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Teflon buttress inhibits recanalization of uncut stapled bowel.

The uncut Roux limb operation is designed to have the benefits of a Roux limb but still have electrical continuity from proximal to distal bowel, thus eliminating the risk of Roux stasis syndrome. The main complication has been recanalization of the uncut staple line leading to bile reflux. This study aims to employ a new technique, which will not allow recanalization of an uncut staple line but will not interfere with normal bowel myoelectric activity. Fourteen mongrel dogs, 25 to 35 kg, underwent a midline laparotomy under general anesthesia. An uncut staple line was placed 25 cm from the ligament of Treitz. In seven animals an uncut staple line alone was placed, and in the other seven animals the bowel was stapled between a sandwich of Teflon reinforcing strips such that the staples were held on both sides of the bowel by the Teflon. A jejunojejunostomy was placed 6 cm proximal to the staple line. Insulated bipolar electrical leads were placed around the staple line. After the electrical leads were monitored 2 days to 3 months postoperatively for bowel myoelectric activity, The animals were killed and the operative sites inspected. No animal suffered morbidity or mortality from the procedure. All seven unreinforced staple lines recanalized and all seven reinforced staple lines remained competent. The duodenal pacemaker potentials were transmitted through the staple line in five animals (3 controls and 2 with Teflon reinforcement) with in 1 week postoperatively. The uncut staple line does not reliably transmit the duodenal pacemaker potentials. The staple line does not recanalize when it is reinforced with a permanent material, increasing the utility of the "uncut" Roux limb operation.

Action Potentials↗

Surgical treatment of Roux stasis syndrome.

We wondered whether the slow gastric emptying of the Roux stasis syndrome could be improved by performing a corrective "uncut" Roux operation. Five dogs had a standard Roux gastrectomy and placement of serosal electrodes on the proximal jejunum and Roux limb. After recovery, baseline myoelectrical and gastric emptying data were collected. The animals then underwent a second operation: take down of the Roux limb, restoration of jejunal continuity, and construction of an "uncut" Roux limb. After the animals recovered, the tests were repeated. The slow frequency of pacesetter potentials (PPs) in the standard Roux limb (mean +/- standard error of the mean 14 +/- 0.4 cpm) was unchanged after the uncut Roux operation (14 +/- 0.5 cpm, P > 0.05). However, a greater percentage of PPs propagated aborally in the uncut Roux limb (81% +/- 4%) than in the standard Roux limb (53% +/- 7%, P <0.05). Nonetheless, gastric emptying of a 250 ml 10% dextrose liquid meal was not speeded by the uncut Roux operation (uncut Roux = 36% +/- 5% emptied by 20 minutes vs. standard Roux = 35% +/- 7%; P >0.05). Bile acid concentrations in gastric aspirates were minimal after both operations (0.7 +/- 0.2 micromol/L vs. 0.6 +/- 0.1 micromol/L; P >0.05). The conclusion was that more PPs propagated in the aborad direction in the uncut Roux limb than in the standard Roux limb, but gastric emptying was not speeded by the uncut Roux operation. Both operations were equally effective in preventing bile reflux into the gastric remnant.

Anastomosis, Roux-en-Y↗

Restoration of myoelectrical propagation across a jejunal transection using microsurgical anastomosis.

The aim of this study was to determine whether microsurgical anastomosis can restore propagation of jejunal pacesetter potentials (PPs) across a site of canine jejunal transection and preserve motility and transit in bowel distal to the transection. A complete jejunal transection with exact microsurgical anastomosis was performed in five dogs, while five dogs with intact jejunum and five dogs with complete transection and end-to-end conventional macrosurgical anastomosis were used as controls. Long-term recording electrodes and intraluminal, open-tipped pressure catheters were implanted in all dogs. The mean frequency of PPs decreased distal to the transection in both groups of transected dogs. However, aborad propagation of PPs across the anastomosis occurred episodically by 3 months in each dog that had a microsurgical anastomosis, but never occurred in any dog with a conventional macroanastomosis. Moreover, the motility and transit in bowel distal to the transection were unaltered in the dogs with a microsurgical anastomosis, whereas they decreased in the dogs with a macroanastomosis. The conclusion was that microsurgical anastomosis of transected canine jejunum restored episodic propagation of PPs across the anastomosis, and preserved motility and maintained transit in bowel distal to the anastomosis. The conventional macroanastomosis did none of these.

