PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “Isometric Contraction”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 757 records · Page 42Linked to original sources
PubMed1997

Regulation of glycogen utilization, but not glucose utilization, by precontraction glycogen levels in vascular smooth muscle.

These experiments were designed to determine whether glycogenolysis was influenced by the glycogen concentration of vascular smooth muscle. Segments of hog carotid artery smooth muscle were allowed to synthesize variable amounts of 1-[13C]glucosyl units of glycogen. Artery segments were then isometrically contracted in the presence of 2-[13C]glucose. Prior to and after isometric contraction, measurements were made of tissue glycogen content and superfusate glucose and lactate concentrations. 2-[13C]Lactate and 3-[13C]lactate peak intensities in the superfusate were measured using 13C-NMR spectroscopy. The tissue glycogen content decreased exponentially during the 4.5 h of isometric contraction (R2 = 0.990), despite more than a 3-fold range of glycogen concentration prior to contraction. The extent of glycogen utilization during a 3 h isometric contraction varied linearly with the precontraction glycogen concentration (R2 = 0.727). Lactate production specifically from glycogen breakdown increased with an increase in precontraction glycogen concentration (R2 = 0.620). During a 3 h isometric contraction neither the glucose utilization (R2 = 0.007) nor lactate production specifically produced from glucose (R2 = 0.00002) varied with the precontraction glycogen concentration. It is concluded that the rate of glycogenolysis is determined by the content of glycogen during prolonged contractions. In addition, precontraction glycogen levels influence the pathway for glycogen utilization but not the pathway for glucose utilization. Therefore, glycolysis and glycogenolysis behave independently in vascular smooth muscle.

Animals↗
PubMed1996

Changes in R-R variability before and after endurance training measured by power spectral analysis and by the effect of isometric muscle contraction.

The long-term conditioning effects of physical training on cardiorespiratory interaction in 11 young healthy males were studied. Significant increases in maximum oxygen uptake (VO2max) (P < 0.05) and decreases in heart rate (P < 0.05) were achieved in all subjects following a 6-week training programme consisting of cycling for 25 min each day at a work level that increased heart rate to 85% of maximum. Heart rate variability, measured as the differences between the maximum and minimum R-R interval in a respiratory cycle, increased in nine of the subjects and decreased in two. The respiratory-cycle-related high-frequency peak in the power spectral plot of R-R variability also showed significant increases in the same nine subjects and decreases in two. The latter result was similar after normalisation of the data for changes in heart rate by calculating the common coefficient of variance [symbol: see text], where HF is the high-frequency component of the power spectral plots, using a further measure of vagal tone it was shown that, for all subjects, the R-R interval change in response to isometric contractions of the arm flexors in one respiratory cycle were significantly greater after training. These data suggest that cardiac vagal tone is increased by aerobic training for all subjects and that this is accompanied by a respiratory sinus arrhythmia (RSA) in most, but may be associated with a decrease in RSA in subjects with a very low (< 50 beats.min-1 heart rate.

Adult↗
PubMed2000

Lumbar erector spinae oxygenation during prolonged contractions: implications for prolonged work.

Owing to the recent interest in torso stabilization exercises together with many questions regarding the duration of prolonged isometric holds in occupational settings, the authors attempted to assess the level of back muscle oxygenation during prolonged isometric contractions. Specifically, this study recorded relative oxygen saturation of haemoglobin/myoglobin using Near Infrared Spectroscopy (NIRS) in the L3 erector mass during prolonged isometric contractions at intensities from 2 to 30% of maximum voluntary contraction (MVC). It was hypothesized that available oxygen to these muscles is severely compromised even at moderate levels of activation observed in occupational work. Eight volunteers without a history of lower back pain or injury participated in this study. The exercise task involved isometric contraction of the lower erector spinae at five different levels of each subject's maximal voluntary contraction: 2, 5, 10, 20 and 30% MVC, presented in random order. Subjects were placed in a sitting position, with a curved plastic plate secured horizontally to the pelvis to minimize movement at the hip joint. During extensor exertions, they were restrained with a harness that was attached at chest level to a load cell. Each isometric contraction was performed for 30 s followed by 1 min of rest. All levels of contraction demonstrated reduction in oxygen. Given the concern for motion artefact on the NIRS signal, sham trials were conducted where the subjects went through the procedure of attaching the pulling cable but no active pull was performed. These trials showed no change in the NIRS signal. At this time NIRS appears to be the only non-invasive instrumentation available to indicate total available muscle oxygen during low level, prolonged work. Although the specific tissue volume sampled by NIRS cannot be positively identified, it appears that tissue oxygenation in the lumbar extensor musculature is reduced as a function of contraction intensity, even at levels as low as 2% of MVC. These data have implications for prolonged work where postures requiring isometric contractions are sometimes held for hours, and where musculoskeletal illness has been linked to prolonged contraction levels above 2%MVC--these data suggest a possible biological pathway.

