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PubMed1998

Biodegradation kinetics of naphthalene in nonaqueous phase liquid-water mixed batch systems: comparison of model predictions and experimental results.

A model is formulated to describe dissolution of naphthalene from an insoluble nonaqueous phase liquid (NAPL) and its subsequent biodegradation in the aqueous phase in completely mixed batch reactors. The physicochemical processes of equilibrium partitioning and mass transfer of naphthalene between the NAPL and aqueous phases were incorporated into the model. Biodegradation kinetics were described by Monod's microbial growth kinetic model, modified to account for the inhibitory effects of 1,2-naphthoquinone formed during naphthalene degradation under certain conditions. System parameters and biokinetic coefficients pertinent to the NAPL-water systems were determined either by direct measurement or from nonlinear regression of the naphthalene mineralization profiles obtained from batch reactor tests with two-component NAPLs comprised of naphthalene and heptamethylnonane. The NAPLs contained substantial mass of naphthalene, and naphthalene biodegradation kinetics were evaluated over the time required for near complete depletion of naphthalene from the NAPL. Model predictions of naphthalene mineralization time profiles compared favorably to the general trends observed in the data obtained from laboratory experiments with the two-component NAPL, as well as with two coal tars obtained from the subsurface at contaminated sites and composed of many different PAHs (polycyclic aromatic hydrocarbon compounds). The effects of varying the NAPL mass and the naphthalene mole fractions in the NAPL are discussed. It was observed that the time to achieve a given percent removal of naphthalene does not change significantly with the initial mass of naphthalene in a fixed volume of the NAPL. Significant changes in the mineralization profiles are observed when the volume (and mass) of NAPL in the system is changed.

Biodegradation, Environmental↗
PubMed1999

Biodegradable polymer film as a source for formation of human fetal retinal pigment epithelium spheroids.

PURPOSE: To evaluate the attachment of human fetal rctinal pigment epithelial (HFRPE) cells to a biodegradable polymer film with subsequent formation of spheroids in vitro. METHODS: Ten biodegradable polymer films with different compositions were examined for their physical properties and ease of manipulation under a dissecting microscope. The film with the most suitable handling characteristics was chosen, and a purely isolated sheet of HFRPE cells was attached to it. The purity of the cells was assessed by their pigmentation and expression of cytokeratin. Proliferation was assessed by incorporation of 5-bromo-2'-deoxyuridine (BrdtJ). Cellular structure was analyzed under light and electron microscopes, and the functional capability of the cells was evaluated by rod outer segment (ROS) phagocytosis. RESULTS: The polymer film with composition 50:50 poly (DL-lactide) (PLA)/poly (DL-lactide-co-glycolide) (PLG) with an inherent viscosity of 1.03 dl/g was found to be the most suitable for handling under the microscope. Sheets of HFRPE cells attached to the polymer films within 48 hours and began to form spheroids. All the isolated cells were pigmented and expressed cytokeratin. They possessed a cuboidal morphology, numerous apical microvilli, and no sign of dedifferentiation. HFRPE cells produced extracellular matrix (collagen filaments) on their basal side, filling the cavities of the polymer film. The cells subsequently proliferated, incorporated BrdU, migrated onto the culture plate to form monolayers, and phagocytized ROS. CONCLUSIONS: Biodegradable polymer films can be used as a scaffold for the adhesion of the HFRPE sheet and formation of spheroids. Spheroids represent a source of high density and well-differentiated HFRPE cells that are easy to transfer. Furthermore, the stricture of the membrane makes it suitable for additional applications.

Biodegradation, Environmental↗
PubMed1999

Biodegradable screw fixation of the syndesmosis together with metallic osteosynthesis. Preliminary experience of 7 ankles.

