the different molecular interactions and the regression coefficients of the LFER equation characterizing the phase system.

Chromatography, Liquid↗

PubMed1983

Analysis of some commercial preparations for migraine treatment using ion-pair high-performance liquid chromatography with addition of salts to the mobile phase.

When appropriate salts are added to the mobile phase in ion-pair high-performance liquid chromatography (HPLC) it is possible to arrive at an isocratic solvent with which complex mixtures of nitrogenous compounds of different pKa values and lipophilic characteristics may be separated. Selectivity in the manipulation of solute retention depends on the salt type, its concentration, the percentage of organic modifier in the mobile solvent and the solute itself. In addition to a dramatic reduction in analysis time, the use of salts can also improve the resolution of closely eluted peaks. With judicious control of the pH of the mobile solvent, the addition of salts to the mobile phase can cause the retention of compounds of different pKa values to alter in a contrasting manner. Under typical ion-pair HPLC conditions, an increase in salt concentration in the mobile solvent enhances the retention of neutral compounds and reduces the retention of ionized compounds. An inverse log-log relationship between the capacity factor of a solute and the salt concentration in the mobile phase was found. Examples are given of the use of salts in mobile solvents for ion-pair HPLC of a number of pharmaceutical preparations employed for the treatment of migraine.

Analgesics↗

PubMed2002

Determination of electroosmotic flow mobility with a pressure-mediated dual-ion technique for capillary electrophoresis with conductivity detection using organic solvents.

A method is described for the indirect determination of the mobility of the electroosmotic flow (EOF), which can be carried out within a few minutes even for very low mobilities. It is independent of the direction of the EOF. It is based on the comparison of the measured mobilities of two oppositely charged reference ions (tetraphenylphosphonium and tetraphenylborate) with given mobilities in different organic solvents (methanol, acetonitrile, N,N-dimethylformamide, N,N-dimethylacetamide, propylene carbonate) at ionic strengths between 5 and 50 mM. The method is based on the sequential movement of the reference ions in a three-step process: first by a laminar flow to a certain position in the separation capillary, followed by electromigration due to application of voltage, and pressurised migration towards the detector. In this way the total mobilities of the reference ions can be determined from their residence times, and the difference to their known actual mobilities gives the mobility of the EOF. The method avoids misinterpretations caused by system- and eigen-peaks, which often bias the results especially when a conductivity detector is used. The method is suitable for all solvents, and is an advantage especially for organic and mixed aqueous-organic background electrolytes with high UV absorbance.

Electric Conductivity↗

PubMed2003

Effects of alcohol mobile-phase modifiers on the structure and chiral selectivity of amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phase.

Following a previous publication, the present paper reports additional results on the effects of alcohol mobile-phase modifiers on the structure and chiral selectivity of amylose tris(3,5-dimethylphenylcarbamate) (Chiralpak AD) chiral stationary phase (CSP). Solid-state NMR (1H/13C CPMAS) was utilized to identify and compare structural differences in Chiralpak AD caused by the various alcohol mobile-phase modifiers, many of which were not studied in the previous publication. The influences of the various alcohol modifiers (in hexane-based mobile phase) on the structure and chiral selectivity of the CSP were studied and compared. CPMAS spectra of Chiralpak AD flushed with the mobile phases displayed clear evidence of solvent incorporation into the CSP. When alcohol modifiers with varying size and bulkiness were used in the mobile phase, differences in structure and chiral selectivity were observed on Chiralpak AD based on solid-state NMR and chromatographic data. The change of t-butanol concentration in the t-butanol/hexane mobile phase caused changes of structure and chiral selectivity of the Chiralpak AD. These data further support our belief that the different chiral selectivities of the CSP associated with the use of different alcohol modifiers are due to different alterations of the steric environment of the chiral cavities in the CSP by the different mobile-phase modifiers.

Alcohols↗

PubMed1982

Limited joint mobility in diabetes mellitus of childhood: natural history and relationship to growth impairment.

