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At least 811 records · Page 45Linked to original sources

A dyspnea evaluation protocol for respiratory therapists: a feasibility study.

PURPOSE: We tested the feasibility of incorporating a dyspnea evaluation protocol into bedside assessments routinely performed by respiratory therapists (RTs) on mechanically ventilated patients at a university teaching hospital. METHODS: A dyspnea assessment protocol was incorporated into the RT assessments performed at 4-hour intervals on endotracheally intubated, mechanically ventilated patients in our medical and surgical intensive care units. RTs were asked to inquire of all responsive patients: "Are you feeling short of breath right now?" and, if yes, "Is your shortness of breath mild, moderate, or severe?" We analyzed 324 consecutive patient ventilator flow sheets from 77 medical and 161 surgical intensive care unit patients. RESULTS: Dyspnea scores were recorded during 1,870 of 2,539 scheduled RT patient assessments. The protocol compliance rate was 74%. Patients were sufficiently responsive to answer the protocol questions during 32.1% of the bedside assessments. Dyspnea was recorded in 11% (67/600) of those encounters. Dyspnea was described most often as mild. CONCLUSIONS: Initial implementation of a dyspnea evaluation protocol was moderately successful in prompting RTs to ask mechanically ventilated patients whether they felt short of breath during scheduled bedside visits. A rapid bedside evaluation for dyspnea may prove useful in evaluating the effect on patient distress of implementing protocols designed to optimize ventilator settings or the use of sedating drugs during mechanical ventilation. By this approach RTs may also be able to promote a patient-centered approach to managing respiratory failure in the intensive care unit.

Dyspnea↗

[The effect of a treatment protocol on the prognosis of equine laminitis].

All horses and ponies with laminitis treated at the Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, between 1995 and 1998 were analysed retrospectively (n = 152). Based on the results of this study and on the world-wide knowledge on laminitis a standardised treatment protocol was adopted in 1999. Fifty-eight patients have been treated for laminitis between 1999 and 2001 following the treatment protocol. Compared to our hospital-population (orthopaedic patients), mares and ponies are at higher risk developing laminitis, but the prognosis does not differ between sexes as between breeds. Radiological evidence of rotation of the third phalanx seems to be of minor importance for the prognosis. Distal displacement of the third phalanx, however, seems to worsen the prognosis. The outcome of the acute cases is significantly better in the group treated following the protocol, than in the group without protocol. The outcome of the chronic cases is not influenced by the treatment protocol. Although the cases in both groups differ, as they are all clinical cases, our data suggest that the standardised treatment protocol results in a better outcome for cases of acute laminitis.

Acute Disease↗

[The expression of integrin beta3 in mice's endometrium during the implantation window based on different ovarian stimulation protocols].

