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The reproducibility of multi-joint isokinetic and isometric assessments in a healthy and patient population.

DESIGN: Test re-test reliability design. OBJECTIVE: To determine a reproducible protocol for lower limb simultaneous multi-joint assessments on a healthy group of subjects and a patient group with patellofemoral pain syndrome. BACKGROUND: Although single joint assessment with isokinetic dynamometers has been utilised for many years in knee rehabilitation, simultaneous multi-joint assessment has not attracted comparable attention. METHODS: Twenty healthy volunteers mean age 30.6 years and 16 patients with patellofemoral pain syndrome mean age 29.6 years performed isometric maximum voluntary contractions and concentric isokinetic contractions of the lower limb. Data were collected on three separate days for resultant extension/flexion peak torque, average power and total work which were analysed using intraclass correlation coefficients with a 95% level of confidence. RESULTS: In healthy subjects, intraclass correlation coefficients estimates were > or =0.75 for isokinetic peak torque and >0.83 for average power and total work. The intraclass correlation coefficients estimate for isometric peak torque extension was 0.82. In the patient group, intraclass correlation coefficients estimates were >0.82 for isokinetic peak torque and > or =0.75 for average power and total work. The intraclass correlation coefficients estimate for isometric peak torque extension was 0.89. Discarding the first session's data for both groups improved the intraclass correlation coefficients estimates for virtually all assessments. CONCLUSIONS: This study has demonstrated high reproducibility of lower limb multi-joint testing for peak torque, average power, and total work on healthy subjects and then has employed the protocol to demonstrate similarly high reliability on a patient group. It has also highlighted the need for a practice session before the data can be said to be reliable. RELEVANCE: Reliability studies often use healthy subject groups, which although useful, have limited relevance to patient populations. This study has not only gained comprehensive reproducibility data on multi-joint assessment in healthy subjects, it has also shown that multi-joint testing can be used safely and reliably in patients with patellofemoral pain syndrome. Clinicians may now be able to measure objectively lower limb function in this patient group both pre and post treatment.

Adult↗

Relative contribution of trunk muscles to the stability of the lumbar spine during isometric exertions.

OBJECTIVE: To compare the relative contribution of various trunk muscles to the stability of the lumbar spine. DESIGN: Quantification of spine stability with a biomechanical model. BACKGROUND: Modern low back rehabilitation techniques focus on muscles that stabilize the lumbar spine. However, the relative contribution of various trunk muscles to spine stability is currently unknown. METHODS: Eight male subjects performed isometric exertions in trunk flexion, extension, lateral bending, and axial rotation, and isometric exertions under vertical trunk loading and in a lifting hold. Each isometric trial was repeated three times at 20%, 40%, and 60% of the maximum trunk flexion force or with a load of 0%, 20%, 40%, and 60% of body weight for the latter two exertions. Surface EMG data from 12 major trunk muscles were used in the biomechanical model to estimate stability of the lumbar spine. A simulation of each trial was performed repeatedly with one of the 10 major trunk muscle groups removed from the model. RESULTS: Relative contribution of each muscle to spine stability was significantly affected by the combination of loading magnitude and direction (3-way interaction). None of the removed muscles reduced spine stability by more than 30%. CONCLUSIONS: A single muscle cannot be identified as the most important for the stability of the lumbar spine. Rather, spine stability depends on the relative activation of all trunk muscles and other loading variables. RELEVANCE: This study will improve our understanding of individual trunk muscles' contribution to overall stability of the lumbar spine.

Adult↗

Non-standard O(2) consumption-temperature curves during rest and isometric exercise in human skeletal muscle.