We report a prospective study of the recently recognized complication of limited joint mobility in childhood diabetes, summarizing data collected over the seven years after the initial description of this sign. Of 309 patients, ranging in age from 1 to 28 years, 30% had limited joint mobility. No race or sex influence on expression of limited joint mobility was found; its appearance was temporally more influenced by age than by duration of diabetes. Of 142 patients with diabetes onset before puberty and of longer than three years' duration, 74 had limited joint mobility. Disproportionate distribution of height percentiles for age characterized this entire group, but those with limited joint mobility had four times the skewing of those without (74 vs 37% below the twenty-fifth percentile). The presence or absence of thyroid microsome or islet cell antibodies did not relate significantly to limited joint mobility. Diabetes control, assessed subjectively by clinical estimation and objectively by hemoglobin A1 levels, was generally unrelated to the joint findings. For patients with diabetes duration less than five years, there was a significant association between hemoglobin A1 and limited joint mobility, but the variability in values explained by this association was small (11%).

Adolescent↗

PubMed1993

Influence of vas deferens mobilization on rat fertility: implications regarding orchiopexy.

Children who undergo bilateral orchiopexy often have oligospermia or azoospermia in adulthood. This subfertility generally has been attributed to histological alterations in the cryptorchid testis. However, the possibility that an iatrogenic factor, such as mobilization of the vas deferens during orchiopexy, may have an adverse effect on fertility has not been studied. Six groups of mature Sprague-Dawley rats underwent right orchiectomy and the following procedures on the left side: group 1--sham operation, group 2--2 cm. of vas mobilized, group 3--4 cm. of vas mobilized, group 4--2 cm. of vas mobilized and deferential artery stripped off of vas, group 5--4 cm. of vas mobilized and deferential artery stripped off of vas and group 6--vasectomy. Each rat was then housed with 2 female rats for 24 days. Mobilization of the vas with disruption of the mesentery resulted in slight but statistically insignificant reduction in fertility. However, when the deferential artery was dissected off the vas, there was a marked reduction in fertility and fecundity. Histologically the vasa were normal without evidence of ischemic necrosis. The testes in groups 2 to 5 showed variable depletion of germ cells with shedding of germinal epithelium, and the testes and epididymides in group 5 were similar to the changes seen in vasectomized rats (group 6). No testes were necrotic. These data suggest that extensive mobilization of the vas causes subfertility in this animal model and may be secondary to a functional obstruction resulting from vas denervation. Whether a similar effect occurs during orchiopexy in humans deserves study.

Animals↗

PubMed2004

[Intra-prosthetic dislocation of the Bousquet dual mobility socket].

PURPOSE OF THE STUDY: The Bousquet system is a dual mobility head-polyethylene polyethylene-metal cup socket. The polyethylene insert retaining the femoral head moves in the noncemented metal cup, increasing both mobility and stability. MATERIAL AND METHOD: Between 1989 and 1997, seven cases of intra-prosthetic dislocation (six patients) were observed. The femoral head escaped from the polyethylene insert due to wear. On the average, this complication occurred ten Years after implantation. Risk of dislocation was high in six of the seven hips. All patients had a large sized stem screwed into the femoral neck. There was a characteristic radiological aspect with loss of the concentric head metal cup configuration. The head was applied against the upper wall of the metal cup. RESULTS: Surgical replacement was undertaken early in six patients by simply changing the insert without modifying the other stable components. Outcome remained good at three to eight Years. One patient underwent late surgery. The insert and the cup were replaced with a classical implant. Functional outcome was good but recurrent dislocation occurred. DISCUSSION: At mid-term, intra-prosthetic dislocation of dual mobility sockets appears to be exceptional. Dislocation results from polyethylene wear leading to failure of the insert to retain the prosthetic head. Wear is favored by direct phenomena (direct contact between neck and insert which can occur early if there is a small difference in the head and neck diameters) or indirect phenomena (factors limiting polyethylene metal-cup mobility). Surgical treatment is necessary. If undertaken early, replacement with a modular head and insert can be sufficient if the prosthesis has not loosened but the metal cup may have to be replaced in the event of metal-metal contact between the head and the cup. Prosthesis loosening, wear of the metal cup, or an identified cause of dislocation imply replacing the failing implants. Implantation of the dual mobility system is particularly interesting for patients with a high risk of dislocation or a chronically unstable hip prosthesis. Careful technique is required to reduce or retard the risk of intra-prosthetic dislocation. CONCLUSION: Intra-prosthetic dislocation of a dual mobility socket is an exceptional complication at mid-term. Surgical treatment is required but may be limited to simple insert replacement. Systematic use of this type of implant in young subjects must be carefully examined, but for us, the risk of dislocation does not outweigh the advantages of this original concept of dual mobility. This type of socket remains an useful preventive technique for high-risk hips or for curative treatment of recurrent dislocation.

