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PubMed2002

Occurrence of trans-C18:1 fatty acid isomers in goat milk: effect of two dietary regimens.

Trans-octadecenoic acid composition of goat milk fat was studied by using silver-ion thin layer chromatography combined with gas-liquid chromatography. This analytical procedure also was used to investigate the effect of diet on trans-C18:1 fatty acid content in goat milk. Thirty-two goats were used in a 2 x 2 factorial arrangement and treatments. Two groups of goats received alfalfa hay at either a high or a low level of forage and two other groups received Rumiluz (dehydrated alfalfa from France Luzerne, Châlon en Champagne, France) at either a high or a low level of forage. Trans-C18:1 isomer proportions (relative to total fatty acids) were, respectively, 2.02% for the Rumiluz low-level group and 1.75% for the Rumiluz high-level group versus 1.71% for the alfalfa low-level group and 1.21% for the alfalfa high-level group. Goats fed on Rumiluz thus produced relatively higher levels of trans-C18:1 fatty acids than animals fed alfalfa hay. The results also showed that production of trans-C18:1 fatty acids increased when the level of forage in the diet decreased. Moreover, goat milk trans-C18:1 composition appeared similar to the cow milk profile. Vaccenic acid, trans-11-C18:1, was the major component and represented about 36.2% of total trans-C18:1 isomers.

Animal Feed↗

PubMed2003

Study of nutritive utilization of protein and magnesium in rats with resection of the distal small intestine. Beneficial effect of goat milk.

The search for diets to improve the nutritive utilization of protein and magnesium in malabsorption syndrome led us to study goat milk, because of its particular nutritional characteristics, and to compare it with cow milk, which is most commonly consumed. We studied the nutritive utilization of protein and magnesium in transected rats (control) and in rats with resection of 50% of the distal small intestine. The diets used were the standard diet recommended by the American Institute of Nutrition and diets based on lyophilized goat or cow milk. The consumption of goat milk produces better protein efficiency ratio and food conversion efficiency values, particularly in rats with intestinal resection, together with a higher nutritive utilization of protein. Magnesium apparent digestibility coefficient is not modified by intestinal resection in rats fed with goat milk-based diet, on the contrary to the standard and cow milk diets. Magnesium apparent digestibility coefficient is greater for the goat milk group, which is reflected in the greater quantity of this mineral stored in bone. These results demonstrate the beneficial effect of goat milk on the nutritive utilization of protein and on magnesium bioavailability, especially in animals with resection of the distal small intestine.

Animal Nutritional Physiological Phenomena↗

PubMed2006

Factors affecting growth factor activity in goat milk.

Growth factors that are present in goat milk may be responsible for its beneficial effects on the digestive system as described in ancient Chinese medical texts. To develop a nutraceutical product rich in growth factors for promoting gastrointestinal health, it is essential to collect milk with consistently high growth factor activity. Therefore, we investigated the factors affecting growth factor activity in goat milk. Among the 5 breeds of dairy goats tested, milk from Nubian goats had the highest growth factor activity. Tight-junction leakage induced by a 24-h milking interval did not increase growth factor activity in the milk. Milk collected from pregnant does had a significantly higher growth factor activity than milk collected postpartum. Growth factor activity decreased during the first 8 wk of lactation, fluctuated thereafter, and then increased dramatically after natural mating. During wk 1 to 8, growth factor activity was inversely correlated with milk yield and week of lactation. No correlation was observed during wk 9 to 29. After natural mating of the goats, the growth factor activity in the milk correlated significantly with somatic cell count and conductivity (a measure of membrane permeability), and correlated inversely with milk yield. Based on the above data, goat milk with higher growth factor activity could be selectively collected from Nubian pregnant does.

Animals↗

PubMed1976

Blood and liver metabolites in fed and fasted diabetic goats.

