PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “Embryo Transfer”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 811 records · Page 45Linked to original sources
PubMed1991

Human serum albumin versus serum: a comparative study on embryo transfer medium.

OBJECTIVE: This study has examined the efficacy of human albumin as a replacement for serum in embryo transfer (ET) medium. DESIGN: In a prospective, randomized study, 224 cycles were analyzed. Patients were randomly divided into three groups: in group A, embryos were transferred in 75% serum; in group B, in 8% serum; and in group C, 2.25% human serum albumin (HSA). SETTING: All the ETs were performed in patients from the in vitro fertilization program of the Center for Reproductive Medicine, Vrije Universiteit Brussel, Belgium. PATIENTS, PARTICIPANTS: In 194 patients, 224 ETs were performed. The patients were selected when they had at least one good-quality embryo. INTERVENTIONS: None. MAIN OUTCOME MEASURE(S): Use of albumin in culture medium resulted in higher pregnancy rates (PRs). To further simplify the procedure, albumin was also used in ET medium. RESULTS: An overall PR of 43% in group A, 27% in group B, and 36% in group C was achieved. No significant difference was observed in PRs between three different transfer media. CONCLUSIONS: We conclude that HSA is a safe and suitable replacement for serum, both in embryo culture and in the transfer medium.

Adult↗
PubMed1986

Differences in cellular immune response to L-glutamic acid60, L-alanine30, L-tyrosine10 (GAT) in full sibling embryo transfer cattle: examination of requirements for cell proliferation.

Pairs of full sibling embryo transfer cattle that expressed identical MHC class I and II products were tested for their in vitro proliferative response to GAT. Peripheral blood mononuclear cells from these cattle were either high or low responders to GAT. Cells from certain pairs of MHC identical siblings gave opposite responses. Low responder animals were further tested to determine if they might respond to GAT with different kinetics, with secondary in vitro restimulation, or with exogenous help provided by interleukin 2. Also, the role of antigen presenting cells and suppressor T cells from low responder animals was investigated. Using appropriate in vitro conditions, cells from all animals tested could respond to GAT. However, MHC identical animals tested under similar conditions exhibited differences in their response to GAT which suggests the proliferative immune response was influenced by factors in addition to MHC coded products.

Animals↗
PubMed2003

Attitudes of IVF/ICSI-twin mothers towards twins and single embryo transfer.

BACKGROUND: Our aim was to study attitudes towards twins and single embryo transfer (SET) among IVF/ICSI-twin mothers with 3 to 4-year old children. METHODS: A national survey was conducted by questionnaire (n = 1769). The study population consisted of IVF/ICSI-twin mothers (n = 266), and the two control groups of IVF/ICSI-singleton mothers (n = 764) and non-IVF/ICSI-twin mothers (n = 739), all giving birth in Denmark in 1997. The overall response rate was 81%. RESULTS: More IVF/ICSI-twin mothers (84.7%) preferred twins as their first child compared with IVF/ICSI-singleton mothers (62.3%) and non-IVF twin mothers (60.0%). The IVF/ICSI-twin mothers had a significantly higher wish for twins (OR = 4.4, 95% CI 2.8-6.9) compared with the non-IVF/ICSI-twin mothers. Though relatively few IVF/ICSI-twin mothers (23.4%) and IVF/ICSI-singleton mothers (17.3%) agreed to SET, delivery of a child with very low birth weight (VLBW) (<1500 g) was predictive of high acceptance of SET (OR = 2.9, 95% CI 1.2-6.9). CONCLUSION: Our study suggests that IVF/ICSI-twin mothers have a greater wish for twins compared with non-IVF/ICSI-twin mothers. Despite the fact that only a quarter of IVF/ICSI mothers agreed to SET, delivery of a child with VLBW and hence high morbidity was predictive of high acceptance of SET. The implementation of elective SET requires extensive counselling of the infertile couples and legislation including strict selection criteria may also be used to facilitate this process.

Adult↗
PubMed1999

The efficacy and adverse effects of in vitro fertilization and embryo transfer.