BACKGROUND AND AIMS: In ankle fractures with separation of the tibiofibular mortise a metallic syndesmosis screw is generally used. As a rule, this transfixing screw is removed by a separate operation 6 to 8 weeks later. Usually the fracture fixation implants are removed by a second operation later on. In order to eliminate separate removal of the transfixing screw, we used a biodegradable syndesmosis screw in a pilot clinical study. PATIENTS AND METHODS: We treated seven consecutive patients with malleolar fractures and separation of the syndesmosis by an ordinary metallic plating and screw fixation of the fractures and biodegradable polyglycolic acid (PGA) screw transfixation of the syndesmosis. RESULTS AND CONCLUSIONS: All the patients ended up with an acceptable result and stable ankle mortise. One of the ankles, which was the only one fixed by two transfixing PGA screws instead of one screw, had transient sinus formation and intraosseal osteolysis. The final result was good also in this case. The ankle mortise can be fixed safely by biodegradable screws in connection with metallic osteosynthesis of malleolar fractures. Thus a separate removal of the transfixing material is possible to eliminate.

Adult↗
PubMed1999

Biodegradation of phenol by bacterial strains from petroleum-refining wastewater purification plant.

The ability of four strains of bacteria derived from a biological petroleum-refining wastewater purification plant to carry out the biodegradation of phenol was studied. Two of the strains belonging to the genus Pseudomonas were found to be characterised by high effectiveness of the removal of phenol which was used as sole carbon and energy source (the strains were designated P1 and P2). In turn the effect of inoculum size, initial concentration of substrate (500 and 1,000 mg phenol/L) and temperature (10, 20 and 30 degrees C) on the rate of phenol degradation by strains P1, P2 and mixture of both was investigated. It was found that strain P1 which was identified as Pseudomonas fluorescens degraded phenol better than strain P2--Pseudomonas cepacia. The rate of phenol biodegradation was significantly affected by size of inoculum and temperature of incubation. Phenol was removed the fastest with the highest inoculum used. The optimal temperature was about 20 degrees C. At 10 and 30 degrees C the process of biodegradation was visibly inhibited. The rate of phenol utilisation was also found to decrease with increased concentration of substrate.

Biodegradation, Environmental↗
PubMed2000

Laparoscopic augmentation cystoplasty with different biodegradable grafts in an animal model.

PURPOSE: Recently a variety of biodegradable organic materials have been used for bladder wall replacement. We sought to study the effectiveness of 4 different types of biodegradable materials for bladder augmentation using laparoscopic techniques. MATERIALS AND METHODS: Thirty one minipigs underwent successful transperitoneal laparoscopic partial cystectomy and subsequent closure (6 control) or patch augmentation (25): porcine bowel acellular tissue matrix (ATM) (6), bovine pericardium (BPC) (6), human placental membranes (HPM) (6) or porcine small intestinal submucosa (SIS) (7). An intracorporeal suturing technique with the EndoStitch device (U.S. Surgical, Norwalk, CT) and Lapra-Ty clips (Ethicon, Enodsurgery Inc. Cincinnati, OH) was used to anastomose the graft to the bladder wall. Postoperatively, a urethral catheter was left for one week. Bladders were evaluated by cystoscopy at 6 and 12 weeks and harvested at 12 weeks. RESULTS: Grafts remained in place in all groups except for the BPC group, where all grafts failed to incorporate. For the ATM and SIS groups, at 6 weeks, there was mucosal coverage of the grafts without evidence of encrustation. In the control group, at 12 weeks, the bladder capacity was 23% less than preoperatively. In the ATM, HPM and SIS groups, at 12 weeks, the bladder capacities were larger than preoperatively by 16%, 51% and 43% respectively; also the grafts had contracted to 70%, 65%, and 60% of their original sizes, respectively. Histologically, there was patchy epithelialization of ATM and SIS grafts with a mixture of squamoid and transitional cell epithelia. The graft persisted as a well-vascularized fibrous band in HPM, ATM, and SIS without evidence of significant inflammatory response. CONCLUSION: A laparoscopic technique for partial bladder wall replacement using a free graft is feasible. The biodegradable grafts of ATM, HPM and SIS are tolerated by host bladder and are associated with predominantly only mucosal regeneration at 12 weeks post-operatively.

Animals↗
PubMed2000

Nanospheres of biodegradable polymers: a system for clinical administration of an anticancer drug paclitaxel (Taxol).

