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At least 811 records · Page 45Linked to original sources
PubMed1986

Isolation of Coxiella burnetii from human sources.

A total of 153 human milk samples were subjected to Capillary Agglutination Test (CAT) for determination of C. burnetii antibodies using phase-I CAT antigen and 22 (14.37%) samples were found to be positive. Twenty two CAT positive milk samples were further processed for the isolation of C. burnetii. Of seven samples positive in first screening, only four revealed presence of C. burnetii by giving specific seroconversion in guineapigs. Similarly, 16 aborted placentas were processed for C. burnetii and three yielded morphological evidence for rickettsial bodies on first screening. However, only one of three samples positive in first screening showed presence of C. burnetii in aborted placentas by specific seroconversion in guineapigs. Isolation of C. burnetii from aborted human placenta is the first report from India.

Agglutination Tests↗
PubMed1990

Injection of inactivated phase I Coxiella burnetii increases non-specific resistance to infection and stimulates lymphokine production in mice.

The encounter of phase I C. burnetii with the host results in seemingly disparate consequences. On the one hand, in vitro lymphocyte responses to mitogens and homologous recall antigen are suppressed. On the other, host resistance to a variety of infectious agents and to a tumor is increased. An explanation for this augmented immune response surely involves the ability of C. burnetii to stimulate cytokines, such as interferon and TNF, which enhance host immune function.

Animals↗
PubMed1990

Prevalence of antibodies to Rickettsia conorii Coxiella burnetii and Rickettsia typhi in Salamanca Province (Spain). Serosurvey in the human population.

The Salamanca Province of Spain is an endemic zone for Mediterranean spotted fever. In this area, only one case of Q fever has been reported and typhus group rickettsial diseases have never been diagnosed. To obtain a panoramic view of the presence of antibodies to the most ubiquitous rickettsial agents, 400 sera specimens from a statistically representative sample of the human population of Salamanca City and its surrounding province were subjected to indirect microimmunofluorescent test for antibodies against Rickettsia conorii, Coxiella burnetti, phase II, and Rickettsia typhi antigens. Titers greater than or equal to 1:40 to R. conorii were found in 73.5% of the sera. Positivity was more common in subjects who reported closer contact with the natural environment and/or with domestic animals. Seropositivity to C. burnetti, phase II, was detected in 50.2% of sera. These positive cases were related to rural environmental factors and to previous contact with animals. The frequency of antibodies increased with age showing a progressive exposure to the rickettsial antigen. The prevalence of antibodies to R. conorii and C. burnetii in the human population of Salamanca Province is higher than that reported from any other geographic zone. The study of antibodies to R. typhi showed that 12.5% of the sera had titers greater than or equal to 1:40. According to our results, seropositivity to this rickettsial antigen cannot be related to any particular group of population nor interpreted totally as cross reactivity with R. conorii. Our data show a wide distribution of R. conorii and C. burnetii antigens in Salamanca Province, and also indicate the presence of R. typhi antigens in this area.

Adolescent↗
PubMed1985

[Trial of an erythrocyte antigenic diagnostic agent for detecting antibodies to Coxiella burnetii].

A new method for the preparation of Q-fever erythrocyte antigenic diagnosticum, adaptable to large-scale production, has been developed, and the diagnosticum thus obtained has been found to be highly specific and sensitive. The combined use of the complement fixation and passive hemagglutination tests enhances the effectiveness of the serological study, as it not only ensures a more complete detection of antibodies to C. burnetii, phase I, in humans and cattle, but also gives more precise indications on the time of infection.

Animals↗
PubMed1991

[An immunoenzyme method for demonstrating Coxiella burnetii antigens].

Materials on the development of an enzyme immunoassay (EIA) system for the detection of the antigens of C. burnetii, the causative agent of Q rickettsiosis, are presented. The system is highly specific and effective with respect to both corpuscular antigens of phases 1 and 2 and soluble antigen (lipopolysaccharide). The sensitivity of this method varies within the range 5-100 ng/ml. The effectiveness of EIA as a quantitative (semiquantitative) control test used in the process of the production of Coxiella preparations has been demonstrated.

Animals↗
PubMed1987

Rickettsial antibody in southern France: antibodies to Rickettsia conorii and Coxiella burnetii among urban, suburban and semi-rural blood donors.