This paper examines the current status of in vitro fertilization and embryo transfer (IVF-ET) as a treatment for various types of infertility. We reviewed studies on the efficacy and safety of IVF-ET and intracytoplasmic sperm injection (ICSI) plus IVF-ET, compared with conventional treatment or no treatment for various infertility diagnoses. Material retrieved included English language publications between 1992 and January 1997 that reported the results of prospective controlled clinical trials, cohort studies, and retrospective comparative studies with large series, and reviews presenting risks, complications, and longer-term health consequences associated with IVF-ET and ICSI. No adequate prospective comparative studies of sufficient power on the use of IVF-ET for specific infertility diagnoses have been reported to date. Most of the published reports concerning results with IVF-ET as a treatment of infertility have been based upon small, uncontrolled studies, with various methodological weaknesses. Reported results are not directly comparable. There are few follow-up data on outcomes after pregnancy is established or on long-term health consequences of the use of IVF-ET on mothers and their babies. IVF-ET has diffused widely without comprehensive assessment of its efficacy and safety. The available evidence supports its use only for severe bilateral tubal occlusion. For other diagnoses of infertility the evidence is limited and does not establish whether IVF-ET is effective. Long-term, well-designed, prospective clinical trials are required to determine when and for what indications IVF-ET is effective and what its health effects are on both mothers and their babies.

Embryo Transfer↗
PubMed1991

Use of in vitro fertilization and embryo transfer to circumvent infertility caused by an inherited imperforate vagina in mice.

An autosomal recessive mutation (ipv) causing infertility in homozygous females (ipv/ipv) because of imperforate vaginae was discovered in a line of mice selected for low lean tissue mass as a proportion of body weight. The aim of this study was to determine if the mutation could be propagated in offspring following embryo transfer of oocytes collected from mutant females and fertilized in vitro with sperm from males known to carry the gene (ipv/?). Caudal epididymal sperm were incubated with cumulus-enclosed oocytes for 8-10 hr in tissue culture medium 199 + 5% fetal calf serum + 0.4% bovine serum albumin. Oocytes possessing at least two pronuclei were transferred to recipient CD-1 females which had been mated 24 hr earlier to vasectomized males. A total of 683 oocytes was collected from 27 superovulated mutant females. A large proportion of the oocytes was abnormal as evidenced by cytoplasmic fragmentation (259/683, 38%). Seventy-eight percent (331/424) of the normal oocytes were fertilized and 181 of these were transferred to 10 recipients. Six of 10 recipients delivered 38 offspring (24 females, 14 males). Fifty-eight percent (14/24) of the female offspring displayed an imperforate vagina. The results demonstrate that in vitro fertilization and embryo transfer can be used for propagating a mutant gene that causes infertility in females.

Animals↗
PubMed1996

Observations on the transmission of scrapie in experiments using embryo transfer.

This investigation studied the maternal transmission of scrapie in sheep by using embryo transfer to examine the viability of highly susceptible offspring derived from scrapie-affected and uninfected donors. The study also examined the effect of washing the embryos. Scrapie occurred in both washed and unwashed embryo-derived Sip sAsA progeny from both groups of donor ewes. As a result, the earlier observation that scrapie might pass via the unwashed embryo to develop as disease in adult sheep has to be reassessed. Several other implications of the work are considered, including the possibility that natural scrapie is not purely a genetic disease.

Alleles↗
PubMed1990

The perurethral technique for ultrasound directed follicle aspiration in an in-vitro fertilization and embryo transfer programme: a report of 636 patient cycles.

The data from 636 patient cycles from an outpatient-based in-vitro fertilization and embryo transfer programme are presented. Between 1984 and 1988, 545 women (86%) underwent ultrasound directed follicle aspiration (UDFA) exclusively by the perurethral route. The mean follicle aspiration rate was 93%, and the mean number of oocytes per procedure was 5.8. The complication rate was 6%, but none was major. From July 1985 the clinical pregnancy rate per UDFA was 17% and per embryo transfer 21%. The perurethral approach was used in combination with another UDFA route in 91 women (14%) but the need to change route decreased with increasing operator experience. Perurethral UDFA is an efficient and safe method for harvesting oocytes.

Female↗
PubMed2000

Development rates of male bovine nuclear transfer embryos derived from adult and fetal cells.