OBJECTIVE: To compare the uterine receptivity with different ovarian stimulation protocols, the endometrial expression of Integrin beta3 in mice's during the implantation window underwent different ovarian stimulation were studied. METHODS: Forty mice were randomly allocated into 4 groups of 10 mice. (1) GnRHant group, gonadotropin-releasing hormone antagonist (GnRHant) was given first for desensitizing the pituitary, then the pregnant mare's serum gonadotrophin (PMSG) was added for ovarian stimulation. (2) gonadotropin-releasing hormone agonist (GnRHa) group, GnRHa was given first for desensitizing the pituitary, then PMSG was added for ovarian stimulation. (3) PMSG group, injected with PMSG only; and (4) control group, given with saline of the same volume. Human chorionic gonadotropin (HCG) was injected to the mice of the GnRHant, GnRHa, and PMSG groups. Forty-eight hours after the mice of the control group the mice were killed. Their uteri were taken. RT-polymerase chain reaction (RT-PCR) was used to detect the expression of integrin beta3 mRNA. Immunohistochemistry (SP method) was used to locate and semiquantitatively measure the integrin beta3 in the endometrium during implantation window. RESULTS: (1) Immunohistochemistry: showed that the staining intensity of integrin beta3 was 3.74 +/- 0.15 in GnRHa group, 3.22 +/- 0.19 in the GnRHant group, and 3.24 +/- 0.18 in the PMSG group, all significantly lower than that in the control group (3.90 +/- 0.11, P = 0.023, 0.001, and 0.001). with a significant difference between the GnRHa group and the PMSG group (P = 0.001) and without a significant difference between the. GnRHant group and PMSG groups (P = 0.768). (2) RT-PCR showed that the relative quantity of integrin beta3 mRNA expression was 1.14 +/- 0.16 in the GnRHa group, 0.76 +/- 0.33 in the GnRHant group, and 0.73 +/- 0.26 in the PMSG group, all significantly lower than that in the control group (1.4 +/- 0.3, P = 0.045, 0.001, and 0.001). with a significant difference between the GnRHant and PMSG groups (P = 0.001) and without a significant difference between the GnRHant and PMSG groups (P = 0.857). CONCLUSION: All of the ovarian stimulation protocols do harm to the mice's uterine receptivity. Ovarian stimulation combining GnRHa protocol in mice is better than PMSG alone protocol, because it can improve the uterine receptivity. Ovarian stimulation combining GnRHant protocol in mice decrease the uterine receptivity as well as the PMSG alone protocol.

Animals↗

Cryopreservation of shoot apices of in-vitro grown gentian plants: comparison of vitrification and encapsulation-vitrification protocols.

Using vitrification and encapsulation-vitrification protocols, we successfully cryopreserved shoot apices from in-vitro plants of different Gentiana cultivars (lines). Although both protocols gave high survival percentages after storage in liquid nitrogen, the encapsulation-vitrification protocol had several distinct advantages over the vitrification protocol: (i) survival was higher under optimal conditions, (ii) the range of optimal exposure periods to the plant vitrification solution 2 (PVS2) was broader, and (iii) regrowth of cryopreserved shoot apices was apparently more vigorous and faster. Shoot apices from ten cultivars/lines of three Gentiana species (G. scabra, G. triflora, and G. pneumonanthe) were successfully cryopreserved using the two protocols with average survival of 49.0 percent and 73.7 percent for vitrification and encapsulation-vitrification, respectively. These results indicate that the two protocols optimized in the present study are promising for cryopreservation of a wide range of Gentiana genetic resources.

Cryopreservation↗

Determination of rifampicin bioequivalence in a three-drug FDC by WHO and indian protocols: effect of sampling schedule and size.

SETTING: To promote the quality assurance of fixed-dose combination (FDC) formulations, the World Health Organization (WHO) has prepared a convenient simplified protocol for the determination of rifampicin (RMP) bioequivalence. During the development of this protocol, it was proved that sampling time up to 8 h can determine the rate and extent of RMP absorption. However, this protocol utilises 20 volunteers in contrast to other local regulatory requirements of a minimum of 12 volunteers. The different sample sizes utilised in these protocols may affect the sensitivity of the bioequivalence outcome. OBJECTIVE: To determine the effect of sampling size and schedule on RMP bioequivalence when two different protocols are used. DESIGN: A bioequivalence trial was conducted with a study design of 20 volunteers and 24 h sampling time, which fulfils the requirements of both the WHO and Indian regulatory protocols. Pharmacokinetic and statistical analysis was done by stepwise reduction in sample size and schedule. RESULT: Bioequivalence limits of RMP were unaffected by a reduced sample size of 12 volunteers and 8 h sampling time. CONCLUSION: Minimising sample size after validation for borderline and poor quality FDC formulations can further reduce the cost of conducting bioequivalence trials.

Absorption↗

[Comparison of three FOBT protocols for colorectal cancer screening in Chinese--a multicenter study].

