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At least 811 records · Page 45Linked to original sources

PubMed1993

Developmental profile of the gene expression of a Bombyx homolog of mammalian sorbitol dehydrogenase during embryogenesis in non-diapause eggs.

1. By using semi-quantitative PCR analysis, changes in the amount of the transcript for a Bombyx homolog of mammalian sorbitol dehydrogenase (BmSDH) were examined during embryogenesis in non-diapause eggs. 2. Occurrence of the transcript for BmSDH correlated with the two developmental phases, the growth of embryo and the formation of larval tissues. 3. In the first phase, an increase in the amount of the transcript for BmSDH resulted from embryonic cells rather than yolk cells. 4. In the second phase, the transcript was suggested to be abundant in fat-body cells of pharate larva, because it was abundant in fat-bodies of 5th instar larvae. 5. The difference in the expression of BmSDH gene between diapause and non-diapause eggs is discussed.

Animals↗

PubMed1987

Tissue-specific expression of the mouse Q10 H-2 class-I gene during embryogenesis.

We have studied the pattern of expression of the Q10 gene, a H-2 class-I gene located in the major histocompatibility complex which encodes a soluble class-I molecule, in the mid-gestation mouse embryo, and compared it to those of two other class-I genes, namely Kd and 37, the latter gene located in the thymus leukemia region. We found that the steady-state amount of these different mRNAs gradually increased from day 13 to day 18. By comparison with the level of expression of these genes in adult liver, the increase during gestation was fairly more marked for Q10 mRNA than for the others. Furthermore, we found that the Q10 gene is transiently expressed in the endoderm layer of the visceral yolk sac and in the fetal heart. Expression in the latter tissue decreases abruptly while increasing in the liver. It has been proposed that the Q10 protein is involved in immune tolerance. However, the time course of expression of Q10 mRNA and its tissue distribution during embryogenesis suggest that the Q10 protein could play a role in the differentiation of hematopoietic stem cells.

Animals↗

PubMed1994

Hel-N2: a novel isoform of Hel-N1 which is conserved in rat neural tissue and produced in early embryogenesis.

Hel-N1 is a neural-specific RNA-binding protein which is highly conserved over evolution. The data presented here demonstrate alternative 5' splicing of Hel-N1 mRNA, characterized by the insertion of a novel 91-bp exon. The resultant isoform, Hel-N2, has a potentially expanded N-terminal region of 29 amino acids when compared to Hel-N1. Reverse transcription (RT)-PCR cloning data indicate that homologues (Rel-N1 and Rel-N2) exist in rat neural tissue where there is 96% conservation at the nucleotide level. RT-PCR analysis of rat embryonic tissue RNA indicates that this splicing event occurs as early as day 12 of embryogenesis. The temporal pattern of expression of Rel-N1 and Rel-N2 resembles that of the Drosophila melanogaster homologue, elav, and supports the prior notion that these mammalian counterparts participate in early neural development.

Alternative Splicing↗

PubMed1995

The Drosophila melanogaster homologue of the human BBC1 gene is highly expressed during embryogenesis.

We have previously reported the isolation and preliminary characterisation of a full-length cDNA sequence derived from the human BBC1 gene, a gene which displays differential expression in tumours of the female breast [Adams et al., Hum. Mol. Genet. 1 (1992) 91-96]. Here, we report the isolation and characterisation of the Drosophila melanogaster (Dm) homologue of this human gene. The Dmbbc1 cDNA is 62% identical to the human BBC1 cDNA within a conserved open reading frame and the encoded proteins share 74% sequence similarity. The Dmbbc1 mRNA is expressed at all stages of Dm development, with the highest levels of expression occurring during embryogenesis. In addition, the Dm and human BBC1 proteins share remarkable degrees of identity with the products of recently isolated plant and avian bbc1 cDNAs. The sequences of all the predicted BBC1 proteins are highly similar to that of the rat ribosomal subunit protein L13 [Olvera and Wool, Biochem. Biophys. Res. Commun. 201 (1994) 102-107], strongly indicating that the BBC1 protein is ribosomal protein L13.

Amino Acid Sequence↗

PubMed1993

Activity-dependent development of the neuromuscular synapse during Drosophila embryogenesis.