This study compared the nuclear transfer (NT) embryo development rates of adult and fetal cells within the same genotype. The adult fibroblast cells were obtained from a 21-yr-old Brahman bull. The fetal cells were derived from a Day 40 NT fetus previously cloned using cells from the Brahman bull. Overall, similar numbers of blastocysts developed from both adult (53 of 190; 28%) and fetal (39 of 140; 28%) donor cells. Improved blastocyst development rates were observed when fetal cells were serum-starved (serum-fed 12% vs. serum-starved 43%; P < 0.01) whereas there was no similar benefit when adult cells were serum-starved (both serum-fed and serum-starved 28%). Day 30 pregnancy rates were similar for blastocysts derived from adult (6 of 26; 23%) or fetal (5 of 32; 16%) cells. Day 90 pregnancy rates were 3 of 26 for adult and 0 of 32 for the fetal cell lines. One viable bull calf derived from a 21-yr-old serum-starved adult skin fibroblast was born in August 1999. In summary, somatic NT embryo development rates were similar whether adult or fetal cells, from the same genotype, were used as donor cells. Serum starvation of these adult donor cells did not improve development rates of NT embryos to blastocyst, but when fetal cells were serum-starved, there was a significant increase in development to blastocyst.

Age Factors↗
PubMed1986

Sonography of the endometrium during conception and nonconception cycles of in vitro fertilization and embryo transfer.

The thickness of the endometrium was compared in 15 patients who conceived and 15 who did not with an in vitro fertilization and embryo transfer (IVF-ET) protocol after ovulation induction with human menopausal gonadotropin/human chorionic gonadotropin (hMG/hCG). There was no statistically significant difference (P = 1.0) in the endometrial thickness in the conception versus the nonconception group. Average estradiol (E2) values and number of mature follicles were also not statistically different in the two groups (P = 0.78, P = 0.81). There was a slightly significant difference in the number of embryos transferred in the conception versus nonconception groups (2.5 versus 1.9, P = 0.005). However, the most significant difference between the conception and nonconception groups was the total number of oocytes retrieved (4.4 versus 2.8, P = 0.005). These findings indicate that there are no sonographically detectable differences in the endometrial thickness in patients who achieve pregnancy versus those that do not when given a similar ovulation induction regimen of hMG/hCG for IVF-ET.

Chorionic Gonadotropin↗
PubMedDate not supplied

Cumulative probability of pregnancy following IVF with ICSI and fresh or frozen embryo transfer.

The authors studied the cumulative probability of pregnancy for up to 4 consecutive embryo transfer (ET) cycles with ICSI performed for male factor. Transfers could be either fresh or frozen. The clinical pregnancy rate (PR) for the first 4 cycles were similar [44% (61/366); 31% (44/138); 45% (14/31); 44% (4/9)]. Delivery rates were also similar. There was a lower PR on the second retrieval vs. the first retrieval (47% vs. 29%), but this may be related to most of the second retrievals occurring in the second transfer cycle (67%, 31/55); this may be explained by women who were poor responders and required another retrieval without a frozen ET. The majority of transfers in cycle 1 were fresh, whereas cycles 2-4 used primarily frozen-thawed embryos. These data should be helpful for patients requiring IVF with ICSI in deciding to continue with more IVF cycles or consider other

Cryopreservation↗
PubMedDate not supplied

Embryo transfer and related technologies in sheep reproduction.

This paper reviews the status of embryo transfer and the major technologies applied to preimplantation of embryos in sheep. Embryo production from superovulated ewes is hindered by an unpredictable response to hormonal treatment. Progress in this area should be expected by an appropriated control of follicular development with gonadotropin-releasing hormone (GnRH) agonist or antagonist prior to gonadotrophin administration. Simple protocols for the cryopreservation of sheep embryos by vitrification are already available and the development of frozen-thawed blastocysts to term is close to the fresh ones. Further research is required to identify factors able to promote the maturation in vitro of oocytes, namely those obtained from prepubertal animals. Semen and embryo sexing procedures are available in cattle although much less attention was paid to their application to sheep. Among all the reproductive technologies, cloning with embryonic and foetal cells has progressed dramatically in sheep and nuclear transfer has been used to produce transgenic animals as an alternative to pronuclear injection. The production of the first lamb cloned from a somatic cell opened new opportunities in animal breeding as well as exciting lines of basic research. The overall conclusions are that, apart from superovulation, the application of in vitro technologies is likely to evolve rapidly and once applied, a great impact on traditional and new animal productions should be expected. However, a better understanding of the changes in gene expression, induced in embryos by different in vitro manipulation procedures, is necessary to prevent abnormal foetal development.