INTRODUCTION: Paclitaxel is a natural antineoplastic drug. It has been approved by the Food and Drug Administration (FDA) for the treatment of various cancers, especially ovarian and breast cancers with the best effect over other anticancer agents. Due to its high hydrophobicity however, an excipient such as Cremophor EL has to be used, which causes additional serious side effects. This study was intended to investigate the feasibility to apply nanospheres of biodegradable polymers as an alternative system to achieve best results with least side effects. MATERIALS AND METHODS: FDA-approved biodegradable polymers such as poly(D, L-lactic-co-glycolic acid) (PLGA) and poly(D, L-lactic acid) (PLA) were used to fabricate paclitaxel-loaded nanospheres by the solvent extraction/evaporation technique. The nanospheres fabricated in various conditions were characterised by laser light scattering for size and size distribution, scanning electron microscopy (SEM) and atomic force microscopy (AFM) for surface morphology, X-ray photoelectron spectroscopy (XPS) for the chemical structure of the surface. The drug encapsulation efficiency (EE) and the in vitro release kinetics were measured by HPLC. RESULTS: The products ranged from 300 nm to 700 nm with low polydispersity. The in vitro drug release can last more than 6 months at an approximately constant rate after an initial burst. The release kinetics can be fully controlled by the material used and the fabrication conditions. CONCLUSIONS: Nanospheres of biodegradable polymers can be an ideal carrier for pacitaxel to eliminate the side effects caused by the excipient currently used in clinical administration. The technique developed can also be applied to other anticancer drugs.

Antineoplastic Agents, Phytogenic↗
PubMed2001

Biodegradation of poly(tetramethylene succinate-co-tetramethylene adipate) and poly(tetramethylene succinate) through water-soluble products.

Poly(tetramethylene succinate-co-tetramethylene adipate) (PBSA) and poly(tetramethylenesuccinate) (PBS) were hydrolyzed experimentally into water-soluble oligomers and monomers by Chromobacterium extracellular lipase. The oligomers were identified by high-performance liquid chromatography-mass spectrometry and 1H-nuclear magnetic resonance, which indicated that a total of 28 oligomer species were liberated from PBSA, and that 13 of them were identical to the hydrolysates from PBS. Moreover, 20 of the species were polyester-based compounds of monomer units, and the other 8 species were small amounts of diurethane compounds. Bis(hydroxybutyl) succinate (BSB) and bis(hydroxybutyl) hexamethylene dicarbamate (BHB) were the typical oligomers and were chemically synthesized. Biodegradability of BSB and BHB was examined for 28 d in the activated sludge, and analysis of the results of this study indicated that the final conversion rate of constituent carbon to carbon dioxide was estimated at 80 mol% for BSB and 10 mol% for BHB. The remaining amount of carbon in the undegraded BHB was 20 mol%. In the presence of BSB, the biodegradability of BHB was increased by about 1.5 times. The suggestion was made that BSB induced a growth of microorganisms and helped BHB degradation. This is consistent with the observation that the biodegradation of BHB in native soil for 60 d reached > 60%.

Adipates↗
PubMed2000

Investigations on the changes in anaerobic biodegradability and biotoxicity of an industrial microbial biomass induced by a thermochemical pretreatment.

In the anaerobic digestion of solid wastes, hydrolysis is the rate limiting step and physicochemical pretreatment is often required to promote solubilization of organic matter. As an illustration, anaerobic digestion was limited by the substrate solubilization step during continuous cultures performed with an industrial microbial biomass. In optimal operating conditions determined for the hydrolysis-acidogenesis step (T = 35 degrees C; pH = 8.5; OLR = 5.4 g COD/l.d) 50.6% COD solubilization was achieved. A thermochemical pretreatment based on sodium hydroxide addition, was used in order to enhance COD solubilization. Optimal conditions for COD solubilization were pH = 12, T = 140 degrees C for 30 minutes. In these conditions, 70% COD solubilization was achieved. However, anaerobic biodegradability of the pretreated substrate was not improved and remained near 40%. The poor anaerobic biodegradability performances were attributed to the soluble molecules generated during the thermochemical pretreatment that were refractory and/or inhibitory to anaerobic microorganisms. Fractionation of the soluble pretreated microbial biomass by two methods (treatment with adsorbent resins and precipitation by pH adjustment) demonstrated that high molecular weight compounds (> 100 kDa) are involved in the poor biodegradability and in the biotoxicity observed. Partial decolorization through resin use and acid precipitation remove these compounds. The consequence of their removal was an increase of the production of biogas.