OBJECTIVE: To evaluate three FOBT protocols of colorectal cancer screening. METHODS: A multycenter study was conducted based on a consulted program. The effectiveness/cost of each protocole (CFOBT, IFOBT, SFOBT) were evaluated by the identifying patients who underwent CFOBT and IFOBT for 3 times consecutively as well as colonoscopy. RESULTS: 323 eligible patients including 49 colorectal cancers, 60 colon adenomas, 60 chronic colitis, 15 hemorrhoids and 139 normal colon were observed. The effect/cost of CRC screening protocols was following: 1). For 3 times of FOBT consecutively: There was no significant difference for their sensitivity among CFOBT, IFOBT and SFOBT (95.9%, 95.9% & 93.9%) but the specificity of IFOBT and SFOBT (89.21% and 94.24%) were higher significantly than CFOBT (75.54%). The specificity of SFOBT was higher than IFOBT. After adjusting their detecting number of cancer to same level the cost of SFOBT was the lowest. among these FOBTs.2). For 2 times of FOBT consecutively: The sensitivity of CFOBT, IFOBT and SFOBT were 77.8%, 87.8% and 75.5% respectively. The sensitivity of IFOBT was higher than SFOBT and CFOBT significantly. Their specificity were 88.5%, 96.4% and 98.6% respectively. The specificity of IFOBT and SFOBT were higher significantly than CFOBT. However, there was no significant difference for the specificity between IFOBT and SFOBT. After adjusting their detecting number of cancer to same level the cost of IFOBT was the lowest. among these FOBTs.3). The detected rate of early colorectal cancer was 60% for all of 3 protocols. 4). 41.6% approximately 48.3% of adenomas was found by the 3 protocols, and 87.5% of adenomas over 2 cm in diameter were detected by any one of FOBT. CONCLUSIONS: For the population with nice compliance 3 times of SFOBT will be recommended as a screening protocol of colorectal cancer. For that with poor compliance 2 times of IFOBT was recommended 3. The majority of adenomas in that advanced lesion existed might be detected by FOBT.

Adenoma↗

Safety and feasibility of an insulin adjustment protocol to maintain blood glucose concentrations within a narrow range in critically ill patients in an Australian level III adult intensive care unit.

OBJECTIVE: Recent data have shown a link between normal blood glucose levels and improved outcomes in intensive care patients. We wished to develop an insulin adjustment protocol for an adult intensive care unit to maintain blood glucose concentrations safely within a narrow range. METHODS: After a 6 month introductory period, an observational study was conducted during a 10 month period in an Australian level III intensive care unit to assess the safety and feasibility of an insulin adjustment protocol to maintain blood glucose concentrations safely within a narrow range. The protocol included a variable insulin infusion, a constant caloric source and frequent blood glucose level monitoring to detect and prevent hypoglycaemia. RESULTS: Over the 10 month period a total of 148 patients were studied using the protocol and represented 13 % of all intensive care unit admissions during this period. In total, there were 12,623 patient hours 'on protocol', with 5,603 blood glucose levels performed. The mean morning blood glucose level was 6.5 mmol/L and 49% of blood glucose levels were within the target range of 4.1 - 7.0 mmol/L. There were four recorded incidents of hypoglycaemia, defined as a blood glucose level of less than 2.2 mmol/L, the lowest at 1.5 mmol/L being the only symptomatic episode. The incidence of hyperglycaemia (blood glucose level > 10 mmol/L) was 13 % of all blood glucose level measurements. CONCLUSIONS: The insulin adjustment protocol with a constant caloric source and frequent blood glucose level monitoring was found to be safe and feasible in maintaining blood glucose concentrations within a narrow range in a mixed adult intensive care unit population.

Journal Article↗

Optimising cryopreservation protocols for haematopoietic progenitor cells: a methodological approach for umbilical cord blood.

