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Relative repellency of woodsmoke and topical applications of plant products against mosquitoes.

The repellent action of various plant products was evaluated against anopheline and culicine mosquitoes in a rural village in the Wosera area, Papua New Guinea. A 5 x 5 Latin square design was used. Wood from four home-grown plant species was burned outdoors in the first experiment, and mosquitoes attracted to human bait were collected from 1800 to 2400 hours. In the second experiment, bruised leaves from another four plant species were rubbed on to the legs of human baits followed by mosquito collections. Woodsmoke and topical applications reduced biting of human volunteers by 79% and 51%, respectively. This low-technology control method may be included in the range of options for householders in order to reduce mosquito nuisance and improve their standard of health.

Administration, Cutaneous↗

[Individual protection against insect vectors].

Many diseases for which no vaccine is available are transmitted by insect and arthropod vectors, the main exceptions being yellow fever and Japanese encephalitis B. Treatment is less and less effective due to the development of chemoresistance to therapeutic and prophylactic drugs as is well-illustrated by malaria. One of the best methods of preventing these diseases is personal protection against insect bites. Personal protection measures can be divided into three categories which can be used separately or in combination : application of repellents to the skin, wearing clothes impregnated with insecticides, and use of bed nets and other barriers impregnated with insecticides. The choice of method depends on the type of insect vector involved. For insects that are active during the day or at dusk, application of repellents to the skin gives good short-term protection and wearing impregnated clothes is useful. Bed nets that have been properly impregnated with pyrethroids are highly effective for night-time protection. Since personal protection methods are not 100% effective, they must be used in association with chemoprophylaxis according to medical guidelines. Medical advice should be sought if fever should occur especially after returning from a trip in the tropics.

Animals↗

Insect matters.

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Animals↗

Studies on the transmission potential of filariasis in controlled areas of Henan Province.

OBJECTIVE: To study the regular pattern of growth and declination or the transmission potential of filariasis after the disease was basically (the microfilarial rate was lower than 1%) in Henan Province in 1987. METHODS: According to the distribution of filaria species and original microfilarial rate, in 7 surveillance sites in 7 counties (cities) the etiology and mosquito vector surveys were carried out continuously during 1988-1995 and no control measurement for pathogen was taken. RESULTS: Ten residual microfilaremias became negative gradually in the first 6 years and no new microfilaremias occurred during 1988-1995. During 1993 to 1995, the microfilaremias rate of population in the sites was 0. The natural infection rates of filarial larvae in vector mosquito were 0.1%-0. During the 8 years, 15 vector mosquitos were positive with a total of 18 filarial larvae which were all of first or second-stage larvae. The C. pipiens pallens was the main vector, the second was A. sinensis, with a small number of C. fatigans and A. anthropophagus. The man-biting rates of mosquitoes for outdoor sleepers fluctuated greatly, the highest one was 360.6 mosquitoes/person per night and the lowest, 7.2. The man-biting rates of mosquitoes for sleepers inside mosquito-net was about 1 mosquito/person per night. The proportion of multiparous mosquitoes also fluctuated more greatly, the highest one was 88.1% and the lowest 27. 2% According to the data described above, the man-biting rate of vector mosquito which contained filarial L3 was 0. CONCLUSIONS: The results suggested that after the microfilarial rate was lower than 1%, the residual microfilaremias became negative gradually in 3-6 years, and the transmission of the disease was blocked. Therefore, in the areas where filariasis was basically controlled, elimination of the disease was attainable within sight.

Animals↗

Effect of skin abrasions on the efficacy of the repellent deet against Aedes aegypti.

Abrasion of repellent-treated human skin affected the efficacy of a sustained-release insect repellent containing N,N-diethyl-3-methylbenzamide (deet) against bites of Aedes aegypti. Skin treated with repellent when abraded up to 30 times showed significantly lower protection than unabraded skin against mosquito bites for 10 h. The mean value of the kinetic coefficient of friction during skin abrasion by clothing (battle dress uniform fabric) for repellent-treated skin (0.159 +/- 0.003) was significantly higher than untreated skin (0.122 +/- 0.005). Repellent-treated skin appeared stickier than the untreated skin. An increase in the number of skin abrasions by clothing resulted in a reduced duration of protection against mosquito bites.

Aedes↗

Field efficacy of a new repellent, KBR 3023, against Aedes albopictus (SKUSE) and Culex quinquefasciatus (SAY) in a tropical environment.