The present work was aimed at measuring intramuscular oxygen consumption (O(2)) as a function of temperature (T), in human forearm, during rest and aerobic isometric exercise (4% of the maximal voluntary contraction, MVC). Based upon results from in vitro experiments performed on isolated mitochondria of animal species, it was hypothesised that, during isometric exercise, the O(2)-T curve should display a maximum for some 'optimal' T. Intramuscular T and measurements were performed using a combined deep body temperature/near infrared probe during muscle cooling. At rest, O(2) increased non-linearly and monotonically as a function of T (n=8). O(2) increased approximately 2 times when going from 26 to 36 degrees C. A log(O(2))-T plot or a log(O(2))-1/T did not linearise the data. During isometric contraction, O(2) values at 26.8+/-0.6, 28.6+/-0.9, 31.9+/-0.9 and 35.9+/-0.9 degrees C were 3.04+/-1.26, 7.60+/-1.64, 4.43+/-1.95, and 6.64+/-1.37 micromol 100 g(-1) min(-1), respectively (n=6). The O(2) value at 28.6 degrees C was significantly higher (P<0.05) than that at 26.8 and 31.9 degrees C. The 'sudden' O(2) change at 28.6 degrees C is compatible with the phenomenon observed at the mitochondrial level.

Animals↗

Is there a relationship between muscle fatigue resistance and cardiovascular responses to isometric exercise in mild chronic heart failure?

BACKGROUND: Exercise intolerance in chronic heart failure (CHF) may be due to altered fatigue resistance and muscle afferent input to the cardiovascular system from dysfunctional skeletal muscle. AIM: To determine whether calf muscle fatigue resistance was associated with the magnitude of a muscle afferent driven cardiovascular response to isometric exercise. METHODS AND RESULTS: Cardiovascular responses were recorded in eight stable CHF patients (ejection fraction 20-40%) and nine healthy, age-matched controls during voluntary and electrically evoked isometric plantar flexion and post-exercise circulatory occlusion. The force developed by the plantar flexors during a 2-min submaximal electrically evoked fatigue test was measured. There was no relationship between ischaemic muscle fatigue and cardiovascular changes during and after voluntary contraction in either group nor evoked contraction in the CHF group. In the control group, the change in diastolic blood pressure (DBP) at the end of evoked contraction was related to the severity of fatigue at 90 s and 120 s (FI=0.01DeltaDBP+0.3, r=0.81, P<0.05 and FI=0.02DeltaDBP+0.8, r=0.84, P<0.01, respectively). CONCLUSION: Muscle fatigue resistance did not relate to the magnitude of the cardiovascular stress generated by isometric exercise of the same muscle in these patients.

Blood Pressure↗

Haemodynamic response to an isometric exercise test in obese patients: influence of autonomic dysfunction.

OBJECTIVE: To investigate blood pressure (BP) and heart rate (HR) responses to an isometric exercise test in obese non diabetic patients and to correlate the results with vagal function and plasma insulin concentration. SUBJECTS: 63 obese patients, 36 of whom had abnormal cardiac parasympathetic control (PS+), and 35 healthy control subjects. METHODS: Analysis of HR variations during three standardized tests: deep-breathing, lying-to-standing and Valsalva. Isometric contraction (handgrip) for 5 min. RESULTS: In the PS+ obese patients, resting HR and body mass index (BMI) were significantly higher than in the PS- subjects and there was a trend to higher plasma insulin concentrations. Age-matched comparison showed that during the handgrip test, the increase in HR at the first minute was significantly higher in the PS- obese patients than in the controls. The increase in BP was significantly lower in the PS+ obese patients than in age-and-BMI-matched PS- obese patients. CONCLUSION: These data suggest that 1) there is an increase in cardiac vagal tone in PS- obese patients, since the early increase in HR at 1 min of the handgrip test, results from the withdrawal of vagal tone; 2) BP response to an isometric contraction is impaired in PS+ obese patients due to a lower sympathetic activation; 3) high plasma insulin concentrations may also contribute to limiting the BP response; and 4) autonomic disorders may account for alterations in the haemodynamic changes during exercise.

Adolescent↗

Creatine kinase equilibrium and lactate content compared with muscle pH in tissue samples obtained after isometric exercise.