Current cryopreservation protocols for haematopoietic cells have developed largely empirically and there is no consensus on an optimal method of preservation. These protocols, though providing sufficient cells to permit engraftment, can lead to cell loss of the order of 50 percent. In the context of umbilical cord blood such losses are unacceptable. Whilst an empirical approach can provide an acceptable level of recovery, the cryopreservation process can only be optimised by adopting a methodological approach. This paper provides an overview of just such an approach as illustrated by a study on CD34 cells from umbilical cord blood. It involves firstly the determination of membrane permeability parameters that can then be used to model safe addition and elution protocols for the chosen cryoprotectant, in this case dimethyl sulphoxide. This in turn permits cryoprotectant toxicity to be evaluated free from the confounding effect of osmotic damage caused by inappropriate addition and elution protocols. Finally, non-toxic concentrations of cryoprotectant may be investigated in a cooling rate study to provide an optimal cryopreservation protocol. Using the model, the effect on CD34 cells of current addition and elution protocols was also examined.

Antigens, CD34↗

Multi-factor designs. IV. How multi-factor designs improve the estimate of total error by accounting for protocol-specific biases.

Total error is often calculated as a combination of random error and fixed bias. However, the specific protocols used to estimate random error and fixed bias are themselves variable factors that can affect the estimate of total error. We refer to biases such as assay drift, sample-to-sample carryover, and reagent carryover as examples of fixed biases that are protocol-specific and distinguish them from other fixed biases. Failing to account for protocol-specific biases that are present will lead to incorrect estimates of total error when routine use of the assay involves a protocol different from that used to estimate total error. Multi-factor protocols are recommended to determine protocol-specific biases, which, if present, should be included in the estimate of total error.

Carcinoembryonic Antigen↗

[Evaluation of the degree of compliance in arterial hypertension protocol].

We report an evaluation of the hypertension protocol. Its aims were to assess the degree of compliance with its variables, the number, age and sex of hypertensives, and the rate in whom blood pressure levels were controlled in the patients included and not included in the protocol. The study was carried out in the Basauri Teaching Unit from January 1987 to June 1989. We reviewed all the parameters of the medical and nursing protocol of 1,429 patients diagnosed as hypertensive and the mean value of the three last blood pressure readings. A total of 67.2% of patients were included in the protocol. The compliance was higher than 80% in most of its items; 61.8% of the prescribed nursing controls were carried out. Blood pressure was less than or equal to 159/94 in 68.1% of patients. This rate was higher in the patients included in the protocol. The protocol was useful for the control of hypertension, although it should be modified directing it to primary care.

Adult↗

Anaerobic testing using the Wingate and Evans-Quinney protocols with and without toe stirrups.

Recently, considerable attention has been focused on the measurement of maximal anaerobic power. The Evans-Quinney (1981) protocol which considers leg volume as well as body weight in establishing optimal load settings on the bicycle ergometer has been shown to result in significantly higher anaerobic power outputs than the body weight-relative Wingate protocol. The purpose of this investigation was to compare the anaerobic power outputs during bicycle ergometer work using the Wingate and Evans-Quinney protocols with and without toe stirrups. Fifty male physical education and varsity athletes (average age 22.6 years) volunteered to participate in this experiment. All subjects performed a total of four maximal 30-second anaerobic power tests utilizing the force settings established by the Wingate (load [L] = 0.075 kp/kg body weight) and Evans-Quinney (L = -0.4914-0.2151 (weight, kg) + 2.1124 (leg volume, litre) protocol. The subjects were randomly assigned to one of four counterbalanced orders of test administration. Analysis of data indicated significant differences (P less than or equal to .05) among the variables of: 5-second peak power (W), 30-second anaerobic capacity (W) and percent fatigue as a function of test protocol. Significantly higher values were obtained for all variables under the Evans-Quinney test procedure using toe stirrups while the Wingate test procedure without toe stirrups showed significantly lower values for all variables. It was concluded that the Evans-Quinney load setting protocol with toe stirrups resulted in significantly higher power measures than any of the other treatments tested.

Adult↗

Using the proposed NCCLS protocol for evaluation of automated instruments.