Adult↗
PubMed2006

Contrast of directional influence upon motor overflow between submaximal and maximal static exertions.

This study investigated exertion-dependent motor overflow among healthy adults when they performed isometric tasks with contralateral joints in different task directions. Twenty healthy adults (10 males and 10 females, mean age = 26.2 yrs) were instructed to complete a set of isometric contractions of various force vectors with the shoulder, elbow, and wrist joints, in a total of ten motor tasks at submaximal and maximal intensities (50%, 100% maximal voluntary contractions). The electromyographical activities from eight muscles of the unexercised upper limb were recorded to characterize intensity of motor overflow during sustained isometric contraction. Both occurrence frequency and magnitude of motor overflow in terms of standardized net excitation (SNE) increased with exertion level for all joint movements (P < 0.001). Additionally, the motor overflow magnitude depended strongly on the task direction of maximal isometric contraction (P < 0.05). Motor overflow was particularly augmented by the contralateral isometric contractions where task directions were opposed to gravity. However, such a directional effect upon SNE was not evident during submaximal contraction (P > 0.05). The difference of the net excitation between maximal and submaximal contraction (DNE(100%-50%MVC) data) indicated that the pectoralis major and triceps brachii consistently exhibited a marked recruitment in reaction to change in task direction of isometric contraction. Patterned motor overflow may be physiologically relevant to topological mapping of the ipsilateral pathways and altered effectiveness of use-dependent interhemispherical connectivity. The current observations provide better insight into gain in muscle strength due to contralateral exercise.

Adult↗
PubMed1998

The isometric force that induces maximal surface muscle deoxygenation.

To determine the external force that induces maximal deoxygenation of brachioradialis muscle 32 trained male subjects maintained isometric contractions using the elbow flexor muscles up to the limit time (isotonic part of the isometric contraction, IIC) and beyond that time for 120 s (anisotonic part of the isometric contraction). During IIC each subject maintained relative forces of either 25% and 70% maximal voluntary contraction (MVC), 50% and 100% MVC, or 40% and 60% MVC. Muscle oxygenation was assessed using a near infrared spectroscope, and expressed as a percentage of the reference value (deltaO2rest) which was the difference between the minimal oxygenation obtained after 6 min of ischaemia at rest and the maximal reoxygenation following the release of the tourniquet. During IIC at 25% MVC, muscle oxygenation decreased to 17 (SEM 3)% deltaO2rest, then it levelled off [25 (SEM 1)% deltaO2rest]. After the point at which target force could not be maintained, reoxygenation was very weak. During IIC at 40%, 50%, 60%, and 70% MVC, the lowest muscle oxygenation values were obtained after 15-20 s of contraction and corresponded to -18 (SEM 6), -59 (SEM 12) -31 (SEM 6), and -29 (SEM 6)% deltaO2rest, respectively. For the contraction at 100% MVC, the lowest oxygenation [-19 (SEM 9)% deltaO2rest] was obtained while force was decreasing (69% MVC). During the anisotonic part of the isometric contractions, the greatest reoxygenation rate was obtained after 50% MVC IIC (P < 0.001). Our results showed that during isometric elbow flexions between 25% and 100% MVC, there was no linear relationship between external force and muscle oxygenation, and that the maximal deoxygenation of the brachioradialis muscle was obtained at 50% MVC.