Two trials were to study alloxan diabetes in goats. The data were grouped: 1) normal fed goats (10); 2) 48-h fasted goats (5); 3) fed goats sampled 96 h after alloxan treatment (5); and 4) goats treated with alloxan following a 48-h fast and sampled 96 h after alloxan treatment with continued fasting (3). Groups 1 and 4 exhibited the following means: serum insulin 43.9, 16.4, 9.4, and 6.7 muU/ml; blood glucose 55.0, 47.3, 219.6, and 485.6 mg/100 ml; blood ketones 4.3, 2.6, 36.6, and 28.6 mg/100 ml; blood acetate 4.7, 4.0, 42.7, and 4.9 mg/100 ml; plasma-free fatty acids 1.8, 10.0, 14.4, and 40.5 mg/100 ml; and plasma triglyceride 13.3, 7.0, 47.6, and 12.2 mg/100 ml. Liver samples from five fed goats before and 12 days after alloxan treatment exhibited the following means: phospholipid 27.5 and 26.1 mg/g; triglyceride 21.2 and 98.9 mg/g; and percent lipid 7.2 and 14.4. The diabetes was accompanied by fatty liver development and probably reduction in utilization of acetate and triglyceride in the fed animals.

Animals↗

PubMed1986

Bioavailability of iron in goat milk compared with cow milk fed to anemic rats.

Bioavailabilities of iron from dehydrated whole and skim goat milk were investigated using iron-deficient rats. Hemoglobin regeneration efficiencies were determined as the percent conversion of dietary iron into hemoglobin. The respective hemoglobin regeneration efficiencies for groups fed whole goat milk, whole cow milk, skim goat milk, and skim cow milk were 50.6, 13.1, 26.0, and 13.0%, indicating that iron bioavailability of goat milk was greater than cow milk. However, rats fed each milk had negative net increases in hemoglobin concentrations, implying that the iron contents of each milk were not adequate. For animals consuming whole goat milk supplemented with ferrous sulfate, the slope relating hemoglobin iron gained versus iron intake was .95. Respective bioavailabilities relative to ferrous sulfate were 54, 14, 28, and 14% for the four sources of milk. Iron bioavailability of goat milk is superior to cow milk when fed to anemic rats.

Anemia, Hypochromic↗

PubMed1989

Metabolic responses of lactating goats to feed restriction and dietary 1,3-butanediol.

Feed restriction and dietary 1,3-butanediol were used with lactating goats in an attempt to induce metabolic changes characteristic of bovine lactation ketosis and fatty liver. In Experiment 1, midlactation goats were fed 80, 102, or 114% of metabolizable energy requirements and 0, 50, or 100 g/d of 1,3-butanediol. Concentration of beta-hydroxybutyrate in blood plasma decreased with increasing metabolizable energy but was increased greatly at 2 h after goats were fed 50 or 100 g butanediol and remained elevated at 6 h postfeeding with 100 g of butanediol. Concentration of glucose in plasma was decreased at 2 and 6 h postfeeding in goats fed 100 g of butanediol. In Experiment 2, goats in early lactation were fed for ad libitum intake or were restricted to 70% of ad libitum intake with 1,3-butanediol included at 10% of diet DM. The treatment decreased milk production, increased concentrations of beta-hydroxybutyrate and nonesterified fatty acids, and decreased the concentration of insulin and the insulin to glucagon ratio in plasma. Concentrations of glucose, acetate, and glucagon in plasma were not affected. After 28 d of treatment, concentration of total lipid in liver was increased, but concentrations of glycogen and triglyceride were unaffected. Changes caused in goats by feed restriction plus dietary 1,3-butanediol were characteristic of subclinical lactation ketosis in cows, but the response was more moderate than seen previously in cows.

3-Hydroxybutyric Acid↗

PubMed1991

Influence of breed, animal, and days of lactation on lactoperoxidase system components in goat milk.