Adult↗

PubMed2002

Physiotherapy for patients with mobility problems more than 1 year after stroke: a randomised controlled trial.

BACKGROUND: Community physiotherapy is often prescribed for stroke patients with long-term mobility problems. We aimed to assess the effectiveness of this treatment in patients who had mobility problems 1 year after stroke. METHODS: We screened 359 patients older than 50 years for a single-masked, randomised controlled trial to assess the effects of community physiotherapy. Assessments were made at baseline, 3, 6, and 9 months in 170 eligible patients assigned treatment or no intervention. The primary outcome measure was mobility measured by the Rivermead mobility index. Secondary outcome measures were gait speed, number of falls, daily activity (Barthel index scores), social activity (Frenchay activities index), hospital anxiety and depression scale, and emotional stress of carers (general health questionnaire 28). Analyses were by intention to treat. FINDINGS: Follow-up was available for 146 patients (86%). Changes in scores on the Rivermead mobility index (score range 0-15) differed significantly between treatment and control groups at 3 months (p=0.018), but only by a median of 1 point (95% CI 0-1), with an interpolated value of 0.55 (0.08-1.04). Gait speed was 2.6 m/min (0.30-4.95) higher in the treatment group at 3 months. Neither treatment effect persisted at 6-months' and 9-months' follow-up. Treatment had no effect on patients' daily activity, social activity, anxiety, depression, and number of falls, or on emotional stress of carers. INTERPRETATION: Community physiotherapy treatment for patients with mobility problems 1 year after stroke leads to significant, but clinically small, improvements in mobility and gait speed that are not sustained after treatment ends.

Accidental Falls↗

PubMed2002

Significance of electrophoretic mobility distribution to bacterial transport in granular porous media.

A method is developed to obtain the electrophoretic mobility distribution of colloidal particles by microelectrophoresis. The results demonstrate that for small particles (< 1 microm), the experimental mobility distribution must be deconvoluted to remove the effect of the random Brownian motion so that the electrophoretic mobility distribution can be obtained. For bacteria-sized particles (on the order of 1 microm or larger), the random Brownian motion is not significant, and the experimental mobility distribution represents the electrophoretic mobility distribution. The significance of the electrophoretic mobility distribution to bacterial transport is demonstrated through comparison between experimental and theoretical values of collision efficiency. Using the extended Derjaguin-Landau-Verwey-Overbeek (DLVO) theory, the electrophoretic mobility distribution of bacteria is transformed to the distribution of collision efficiencies. For strain Comamonas sp. DA001, the predicted collision efficiency values span orders of magnitude, indicating that variation of surface charge density in a monoclonal bacterial population is a cause for the orders of magnitude variation of experimentally determined collision efficiencies. However, despite the fact that the predicted and experimental alpha distributions overlap, the match is not adequate. This inadequacy is ascribed to inability to probe heterogeneity of bacterial surface hydrophobicity, and the inability of the DLVO theory to quantitatively model particle deposition.

Comamonas↗

PubMed2002

An analysis of the environmental mobility of radiostrontium from weapons testing and Chernobyl in Finnish river catchments.