Adult↗
PubMed1977

Relaxation of heart muscle by catecholamines and by dibutyryl cyclic adenosine 3',5'-monophosphate. Similarity of beta-adrenoceptors mediating contractile and relaxant effects of catecholamines in kitten pipillary muscle.

1. In isometrically contracting kitten papillary muscles, dibutyryl cyclic AMP (DBcAMP) enhanced peak tension, increased rates of contraction and relaxation, decreased tension of a phasic but not of a small tonic component of KCl-contractures, and caused aftercontractions. These effects resemble closely those of catecholamines. 2. The effects of DBcAMP on kitten papillary muscle were not influenced by (-)-bupranolol, a beta-adrenoceptor antagonist. 3. DBcAMP decreased KCl-contractures in strips of frog ventricle. 4. Phasic KCl-contractures in kitten papillary muscles were decreased by (-)- and (+)-isoprenaline. For similar effects, 100-fold higher concentrations of (+)-isoprenaline than of (-)-isoprenaline were required. 5. Increases in maximum rates of contraction and relaxation, increases in peak tension of isometric contractions and reduction of phasic KCl-contractures by catecholamines were antagonized competitively to a similar extent by (-)-bupranolol. Mean apparent equilibrium constants for the beta-adrenoceptor-(-)-bupranolol complex of 0.46-0.70 nM were estimated. These constants were quite similar irrespective of whether (-)-isoprenaline, (+)-isoprenaline or (-)-noradrenaline were used as agonists. 6. Increases in contractile strength, maximum rates of contraction and of relaxation of isometric contractions and decreases in KCl-contractures by (-)-isoprenaline were surmountably blocked by (+)-bupranolol. Mean apparent equilibrium constants for the receptor-(+)-bupranolol complex were 40-50 nM. 7. The equilibrium constants of (-)- and (+)-bupranolol for the receptors mediating positive inotropic and relaxant effects of catecholamines were not significantly different from constants for bupranolol-receptor complexes in cell-free membrane particles of kitten heart ventricle. It is suggested that the same beta-adrenoceptor triggers positive inotropic, relaxant and adenylyl cyclase-activating effects of catecholamines in kitten papillary muscle. 8. The partial agonist (-)-dichloroisoprenaline (DCI) (1 muM) reduced by 79% the phasic KCl-contractures of the kitten papillary muscles. DCI stimulates adenylyl cyclase activity of ventricle membranes to less than 1/4 of maximum stimulation by (-)-isoprenaline. If cyclic AMP produced by DCI is involved in the decrease of phasic KCl-contracture, small increase in cyclic AMP should be sufficient to induce this effect.

Adrenergic beta-Antagonists↗
PubMed1996

Prediction of prostatic obstruction with a combination of isometric detrusor contraction pressure and maximum urinary flow rate.

OBJECTIVES: Because isometric detrusor contraction pressure (Piso) increases with outlet obstruction and maximum urinary flow rate (Qmax) tends to decrease with obstruction, we hypothesize that specific criteria consisting of a combination of high Piso and low Qmax may be able to differentiate obstructive from nonobstructive voiding dysfunction better than either parameter alone. METHODS: Two hundred five men with lower urinary tract symptoms underwent uroflowmetry and videourodynamics, including cystometry, continuous outlet occlusion test, and micturitional urethral pressure profilometry. Combined threshold values of Qmax of less than 12 mL/s and Piso of 100 cm H2O or greater were used to predict obstruction, whereas threshold values of Qmax of at least 12 mL/s and Piso less than 100 cm H2O were used to predict nonobstruction. RESULTS: Of the 205 patients, 103 (50%) were significantly obstructed and 102 (50%) were only mildly obstructed or nonobstructed. Of the total population, 151 patients (74%) were categorized by the combined flow and contractility criteria. Of the categorized patients, 141 (93%) were correctly diagnosed with regard to infravesical obstruction (sensitivity 89%, specificity 97%, positive predictive value 97%, and negative predictive value 91%). CONCLUSIONS: A combination of Qmax and Piso criteria can predict obstructive and nonobstructive voiding dysfunctions with high positive and negative predictive values in most patients with lower urinary tract symptoms. Combining the results of uroflowmetry and isometric tests may help to guide treatment strategies that may improve the outcome of selected therapeutic options compared with strategies based on symptoms or uroflowmetry alone. Furthermore, this approach forms a basis for interpreting various noninvasive methods that have recently been introduced for the purpose of diagnosing bladder outlet obstruction.