Animals↗
PubMed2000

Influence of female age on pregnancy outcome in in vitro fertilization and embryo transfer patients undergoing intracytoplasmic sperm injection.

PURPOSE: To determine the influence of female age on the outcomes of ICSI in IVF-ET patients. METHODS: One hundred and seventy-five couples underwent 352 cycles of ICSI. The quality of oocytes and embryos, fertilization rate, and pregnancy outcomes were retrospectively evaluated according to female age; < 30 years in Group A (49 cycles), 30-34 in Group B (177 cycles), 35-39 in Group C (97 cycles), and > or = 40 in Group D (29 cycles). RESULTS: The fertilization rates were not significantly different among the age groups. Significant negative linear correlations were observed between female age and the numbers of oocytes retrieved and embryos transferred, and cumulative embryo score. Clinical pregnancy rates were significantly decreased and spontaneous abortion rate increased with advancing age. CONCLUSIONS: Female age may be a prognostic indicator in ICSI program.

Adult↗
PubMed1995

The impact of different types of anesthesia on in vitro fertilization-embryo transfer treatment outcome.

OBJECTIVE: Our objective was to evaluate retrospectively the influence of different types of anesthesia on the outcome of ovum retrieval. METHODS: Sedation combined with local anesthesia was used on 120 occasions (Group I), epidural block in 139 ovum retrievals (Group II), and general anesthesia in 173 cycles (Group III). RESULTS: No differences were found in embryo yield or number or the quality of the embryo transferred. Of 99 pregnancies achieved, 66 live deliveries were recorded. Significantly lower clinical pregnancy rates were found in Group III (14.5%) compared with Group II (23.7%; P = 0.018) or Group I (25.8%; P = 0.0074). Highly significant differences were found in the delivery rates between Group III (8.7%), Group II (20.11%; P = 0.0017), and Group I (19.2%; P = 0.0043). CONCLUSION: The use of general anesthesia, especially nitrous oxide, for oocyte retrieval has an adverse effect on IVF outcome. This deleterious effect manifests itself only after embryo transfer and leads to lower pregnancy and delivery rates.

Adult↗
PubMed1998

Modulation of blood pressure in the Dahl SS/jr rat by embryo transfer.

Gestational hypertension and malnutrition are associated with hypertension and ischemic heart disease in the adult human. The impact of the gestational environment on the adult blood pressure in two well-characterized genetically homogeneous rat strains, the hypertensive SS/jr and normotensive SR/jr, was studied by cross-fostering within 6 hours of birth and by embryo transplantation with the recipient dam nursing the transplanted pups. Systolic blood pressure (BP) was measured by tail-cuff plethysmography twice a week after the age of 7 weeks. The lactational environment (cross-fostering) had no effect on blood pressure. Embryo transfer between like strains had no effect on the development of hypertension, nor did the BP of R transferred to S (RetS) differ from that of normal R or RetR. At 7 weeks of age, the BP of SetR was significantly lower than that of S or SetS (P<.01) and was similar to that of RetR and R. With age, the blood pressures of the S, SetS and SetR increased at approximately the same rate but from a significantly different baseline. Salt-sensitivity in the S and resistance in the R were not altered. The protective effect of the R gestational environment on SetR female BP was abrogated during whelping and lactation. Embryo transfer and cross-fostering did not alter the weight of rats older than 7 weeks. Because the BP of the R dams were significantly lower than that of the S dams, these studies do not distinguish between the effects of the R dams' lower blood pressure per se and hormonal influences of the R uterus on the S blood pressure phenotype.

Adult↗
PubMed1995

Pituitary down-regulation prior to in-vitro fertilization and embryo transfer: a comparison between a single dose of Zoladex depot and multiple daily doses of Suprefact.