Muscle biopsies taken from the musculus quadriceps femoris of man were analysed for pH, ATP, ADP, AMP, creatine phosphate, creatine, lactate and pyruvate. Biopsies were taken at rest, after circulatory occlusion and after isometric contraction. Muscle pH decreased from 7.09 at rest to 6.56 after isometric exercise to fatigue. Decrease in muscle pH was linearly related to accumulation of lactate plus pyruvate. An increase of 22mumol of lactate plus pyruvate per g of muscle resulted in a fall of 0.5pH unit. The apparent equilibrium constant of the creatine kinase reaction (apparent K(CK)) increased after isometric contraction and a linear relationship between log(apparent K(CK)) and muscle pH was obtained. The low content of creatine phosphate in muscle after contraction as analysed from needle-biopsy samples is believed to be a consequence of an altered equilibrium state of the creatine kinase reaction. This in turn is attributed mainly to a change in intracellular pH.

Adenosine Diphosphate↗

A laterality effect in isometric and isotonic labial tracking.

Hemispheric dominance for sensorimotor control of lip activity was investigated by use of a pursuit auditory tracking task. This task involves continuous frequency matching of a computer-generated target tone and a subject-controlled cursor tone. Thirty right-handed subjects were tested under isometric lip and hand control, and 20 right-handed subjects under isotonic lip control. Subjects tracked 10 1-min trials under each laterality condition--cursor/right ear, target/left ear, and vice versa. In both experiments tracking performance was better when the lip-controlled cursor tone was presented to the right ear (hence direct contralateral route to left hemisphere). A significant (p less than 0.05) cursor/right-ear advantage was found under isometric hand-tracking. Analysis routines examined relative laterality advantages across several time intervals within each 1-min trial. Consistent lateralization scores in favor of cursor/right-ear presentations (REAs) were independent of the time interval measured. For isometric tracking, 58% of subjects having laterality advantages (p less than 0.10) revealed REAs. For isotonic tracking, 71% of subjects revealed REAs. Implications of the latter finding are discussed relative to a left hemisphere mechanism specialized to integrate movement-generated auditory feedback with dynamic kinesthetic information from the articulators.

Adolescent↗

Muscle soreness and serum creatine kinase activity following isometric, eccentric, and concentric exercise.

Serum creatine kinase (CK) activity and subjective ratings of muscle soreness were assessed in 28 college women following three different arm flexion exercise regimens. The subjects were randomly assigned to an eccentric, isometric, or concentric exercise regimen. Each regimen was equated for total work time and work-to-rest ratio. Blood samples for determination of serum CK activity and perceived soreness ratings were obtained prior to and 5, 10, and 25 h following each exercise. Significant increases in perceived soreness ratings were observed for each exercise regimen. The magnitude of the post-exercise increase in perceived soreness was greatest for the eccentric and the isometric exercises with minimal soreness following the concentric exercise. A small but significant increase in serum CK activity was observed following the three exercises (eccentric = 35.8%, concentric = 37.6%, isometric = 34.0%). The post-exercise serum CK increases did not differ significantly among the three regimens. The rise in serum CK activity suggests that muscle damage occurred during all three tasks. However, due to multiple factors which can affect serum CK levels, the increase in serum CK activity may not provide a sensitive indicator of the magnitude of the injury.

Creatine Kinase↗

Isometric cardiac contraction. a possible cause of the disorganized myocardial pattern of idiopathic hypertrophic subaortic stenosis.

The bizarre septal architecture of idiopathic hypertrophic subaortic stenosis (IHSS) may be secondary to a small systolic cavity with late systolic isometric contraction. We examined ventricular muscle for IHSS-type muscle-fiber disarray in infant hearts in which isometric contraction would occur during development-namely, pulmonary or aortic-valve atresia with intact ventricular septum and normal atrio-ventricular valves. Fifteen patients with aortic atresia and 10 with pulmonary atresia were compared to 25 normal controls of matched age and heart weight. Aortic atresia showed disorganization of muscle-fiber alignment of left ventricle, particularly septum, and intramural coronary-artery changes virtually identical to IHSS. Pulmonic atresia had similar right ventricular disarray and vessel changes, again most marked in the septum. Thus, cardiac muscle-cell disorientation similar to IHSS occurs in infant ventricles with outflow-tract obstruction. This IHSS-type myocardial-fiber disarray may result from altered wall stresses related to isometric systolic contraction.

Adult↗

Age dependence of perimacular white blood cell flux during isometric exercise.

