Illness due to molluscan shellfish--England and Wales.
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Six month old, laboratory-raised goldfish (Carassius auratus) used in morphologic studies of retinal development developed progressive fraying and deterioration of the tail and fins that reached epizootic proportions and culminated in fungal infection and death. Diagnostic histopathology showed marked skeletal muscle degeneration in the muscles of the tail and caudal body wall compatible with a vitamin E/selenium deficiency. Ration analysis demonstrated inadequate vitamin E in the commercial shrimp-based diets fed to the goldfish fry. Replacement of shrimp-derived diets by a commercial trout diet containing adequate levels of vitamin E was curative.
Twenty-five laboratories were sent 2 materials, one an acetone powder of lobster digestive gland, the other, the oil which had been extracted during preparation of the powder. Each laboratory was requested to measure the levels of a suite of polycyclic aromatic hydrocarbons in both materials. The response was poor with only 10 laboratories submitting results. Both intra- and interlaboratory precisions were poor; the interlaboratory error was so great as to preclude statistical analysis of the error. Relative standard deviations for oil results determined by liquid chromatography ranged from 39 to 96%.
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An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of saxitoxin (STX). Antibodies against STX were demonstrated in rabbits 5 weeks after immunizing with STX-bovine serum albumin (STX-HCHO-BSA). In the ELISA, STX-HCHO-BSA or polylysine-STX was coated onto the microtiter plate, followed by incubation with standard toxin and anti-STX antibody. The amount of antibody bound to the solid phase was determined by incubation with goat anti-rabbit IgG peroxidase conjugate and a reaction with chromogenic substrate. Competitive indirect ELISA revealed that the anti-serum did not cross-react with either carbamoyl-neo-STX-sulfate or tetrodotoxin. The antibodies for STX cross-reacted with decarbamoyl-STX and neo-STX about 56% and 16% as much as they did with STX, respectively. The lower detection limits for STX, decarbamoyl-STX, and neo-STX in this system were about 25, 45, and 156 pg per assay, respectively. When STX added to clams or mussels was assayed, the detection limit for STX was about 50-100 ppb, and recoveries were in the range of 86.8-107%.
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