Aged↗
PubMed2006

The physiology of rock climbing.

In general, elite climbers have been characterised as small in stature, with low percentage body fat and body mass. Currently, there are mixed conclusions surrounding body mass and composition, potentially because of variable subject ability, method of assessment and calculation. Muscular strength and endurance in rock climbers have been primarily measured on the forearm, hand and fingers via dynamometry. When absolute hand strength was assessed, there was little difference between climbers and the general population. When expressed in relation to body mass, elite-level climbers scored significantly higher, highlighting the potential importance of low body mass. Rock climbing is characterised by repeated bouts of isometric contractions. Hand grip endurance has been measured by both repeated isometric contractions and sustained contractions, at a percentage of maximum voluntary contraction. Exercise times to fatigue during repeated isometric contractions have been found to be significantly better in climbers when compared with sedentary individuals. However, during sustained contractions until exhaustion, climbers did not differ from the normal population, emphasising the importance of the ability to perform repeated isometric forearm contractions without fatigue becoming detrimental to performance. A decrease in handgrip strength and endurance has been related to an increase in blood lactate, with lactate levels increasing with the angle of climbing. Active recovery has been shown to provide a better rate of recovery and allows the body to return to its pre-exercised state quicker. It could be suggested that an increased ability to tolerate and remove lactic acid during climbing may be beneficial. Because of increased demand placed upon the upper body during climbing of increased difficulty, possessing greater strength and endurance in the arms and shoulders could be advantageous. Flexibility has not been identified as a necessary determinant of climbing success, although climbing-specific flexibility could be valuable to climbing performance. As the difficulty of climbing increases, so does oxygen uptake (VO(2)), energy expenditure and heart rate per metre of climb, with a disproportionate rise in heart rate compared with VO(2). It was suggested that these may be due to a metaboreflex causing a sympathetically mediated pressor response. In addition, climbers had an attenuated blood pressure response to isometric handgrip exercises when compared with non-climbers, potentially because of reduced metabolite build-up causing less stimulation of the muscle metaboreflex. Training has been emphasised as an important component in climbing success, although there is little literature reviewing the influence of specific training components upon climbing performance. In summary, it appears that success in climbing is not related to individual physiological variables but is the result of a complex interaction of physiological and psychological factors.

Anthropometry↗
PubMed2006

Ultrasonographic evaluation of functioning free muscle transfer: comparison between spinal accessory and intercostal nerve reinnervation.

This study focuses on the application of ultrasonography as a means of measuring the cross-sectional area (CSA) of the transferred muscle and evaluating its force recovery following functioning free muscle transfer. The objective of the study was to compare the CSA of a transferred muscle that is either reinnervated by the spinal accessory nerve (SAN) or the intercostal nerve (ICN), and to evaluate the difference in their force recovery. Ten patients with complete avulsion of the brachial plexus who underwent a double free muscle technique for restoring prehensile function were evaluated. All patients were followed up for at least 1.5 years after the operation. The CSAs of 20 transferred gracilis muscles in 10 patients, reinnervated either by SAN or ICN, were measured by ultrasonography. The CSA was measured at relaxation and at maximal isometric contraction. The force recovery of each muscle was expressed as the contraction rate (CR), calculated by dividing the CSA of the muscle in maximum isometric contraction by the CSA of the muscle in relaxation. The mean CSA of the transferred muscles reinnervated by the SAN was 2.98 +/- 0.723 cm (2) in relaxation and 3.95 +/- 1.296 cm (2) in maximum isometric contraction; thereby a CR of 1.32 +/- 0.174 was obtained. The mean CSA of the transferred muscles reinnervated by the ICN was 2.32 +/- 0.520 cm (2) and 2.69 +/- 0.566 cm (2) in relaxation and maximal isometric contraction, respectively; thus a CR of 1.16 +/- 0.068 was obtained. Results showed that the CR was significantly higher among the transferred muscles reinnervated by the SAN than those by the ICN. This study demonstrated that muscles that are reinnervated by the SAN resulted in stronger recovery than those reinnervated by the ICN, and that ultrasonography has the capacity to evaluate force recovery of each muscle by measuring the CSA during the two phases of muscle activity.