The mobility of radiostrontium within the Arctic environment and surrounding area has been studied by analysing the mobility of 90Sr in river catchments that are within Finland. The environmental mobility of 90Sr deposited by both nuclear weapons testing and the Chernobyl accident has been investigated in five Finnish river catchments. Different models assessing the time-dependent mobility of 90Sr have been evaluated. No significant differences were found between the mobility of 90Sr from nuclear weapons tests and from the Chernobyl accident. Model parameters obtained by fitting to the measurements of the deposition and runoff rates of the nuclear weapons test fallout gave predictions which were consistent with the mid- and long-term contamination by the Chernobyl fallout. A comparison of 90Sr with 137Cs showed that they had similar mobility on deposition but, as time passed, the relative mobility of 90Sr increased with respect to 137Cs over a period of 5-8 years. Once the relative migration of 90Sr with respect to 137Cs reached equilibrium, its runoff rate was, on average, approximately an order of magnitude greater than 137Cs.

Arctic Regions↗

PubMed1997

An in vivo study of the intervertebral movements produced by posteroanterior mobilization.

OBJECTIVE: To measure the intervertebral movements of the lumbosacral spine when it was subjected to posteroanterior mobilization. DESIGN: An in vivo radiographic study. BACKGROUND: Posteroanterior mobilization is frequently employed in the examination and treatment of low back pain, but its mechanical and therapeutic effects are uncertain. METHODS: Lateral radiographs were taken of 12 healthy male subjects who were in prone lying. This was repeated while a static mobilization force of 150 N was applied vertically to their L4 spinous processes. The radiographic images of the vertebrae were traced and digitized. The sagittal rotation and translations of the motion segments, and the displacements of the spinous processes were then computed. RESULTS: Under the application of the mobilization loads, the lumbar motion segments were found to extend, whereas the L5/S1 segment tended to flex. It was also shown that the upper lumbar segments (L1/2-L3/4) translated posteriorly and the L5/S1 segment anteriorly. The L4/5 segment did not exhibit translation in a consistent direction. In addition, all the spinous processes were found to be displaced anteriorly. CONCLUSIONS: The movement pattern observed in this study strongly suggests that the spine is subjected to three-point bending under the application of mobilization loads. RELEVANCE: The intervertebral movements produced by posteroanterior mobilization were small in magnitude, and it is unlikely that the therapist can assess these movements manually with reasonable reliability. Mobilization should be interpreted as a passive test of the whole lumbar spine in three-point bending.

Journal Article↗

PubMed2003

Mobility limitations 1974-1991: period changes explaining improvement in the population.

The proportion of people with mobility limitations (difficulties with running, walking and stairs) decreased between 1968 and 1991 in the Swedish population aged 18-75. The distribution of predictors of late life morbidity, e.g., social class, health behaviour and childhood conditions, also changed during this period. This study explored whether the changes in these predictors over time were related to the decrease in the proportion of the population with mobility limitations. In two nationally representative interview samples (n=4468) from 1974 and 1991 of persons aged 45-71 the odds for limitations were cross-sectionally compared in ordered logistic regression models. In addition, predictors for the mobility outcome in 1974 were collected from an earlier 1968 survey and predictors for the 1991 outcome were collected from 1981. In 1974 the odds for limitations in the population was 50% higher than in 1991. Had the population composition regarding social class and housewives in 1991 been identical to 1974, the odds for limitations would have been similar in 1974 and 1991. Period improvement in social classes with poorer mobility also contributed to the overall period improvement. Health behaviours were examined as possible mediating factors. The increase of physically active people between 1968 and 1974 was related to the period improvement in mobility between 1974 and 1991. Smoking showed an increased association with mobility limitations during the period, indicating that mobility improvement would have been greater if everyone had been a non-smoker. Results indicate how sensitive disability rates may be for cohort or period effects.

Adolescent↗

PubMed1982

Human albumin variants. Nomenclature of allotypes observed in Europe and quantitative estimation of their relative mobilities.