Three hundred and twenty-five sera from blood donors in the south of France were examined by means of the indirect fluorescent antibody test. 18% of the sera had antibodies to Rickettsia conorii, with a significantly higher prevalence (26%) in urban and suburban areas than in semi-rural areas (13 to 16%). This supports the view that in the south of France the highest prevalence of Mediterranean Spotted Fever is suburban. 5% of the sera had antibodies to Coxiella burnetti in this area, in which Q fever is endemic.

Adolescent↗
PubMed1979

Suppression of PHA-stimulated lymphocyte transformation in cynomolgus monkeys following infection with Coxiella burnetii.

Phytohemagglutin (PHA) induced blastogenesis of peripheral blood lymphocytes from cynomolgus monkeys infected with C. burnetii was suppressed between 14 and 28 days after infection. Lymphocytes became responsive to PHA again on day 35 with an increase in stimulation index when cultured with specific antigens. In contrast, production of specific humoral antibodies was not diminished during the acute and early convalescent stages of infection.

Animals↗
PubMed1977

[Rickettsial arteritis due to Coxiella Burnetii].

Case of an 48 year old man who has presented from 1968 to 1973 a lot of diseases such as: --mitral incompletence discovered in 1968 in Madagascar island in spite of many previous clinical examinations; --acute pneumonia and heart failure in January 1973. Serological reactions of Ricketsia were quite positive; --acute thrombosis of right humeral artery in May 1973. It has been treated by surgical way, bay "desobstruction" and by pass and medical treatment chloramphenicol). Pathologic endartery has been inoculated to an hamster, cobaye. These animal became feverish, and presented an inflammation of testis. A least serological reaction of Ricketsia became positive for all of them; --few weeks, thrombosis of left femoral and posterior tibial arteries treated by surgical and medical ways. Some commens are exposed about evolution of Coxiella Burneti infections, about the frequency of arterial and cardiac lesions, and about the effect of tifomycine which seems to be decreasing and the action of cycline (doxicycline).

Agglutination Tests↗
PubMed1989

Red plaque formation of Coxiella burnetii and reduction assay by monoclonal antibodies.

A red plaque technique for C. burnetii which utilizes primary chicken embryo cells, is described. Red plaques could be consistently detected as early as 6 days, usually 8 days post inoculation (p.i.), reflecting that C. burnetii proliferated within the phagolysosomes of host cells. Incubation with phase II monoclonal antibodies or inactivated immune sera containing phase I and phase II antibodies or phase II antibodies only, markedly reduced phase II C. burnetii red plaques. On the other hand, red plaques from phase I organisms increased several times when phase I cells were mixed with phase I monoclonal antibodies or inactivated immune sera containing phase I and phase II antibodies. By indirect red plaque reduction assay red plaque production by phase II cells could be reduced as well.

Animals↗
PubMed1976

Electron microscopic study on the interaction between normal guinea pig peritoneal macrophages and Coxiella burnetii.

An electron microscopic study was conducted to explore the interaction between normal guinea pig peritoneal macrophages and phase I and II Coxeilla burnetii previously treated with either normal or immune serum. A comparison was made on the efficiency of phagocytosis and subsequent killing of rickettsiae by macrophages. Both phases of rickettsiae previously treated with normal serum multiplied within phagosomes after phagocytosis with resultant destruction of macrophages. In contrast, suspending rickettsiae in immune serum rendered them more susceptible to phagocytosis and potentiated their destruction within macrophages.

Animals↗
PubMedDate not supplied

[Susceptibility of Ornithodoros papillipes (Argasidae) ticks to the rickettsia Coxiella burnetii].

A definition of susceptibility of an arthropod to an infectious or invasional agent is given. The susceptibility means a capacity of the arthropod for infection in a result of which the agent either completes a part of its life cycle in the organism of its invertebrate host or reproduces repeatedly in the organs and tissues of its internal medium causing or not causing a noticeable damage. On the model of the tick O. papillipes and Q. fever agent there were shown differences in the resistance of arthropods to Rickettsiae depending on the developmental phase of the invertebrate host. Under otherwise equal conditions the susceptibility of nymphs of O. papillipes to C. burnetii was in nymph II 146 times, in nymph I 280 times and in larvae 50 000 times less than in nymph IV. It has been established that nymphs I, II, and IV engorge blood only 2.5, 14 and 50 times more respectively than larvae. It shows that the success of infection depends to a larger extent on the degree of susceptibility of an arthropod to a corresponding agent than on the quantity of engorged infected blood. The formula of estimation of agent concentration necessary for successful infection of 50 per cent of ticks is given.

Animals↗