PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “Phase Separation”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 739 records · Page 41Linked to original sources

Magnetisable solid-phase separation fluoroimmunoassay for total oestriol in pregnancy serum.

A rapid separation fluoroimmunoassay for serum or plasma levels of total oestriol in pregnancy was established, based on the use of fluorescein-labelled oestriol and sheep anti-oestriol serum covalently linked to magnetisable particles. Equilibrium was attained within 10 min, and the fluorescence of the bound fraction of labelled ligand was quantitated fluorimetrically after elution from the magnetisable particles. Results for pregnancy serum samples correlated well with an established radioimmunoassay technique and the sensitivity, precision and accuracy were appropriate for clinical use. Advantages of this system as compared with radioimmunoassay include the speed and simplicity of end-point detection, prolonged shelf-life of the labelled reactant and absence of any health hazard. The separation step enabled the removal of any endogenous fluorophores or other interfering factors present in biological samples.

Animals↗

Phase separation, charge separation and biogenesis.

The current view of bioenergetics postulates transmembrane charge separation as a primary mechanism of energy storage and transformation. Using that bioenergetic view we examine possible methods of photon driven transduction in primordial vesicles. Two possible types of proton pumps are analyzed and a method of anaerobic photophosphorylation is discussed. Using these principles we theorize about the formation of prebiotic photochemical vesicles utilizing the same transmembrane energy conversions characteristic of contemporary cellular systems.

Anaerobiosis↗