PURPOSE: To investigate the age dependence of perimacular white blood cell flux (WBCF) during isometric exercise. METHODS: Fourteen healthy young (age range: 21-29 years; 24 +/- 3 years, mean +/- SD, 12 male and 2 female) and 15 healthy middle-aged (age range: 45-57 years; 53 +/- 4 years, mean +/- SD; 5 male and 10 female) volunteers were studied. Subjects performed isometric handgrip for 10 minutes and squatting for 6 minutes. WBCF was assessed with the blue field entoptic technique, mean arterial pressure (MAP) was measured with an automated oscillometric device, intraocular pressure (IOP) was measured by Goldmann applanation tonometry and ocular perfusion pressure (OPP) was calculated as 2/3 MAP - IOP. RESULTS: Baseline WBCF was significantly higher in young subjects than in middle-aged subjects (191 +/- 28 vs 142 +/- 23; p = 0.001). Isometric handgrip induced a significant increase in WBCF in the middle-aged subjects (23 +/- 24%; p = 0.005), but not in the young subjects. Squatting significantly increased WBCF in both groups (young: 42 +/- 23%; p = 0.004 and middle aged: 51 +/- 27%; p < 0.001). A significant deviation from baseline WBCF was observed when OPP increased by 42 +/- 4% (p = 0.003) and 35 +/- 4% (p < 0.001) for the young and middle-aged subjects, respectively. The OPP-WBCF relationship was not different between the two study groups. CONCLUSION: Altered retinal autoregulation as observed in vascular ocular disease appears to be unrelated to the normal physiological aging process.

Adult↗

An equilibrium-point model for fast, single-joint movement: II. Similarity of single-joint isometric and isotonic descending commands.

The model for isotonic movements introduced in the preceding article in this issue is used to account for isometric contractions. Isotonic movements and isometric contractions are analyzed as consequences of one motor program acting under different peripheral conditions. Differences in isotonic and isometric EMG patterns are analyzed theoretically. Computer simulation of the EMG patterns was performed both with and without the inclusion of possible effects of reciprocal inhibition. A series of experiments was performed to test the model. The subjects made fast isotonic movements that were unexpectedly blocked at the very beginning in some of the trials. The observed differences in the EMG patterns between blocked and unblocked trials corresponded to the model's predictions. The results suggest that these differences are due to the action of a tonic stretch reflex rather than to preprogrammed reactions. The experimental and simulation findings, and also the data from the literature, are discussed in the framework of the model and the dual-strategy hypothesis. They support the hypothesis that the motor control system uses one of a few standardized subprograms, specifying a small number of parameters to match a specific task.

Journal Article↗

Recovery of isometric grip strength after Colles' fracture: a prospective two-year study.

Grip strength during short and sustained maximal voluntary isometric contractions was measured in 28 females and 5 males with displaced Colles' fracture involving the distal radio-ulnar joint. The patients were randomized into two groups, treated either through immobilization with plaster cast or with external fixation. The recovery of isometric grip strength was followed over a two-year period. A significant difference was registered between women with plaster casts and women with external fixators six weeks after the fracture. Regaining of grip strength occurred up to one year after the fracture. The pattern of recovery was slower for women with primary external fixation. Neither the dominant nor the non-dominant injured side regained short or sustained maximal voluntary isometric contraction. The dominant injured side showed no significant difference between sides but the non-dominant injured side remained significantly weaker. It is thus important to identify hand dominance. Pain during measurements was reduced after two years, but about one-fifth of the patients still perceived pain. The present findings may serve as guidance in physiotherapy for these patients.

Aged↗

Maximal isometric force and muscle cross-sectional area of the forearm in fencers.