Anastomosis, Surgical↗

Rumination documented by using combined multichannel intraluminal impedance and manometry.

According to the Rome criteria, rumination is considered to be an esophageal functional disorder. Because documentation of rumination is difficult, it has been suggested that rumination is a clinical diagnosis. Even though a clinical diagnosis of rumination can be based only on history, this conclusion often leaves both the patient and the physician dissatisfied. In this article we describe the case of a 26-year-old man referred for evaluation of persistent regurgitation. By using combined multichannel intraluminal impedance and manometry we were able to document the sequence of events leading to regurgitation. These include an increase in intra-abdominal pressure identified in the pressure transducer located in the stomach, which initiates the reflux event identified by both impedance changes as well as common cavity phenomenon, followed by peristaltic fore contractions, which clear the intraesophageal content, a pattern highly suggestive for rumination. The opportunity to review these documented changes visually helps to make the patient aware of the functional aspects of the condition, avoiding unwarranted therapies and providing the opportunity to reinforce behavioral modifications.

Adolescent↗

Distension-induced myoelectrical dysrhythmia and effect of intestinal pacing in dogs.

The aims of this study were to investigate the effect of duodenal distension on intestinal myoelectrical activity and to investigate whether intestinal pacing was able to reverse the effects of distension. Six female hound dogs with four pairs of electrodes on the proximal jejunum were involved in this study. The protocol consisted of 30 min of recording of jejunal myoelectrical activity as baseline and 90 min of recording during distension. Intestinal pacing was performed during the second 30 min of distension. Duodenal distension severely impaired intestinal myoelectrical activity. The percentage of normal slow waves was reduced from 90.8+/-8.4% at baseline to 73.8+/-10.2%, 57.2+/-11.4%, and 53.7+/-16.0% during the first, second and third 30 min of distension (P<0.05, ANOVA). The dominant power was similarly decreased and the minute-by-minute variation of dominant frequency was significantly increased after distension. Intestinal pacing reversed distension-induced dysrhythmia. The percentage of normal slow waves during the 30 min of distension with pacing was significantly higher than the corresponding 30 min of distension without pacing (88.5+/-6.6% vs. 57.2+/-11.4%, P<0.03). It was concluded that intestinal pacing can normalize distension-induced dysrhythmia and has a potential as a future therapeutic modality for intestinal motor disorders.

Animals↗

Significance of autonomic nervous system activity in functional dyspepsia.

The purpose of this study was to examine if patients with functional dyspepsia could be separated into meaningful groups based on their autonomic function. Subjects were divided into two groups, and symptoms, gastric myoelectrical activity, gastric emptying, and psychological factors were compared. Group 1 had less autonomic variability but more cardiac reactivity than group 2. Symptom reports did not differ between groups. Group 1 had higher neuroticism scores than group 2, while group 2 showed greater tachyarrhythmia in response to drinking water than group 1. The relatively low autonomic variability in group 1 is consistent with higher sympathetic activity and may be associated with the group's greater neuroticism. The relative lack of cardiac reactivity in group 2 is consistent with lack of autonomic flexibility and may be related to the tachyarrhythmia observed in that group. The results of this study suggest that autonomic function may play a significant role in functional dyspepsia.

Adult↗

Impairment of gastric and jejunal myoelectrical activity during rectal distension in dogs.