Lactoperoxidase and thiocyanate content in goat milk from two Spanish breeds throughout 5 mo of lactation has been investigated. Mean lactoperoxidase for milk from Verata and Murciano-Granadina goats was .95 and 2.15 units/ml, respectively. Very low concentrations, .03 units/ml for Verata and .20 units/ml for Murciano-Granadina, were found for the first 24 h after kidding. Highest concentrations were detected at the end of lactation (135 to 150 d) for Verata and in midlactation (60 to 75 d) for Murciano-Granadina goats. Mean thiocyanate content was 5.76 ppm for Verata goats, without a distinct maximum throughout lactation, and 3.20 ppm for Murciano-Granadina goats, with maximum levels at final lactation stages. Activation of the lactoperoxidase system might be a useful procedure in preserving raw goat milk quality by addition of low levels of thiocyanate and hydrogen peroxide.

Animals↗

PubMed1997

Influence of kid rearing systems on milk composition and yield of Murciano-Granadina dairy goats.

One-hundred eight lactations of Murciano-Granadina goats from different years were used to compare two kid rearing systems. Goats were separated into two groups: suckling and milking. Dams in the suckling group were milked once daily until kids were weaned (wk 0 to 7) and then were milked twice daily. Dams in the milking group were separated from their kids at 48 h after birth; then, kids were raised artificially, and goats were milked twice daily. Total milk yield was estimated according to the oxytocin method during suckling. Stage of lactation, parity, prolificacy, and year effects on milk yield and composition were also studied. As expected, during the first 7 wk of lactation, marketable milk was higher for dams that were milked than for dams that were suckled. Neither milk yield nor milk composition throughout the entire lactation was affected by group or prolificacy with the exception of the percentage of milk CP. The lactation curve peaked at wk 4 or 5 and declined slowly afterward. First parity goats had the lowest milk yield but the highest fat and protein percentages. Third parity goats had the highest milk yield. The separation of kids from their dams after birth did not affect total lactation performance because of the minimal importance of the neuroendocrine milk ejection reflex in goats compared with that of other ruminants.

Animals↗

PubMed1999

Influence of feed intake and source of dietary carbohydrate on the metabolic response to propionate and glucose challenges in lactating goats.

The influence of type of nutrients in the diet (rapidly degraded starch plus rapidly degraded N or highly digestible fiber plus slowly degraded N) and amount of feed offered (2.40 +/- 0.10 or 2.00 +/- 0.10 kg of dry matter/d) on plasma constituents after a glucose tolerance test or a propionate challenge test were studied using 12 multiparous dairy goats. Type of diet and interaction between type of diet and amount of feed offered had no effect on the concentrations of any plasma metabolite measured during propionate challenge. During propionate challenge, goats fed at the high dry matter intake (DMI) had a higher clearance of propionate and glucose and a higher ratio of plasma insulin to glucose. During glucose challenges, goats fed diets that were rich in highly digestible fiber tended to have lower peak concentrations of glucose, and goats fed at the high DMI had a higher clearance of glucose. The plasma insulin response to the glucose test was not altered by treatment. The ratio of plasma insulin to glucose was numerically, although nonsignificantly, higher for goats fed at the high rate of DMI. Most of the differences concerning glucose metabolism, insulin response, and tissue responsiveness to insulin appeared to be primarily due to the amount of feed offered and not to the type of diets fed to dairy goats in midlactation.

3-Hydroxybutyric Acid↗

PubMed1998

Validation of antibiotic residue tests for dairy goats.

The SNAP test, LacTek test (B-L and CEF), Charm Bacillus sterothermophilus var. calidolactis disk assay (BsDA), and Charm II Tablet Beta-lactam sequential test were validated using antibiotic-fortified and -incurred goat milk following the protocol for test kit validations of the U.S. Food and Drug Administration Center for Veterinary Medicine. SNAP, Charm BsDA, and Charm II Tablet Sequential tests were sensitive and reliable in detecting antibiotic residues in goat milk. All three assays showed greater than 90% sensitivity and specificity at tolerance and detection levels. However, caution should be taken in interpreting test results at detection levels. Because of the high sensitivity of these three tests, false-violative results could be obtained in goat milk containing antibiotic residues below the tolerance level. Goat milk testing positive by these tests must be confirmed using a more sophisticated methodology, such as high-performance liquid chromatography, before the milk is condemned. LacTek B-L test did not detect several antibiotics, including penicillin G, in goat milk at tolerance levels. However, LacTek CEF was excellent in detecting ceftiofur residue in goat milk.