We compared four instruments (Sequential Multiple Analyzer Computer (SMAC), AutoAnalyzer II (AAII), StatLyte, and PVA-4) for the determination of Na, K, Cl and CO2 using a protocol (PSEP-1) of the National Committee for Clinical Laboratory Standards (NCCLS) for evaluation of precision and accuracy of automated instruments. The protocol was applied concurrently to all four instruments. NCCLS has revised this protocol into three separate protocols by which the manufacturer can make performance claims. Protocols specifically aimed at the user are now under development. Although PSEP-1 is no longer in use, many parts of it have been incorporated into PSEP-2, PSEP-3 and PSEP-4. This article will describe the adaptation of the protocol to evaluate four automated instruments in our laboratory.

Autoanalysis↗

Pharmacokinetic comparison of direct antibody targeting with pretargeting protocols based on streptavidin-biotin binding.

UNLABELLED: Several groups are currently investigating antibody pretargeting as a strategy for improving radionuclide delivery. Pharmacokinetic modeling of these protocols permits analysis of pretargeting protocols under a broad range of possible experimental conditions. METHODS: We used previously developed pharmacokinetic models to predict the temporal uptake and spatial distribution of directly radiolabeled MAb, radiolabeled biotin given after pretargeting with streptavidinylated MAb and radiolabeled streptavidin given after pretargeting with biotinylated MAb in a microscopic, prevascular tumor nodule. Two dose regimens were investigated, as were the effects of internalization and degradation of antibody-antigen complexes (24-hr time constant). RESULTS: Simulations indicate that the protocol involving streptavidinylated MAb and radiolabeled biotin yields higher tumor-to-blood and tumor-to-lung ratios and relative exposures than the other protocols. In the absence of antigen internalization, the peak average molar concentration and MRT of biotin in the tumor nodule is comparable to that of directly radiolabeled MAb, and the spatial distribution of radionuclide is more uniform. When antigen internalization occurs, the peak average concentration and the MRT in the tumor nodule are lower than the corresponding values for directly radiolabeled MAb. CONCLUSION: In the absence of antigen internalization, the protocol involving streptavidinylated MAb and radiolabeled biotin offers pharmacokinetic advantages over the other two protocols.

Antibodies, Monoclonal↗

Management of the renal allograft recipient: immunosuppressive protocols for long-term success.

To determine the benefits of long-term cyclosporine (CsA) immunosuppression, renal allograft recipients were randomly assigned to a protocol of either: CsA+azathioprine (Aza)+prednisone (TD), or to a protocol in which CsA was discontinued from the regimen of Aza+prednisone (CsA D/C). With a mean follow-up of nearly 7 years since transplantation, 30/47 (64%) CsA D/C and 27/45 (60%) TD had functioning allografts. Although long-term survivals were similar, hazards of the CsA D/C protocol were evident (40% rate of acute rejection following CsA D/C). Conversely, continued CsA in the TD protocol provided the opportunity for prednisone reduction, or even complete prednisone withdrawal in selected patients. A TD protocol which can provide equivalent long-term success, and eventually lower or omit prednisone, is preferable to a protocol of CsA D/C.

Acute Disease↗

A distributed pharmacokinetic model of two-step imaging and treatment protocols: application to streptavidin-conjugated monoclonal antibodies and radiolabeled biotin.

Two-step imaging and treatment protocols involve injecting a suitably prepared monoclonal antibody that can bind both to a specific tumor antigen and to a second reagent which carries a drug or radionuclide. The second component is injected later, after the antibody has distributed throughout the target tumors and been largely cleared from the plasma and normal tissues. We introduce a mathematical model for the analysis of such protocols and apply it to the case of a streptavidinylated monoclonal antibody and radiolabeled biotin diffusing into small, prevascular, densely cellular nodules that represent either primary or metastatic tumors. We examine the distribution of streptavidinylated antibody and radiolabeled biotin within a tumor nodule and compare the two-step protocol to a one-step protocol using radiolabeled antibody. Our analysis predicts that (1) streptavidinylation reduces both the amount of antibody that distributes into the tumor nodule and the homogeneity of that distribution; (2) streptavidinylated antibody in the nodule can be saturated by initial plasma concentrations of free radiolabeled biotin substantially lower than the initial plasma concentration of free streptavidinylated antibody; (3) radiolabeled biotin diffuses rapidly, but binds so quickly that it will not penetrate deeply into the nodule if too low a dose is given. Hence, nonuniform localization of radiolabel may result from a "binding site barrier" to diffusion of either or both components; and (4) the two-step protocol permits imaging sooner after injection of radiolabeled material than the one-step protocol and produces a higher exposure in tumor relative to plasma, even in the presence of antigen turnover.