It is known that distension of the rectum induces gastric hypomotility and delays gastric emptying. Its effect on gastrointestinal myoelectrical activity has not been well studied, however. The aim of this study was to investigate the effects of rectal distension on gastrointestinal myoelectrical activity in dogs. Six hound dogs implanted with electrodes on the serosa of the stomach and proximal jejunum were studied. The protocol consisted of a 30-min baseline recording and another 30-min recording during rectal distension. Gastric myoelectrical activity was severely impaired by rectal distension. The dominant power was significantly decreased from -2.79 +/- 0.52 dB at baseline to -4.84 +/- 1.26 dB during distension (P < 0.05). The percentage of normal 4-6 cycles per minute gastric slow waves was reduced from 95.08 +/- 1.11% to 83.63 +/- 4.00% (P < 0.02), and the percentage of tachygastria was increased during distension (0.33 +/- 0.19% vs 6.03 +/- 1.27%, P < 0.02). The instability coefficient of the dominant frequency was significantly increased (0.134 +/- 0.012 vs 0.326 +/- 0.074, P < 0.05). The percentage of slow wave coupling was reduced from 93.99 +/- 0.76% to 73.43 +/- 2.07% (P < 0.00003). In the small bowel, only the instability coefficient of dominant frequency showed a significant increase during distension. Other parameters were not affected by rectal distension. We conclude that rectal distension severely impairs gastric myoelectrical activity. The induced gastric dysrhythmia and reduced slow wave amplitude and coupling may be the underlying pathophysiology of gastric hypomotility and delayed gastric emptying observed during rectal distension.

Animals↗

Impaired postprandial gastric myoelectrical activity in Chinese patients with nonulcer dyspepsia.

Using a homemade electrogastrography (EGG) system, we studied the characteristics of the myoelectrical rhythm in Chinese patients with nonulcer dyspepsia (NUD). Based on short-term Fourier transformation, recorded slow waves could be automatically analyzed to obtain the following parameters: dominant frequency/power, percent of normal frequency (2-4 cpm), power ratio, etc. EGG parameters, Helicobacter pylori status, histological examination of gastric mucosa, and dyspeptic symptoms were recorded in 27 NUD patients. Compared to 32 healthy controls, the Chinese NUD patients had abnormal postprandial EGG parameters including a lower percentage of regular 2-4 cpm slow waves (70.10 +/- 2.97% vs 79.08 +/- 2.95%, P < 0.05), a lower level of increment of dominant power (0.62, +/- 0.91 vs 3.76 +/- 0.58 dB, P < 0.05), lower power ratio (1.42 +/- 0.28 vs 2.79 +/- 0.39, P < 0.05) and a higher instability coefficient (0.36 +/- 0.03 vs 0.26 +/- 0.03, P < 0.05). However, Helicobacter pylori infection and its associated gastritis did not influence any EGG parameters in NUD patients. Six main dyspeptic symptoms and total symptom score had no correlation with any EGG parameters. In conclusion, Chinese NUD patients may have abnormal postprandial stomach myoelectrical activity, but these EGG abnormalities are not a direct result of Helicobacter pylori infection and its related gastritis and do not contribution to the dyspeptic symptoms.

Adult↗

Postprandial improvement of gastric dysrhythmias in patients with type II diabetes: identification of responders and nonresponders.

Using the technique known as electrogastrography, we studied the postprandial response of gastric myoelectrial activity in subjects with type II diabetes. Seventy-one subjects with type II diabetes underwent 1 hr of fasting electrogastrography recording. HbA1c and fasting serum glucose levels were obtained. Subjects then underwent an additional 2 hr of electrogastrography recording in the post prandial state. Sixty of the 71 patients (85%) had gastric rhythm abnormalities in the fasting state. Forty-six of 71 subjects (65%) responded to the test meal by improving their electrogastrography tracings (responders) while 35% did not respond (nonresponders). The time spent in bradygastria during the fasting state by responders was 26.3+/-12.8% vs 10.9+/-8.5% for nonresponders (P < 0.0001). The percent tachygastria during the fasting state in responders was 19.8+/-13.0%, which was less than nonresponders (38.3+/-29.7%) (P < 0.001). Fasting plasma glucose and HbA1c could not be used to predict the gastric myoelectrical response to meal. In conclusion, gastric rhythm disturbances are common in type II diabetes; there was no correlation between HbA1c levels, age, duration of diabetes, or fasting serum glucose and gastric dysrhythmia in response to meal; two groups of subjects emerged: those who became less dysrhythmic in the post pradial state (responders) and those who did not (non-responders); and fasting bradygastria was associated with responders and fasting tachygastria was associated with nonresponders.