Animals↗

PubMed1985

Goat's milk and infant feeding.

As goat's milk becomes more popular in Australia, clinicians are being asked about its suitability as an infant feed. The popularity of goat's milk in part comes from unsubstantiated claims that it is less allergenic and more digestible than is cow's milk. Raw goat's milk is used by those who believe that pasteurization is detrimental, although the risks of consuming unpasteurized milk have been well documented and recent reports have warned about the dangers of feeding unmodified goat's milk to infants. If goat's milk is to be fed to infants it should be pasteurized or boiled, diluted to reduce solute load, and then fortified with vitamins. Although these modifications do not produce an ideal milk, they do minimize the risks of using goat's milk as an infant feed.

Animals↗

PubMed1999

Cardiovascular changes associated with dehydration and drinking in unrestrained, lactating goats.

The aim of this study was to investigate if the alertness connected with seeing water increased arterial blood pressure and heart rate to the same extent as the act of drinking, and if ingestion of warm water caused a different effect compared with ingestion of cool water on these cardiovascular variables. Seven goats of the Swedish domestic breed (Capra hircus) were used in a cross-over design. The animals were dehydrated for 24 h. They were allowed to watch water being prepared for 11-16 min, after which they were given access to warm (35 degrees C) or cool (15 degrees C) water. The goats drank 6.86 +/- 0.36 l of the warm water and 4.54 +/- 0.35 l of the cool water (P < 0.05) within the first hour. The arterial blood pressure, heart rate and activity of the animals were registered by an implanted telemetric device. Dehydration did not affect the cardiovascular variables, except before feeding in the morning, when the heart rate accelerated faster in dehydrated goats. Heart rate increased abruptly when dehydrated goats saw water being prepared, remained at the increased level during drinking and then slowly declined. It increased again during the afternoon feeding, to a level similar to that on control days, but between 18.00 and 06.00 h the heart rate was higher than during control nights. Blood pressure did not change when the goats saw water, but increased when they drank. On the morning following rehydration, the rise in heart rate in response to feeding was delayed compared with that during control and dehydration periods. It is concluded that seeing water caused arousal in the goats, resulting in an accelerated heart rate. The additional rise in blood pressure during the act of drinking appears to be a combination of excitement and sensory inputs from the pharyngeal region, causing a temporary activation of the sympathetic nervous system.

Animals↗

PubMed1999

Pharmacokinetics of fentanyl after intravenous and transdermal administration in goats.

OBJECTIVE: To evaluate disposition of fentanyl in goats after IV and transdermal administration. ANIMALS: 8 healthy 2-year-old goats weighing 31.8 to 53.6 kg (mean+/-SD, 40.4+/-7.5 kg). PROCEDURE: Each goat was given 2 treatments consisting of fentanyl administered IV (2.5 microg/kg of body weight) and via a transdermal patch (50 microg/h). There was a 2-month interval between treatments. Blood samples were collected at specified times and analyzed in duplicate to determine plasma fentanyl concentrations. Pharmacokinetic values were calculated, using a computerized modeling program. RESULTS: Administration of fentanyl was tolerated by all goats. Intravenous administration of fentanyl resulted in a transitory increase in rectal temperature that was not clinically important. Terminal elimination half-life after IV administration was 1.20+/-0.78 h, volume of distribution at steady state was 1.51+/-0.39 L/kg, and systemic clearance was 2.09+/-0.62 L/kg/h. Transdermal administration of fentanyl resulted in variable plasma concentrations, with peak plasma concentrations ranging from 1.12 to 16.69 ng/ml (mean+/-SD, 6.99+/-6.03 ng/ml) and time to peak concentration ranging from 8 to 18 hours (mean+/-SD, 13+/-4.5 hours). After removal of the transdermal patch, mean+/-SD terminal elimination half-life was 5.34+/-5.34 hours. CONCLUSIONS AND CLINICAL RELEVANCE: Intravenous administration of fentanyl (2.5 microg/kg) in goats results in a relatively short half-life that will limit its use for management of pain. Transdermal administration of fentanyl (50 microg/h) in goats results in variable plasma concentrations that may exceed those anticipated on the basis of a theoretical delivery rate, but stable plasma concentrations of fentanyl may not be achieved.