In Drosophila, mutations in specific ion channel genes can increase or decrease the level of neural/synaptic activity. We have used these genetic tools, in combination with classical pharmacological agents, to modulate neural activity during embryogenesis and examined effects on the differentiation of an identified neuromuscular junction. We find that electrical activity is required for the neural induction of transmitter receptor expression during synaptogenesis. Likewise, neural electrical activity is required to localize transmitter receptors to the synaptic site. In muscles with activity-blocked synapses, a low level of receptors is expressed homogeneously in the muscle membrane as in muscles developing without innervation. Thus, presynaptic electrical activity is required to mediate the neural induction of the transmitter receptor field in the postsynaptic membrane.

Animals↗

PubMed1988

Gene expression in amphibian embryogenesis.

The study of molecular events during the embryogenesis of Xenopus laevis has advanced as a result of the availability of molecular markers, i.e., nucleic acid and antibody probes for genes that are expressed in a temporally and spatially regulated fashion during development. In this article we summarize results on the localized expression of keratin genes and on the reconstruction of regulated transcription of the gastrula/neurula-specific DG42 gene. Furthermore, we discuss experiments that investigate molecular events during mesoderm induction and provide information on the nature of the inducing principle.

Animals↗

PubMed1990

Histone variant patterns during vertebrate embryogenesis and limb development.

Two-dimensional gel electrophoresis was used to examine the relative content of core histone variants during early chicken embryogenesis and at selected stages of hindlimb development. Nuclei from stage 19 limb buds displayed a pattern similar to whole embryos at stage 1, at which time all of the known avian histone variants, including the minor isoprotein H3.3, were detected. Variant ratios did not change during limb development, up to stage 29. However, the portion of H2A variants migrating as ubiquitinated conjugates increased more than twofold during limb development, advancing from 4.5% of the total H2A proteins at stage 19 to 12% at stage 29.

Animals↗

PubMed1990

Collagen type IV of Drosophila is stockpiled in the growing oocyte and differentially located during early stages of embryogenesis.

We have developed and characterized a battery of specific polyclonal antibodies directed against specific portions of the alpha-chain of collagen type IV synthesized in Drosophila by the gene DCg1. Here, we describe the use of these antibodies together with in situ hybridization experiments in an attempt to study the expression and localization of collagen type IV during Drosophila oogenesis and early embryogenesis. The results clearly demonstrate that DCg1 is maternally expressed by follicle cells and that the collagen type IV chain produced is stockpiled in the growing oocyte. During the gastrulation stages, this component of Drosophila basement membranes concentrated on cells involved in the gradual invaginations leading to morphogenetic furrows. The presence of collagen type IV, which is an RGD-bearing molecule, during early stages of Drosophila development is discussed in comparison with the crucial, active role its vertebrate counterpart is supposed to play in morphogenetic processes.

Animals↗

PubMed1991

Segmentally restricted, cephalic expression of a leucine zipper gene during Drosophila embryogenesis.

The expression pattern and DNA sequence of a newly identified gene, CNC (cap'n'collar), suggest a role for this gene in cephalic patterning during Drosophila embryogenesis. In situ hybridization reveals transcripts localized to the mandibular segment and the hypopharyngeal and labral primordia first detectable in late blastoderm stages. Sequence analysis of cDNA clones from the CNC locus shows the CNC gene product to be related to transcription factors of the leucine zipper (bZIP) class. Based on its protein sequence, we propose that CNC is a subunit of a heterodimeric regulatory protein involved in the control of head morphogenesis.

Amino Acid Sequence↗

PubMed1991

The mouse homeobox gene, S8, is expressed during embryogenesis predominantly in mesenchyme.

The murine S8 gene, originally identified by Kongsuwan et al. [EMBO J. 7(1988)2131-2138] encodes a homeodomain which resembles those of the paired family. We studied the expression pattern during mid-gestation embryogenesis of S8 by in situ hybridization. Expression was detected locally in craniofacial mesenchyme, in the limb, the heart and the somites and sclerotomes all along the axis, and was absent from the central and peripheral nervous system, splanchnopleure, and endodermal derivatives. This pattern differs considerably from that of most previously described homeobox containing genes. By genetic analysis, the gene was located on chromosome 2, about 20 cM from the HOX-4 cluster.

Amino Acid Sequence↗

PubMed1993

A zebrafish homologue of the Drosophila neurogenic gene Notch and its pattern of transcription during early embryogenesis.