Diabetes Mellitus, Type 2↗

Is there a one-to-one correlation between gastric emptying of liquids and gastric myoelectrical or motor activity in dogs?

The aim of this study was to investigate the correlation of gastric emptying with gastric myoelectrical activity recorded from internal serosal electrodes and with gastric motility measured from strain gauges. The study was performed in eight healthy dogs chronically implanted with four pairs of electrodes and two strain gauges on the gastric serosa and equipped with a duodenal fistula for the assessment of gastric emptying. Each dog was fed four liquid test meals on four separate days with identical calories (320 kcal) and volume (473 ml). A correlation was found between gastric emptying and the energy of contraction in the frequency band of 3.75-7.50 cycles/min during the first 30 min after the meal (r = 0.46, P < 0.05). While none of the parameters of gastric myoelectrical activity was correlated with gastric emptying, two major parameters, percentage of regular gastric slow waves and percentage of slow-wave coupling, were found to be associated with delayed gastric emptying. A significant correlation was found between the frequency of gastric slow waves and that of the contractions in both fasting (r = 0.83, P < 0.001) and fed state (r = 0.70, P < 0.001 at 30 min, r = 0.86, P < 0.001 at 60 min). It was concluded that gastric emptying is correlated with the strength of gastric contractions in a frequency range identical to that of the gastric slow waves, and there is no one-to-one correlation between gastric emptying of liquids and any parameters of gastric myoelectrical activity. However, major parameters of gastric myoelectrical activity are associated with gastric emptying.

Animals↗

Characterization of gastric myoelectrical rhythms in patients with systemic sclerosis using multichannel surface electrogastrography.

The aim of this study was to characterize multichannel surface electrogastrography (EGG) recordings in patients with systemic sclerosis (scleroderma, SSc) compared to normal controls. Ten SSc patients and 13 healthy age-matched controls were enrolled in this study. Gastric myoelectrical activity was recorded using a four-channel electrogastrograph with abdominal surface electrodes. The EGG was recorded in the morning for 1 hr in the fasting state, and 1 hr after a standardized (500 kcal) test meal. It was found that: (1) The regularity of the gastric slow wave was significantly lower in the SSc patients when compared with the normal controls in both fasting and fed states. (2) There was a significantly higher incidence of bradygastria in the SSc patients. (3) The SSc patients showed a significantly lower percentage of slow wave coupling among the four-channel EGGs than the controls. (4) In comparison with the controls, the patients showed an impaired spatial distribution of gastric slow wave power in both fasting and fed states and an impaired spatial distribution of slow wave frequency in the fasting state. It was concluded that SSc patients have an abnormal gastric slow wave as shown in the multichannel EGG as a decreased percentage of normal slow waves and impaired spatial coordination of gastric slow waves. The multichannel EGG may serve as a simple, noninvasive, and cost-effective method to assess gastric motility disorders and their relevance in patients with SSc.

Adult↗

Changes in gastric myoelectric activity during space flight.