Administration, Cutaneous↗

PubMed1976

Comparative hematology: studies on goats.

Results of blood coagulation and hematologic studies on 6 goats, each tested 3 times, were compared to the values seen in persons. Special blood platelet studies were done on an additional goat. Blood coagulation values in the goats and in persons were similar, with these exceptions: In the goat, activated partial thromboplastin time was shorter and thrombin time was longer; one-stage assays of factors V, VIII, and IX were very high, and platelets aggregated poorly epinephrine and ristocetin. Both platelets and erythrocytes were small. On scanning electron microscopy, the erythrocytes appeared as flat disks or triangles, occasionally having a dimpled center, compared to the deeply dimpled doughnut shape of the larger human erythrocytes. Osmotic fragility of these small erythrocytes was greater than that of their human counterparts. By transmission electron microscopy of ultrathin sections of goat buffy coat, platelets had fine structures similar to those of human platelets. Unlike in human platelets, most of the dense bodies in goat platelets were surrounded by clear vacuoles. Biochemical studies showed higher than human levels of phosphorus, chloride, sodium, alkaline phosphatase, and serum glutamic oxaloacetic transaminase.

Animals↗

PubMed1991

Structural analysis of repetitive DNA sequences in the goat growth hormone gene region.

Two clones that contain goat growth hormone (gGH) genes were isolated from goat genomic library using goat growth hormone cDNA as a probe. One clone CgGH contained gGH1 gene, and another clone, EgGH, contained gGH2 and gGH3 genes. The clone EgGH contained 491 bp inserted sequence, just upstream of the gGH3 gene, which was not present in the clone CgGH. From the sequencing data of the flanking regions of these gGH genes, short interspersed repetitive sequences (SINES) were found. Four SINES's existed in the CgGH clone and eight SINES's existed in the EgGH clone. The number of the repetitive DNA sequences in the goat genome was estimated about 10(2) copies from the analysis of the reassociation rate. As the number of gGH genes (gGH1, gGH2, and gGH3) per genome was different among individual goats, DNA fragments containing gGH1 gene, and that containing gGH2 and gGH3 genes, were estimated to be allelic on the goat chromosome.

Animals↗

PubMed2005

Prediction of curve progression in a goat scoliosis model.

OBJECTIVES: Currently, prediction of progression in scoliosis is accomplished by analysis of several factors, which provide only a broad percentage chance, rather than an accurate risk assessment, of deformity progression. A model for prediction of scoliosis progression was investigated using an experimental scoliosis: A goat model was used to predict curve progression based on the percentage of vertebral body wedging in the region of maximal deformity. METHODS: Structural, lordoscoliotic curves of significant magnitude (> or = 30 degrees) convex to the right in the thoracic spine were created in 15 immature goats using a rigid posterior asymmetric tether in combination with convex rib resection and concave rib tethering. At 12 weeks, all posterior tethers were removed, and the goats were observed for an additional 4-week period. Serial radiographs were used to document progression (defined as > or = 5 degrees) and vertebral body wedging within the maximal scoliotic deformity. RESULTS: During the additional 4-week observation period following removal of the tether, seven goats developed progressive curves (mean progression: +10.1 degrees, range: +6 degrees to +17 degrees) and eight goats developed nonprogressive curves (mean: -1.6 degrees, range: -8 degrees to +4 degrees). At the beginning of the observation period, the percentage of vertebral body wedging was 60.4% versus 50.2% in the progressive versus nonprogressive groups (P = 0.002). Thus, at 55.3% vertebral body wedging, prediction of curve progression was possible for 85% of progressors and 88% of nonprogressors. CONCLUSIONS: Prediction of curve progression is often difficult when based on skeletal maturity and curve magnitude alone. In an immature goat scoliosis model, however, in which these two factors are relatively well controlled, curve progression can be predicted based on the percentage of vertebral body wedging in the region of maximal deformity.