In order to clarify the actual nomenclature of the albumin allotypes in French and Italian populations we compared the samples collected in our laboratory to those kindly supplied by Dr. Porta (Italy) with reference to albumin variants classified in starch gel electrophoresis using three buffer systems by Weitkamp. The inherited human albumin variants can be classified on the basis of their relative mobilities on cellulose acetate electrophoresis compatively to human transferrin mobility. The relative mobility of each variant can be expressed by the following ratio: migration distance of the variant versus migration distance of the normal albumin where zero represents the transferrin mobility. Using three buffers system at pH 8.6, 5.0 and 6.9, it is possible to distinguish some albumin variants having a same mobility at alkaline pH and different mobilities at acidic pH. In the european area, eleven albumin variants are distinguishable on the basis of their relative mobilities at different pH: four Fast moving variants: Gent, Vanves (a new variant described here), Reading and CN/BL, and seven Slow moving variants: MI/MI Slow, GE/CT, SO/BS (or D type), Pollibauer, Gainesville, Roma and B type. Thirty-six sera from unrelated subjects with genetic bisalbuminemia were analyzed in our laboratory. Their distribution was as follows: B type (22), Pollibauer (9), SO/BS (2), Gainesville (1), Gent (1) and Vanves type (1). The frequency of bisalbuminemia was 0.35 per 1,000 in a population of 19,949 blood donors.

Blood Protein Electrophoresis↗

PubMed2002

Achilles tendon healing: long-term biomechanical effects of postoperative mobilization and immobilization in a new mouse model.

The aim of the study was to investigate the long-term effects of postoperative immobilization as opposed to mobilization on the biomechanical attributes of healing Achilles tendons in a new experimental mouse model. In 114 Balb-C-mice the left Achilles tendon was transected and sutured by the Kirchmayr-Kessler technique. The tendons healed either under postoperative immobilization effected by fixing the upper ankle joint in equinus position or under mobilization through a limited range of movement. The contralateral Achilles tendons served as internal control. All tendons were tested biomechanically at short intervals up to the 112th postoperative day in terms of load to failure [N], tendon deflection [mm] and tendon stiffness [N/mm], and were evaluated histologically after 8 and 112 days. Postoperative mobilization resulted in a continuous and significantly more rapid restoration of load to failure in comparison to the immobilization group. Tendon deflection was decreased by postoperative mobilization, whereas under immobilization it paradoxically increased still further in the later course. After 112 days the tendons of the mobilization group had regained their original tendon stiffness, whereas the tendons after immobilization reached only about half the values seen in the control tendons. Histologically, postoperative mobilization led to increased immigration of inflammatory cells in the early phase. In the late phase, as compared to immobilization, tendon structure was more mature, with fibre bundles arranged in parallel and interposed tendocytes. Tensile loading of the healing tendon by postoperative mobilization leads to fundamental changes in the biological process of tendon healing resulting in accelerated restoration of load to failure and reduced tendon deflection.

Achilles Tendon↗

PubMed1997

Late improvements in mobility after acquired brain injuries in children.

The purpose of this investigation was to describe the late improvements in functional mobility in children who have sustained severe acquired anoxic or traumatic brain injuries. Ninety-eight children from a consecutive series of 199 with acquired brain injuries met inclusion criteria. As expected, children with traumatic injuries had better mobility at time of discharge from rehabilitation than did children with anoxic injury. In addition, children with traumatic injuries improved more in mobility status during the first 2 years after injury than did children with anoxic injury. The children who continued to improve in mobility after discharge were unconscious for a shorter time. Children who became community ambulators during the first year after discharge had higher mobility ratings at discharge. Although children who eventually achieved more functional mobility were admitted to rehabilitation service sooner after injury, discriminant analysis revealed that the duration of unconsciousness, and therefore severity of injury, was more closely correlated with the final mobility status.

Adolescent↗

PubMed1990

Orientation effects on the electrophoretic mobility of rod-shaped molecules in free solution.