The purpose of the present study was to examine postprandial myoelectric activity of the stomach and gastric activity associated with space motion sickness using electrogastrography. Three crewmembers participated in this investigation. Preflight, subjects exhibited normal postprandial responses to the ingestion of a meal. Inflight, crewmembers exhibited an abnormal decrease in the power of the normal gastric slow wave after eating on flight day 1, but had a normal postprandial response by flight day 3. Prior to and during episodes of nausea and vomiting, the electrical activity of the stomach became dysrhythmic with 60-80% of the spectral power in the bradygastric and tachygastric frequency ranges. These findings indicate that gastric motility may be decreased during the first few days of space flight. In addition, changes in the frequency of the gastric slow wave associated with space motion sickness symptoms are consistent with those reported for laboratory-induced motion sickness.

Eating↗

Distal small bowel hormones: correlation with fasting antroduodenal motility and gastric emptying.

The aim of the present study was to study the interdigestive motor complex (MMC), distal small intestinal hormones, and gastric emptying in normal-weight and obese subjects before and after jejunoileal bypass (JIB). Therefore, fasting antroduodenal motility, gastric emptying, and RIA for motilin (MOT), neurotensin (NT), peptide YY (PYY), and glucagon-like peptide-1 (GLP-1) was performed in nine obese subjects before (BMI 42 +/- 4 kg/m2) and nine months after (BMI 31 +/- 4) JIB, and in two groups of nine age- and sex-matched controls (BMI 23 +/- 1 and 21 +/- 1). The rate of gastric emptying was faster in obese subjects and GLP-1 lower compared to normal-weight controls. After JIB, fewer phase III of the MMC were observed; fasting levels of PYY were elevated during the MMC; postprandial levels of NT, PYY, and GLP-1 were elevated; and gastric emptying was delayed. Our results suggest that there may be an association between an impaired GLP-1 response after food intake and obesity, and after JIB, PYY seems to regulate interdigestive motility while GLP-1 may regulate early gastric emptying.

Adolescent↗

Edrophonium provocative testing during electrogastrography (EGG): effects on dyspeptic symptoms and the EGG.

The aim of this study was to determine whether edrophonium induces dyspeptic symptoms and/or gastric myoelectric changes in normal subjects and patients with functional dyspepsia. Eighteen normal controls and 28 patients with functional dyspepsia underwent electrogastrography (EGG) with edrophonium administration. After EGG recording with 1-hr fasting and 2-hr postprandial periods, subjects received an intravenous infusion of saline (placebo) followed by edrophonium 10 mg. After each injection, the EGG was recorded for 15 min and symptoms scored. Patients with functional dyspepsia had significantly more frequent gastrointestinal symptoms in response to edrophonium than normal controls. Edrophonium had no effect on EGG dominant frequency, but increased the power of the dominant frequency in both controls and dyspeptic patients. In the dyspeptic patients, reproduction of the patient's symptoms was associated with an increase in the power of the dominant frequency, whereas when no symptoms were produced, there was no change in power. Overall, 21 of 28 dyspeptic patients (75%) had either an abnormal baseline EGG (N=10) or a normal EGG and positive edrophonium test (N=11). In conclusion, edrophonium administration can reproduce symptoms in some dyspeptic patients. Symptoms after edrophonium administration may be related to either more vigorous gastric contractions and/or increased visceral perception of normal gastric contractions.

Adolescent↗

Impaired postprandial gastric slow waves in patients with functional dyspepsia.

The aim of this study was to investigate gastric myoelectrical activity in patients with functional dyspepsia. Thirteen healthy subjects and 14 patients with functional dyspepsia participated in the study. The electrogastrogram (EGG) recording was made in each subject for 30 min in the fasting state and 120 min after a standard test meal of 475 calories. Spectral analysis methods were applied to derive quantitative EGG parameters. There was no difference in the EGG between the patients and controls in the fasting state. However, abnormalities in the postprandial EGG were found in the patients. The percentage of 2-4 cpm waves was significantly lower (74.4+/-4.0% vs 85.7+/-1.6%, P < 0.03) and the postprandial increase in EGG dominant power was significantly less (-0.52+/-0.92 dB vs 2.24+/-0.88 dB, P < 0.03) in patients than in controls. It was also found that the percentage of postprandial 2-4 cpm waves could be used to differentiate the patients with functional dyspepsia from the healthy controls with a specificity of 100% and a sensitivity of 43%. It was concluded that a subset of patients with functional dyspepsia have impaired gastric myoelectrical activity in the fed state.

