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PubMed1979

Induction of blastogenesis in bovine peripheral blood lymphocytes by oxidation with sodium periodate.

Suitable treatment and culture conditions are defined for the induction of blast transformation in bovine peripheral blood lymphocytes by oxidation with sodium metaperiodate (NaIO4). Stimulation with NaIO4 required slight modification of techniques used routinely for activation of lymphocytes in vitro with lectins and antigens. Gradient-separated mononuclear leukocytes responded with maximal [3H]TdR incorporation after oxidation with 0.50 to 1.0 mM NaIO4 for 30 minutes at 25 C. Oxidized cells cultured at 1 to 2 X 10(6)/ml responded better than cells cultured at any other concentration, when compared with untreated cells. Blastogenesis in response to oxidation reached its maximum rate within 48 hours of treatment, after which it declined rapidly. Partial removal of glass wool-adherent cells reduced periodate-triggered blastogenesis by 95%, but did not significantly affect activation with phytohemagglutinin, concanavalin A, pokeweed mitogen, or purified protein derivative. Reintroduction of macrophages restored responses to their precolumn level. Oxidation with NaIO4 provided a simple, rapid means of inducing blastogenesis in bovine lymphocytes. Manipulation of the well-defined triggering conditions may help to explain the mechanisms involved in lymphocyte activation.

Animals↗
PubMedDate not supplied

[Effect of periodate oxidation on activity of acid alpha-glucosidase from human liver].

Treatment with sodium metaperiodate caused oxidation of carbohydrate residues in acid alpha-glucosidase (gamma-amylase). Efficiency of the oxidation was monitored by a decrease in specific binding of alpha-glucosidase with concanavaline A-Sepharose. The degree of oxidation reached 96% in a medium containing 0,011 M NaIO4, pH 5.5, within 120 min. The residual activity of oxidized alpha-glucosidase was about 77%. The rate of oxidation and residual enzymatic activity were shown to depend also on concentration of sodium metaperiodate and duration of the reaction.

Chemical Phenomena↗