Animals↗

PubMed2004

[The ht-PAm cDNA knock-in the goat beta-casein gene locus].

The production of recombinant protein is one of the major successes of biotechnology, animal cells are required to synthesize proteins with the appropriate post-translational modifications. Transgenic animal mammary gland bioreactor are being used for this purpose. Gene targeting is a more powerful method to produce mammary gland bioreactor, and nuclear transfer from cultured somatic cells provides an wonderful means of cell-mediated transgensis. Here we describe efficient and reproducible gene targeting in goat fetal fibroblasts to place the human tissue plasminogen activator mutant (ht-PAm) cDNA at the beta-casein locus, and would produce the transgenic goat by nuclear transfer. To construct the gene targeting vector pGBC4tPA, the milk goat beta-casein genomic DNA sequence for homologous arms had been cloned firstly. The left arm was 6.3 kb fragment including goat beta-casein gene 5' flanking sequence, and the right arm was 2.4 kb fragement including beta-casein gene from exon 8 to exon 9. The ht-PAm cDNA was subcloned in the goat beta-casein gene exon 2, and the endogenous start condon was replaced by that of ht-PAm. The bacterial neomycin (neo) gene as positive selection marker gene, was placed in the beta-casein gene intron 7, the thymidine kinase (tk) as the negative selection marker gene, was just outside the right arm. The validity of the positive-negative selection vector (PNS), was tested, and targeting homologous recombination (HR) were elevated to 5-fold with the negative selection marker using the drug GANC. The DNA fragment in which two LoxP sequence was delected effectively using Cre recombinase in vitro. Goat fetal fibroblasts were thawed and cultured to subconfluence before transfection, about 10(7) fibroblasts were electoporated at 240V, 600 microF in 0.8 mL PBS buffer containing linear pGBC4tPA. transfected cells were cultured in collagen-coated 96-wellplate for 24h without selection, then added the drug G418 (600 microg/mL) and GANC (2 micromol/L). After 12 days of selection, well separated G418r/GANCr clones were isolated and expanded in 24-wellplate. 244 clones were selected, and only 90 clones could grow and be tested by PCR screening for targeting. The primary result demonstrated that 31 targeting cell clones with homologous recombination events were obtained, and 2 cell clones was verified by DNA sequence analysis on the homologous recombination region.

Animals↗

PubMed1990

Determination of excretion of inulin, creatinine, sodium sulfanilate, and phenolsulfonphthalein to assess renal function in goats.

Excretion of creatinine, sodium sulfanilate (SS), and phenolsulfonphthalein (PSP) was studied in healthy goats. In conscious goats, mean (+/- SEM) inulin clearance was 2.26 +/- 0.08 ml/min/kg of body weight. Endogenous creatinine clearance, 1.97 +/- 0.09 ml/min/kg, underestimated inulin clearance (P less than 0.01), probably because of the presence of noncreatinine chromogens in caprine plasma. The estimated renal clearance of PSP was 6.88 +/- 0.39 ml/min/kg, whereas the estimated renal clearance of SS was 3.71 +/- 0.39 ml/min/kg. Both exceeded inulin clearance (P less than 0.01), confirming renal tubular secretion of both compounds. In 6 anesthetized goats, exogenous creatinine clearance and SS clearance exceeded inulin clearance (P less than 0.05). Results of stop-flow experiments documented secretion of creatinine and SS by the proximal portion of the caprine nephron. Plasma half-life of PSP in uninephrectomized goats exceeded that in intact goats (20.2 +/- 1.5 min vs 11.9 +/- 0.7 min; P less than 0.01). Similarly, plasma half-life of SS was greater in goats after uninephrectomy (58.2 +/- 6.2 min vs 30.4 +/- 1.2 min; P less than 0.01).

Animals↗