Accessory Nerve↗
PubMed1985

Brain potentials related to the beginning and to the termination of voluntary flexion and extension in man.

The presence of brain potentials related to the end of a sustained isometric contraction prompted this study. Potentials related to the beginning of simple ballistic flexion of the index finger were compared with isometric contraction, potentials related to the termination of the isometric contraction, and potentials related to ballistic extension of the reposed index finger. The electroencephalograms recorded in 10 healthy volunteers from scalp positions C3+2, Cz, C3-2 were averaged time-locked to the appropriate mechanograms. Both negativity preceding and positivity following execution of movement were better expressed with simple flexion movements. The negativities preceding all four types of movements were of comparable magnitude whilst the positivities differed significantly. These findings were considered as supporting the ideas of general facilitation preceding preparation for action and specific feedback information following execution, the changes being more prominent over postcentral leads. The existence of potentials related to the termination of voluntary sustained isometric contraction implied a separate command for antagonistic muscles.

Adult↗
PubMed1981

Relationship between force and integrated EMG activity during voluntary isometric anisotonic contraction.

The surface electromyogram (EMG) was recorded for the extensor carpi radiales of normal human subjects during voluntary isometric anisotonic contractions as well as the force generated by the muscle. The relationship between force and instantaneous integrated EMG could not be fitted by a single linear function or a parabola. The muscle was then considered as a system (A) presenting a maximum force and (B) in which the EMG activity represented the input and the contraction force the output, that is F = f(iEMG) and not, as is usually done, iEMG = f(F). In addition the summed iEMG from the beginning of the contraction was used since the actual force depends on all preceding events. These findings let to testing for a non-linear and asymptotic function relating F to iEMG. A double exponential function: (formula: see text) could account for the two mechanisms by which the force of a voluntary contraction is increased.

Calcium↗
PubMed1991

Conformations of crossbridges in contracting skeletal muscle.

Conformations of crossbridges during isometric contraction were observed and compared with those in rigor state. Rabbit skeletal muscle fibers were chemically skinned, rapidly frozen during isometric contraction or in the rigor state, then freeze-substituted. Longitudinal and cross sections were observed. Crossbridges in contracting fibers are arranged on the thin filament, retaining a periodicity of approximately 14.3 nm with some variability, while crossbridges in rigor fibers tend to attach to actin target zones which have a periodicity of approximately 36 nm. Rigor crossbridges are wide near the thin filament; crossbridges in contracting fibers are thinner and have more uniform width from the proximal region to the distal region. The majority of the mass of the crossbridges during contraction is not as close to the thin filament as in rigor fibers. Shapes of crossbridges during contraction are variable, especially when viewed along the filament axis.

Actin Cytoskeleton↗
PubMed1996

Central changes in muscle fatigue during sustained submaximal isometric voluntary contraction as revealed by transcranial magnetic stimulation.

Changes in responses to transcranial magnetic stimulation (TMS) during submaximal isometric voluntary contraction (60% of maximal voluntary contraction (MVC) of the adductor pollicis muscle and the subsequent recovery period have been studied in healthy volunteers. TMS at twice the motor threshold was applied during the sustained contraction, as well as at rest and during short-lasting (2 s) 60% MVCs before and immediately after the sustained contraction, and at 5 min intervals during the recovery period. Both motor evoked potential (MEP) magnitude (peak and area) and silent period (SP) duration in electromyographic activity (EMG) of the adductor pollicis muscle showed a gradual decrease up to the endurance point and an increase thereafter. MEPs elicited at rest immediately after the fatiguing contraction were larger, whereas those elicited later on during the recovery period did not differ significantly from the controls. It is suggested that the changes in responses to TMS, divergent from those in ongoing voluntary EMG during the sustained 60% MVC, indicate complex processes at levels preceding the motor cortex output cells in an attempt to maintain a submaximal contraction of the fatigued muscle. The increase in MEP magnitude after the sustained 60% MVC may indicate residual changes in cortical activity after fatiguing contraction.