We describe here the primary structure of a zebrafish homologue of the Drosophila neurogenic gene Notch and its pattern of mRNA accumulation during embryogenesis. The gene produces a 8.5 kb transcript encoding a putative transmembrane protein with a high degree of sequence similarity to members of the Notch family, comprising 36 EGF-like repeats, three lin-12/Notch repeats, six cdc10/SWI6 repeats, OPA repeats and a PEST sequence. Transcription of the zebrafish Notch gene is spatially and temporally regulated. A high density of transcripts, most probably of maternal origin, can already be detected in the 2-cell stage. During pregastrulation stages, RNA is present in all cells. However, following gastrulation, transcripts accumulate in specific regions of the embryo following a rapidly changing pattern. In some of these regions, cell divisions take place at the time of Notch expression, in others processes of cell differentiation. This holds true for various mesodermal derivatives, such as the prospective notochord, and for different neural primordia, such as the neural plate and the brain vesicles. This pattern of transcript accumulation suggests a role for the zebrafish Notch homologue in processes of regionalization and cell diversification.

Amino Acid Sequence↗

PubMed1993

The Polycomb gene is differentially regulated during oogenesis and embryogenesis of Drosophila melanogaster.

Homeotic genes are responsible for determining the identity of body structures along the anterior-posterior axis. In Drosophila the early patterning system defines the differential expression pattern of the homeotic genes. In laterstages the Polycomb-group (Pc-G) genes were found to keep homeotic genes stably repressed in those domains where they have to be inactive. At the molecular level the Pc-G is supposed to exert its repressory role by influencing the higher order structure of chromatin. Here we show that during oogenesis the Polycomb (Pc) protein is localized in the polytene nuclei of the nurse cells. In addition, in late stages we observe overlapping gradients of expression in the somatic follicle cells, suggesting also an important function of Pc on the determinants involved in egg formation. During embryogenesis Pc is found in all tissues, though in later stages it preferentially accumulates in the CNS. Interestingly, we have identified a feedback-type regulation: the Ultrabithorax gene, a homeotic target gene of Pc, in its own domain of expression is down-regulating Pc.

Animals↗

PubMed1994

Characterization of the mouse HNF-4 gene and its expression during mouse embryogenesis.

Hepatocyte nuclear factor 4 (HNF-4) is a member of the nuclear receptor gene superfamily with unknown ligand. It has been assumed to play an important role in the regulation of gene expression in the liver. Here, we report the cloning and characterization of the mouse HNF-4 gene, as well as its expression during embryogenesis. The HNF-4 protein is encoded by ten exons. The gene structure is unique in the steroid receptor superfamily in that the second zinc finger is encoded by two exons. HNF-4 mRNA is expressed in a limited number of mouse adult tissues: liver, kidney, intestine, stomach and skin. HNF-4 could play an important role in the formation and function of visceral yolk sac and in the development of the liver and kidney since its mRNA, as determined by in situ hybridization, appears upon primary differentiation of these organs. As a first step in the study of the regulatory elements of the HNF-4 gene, we mapped the transcription start site and carried out DNase I hypersensitive site (HS) analysis over a region of approximately 22kb upstream of the gene. The complexity of the HSs suggests that multiple elements might contribute to the transcriptional regulation of the HNF-4 gene.

Aging↗

PubMed1994

Characterisation of T48, a target of homeotic gene regulation in Drosophila embryogenesis.

In Drosophila, the homeotic genes encode transcription factors which specify segmental identities during embryogenesis by directly regulating subordinate target genes. A chromatin immunopurification approach has been used to identify targets of the homeotic gene Ultrabithorax (Ubx). We report here the characterisation of a candidate target gene which is positively regulated by the homeotic genes Ubx and abdominal-A in both the epidermis and the midgut visceral mesoderm and encodes a novel transmembrane protein. By mapping the cis-regulatory regions of this gene, we have identified elements responsible for mediating homeotic regulation, and show that they contain in vitro Ubx binding sites. Therefore, we suggest that this is an example of a direct target of regulation by homeotic genes in two germlayers.

Amino Acid Sequence↗

PubMed1994

A beta 1-integrin associated alpha-chain is differentially expressed during Xenopus embryogenesis.

The antigen of mAb 2F10 was identified as a Xenopus beta 1-integrin associated alpha-chain by the criteria (1) that it coprecipitates with anti beta 1-antibody, (2) that it changes molecular mass upon reduction in a way that is characteristic for integrin alpha-chains and (3) that it is present on cell membranes. This alpha-chain, termed alpha 2F10, is found in small amounts in the pregastrula stages of Xenopus development and accumulates thereafter in the embryo, alpha 2F10 can be detected by immunofluorescence first at stage 17 of embryogenesis on the cell membranes of the sensorial layer of the ectoderm, the notochord and the endoderm. This characteristic pattern of distribution is maintained throughout the following embryonic stages. Timed explanation experiments indicate that all cells of the pregastrula have the potency to express alpha 2F10. This potency becomes successively restricted during gastrulation to yield the ultimate pattern of expression.