Adult↗

Chronic intestinal pseudoobstruction associated with fetal alcohol syndrome.

Alcohol acts as a teratogen in the fetus, resulting in prenatal or postnatal growth failure, characteristic facial dysmorphic features, and central nervous system dysfunction. The toxic effects of alcohol on the developing brain are well recognized, but gastrointestinal neuropathy has not been described in fetal alcohol syndrome (FAS). Five children with FAS presented in infancy with signs and symptoms suggestive of chronic intestinal pseudoobstruction. They were not able to sustain adequate caloric intake by mouth, and all required prolonged special methods of alimentation. We performed antroduodenal manometry in these children to determine whether their symptoms were associated with a gastrointestinal motility disorder. All patients had abnormally propagating phase III-like episodes during fasting (retrograde in four, simultaneous in two). Persistent clusters of stationary contractions were a prominent feature in two patients. In utero neurotoxicity of alcohol may not be limited to the central nervous system, but may also cause an enteric neuropathy presenting in infancy as chronic intestinal pseudoobstruction.

Child↗

Effect of local injection of botulinum toxin on sphincter of Oddi cyclic motility in dogs.

To study effects of intrasphincteric injections of botulinum toxin on the sphincter of Oddi cyclic motility and responses to motilin and cholecystokinin, four conscious dogs with duodenal cannula underwent manometry of the common bile duct, sphincter of Oddi, and duodenum. After baseline recording, each dog had intrasphincteric injections of saline or botulinum toxin. The injections of saline had no effect, whereas botulinum toxin significantly reduced mean basal pressure, amplitude, and motility index of the sphincter of Oddi. These effects took place in four to seven days and reached a maximum in seven to 10 days. The basal pressure returned to the baseline level in 28 weeks, but the amplitude and motility index remained low. The pressure parameters of motilin-induced premature phase III-like activity also decreased, but action of cholecystokinin was not affected. These results indicate that the botulinum toxin injections reduce sphincter of Oddi phasic contractile activity for a prolonged period of time.

Animals↗

Human small bowel motor activity in response to liquid meals of different caloric value and different chemical composition.

Previous animal studies have shown that the nature and duration of postprandial motility in the small bowel depend both on the caloric load and the chemical composition of a meal. It is not clear whether this is also true for the human small bowel. Therefore we investigated the motor activity of the human small bowel in response to nutrient liquids of different caloric value and different chemical composition. Ten human volunteers underwent three separate, 24-hr ambulatory manometry studies. They drank water, a pure glucose solution, and Intralipid 10% in volumes of both 300 and 600 ml. The caloric value of the nutrient liquids was 330 and 660 kcal, respectively. Records were analyzed visually for the reappearance of phase III of the MMC after ingestion of a test liquid, and a validated computer program calculated the incidence and amplitude of contractions during the postprandial period. Neither duration of the postprandial interval nor the mean incidence or mean amplitude of contractions were different between the fat and the carbohydrate solutions, but phase III reappeared significantly later after ingestion of the nutrient liquids than after water (P = 0.0002). Duration of the postprandial interval also depended on the volume or the caloric load of a liquid meal (P = 0.0012). Mean incidence of contractions tended to be higher after ingestion of nutrient liquids than after water (P = 0.059). We conclude that in ambulant subjects, small bowel motor activity in response to chemically diverse liquid meals is remarkably uniform. This is true for the duration of the postprandial motor activity, as well as the incidence and amplitude of contractions during that period. The caloric value of a liquid meal, however, regulates the duration of the postprandial interval in the human small bowel.

Energy Intake↗