The maximal isometric force (MIF) of a muscle is directly related to its cross-sectional area (CSA). Strength training produces an increase in muscular force while muscular hypertrophy becomes appreciable at a later time; in asymmetric sports, training causes significant increases in force and muscular mass of the dominant limb of the athlete. The aim of this study was to analyse the differences in muscular force and trophism between the dominant and non-dominant forearms in fencers and in controls. The data of 17 male distance runners (age 21.4 +/- 2.4 years, body mass 74.0 +/- 5.0 kg, height 180 +/- 6 cm) were compared with those of 58 male fencers (age 23.0 +/- 6.7 years, body mass 71.9 +/- 9.3 kg, height 178 +/- 7 cm) drawn from the ranking lists of the National Fencing Committee. They trained for a mean of 11.4 +/- 6.0 (range 2-36) years, commencing at 10.7 +/- 4.5 years of age. Cross-sectional area (muscle plus bone) was estimated in the dominant and non-dominant forearm using a simplified anthropometric method. Maximal isometric force was determined using a mechanical handgrip dynamometer. The differences in CSA and isometric force between the two limbs and between fencers and controls were tested using paired and unpaired Student's t-tests, respectively. Significant differences in CSA and maximal force were observed between the dominant and non-dominant forearm in fencers (both P < 0.001) and in controls (P < 0.005 and P < 0.001, respectively). The fencers showed a greater CSA (P < 0.001) and force (P < 0.001) in the dominant forearm compared with the control group. Furthermore, the differences between the dominant and non-dominant limb of the fencers were significantly greater than the differences between the dominant and non-dominant limb of the controls (P < 0.001 for CSA and P < 0.05 for force). No significant differences in stress ratio (force/CSA) were obtained in either group.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗

Ultrastructure of clots during isometric contraction.

We explored the retraction or contraction of platelet-fibrin clots under isometric conditions. In the presence of micromolar calcium clots of normal platelet-rich plasma developed tension at an initial rate of 0.1 to 0.2 g/min per cm2 (initial cross-sectional area). Electron microscopy of clots fixed after attaining a force of 1.6 g/cm2 revealed platelets with elongated bodies and pseudopods in close apposition to fibrin strands which were oriented in cablelike fashion in the direction of tension. The development of tension could not be explained simply on the basis of platelet-platelet association and interaction alone. First, factor XIII-dependent cross-linking of fibrin fibers was critical to normal isometric contraction. Second, tension decreased linearly, rather than exponentially, when the platelet count in the platelet-fibrin clot was decreased, suggesting that platelets must be interacting with another component (i.e. fibrin). Thrombasthenic platelets, deficient in fibrinogen receptors, failed to develop tension or to align fibrin strands or pseudopods in the clot. Platelet-fibrin clots treated with vincristine to disassemble microtubules or cytochalasin B to disrupt microfilaments failed to develop tension and relaxed if these agents were added after tension had developed. Relaxation under these conditions, however, was not associated with loss of orientation of fibrin strands. Our findings suggest that platelet-fibrin interaction in clots under isometric conditions leads to orientation of fibrin strands and platelets in the direction of force generation. Tension develops as platelets simultaneously attach to and spread along fibrin strands, and contract. The contraction draws some fibrin into platelet-fibrin clumps and aligns other strands in the long axis of tension. The achievement and maintenance of maximum tension appears to depend on the development of platelet-fibrin attachments and extension of platelet bodies and long pseudopods containing bundles of microfilaments and microtubules along the oriented fibrin fibers.

Blood Platelets↗

Structural dynamics of frog muscle during isometric contraction.

Intensity fluctuation autocorrelation functions of laser light scattered by actively contracting muscle were measured at points in the scattered field. They were reproducible and showed characteristics which depended on the physiological state of the muscle and the parameters of the scattering geometry. The autocorrelation functions had large amplitudes and decay rates that varied significantly with the phase of the contraction-relaxation cycle. The dependence of the autocorrelation function on scattering geometry indicated many elements with diameters on the order of 0.5 mum (presumed to be myofibrillar sarcomeres or their A bands or I bands) undergo independent random changes in their axial positions and their internal distribution of optical polarizability during the plateau of an isometric tetanus. The experimental results are interpreted in terms of a model in which most of the scattering elements in isometrically contracting muscle have random fluctuating axial velocities of average magnitude 20 nm/ms that persist for a few milliseconds at least. In addition to these axial motions there are local fluctuations in polarizability. Similar intensity fluctuation autocorrelation functions were observed throughout the active state on two muscle preparations, whole sartorius muscle and small bundles of single fibers (three to eight) of semitendinosus muscle. These results imply that the tension developed during an isometric tetanus contains a fluctuating component as well as a constant component.