The effect of molecular orientation on the electrophoretic mobility of rod-shaped polylons as measured by free solution capillary electrophoresis is studied by using the tobacco mosaic virus (TMV) as a model solute. This orientational dependence of molecular mobility is measured by observing the influence of electrical field strength (up to 400 V/cm) on the electrophoretic mobility of TMV. The electrophoretic mobility of TMV increases with increasing field strength. This increase can be quantitatively correlated with the decrease in the translational frictional coefficient (f) due to the increasing alignment of TMV with the electric field. A model is developed relating the decrease in f to the alignment of TMV with the electric field through its polarizability and aspect ratio. To confirm the observed orientational affects on mobility, control experiments were performed with 0.364 micron diameter Latex spheres. Due to their spherical symmetry, no orientational effects would be expected. Indeed, no increase in mobility was observed for these spherical particles. Calculations are presented to demonstrate that the increase in mobility is unlikely to be caused by either the Wien effect or any temperature variation resulting from Joule heating of the electrophoresis buffer.

Chemical Phenomena↗

PubMed2004

Simulation studies on the effects of mobile-phase modification on partitioning in liquid chromatography.

Various driving forces have been suggested to explain retention and selectivity in reversed-phase liquid chromatography (RPLC). To provide molecular-level information on the retention mechanism in RPLC, configurational-bias Monte Carlo simulations in the Gibbs ensemble were carried out for model systems consisting of three phases: an n-hexadecane retentive phase, a mobile phase with varying water-methanol composition, and a helium vapor phase as reference state. Liquid n-hexadecane functions as a model of a hydrophobic stationary phase, and a wealth of experimental data exists for this system. Gibbs free energies for solute transfers from gas to retentive phase, from gas to mobile phase, and from mobile to retentive phase were determined for a series of short linear alkanes and primary alcohols. Although the magnitude of the incremental Gibbs free energy of transfer for a methylene segment is always larger for the gas- to retentive-phase transfer than the gas- to mobile-phase transfer, it is found that the partitioning of alkanes and alkyl tail groups is mostly affected by the changes in the aqueous mobile phase that occur when methanol modifiers are added. In contrast, the partitioning of the alcohol headgroup is sensitive to changes in both the n-hexadecane and the mobile phases. In particular, it is found that hydrogen-bonded aggregates of methanol are present in the n-hexadecane phase for higher methanol concentrations in the mobile phase. These aggregates strongly increase alcohol partitioning into the retentive phase. The simulation data clearly demonstrate that due to modification of the retentive-phase hydrocarbons by solvent components, neither the solvophobic theory of RPLC, advocated by Horvath and co-workers, nor the lipophilic theory of RPLC, advocated by Carr and co-workers, can adequately describe the separation mechanism of the hexadecane model system of a retentive phase studied here nor the more complex situation present in actual RPLC systems.

Journal Article↗

PubMed2005

Mobility moment analysis of molecular interactions by capillary electrophoresis.

A new method for the quantitative evaluation of molecular interactions that are observed in electrophoresis is described. One component taking part in the interaction is labeled with a fluorescent dye and is subjected to capillary zone electrophoresis with fluorescence detection in the presence or absence of an unlabeled interacting component. Fluorescence signals are collected at constant time intervals, and the electropherograms are converted to represent the fluorescence signal against mobility. After baseline subtraction, the first statistical moment of fluorescence signals on the mobility axis is calculated. This moment represents the average mobility of a labeled component. The change in the mobility moment in the presence and absence of the unlabeled component is used to evaluate the degree of saturation of the binding site of a labeled molecule with an unlabeled molecule. Mixtures of fluorescence-labeled protein (Fab' fragment of antibody or concanavalin A) and its unlabeled interacting partner (alpha(1)-antitrypsin or succinylated ovalbumin, respectively) at various concentrations were injected into a bare-silica capillary, and zone electrophoresis was carried out. The change in the mobility moment of the fluorescence-labeled molecules was used to determine the dissociation constants of the complexes. The determined constants are comparable to those obtained by a well-established method, that is, an analysis based on the peak height of the complex. Since the mobility moment analysis is not affected by the total intensity of the signals, it should be advantageous in analyses in which multiple capillaries are used, in which the injection volume and the sensitivity of detection might be more difficult to control at constant values. The mobility moment analysis also has advantages for the analysis of heterogeneous samples, since the identification of peaks is not necessarily required.

Antigen-Antibody Reactions↗