Acetates↗
PubMed2001

[Biodegradation behavior of ethylenediaminetetraethylene acid].

Aerobic and anaerobic degradability of ethylenediaminetetraethylene acid(EDTA) were measured on Warburg respirometer and anaerobic batch reactors. Biodegradation of EDTA as a single substance was poor, but biodegradation of EDTA with co-substrate could be improved. The wastewater containing EDTA can be treated by activated sludge process. The EDTA removal efficiency was 80% under the circumstance HRT 16 h and EDTA removal efficiency reached to 92.5%-95.1% when HRT was 20 h. The main factor affecting the biodegradability of EDTA is sludge remained time.

Biodegradation, Environmental↗
PubMed2001

Aerobic and anaerobic biodegradability of a flocculant polymer.

Flocculant polymers are used to improve the efficiency of separation processes used in wastewater treatment. The subsequent fate and effects of these additives are uncertain, however, with some previous reports indicating them to be biodegradable while others indicate complete recalcitrance. The biodegradability of a common flocculant polymer was therefore evaluated, using both aerobic and anaerobic batch assays. Knowledge of the polymer's chemical composition also allowed degradation stoichiometries to be calculated for complete biodegradation and also for incomplete degradation to several hypothesized end products. Results showed conclusively that the polymer was subject to partial degradation by both aerobic and anaerobic cultures. Measured oxygen consumption under aerobic conditions, and gas production under anaerobic conditions, both indicate that the partial destruction of pendant cationic moieties occurs, but that the polymer's CH2 backbone remains essentially intact. These results allow seemingly contradictory previous reports to be explained. The findings are relevant to the environmental fate of these polymers as well as certain treatment process effects.

Bacteria, Aerobic↗
PubMed2001

[Effect of temperature on biodegradation of the petrochemicals in contaminated soil].

Temperature is a significant factor of the natural attenuation of the polluted soil. The effect of the temperature on the biodegradation rates and the half-life of the petrochemicals in contaminated soil were evaluated in this paper by the simulative experiments in laboratory. The results showed that the relationship between the temperature and the biodegradation rates was consistent with the exponential equation: K = 3145exp(-5233/T). According to the prediction of half-life of the biodegradation rates by the equation, the half-life at 5 degrees C, 10 degrees C, 20 degrees C and 30 degrees C were 1499 days, 1075 days, 572 days and 317 days respectively.

Biodegradation, Environmental↗
PubMed2002

Effect of sorption and desorption resistance on aerobic trichloroethylene biodegradation in soils.

Biodegradation of trichloroethylene (TCE) by toluene-degrading bacteria was measured under aerobic conditions in aqueous and soil-slurry batch microcosms. For soil-phase experiments, a freshly contaminated soil and a soil containing only the desorption-resistant fraction of TCE were tested. In both cases, presence of soil resulted in biodegradation rates substantially lower than those determined in the absence of soil. In aqueous-phase experiments, an appreciable increase in the rate and extent of TCE biodegradation was observed in microcosms when toluene was added multiple times. The TCE degradation rates were clearly correlated with toluene dioxygenase (TOD) enzyme activity over time, thus providing an indication of the cometabolic pathway employed by the microbial population. In soil-slurry experiments containing freshly contaminated soil, a TCE degradation rate of approximately 150 microg TCE/kg/h was observed during the first 39-h period, and then the TCE degradation rate slowed considerably to 0.59 and 0.84 microg TCE/kg/h for microcosms receiving one and two additions of toluene, respectively. The TCE degradation rates in soil-slurry microcosms containing the desorption-resistant fraction of TCE-contaminated soil were approximately 0.27 and 0.32 microg TCE/kg/h in microcosms receiving one and two additions of toluene, respectively. It is clear from these results that mass transfer into the aqueous phase limited bioavailability of TCE in the contaminated soil.