Animals↗

PubMed1994

Xenopus laevis cellular retinoic acid-binding protein: temporal and spatial expression pattern during early embryogenesis.

There is increasing evidence that retinoic acid (RA) has a role in establishing normal axial patterns during Xenopus laevis embryo-genesis. Several types of retinoid binding proteins are thought to mediate the effects of RA. We report the isolation of a cDNA, named xCRABP-b, which encodes a X. laevis cellular retinoic acid-binding protein (xCRABP). This cDNA hybridises to a transcript in gastrular stage embryos of approximately 3 kb, much larger than those CRABP transcripts expressed in mice. The expression of the xCRABP mRNA is generally restricted to tissues which are sensitive to the teratogenic effects of excess RA. It is likely, that during normal X. laevis embryogenesis, concentrations of RA in RA-responsive cells are modulated by the xCRABP gene product.

Amino Acid Sequence↗

PubMed1995

A role for intermediate filaments in the establishment of the primitive epithelia during mammalian embryogenesis.

Investigations of the cytoskeleton in mammalian eggs and embryos have revealed the existence of an unusual array of crosslinked intermediate filaments composed of cytokeratins 5, 6, 16, and 'Z' that are referred to as cytoskeletal sheets. We have been investigating the function of these cytoskeletal sheets during embryogenesis. In this investigation we report the rapid appearance of extensive arrays of tonofilaments extending across blastomeres and in association with intercellular desmosomal junctions appearing at the time the embryo hatches from its zona pellucida, through the time of implantation of the embryo into the uterine wall. Just prior to the time of gastrulation these tonofilaments disappear. Electron microscopy and immunoconfocal microscopy demonstrate that the tonofilaments are composed of cytokeratins characteristic of the type found earlier in development, that is types 5 and 6; whereas, cytokeratin type 8 which has been shown to be synthesized in blastocysts is localized primarily at perinuclear regions. Cytokeratins 8 and 18 are synthesized to about the same extent as actin at the time the tonofilaments appear whereas the synthesis of cytokeratins 5 and 6 is greatly reduced. Our results suggest that cytokeratins 5 and 6 in the tonofilaments may arise from the stored form of cytokeratins in the cytoskeletal sheets. Consequently, our results suggest that the sheets may serve as a maternal reserve of cytokeratin employed by the embryo at the time of implantation to form extensive arrays of tonofilaments in the embryo that likely provide structural integrity to the embryo as it is subjected to mechanical stress during invasion and implantation into the uterine wall.

Animals↗

PubMed1996

AP-2.2, a novel gene related to AP-2, is expressed in the forebrain, limbs and face during mouse embryogenesis.

Using a differential subtractive hybridization cloning procedure we have recently identified the AP-2.2 gene as a novel early retinoic acid-induced gene in murine P19 embryonal carcinoma cells. We have also shown that the AP-2.2 protein, which is highly related to the AP-2 transcription factor, can activate transcription when bound to an AP-2 consensus binding site [Oulad-Abdelghani et al. (1995) Mol. Cell. Biol., submitted]. We report here the in situ hybridization pattern of expression of AP-2.2 transcripts during mouse embryogenesis. At 7.5 days post-coitum, AP-2.2 transcripts were detected in the boundary region between neural plate and surface ectoderm, as well as in extra-embryonic tissues. By 8.0-8.5 gestational days, AP-2.2 transcripts appeared to be expressed in premigratory and migrating neural crest cells. Over the following days, the AP-2.2 gene displayed region-restricted expression in the facial mesenchyme, especially around the embryonic mouth cavity and the nasal cavities, as well as in the surface ectoderm, nasal and oral epithelia. AP-2.2 RNA was also specifically expressed in the presumptive cortical region of the forebrain vesicles. AP-2.2 transcripts were restricted to the distal mitotic area (the 'progress zone') of the limb buds and of the genital bud. AP-2.2 expression also appeared to be specific for primordial germ cells in the genital ridges. Thus, the AP-2.2 gene is expressed in several embryonic areas whose development can be affected by retinoids, such as the forebrain, face and limb buds.

Animals↗