Two new repellent formulations, KBR 3023 10% and 20% from Bayer AG, Germany, were evaluated together with DEET 10% and 20% as standard repellent formulations. Evaluation was based on two separate field studies: a daytime study (0900-1700 hr) in a forested orchard on Penang Island and a nighttime study (2100-0100 hr) in a squatter residential area on the adjacent mainland of peninsular Malaysia. Both studies were carried out by exposing humans with bare arms and legs to mosquitoes landing/biting for an eight hour period. Right arms and legs of the human baits were treated with different repellent formulations (KBR 3023 10%, 20% and DEET 10%, 20%) and the left limbs were left untreated to act as controls. The daytime study indicated that all four formulations were equally effective (P < 0.05) as repellents against the predominant Aedes albopictus with greater than 88.5% reduction in landing/biting in the first four hours and not less than 65.0% in the next four hours of the assessment period. In the night study, all four formulations were also found to be equally effective (P < 0.05) in repelling Culex quinquefasciatus, the predominant species. All four formulations provided complete protection against Cx. quinquefasciatus in the first two hours of exposure. The percentage reduction values were maintained above 90.0% for the next six hours of the assessment period. In conclusion, both the KBR 3023 and DEET formulations were found to be equally effective (P < 0.05) in providing a long-lasting reduction in human-mosquito contact in both the day and night field studies.

Aedes↗

[A study on the transmission of plague though seven kinds of fleas in rat type and wild rodent type plague foci in Yunnan].

In order to assess vector position and vector action of seven kinds of fleas in the rat type and wild rodent type plague foci in Yunnan, we studied the indices as: infection rate, rate of feed bacterial emboli, colony transmission vector efficiency, life-span, bloodthirsty to human, body weight, quantity of blood-sucking, rate of dissociation by using animal model and raise film method. The results showed that Xenopsylla cheopis and Neopsylla specialis specialis were the main vectors of two type plague foci. Pulex irritans was an important vector of human plague in Yunnan. This paper reported that Xenopsylla cheopis and Neopsylla special special could twice form bacterial emboli, and with other information about vector efficiency, Index of Pulex irritans, Neopsylla special special, Frontopsylla spadix, Monopsyllus anisus, rate of bloodthirsty to human and life-span of seven kinds of fleas. Reason from quiescence to resuscitate was also deduced and the vector position of Leptopsylla segnis was revised. The vector action of main vector for Ctenophalmus quadratus was negated which proved that Apodemus chevrieri was the main reservoirs in these plague foci.

Animals↗

Mosquito bite hypersensitivity.

We report a patient who experienced systemic anaphylaxis after several mosquito bites. The skin tests were positive, by prick and intradermal tests, with two species of common mosquito (Aedes communis and Culex pipiens) and also with red midge larvae (Chironomus). Specific IgE against Aedes communis was demonstrated. We carried out SDS-PAGE and immunoblotting with whole body extracts of Aedes communis and Culex pipiens and with red midge larvae. The immunoblotting results showed specific IgE against two proteins of approximately 30 kDa with the extract of Culex pipiens. We also found IgE against one protein of a slightly lower molecular weight with Aedes communis, and against several proteins (the most important with a molecular weight between 30 and 70 kDa) with the Chironomus extract. Cross-reactivity between these insect species was suspected.

Adult↗

Studies on biting density and biting cycle of Culex quinquefasciatus, say in Khon Kaen City, Thailand.

Biting density and biting cycle of Culex quinquefasciatus in Khon Kaen City were examined during November 1994 to October 1995. Biting activity of the mosquito was assessed by using the indoor human bait method. Trapped mosquitos were identified and carefully dissected to determine their parities. Climatory data ie temperature, relative humidity and rainfall were also recorded during the study. The densities of mosquito ranged from 1.6/man/hour in December to 9.2/man/hours in March with the average of 5.0/man/hours. The lowest biting density was observed in winter and higher densities were in summer and rainy seasons. The temperature was the most important variable that influenced the biting density in each month of the year (p<0.05). The biting cycle showed that the mosquito active throughout the night, with peak activity at 22.00-23.00 hours. Parous rate of the mosquito ranged from 33.3% in February to 71.9% in November with the average of 47.3%. The biting proportion of parous mosquitos was high during the early hours of the night and gradually decreased until 06.00 hours, whereas that of the nulliparous mosquitos showed an increasing trend from 18.00 to 06.00 hours. The biting cycle of the parous mosquito reached the peak activity at 21.00-22.00 hours, 1 hour ahead of the peak for nulliparous mosquito. These findings suggested that Cx. quinquefasciatus in Khon Kaen City may be able to transmit bancroftian filariasis if they were exposed to microfilaria carrier individuals.

Animals↗

Bites and stings of Hymenoptera, caterpillar and beetle.

Insects compose a very large group of creatures that bite or sting but only a few are truly venomous in the sense that they inject a venom into human victims. Anaphylaxis from a Hymenoptera sting is the most serious reaction from this group and is estimated to account for approximately half of the annual fatalities from venomous bites and stings in the United States. Epinephrine, Benadryl, steroids, hyposensitization, local tissue management and symptomatic and supportive care are discussed.

Adult↗