Adult↗
PubMed2005

Firing rates of motor units during strong dynamic contractions.

Muscle behavior is usually studied during isometric contractions but many tasks include contractions that involve changes in muscle length. Our aim was to record motor unit action potentials and surface electromyograms (EMGs) from triceps brachii muscles during rhythmic dynamic contractions (3-s concentric, 3-s eccentric; 40 degrees/s; four subjects) performed at the highest voluntary forces subjects could exert (maximal concentric contraction) and at various submaximal intensities. Mean unit firing rates and surface EMG increased significantly with contraction intensity in both concentric and eccentric contractions, but at each intensity mean concentric values were significantly higher than eccentric values. In contrast, mean unit firing rates and surface EMGs were similar during maximal concentric and maximal isometric contractions. These data suggest muscles were activated maximally during the strongest concentric contractions but submaximally during the strongest eccentric efforts. After estimated eccentric contraction intensity was adjusted using surface EMG data, mean unit firing rates during eccentric contractions were still lower than the concentric values. Thus, protective mechanisms may limit motor unit firing rates during forceful lengthening contractions to minimize damage.

Action Potentials↗
PubMed1997

Studies on the vasoconstrictor action of melatonin and putative melatonin receptor ligands in the tail artery of juvenile Wistar rats.

1. In this study we compared the vasoconstrictor activity of melatonin in rat isolated tail artery using two different recording systems, the Halpern pressure myograph and the Halpern-Mulvany wire myograph, with the view to determining a reliable method for obtaining pharmacological data on vascular melatonin receptors. In addition, we characterized the melatonin receptor in this preparation, using analogues of melatonin, and examined the activity of various naphthalenic derivatives with biological activity in non-vascular models of melatonin receptors. 2. Using the Halpern pressure myograph, cumulative addition of melatonin (0.1 nM to 1 microM) produced direct vasoconstriction (19.3+/-6.4% reduction in lumen diameter, n=5) in five of 11 pressurized segments, with pEC50 of 9.14+/-0.17. Similarly, non-cumulative application of melatonin caused vasoconstriction (19.7+/-4.6% reduction in lumen diameter, n=7) in seven of 20 preparations examined with pEC50 of 8.74+/-0.26. The selective alpha2-adrenoceptor agonist, UK-14304 (5-bromo-6-[2-imidazolin-2-ylamino]-quinoxaline bitartrate), produced vasoconstriction in all 'melatonin-insensitive' preparations. 3. Melatonin (0.1 nM to 1 microM) failed to elicit isometric contractions of tail artery segments in the Halpern wire myograph, but produced concentration-dependent potentiation of electrically-evoked, isometric contractions (maximum effect of 150-200% enhancement) when applied either noncumulatively (seven of seven preparations) or cumulatively (four of seven preparations). The pEC50 value of melatonin (non-cumulative) was 8.50+/-0.10 (n=7) which was not different from that obtained in the pressure myograph. All further experiments were conducted using a non-cumulative protocol against electrically-evoked, isometric contractions. 4. Based on the pEC50 values for the melatonin analogues examined, the pharmacological profile for the enhancement of electrically-evoked contractions was 2-iodomelatonin > 6-chloromelatonin > or = (-)-AMMTC > or = S21634 > or = melatonin > or = S20098 > S20242 > or = S20304 > 6-hydroxymelatonin > S20932 > (+)-AMMTC > N-acetyl-5-HT. Our data suggests the vascular receptor belongs to the MEL1-like subtype. All the indole-based analogues of melatonin, 2-iodomelatonin, (-)-AMMTC, (+)-AMMTC, S20932, 6-chloromelatonin, 6-hydroxymelatonin and N-acetyl-5-HT, behaved as full agonists. All the naphthalenic derivatives examined, S21634, S20098, S20242 and S20304 behaved as partial agonists relative to melatonin. 5. The naphthalenic-based antagonists, S20928 and S20929, did not modify electrically-evoked, isometric contractions of the tail artery, but produced a parallel, rightward displacement of the melatonin concentration-response curve. Based upon the effect of 1 microM S20928 and S20929, the estimated pK(B) values for these antagonists were 7.18+/-0.25 (n=4) and 7.17+/-0.25 (n=5), respectively. 6. We demonstrated that enhancement of electrically-evoked, isometric contractions of the rat isolated tail artery (using the Halpern-Mulvany wire myograph) is a simple and reproducible model for assessing the activity of putative agonists, partial agonists and antagonists at vascular melatonin receptors. Pharmacological characterization of the receptor suggests the presence of a MEL1-like subtype.