Bacterial Proteins↗

Protocol for writing cause-of-death statements for deaths due to natural causes.

The purpose of this article is to describe a protocol that provides suggestions and guides the clinical practice of writing cause-of-death statements for death certificates, autopsy reports, and other medical documents. The 16 members of the Autopsy Committee of the College of American Pathologists (CAP), Northfield, Ill, were appointed by the CAP officers based on demonstrated interest and expertise in autopsy performance and reporting. The committee perceived a need for specific instructions aimed at promoting consistency, accuracy, and completeness when writing cause-of-death statements. Development of a protocol was supported by CAP after approval by the CAP board of governors. The framework for the protocol was based on recommendations of the National Center for Health Statistics, Hyattsville, Md, with additional concepts added by the autopsy committee. The protocol for writing cause-of-death statements for natural causes is expected to foster consistency, accuracy, and completeness in the writing of cause-of-death statements. The quality of individual death certificates, autopsy reports, and national mortality data may improve through protocol usage. Application of the protocol principles should impose no ongoing costs at any user level because no new products or personnel are required for its application.

Autopsy↗

Modeling the management of protocols as the kernel of a healthcare information system.

A conceptual model of a health care structure is described. The management of protocols viewed as structured, flexible, and coherent descriptions of activities aimed to solve specific problems in the system is the kernel of the model. A global approach to the protocols is proposed. Each protocol is modeled from two points of view: the nature of the actions (clinical, administrative, programming, and control) and the life cycle of protocols (theoretical, customized, and performed). In our approach the patient folder not only records information about the patient, but also contains and controls the execution of the protocols planned to be performed on the patient. As a consequence, all aspects related to protocol execution are chronologically reported in the folder in a federated manner. In this way the patient folder becomes a complex, multifaceted object, able to capture all information needed to evaluate the patient evolution in a given period.

Hospital Information Systems↗

Prospective randomized study of an ultrashort gonadotrophin-releasing hormone agonist versus a modified suppression protocol for ovarian stimulation in intracytoplasmic sperm injection cycles.

To compare oocyte quality and clinical outcome after an ultrashort or a modified suppression gonadotrophin-releasing hormone agonist (GnRHa) protocol for ovarian stimulation in intracytoplasmic sperm injection (ICSI) cycles, we conducted a prospective randomized study of 60 consecutive couples with severe male infertility admitted for their first in-vitro fertilization (IVF) and ICSI attempt. More cycles were cancelled after the ultrashort protocol (8/30) than after the modified suppression protocol (3/30), although the difference was not significant. There were no cases of severe ovarian hyperstimulation syndrome (OHSS) in the ultrashort group compared to three cases in the suppression group. The percentage of mature metaphase II oocytes recovered in both groups was similar (88 versus 86%), as were the fertilization or cleavage rates after ICSI. In the ultrashort group, a total of 64 embryos was replaced in 22 transfers (mean 2.9 embryos per transfer), resulting in three first trimester abortions and seven deliveries. In the suppression group, 11 deliveries were achieved after transfer of a total of 75 embryos in 27 patients (mean 2.8 embryos per transfer). In conclusion, there was no apparent difference between the two GnRHa protocols in terms of oocyte quality and clinical outcome. However, because of the lower rate of severe OHSS, in our study the ultrashort protocol was more appropriate for ovarian stimulation in ICSI cycles than the modified suppression protocol.

Adolescent↗