In order to further evaluate the endocrinological, embryological and clinical efficacy of a single injection of the gonadotrophin-releasing hormone (GnRH) analogue Zoladex (goserelin), 142 women underwent pituitary down-regulation prior to in-vitro fertilization and embryo transfer: 71 with a single injection of Zoladex depot (group I) and 71 matched controls with multiple daily injections of Suprefact (buserelin; group II). Ovarian stimulation was performed with human menopausal gonadotrophin (HMG) and ovulation induction with human chorionic gonadotrophin (HCG). HMG and hydroxyprogesterone caproate depot were given for luteal phase support. The mean (+/- SD) age (34.01 +/- 4.42 versus 34.81 +/- 4.00 years), mean total dosage of HMG (61.25 +/- 26.87 versus 56.17 +/- 25.18 ampoules), mean daily dosage of HMG (4.74 versus 4.94 ampoules), duration of HMG stimulation (12.91 +/- 3.68 versus 11.31 +/- 3.46 days) and oestradiol concentration on the day of HCG (10,082 +/- 8007 versus 9440 +/- 7840 pmol/l) were similar in both groups but the mean total number of injections (GnRH and HMG) (13.55 +/- 3.35 versus 55.37 +/- 31.92) was significantly lower in group I. Furthermore, the proportion of women down-regulated by 2 weeks and pregnancy rate per embryo transfer were significantly higher in the Zoladex group, while miscarriage rates were similar. We conclude that a single dose of Zoladex is quicker, more convenient and should be investigated as an equally effective alternative to multiple doses of Suprefact for pituitary down-regulation prior to assisted conception. Further studies are required to test the teratogenicity and effectiveness of Zoladex.

Adult↗
PubMed1996

A prospective, randomized study of embryo transfer results after 3 or 5 days of embryo culture in in vitro fertilization.

OBJECTIVES: To compare the implantation rate of embryos after 3 and 5 days of IVF culture. DESIGN: Prospective randomization of ET depending on the weekday of ovum pick-up (OPU). SETTING: University Department of Endocrinology and Reproduction. PATIENTS: All women entering an outclinic IVF program. INTERVENTIONS: Two hundred thirty-three ETs performed on day 3 after OPU and 410 performed on day 5 were analyzed. When blastocysts with a clear inner cell mass were available, a maximum of two were replaced. RESULTS: On day 3 after OPU, 60 pregnancies per 233 ET (26%) and on day 5, 102 pregnancies per 410 ET (25%) were induced. The average implantation rate per embryo was 13% and 12%, respectively. After subdivision according to embryo morphology, pregnancy rate per ET (n = 59) and implantation rate per embryo on day 3 with exclusively unfragmented embryos were 32% and 18%, respectively, not significantly different from ET (n = 73) exclusively with embryos containing > 0% and < 20% fragments: 27% and 12%. After transfer on day 5, when one or more cavitating embryos were available (n = 227), pregnancy and implantation rates were 40% and 23%, statistically different from ET on day 3. On day 5, ET exclusively with morula stages showing signs of starting blastulation (n = 26), pregnancy rate and implantation rate were 12% and 11%, respectively, from ET (n = 157) with embryos not reaching the latter stage: 6% and 3%. CONCLUSIONS: Overall ET results after 3 and 5 days are comparable. After 5 days of culture, one to two embryos can be replaced with an average implantation rate of > 23% per embryo, minimizing the incidence of triplets.

Blastocyst↗
PubMed2001

Embryo transfer after autologous endometrial coculture improves pregnancy rates.

Since the first in-vitro fertilization (IVF) baby born in 1978, the technique of in-vitro fertilization-embryo transfer (IVF-ET) has been well established and widely used by clinicians all over the world. However, the success rate of IVF-ET still remains relatively low. Sub-optimal embryo culture condition may consider as a factor contribute to the poor success rate. Autologous endometrial coculture is a newly developed technique able to enhance development of fertilized eggs using the patient's own endometrial cells. A clinical tryout was conducted in our institute. Patients underwent either classic IVF (n = 82) (i.e., control group) or autologous endometrial coculture (n = 134) (i.e., coculture group). The clinical pregnancy rate (per patient) was significantly higher in coculture group (48.5%) than control group (24.3%). Patients were further subgrouped according to their age. For each subgroup, the clinical pregnancy rates were again consistently higher and the miscarriage rates were consistently lower in the coculture group than in the control groups. Coculture also increased clinical pregnancy rate of patients who failed IVF at least twice from 0% to 26.6%. Our data confirmed that autologous endometrial coculture is an effective method to improve the success rate of IVF-ET. The evidence of improving clinical pregnancy rates and reducing abortion rates after coculture suggest that coculture indeed improved success rates by improving embryo quality.

Adult↗