Adsorption↗
PubMed2002

Toxicity and biodegradability of high strength/toxic organic liquid industrial effluents and hazardous landfill leachates.

Industrial effluents and leachates from hazardous landfill sites were tested for toxicity using the anaerobic toxicity assay. This test was done on several industrial effluents (brewery spent grain effluent, a chemical industry effluent, size effluent), and several hazardous landfill leachates giving vastly different toxicity results. The brewery effluent, spent grain effluent and size effluent were found to be less toxic than the chemical effluent and hazardous landfill leachate samples. The chemical industry effluent was found to be most toxic. Leachate samples from the H:h classified hazardous landfill site were found to be less toxic at high concentrations (40% (v/v)) while the H:H hazardous landfill leachate samples were found to be more toxic even at low concentrations of 4% (v/v). The 30 d biochemical methane potential tests revealed that the brewery effluent, organic spent grain effluent and size effluent were 89%, 63%, and 68% biodegradable, respectively. The leachate from Holfontein hazardous landfill site was least biodegradable (19%) while the chemical effluent and Aloes leachate were 29% and 32% biodegradable under anaerobic conditions.

Bacteria, Anaerobic↗
PubMed2002

Microbial binding and biodegradation of mycotoxins.

This contribution provides an overview of literature data on the microbial binding and biodegradation of mycotoxins. These data and preliminary results from our own laboratory suggest that mycotoxin-bacterial binding or biodegradation is a realistic process and encourages the screening of bacterial strains and their biodegradation potential.

Animals↗
PubMed2002

[Biodegraded characteristics of MGP-wastewater by domestic and screened organisms].

Among 16 bacteria that were screened out from 5 activated sludge, S-2, Y-3, XH-3 and their mixed strains M-3 were selected to biodegrade MGP (Manufacture gas from petroleum)-wastewater. They can remove 38.4%-44.0% CODCr, 95.4%-97.0% hydroxybenzene and 47.1%-53.7% aromatic compound after 60 h treatment, but the removal rate of NH4(+)-N was only 14.0%-17.6%. About 77 toxic organic compounds were detected by GC/MS analysis, the aromatic compounds which had 3 rings and 4-6 rings can be biodegraded by the bacteria. The removal rate of CODCr, NH4(+)-N and extractable organic compound were improved 13.1%-22.9%, 18.4%-22.7% and 13.1%-18.1% respectively when glucose was added in the wastewater, and when ethanol was existing in the wastewater, the corresponding increase were 12.5%-21.2%, 29.7%-42.2% and 6.7%-7.7%. The total removal rates of aromatic compound were increased 17.7%-21.7%, 15.4%-21.2%. The screened strains can improve the biodegradation rate of MGP-wastewater.

Bacteria↗
PubMed2000

[Study on biodegradation of phenols in river water].

This study on the biodegradation of phenols (phenol, o-methylphenol, o-chlorophenol and resorcinol) in river water was conducted by changing the main influential factors respectively. The results of experiment showed the phenols were greatly degradated in mild temperature, neutral pH and plenty of oxygen and the rank of the phenols biodegradation from great to small was resorcinol, phenol, o-methylphenol and o-chlorophenol. Presented in this paper is also a discussion on the biodegradation mechanism of phenols.

Biodegradation, Environmental↗
PubMed2002

Molecular and clinical consequences of in vivo silicones biodegradation.

The object of the publication is the presentation of data referring to in vivo silicones biodegradation and their potential clinical and molecular consequences. Summing up, one needs to state that the products of silicones biodegradation are not biologically indifferent. In particular, different types of silanols are inhibitors of proteolytic enzymes and regulators of many metabolic processes as well as affect the cell growth and division, which distinctly indicates the potentially strong pharmacological action. The main objective of the molecular consequences of the biodegradation of siloxanes was the verification what impact siloxanes of various molecular weights and of various chain lengths exert on the conformational stability of biological molecules.

Absorbable Implants↗