Animals↗
PubMed1995

Prejunctional actions of K+ channel blockers in rat vas deferens.

In studies of isometric contractions in prostatic portions of rat vas deferens evoked by single pulse electrical stimulation, the K+ channel blockers 4-aminopyridine, tetraethylammonium and charybdotoxin, but not apamin, significantly reduced the prejunctional inhibitory potency and the maximum inhibitory effect of the alpha 2-adrenoceptor agonist xylazine. The protein kinase C activator phorbol dibutyrate had similar effects to 4-aminopyridine against xylazine. However, 4-aminopyridine, tetraethylammonium, charybdotoxin and phorbol dibutyrate, but not apamin, significantly increased the magnitude of the isometric contraction to a single stimulus. 4-Aminopyridine and phorbol dibutyrate significantly reduced, while tetraethylammonium did not affect, isometric contractions to noradrenaline, and 4-aminopyridine failed to affect contractions to alpha,beta-methylene-ATP, so that the effects of these agents on the isometric contraction to a single stimulus were presumably by a prejunctional action. The Ca2+ entry facilitator Bay K 8644 (1,4-dihydro-2,6-dimethyl-5-nitro-4-[2-(trifluoromethyl)-phenyl]-3-pyrid ine carboxylic acid methylester) increased stimulation-evoked contractions by a postjunctional action and reduced the inhibitory effects of xylazine. When the isometric contraction following 4-aminopyridine was reduced by decreasing the stimulation voltage or by reducing the Ca2+ concentration from 2.5 to 0.9 mM, 4-aminopyridine significantly reduced the potency of xylazine. However, tetraethylammonium and Bay K 8644 failed to affect the inhibitory potency of xylazine in low Ca2+. It is concluded that the K+ channel blocker 4-aminopyridine reduces the prejunctional inhibitory potency of xylazine, and this action is independent of increased neurotransmitter release. These results suggest that prejunctional alpha 2-adrenoceptor-mediated inhibition in rat vas deferens involves K+ channels sensitive to block by 4-aminopyridine.

3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethy↗
PubMed2006

Examination of EMG normalisation methods for the study of the posterior and posterolateral neck muscles in healthy controls.

The purpose of this study was to examine the reliability of normalisation methods used in the study of the posterior and posterolateral neck muscles in a group of healthy controls. Six asymptomatic male subjects performed a total of 12 maximum voluntary isometric contractions (MVIC) and 60%-submaximal isometric contractions (60%-MVIC) against the torque arm of an isokinetic dynamometer whilst surface and intramuscular electromyography (EMG) was recorded unilaterally from representative posterior and posterolateral locations. Reliability was calculated using intra-class correlation coefficient (ICC), relative standard error of measurement (%SEM) and relative coefficient of variation (%CV). Maximal torque output was found to be highly reliable in the directions of extension and right lateral bending when the first of three MVIC contractions was excluded. When averaged across contraction direction, high reliability was found for both surface (MVIC: ICC=0.986, %SEM=7.5, %CV=9.2; 60%-MVIC: ICC=0.975, %SEM=10, %CV=13.7) and intramuscular (MVIC: ICC=0.910, %SEM=20, %CV=19.1; 60%-MVIC: ICC=0.952, %SEM=16.5, %CV=13.5) electrodes. Intramuscular electrodes displayed the least reliability in right lateral bending. The use of visual feedback markedly increased the reliability of 60%-MVIC contractions.

Adult↗