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At least 739 records · Page 41Linked to original sources
PubMed1991

Calpain activity in a subcellular fraction enriched in partially degraded CNS myelin fragments compared with myelin.

Marchi-positive bodies are structures present paranodally in large myelinated nerve fibers. They have morphological and biochemical characteristics closely resembling the partially degraded myelin fragments formed during the early phases of Wallerian degeneration. Levels of calcium-activated neutral proteases (calpains) and their endogenous specific inhibitor calpastatin were measured in highly purified rabbit myelin and a spinal cord subcellular light ('floating') fraction heavily enriched in Marchi-positive bodies. Calpain levels were found to be significantly higher in the floating fraction as compared to myelin. No calpastatin was detectable in either fraction.

Animals↗
PubMed1985

Distribution of gentamicin among subcellular fractions from rat renal cortex.

A substantial amount of data is available to suggest that lysosomal sequestration of aminoglycoside antibiotics plays a role in the pathogenesis of aminoglycoside-induced renal tubule cell injury; however, relatively little information is available on the subcellular distribution of aminoglycosides in the kidney during treatment protocols of the type that ultimately go on to produce extensive lethal renal tubule cell injury and acute renal failure in experimental animals. This study assessed the distribution of gentamicin and subcellular membranes on a discontinuous sucrose density gradient after in vivo exposure of rats to four daily 100 mg/kg doses of gentamicin as compared to in vitro exposure of normal rat renal cortex to gentamicin during tissue homogenization at drug levels comparable to those seen after in vivo treatment. After both in vivo and in vitro exposure, major localization of gentamicin, the lysosomal marker enzyme N-acetyl-beta-D-glucosaminidase (NAG), and the endoplasmic reticulum marker enzyme NADPH-cytochrome c reductase, occurred in a very light membrane fraction. Within this membrane fraction, gentamicin was more closely associated with the NAG than with the NADPH-cytochrome c reductase. The results could not be explained by complete lysosomal disruption during subcellular fractionation after in vivo gentamicin. These data provide additional insights into both the possibilities for subcellular interactions of aminoglycosides in the kidney, and into the methodology required to optimally assess such interactions.

Acetylglucosaminidase↗
PubMed1998

Inhibition of [3H]quinpirole binding by a monoamine oxidase inhibitor in subcellular fractions of rat striatum.

[3H]Quinpirole is a dopamine agonist with high affinity for D2-like dopamine receptors. A number of non-dopaminergic compounds, most notably monoamine oxidase inhibitors (MAOIs), inhibit the binding of [3H]quinpirole, but not other D2-like agonists and antagonists, in rat striatal membranes by a mechanism that does not involve the enzymatic activity of MAO. To further characterize this novel interaction, the subcellular distribution of spiperone-displaceable, "D2-like" [3H]quinpirole-labeled sites in rat striatum was assessed and compared with the distribution of MAOI-displaceable [3H]quinpirole binding (MQB). "D2-like" [3H]quinpirole binding exhibited similar nanomolar affinity in the crude synaptosomal (P2), crude microsomal (P3), and ribosomal, post-microsomal (P4) fractions. Total binding activity (fmol bound/fraction) of "D2-like" [3H]quinpirole binding was concentrated in the synaptosomal fraction (P2B). The subcellular distribution of MQB paralleled that of "D2-like" [3H]quinpirole binding. This suggests that "D2-like" [3H]quinpirole binding and MQB occur at a common membrane-bound binding site.

Animals↗
PubMed1983

Phosphatidic acid phosphatase activity in subcellular fractions derived from adult rat type II pneumocytes in primary culture.

The development of a method for the subcellular isolation of lamellar body, microsomal, mitochondrial and cytosolic enriched fractions from the adult rat type II pneumocyte is presented. These fractions were used to investigate the distribution and specificity of phosphatidic acid phosphatase (EC 3.1.3.4). The cytosolic enriched fraction possessed the greatest proportion of total phosphatidic acid phosphatase activity and the highest relative specific activity. The small amounts of phosphatase activity in the lamellar body, microsomal and mitochondrial enriched fractions correlated best with a nonspecific phosphatase activity. 1,2-Disaturated phosphatidic acid served as a slightly better substrate than did 1-saturated, 2-unsaturated phosphatidic acid in the cytosolic enriched fraction. This observation indicates that the de novo pathway could contribute to dipalmitoylphosphatidylcholine synthesis in the rat type II pneumocyte.

Animals↗