Animals↗

The deficit of the isometric tetanic tension redeveloped after a release of frog muscle at a constant velocity.

Frog sartorius muscles tetanized isometrically were released at a constant velocity from lengths lL to lS (delta l = lL -lS; Ls greater than lO). The tension PS redeveloped after the release was lower than the isometric tension PS at LS, and higher than the isometric tension PL at lL. The tension deficit D is defined as the difference PS-PS. The timing of the release during the tetanus did not influence D. D/PO was proportional to delta l/lO. The proportionality constant k was equal to 1.35 +/- 0.19 (n = 8) when the velocity of release was 2.5 mm/s. When the muscles were released the same delta l, D was found to be an exponential decreasing function of the velocity. The tension deficit was also found in experiments performed in the region lS less than lO. The proportionality constant k was smaller, but the influence of the velocity of the release on D was not modified. When the velocity of the release was changed during the release, D changed accordingly, showing that the effects of delta l and V are multiplicative. These facts suggest a working hypothesis based on the concept that the actin filaments which enter the overlap region during a release are strained by the tetanic stress and therefore unable to make normal cross-bridges.

Animals↗

Spontaneous baroreflex sensitivity in young and older people during voluntary and electrically evoked isometric exercise.

BACKGROUND: In young people, cardiovagal baroreflex sensitivity alters during isometric exercise. We investigated whether the reduced resting baroreflex sensitivity seen with increasing age is similarly altered during exercise. METHODS: Cardiovagal baroreflex sensitivity was examined in 8 young (age+/-SEM, 25+/-1.7 years) and 9 older (61+/-3.0 years) subjects, using sequence analysis during voluntary and electrically evoked isometric exercise (at 30% maximum voluntary strength) and during subsequent post-exercise circulatory occlusion. RESULTS: In all phases of both conditions, baroreflex sensitivity was significantly reduced in the older group compared with the young group. (Median (interquartile range), voluntary 7.0 (4.4) vs 3.6 (3.8) ms x mmHg(-1), post-exercise circulatory occlusion 9.0 (8.2) vs 4.6 (4.0) ms x mmHg(-1); electrically evoked 6.6 (10.6) vs 3.2 (3.6) ms x mmHg(-1), post-exercise circulatory occlusion 8.3 (7.7) vs 2.9 (2.2) ms x mmHg(-1), young vs older respectively; P<0.05.) There was a marked rightward shift (resetting) of the baroreflex during exercise with the exception of electrically evoked in the older group. CONCLUSION: Our data demonstrate that the reduction in baroreflex sensitivity in older people is maintained during exercise and during post-exercise circulatory occlusion. Resetting of the baroreflex in the older subjects during moderate voluntary isometric calf exercise is largely the result of central command.

Adult↗

Comparison of the effects of inotropic interventions on isometric tension and shortening in isolated ferret ventricular muscle.

In this study, we have compared the effects of a range of positive and negative inotropic interventions on isometric tension and shortening at low load in isolated ferret papillary muscles. It was found that the majority of interventions produced broadly similar effects on tension and shortening. Positive inotropic interventions which behaved in this way included increasing extracellular calcium and increasing frequency at moderate stimulation rates. Acidosis, the addition of cyanide, and metabolic blockade produced by the addition of iodoacetic acid and cyanide, all produced negative inotropic effects which showed a similar pattern. For all these interventions, it was noted that the fractional effect on tension was 1.5-2 times larger than the fractional effect on shortening. This was attributed to the shape of the tension-length relation for cardiac muscle in different inotropic states. When frequency was increased to a high rate, tension rose to an initial peak, but then showed a marked decline, while shortening rose to a new level and was then well maintained. When protocols consisting of varying amounts of isometric and isotonic contraction were applied, the amount of the secondary decline for both tension and shortening was related to the period spent doing isotonic contractions, and hence to the energy consumption of the muscle. Thus the difference in the behaviour of tension and shortening under these conditions can be accounted for by the lower energy requirements of shortening, rather than by other factors. Over the range of inotropic interventions studied, peak shortening velocity was a more sensitive index of contractility than shortening, being roughly comparable to isometric tension.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