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At least 829 records · Page 46Linked to original sources

Dental disease in hill sheep: an abattoir survey.

Heads from 478 aged sheep of a predominantly hill breed population were examined in detail for evidence of dental disease, using both soft and hard tissue measurements around incisor and cheek teeth. Almost 60 per cent of this population (all over 2 1/2 years of age and with a mean age between 7 and 8 years) had either loose or missing teeth. Malalignment of cheek teeth and/or incisors was also common. Pocketing was seen in 87 per cent of the population, and was significantly correlated with tooth looseness. There was also a significant relationship between incisor and specific cheek tooth pocket depths. Whilst local alveolar bone loss was found, it was not a reflection of more general skeletal deterioration. The survey confirms the high incidence of broken mouth in the national flock and shows that wherever incisor problems occur there is a likelihood that cheek tooth disease is also present, a relationship not readily appreciated clinically.

Animals↗

Stress-induced cervical lesions.

The increasing occurrence of dental lesions at the cervical surfaces requires more knowledge of the causes of the process. Acidic and abrasive mechanisms have clearly been documented as causes but the stress theory by Lee and Eakle is still controversial. This report describes several incidences of possible stress-induced lesions according to the characteristics described by Lee and Eakle. The occurrences of subgingival lesions lend credence to the stress-induction theory by exclusion of other superimposing etiologic factors. With the current concepts, a perceptive approach to the treatment of cervical lesions can be executed.

Bite Force↗

The wear of enamel opposing shaded ceramic restorative materials: an in vitro study.

The wear rate of intact human enamel opposed by Olympia porcelain gold, Dicor, Ceramco porcelain, and externally shaded Dicor and Ceramco was investigated with an artificial oral environment. The enamel-material couples were subjected to 300,000 masticatory cycles at a maximal occlusal force of 13.4 N while they were continuously bathed with 37 degrees C deionized water. Both the enamel and material surfaces were analyzed by use of a three-dimensional surface monitoring computer program, AnSur, to record the removal of the material and the maximal loss of vertical height. The enamel opposing the externally shaded materials abraded two to five times more than that opposing the unshaded materials and 10 to 15 times more than enamel opposing gold. The wear rates for enamel opposing the gold and unshaded Dicor were similar both in the removal of material and in the loss in vertical height.

Bite Force↗

The anorexia nervosa syndrome.

This article reviews the medical and dental literature on the anorexia nervosa syndrome and presents the oral and systemic manifestations of this serious disease process. A case is reported which demonstrates the generalized destruction of tooth structure, primarily as a result of the direct effect of gastric acid on this patient's dentition during a 5-year period of self-induced vomiting. The systemic, psychological, and social aspects of this syndrome are classically illustrated in this case history.

Adult↗

Perimolysis: unveiling the surreptitious vomiter.

Perimolysis is a dental condition linked to chronic regurgitation. When perimolysis is found in the patient who denies vomiting, one must suspect anorexia nervosa, a disorder with a high rate of morbidity and mortality. The dental literature has not provided guidelines for confirming the suspicion of surreptitious vomiting. The purpose of this case report is to describe our approach, using simple blood and urine studies, which establishes whether a patient who has perimolysis but denies vomiting is a surreptitious vomiter.

Adult↗

An unusual case of black teeth.

Loss of enamel and a deep black stain of the teeth in a 40-year-old diabetic patient are strongly suggested to be caused by the daily consumption of a cheap white wine and, possibly, by the chewing of cayenne. The wine proved to be rather acid, thereby promoting abrasion as a result of gnashing, and to contain a high concentration of tannin. The exact role of the tannins is described.

Adult↗

Assessment of tooth wear in an ageing population.

A random sample of 2222 adults aged 45 years and over was taken from the age/sex registers of two general medical practices in Newcastle upon Tyne, and asked to take part in a dental health survey. A total of 1002 individuals agreed to take part and were visited in their homes. Five hundred and eighty-six subjects were dentate and able to undergo an assessment of tooth wear using the Tooth Wear Index (TWI). Increasing wear with increasing age was observed for all cervical and occlusal/incisal tooth surfaces. Occlusal/incisal surfaces displayed some of the highest mean wear scores, especially in the older age cohorts. With the exception of lingual surfaces of maxillary anterior teeth, no significant variation in tooth wear with age was noted for buccal or lingual surfaces. Greater mean wear scores were observed among males than females, but there was little variation between subjects of different social class backgrounds. The threshold levels of wear suggested for use with the TWI may require modification for use among elderly populations.

Age Factors↗

Carbohydrate uptake in the oral pathogen Streptococcus mutans: mechanisms and regulation by protein phosphorylation.

Streptococcus mutans is the primary etiological agent of dental caries in man and other animals. This organism and other related oral streptococci use carbohydrates almost exclusively as carbon and energy sources, fermenting them primarily to lactic acid which initiates erosion of tooth surfaces. Investigations over the past decade have shown that the major uptake mechanism for most carbohydrates in S. mutans is the phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS), although non-PTS systems have also been identified for glucose and sucrose. Regulation of sugar uptake occurs by induction/repression and inducer exclusion mechanisms in S. mutans, but apparently not by inducer expulsion as is found in some other streptococci. In addition, ATP-dependent protein kinases have also been identified in S. mutans and other oral streptococci, and a regulatory function for at least one of these has been postulated. Among a number of proteins that are phosphorylated by these enzymes, the predominant soluble protein substrate is the general phospho-carrier protein of the PTS, HPr, as had previously been observed in a variety of Gram-positive bacteria. Recent results have provided evidence for a role for ATP-dependent phosphorylation of HPr in the coordination of sugar uptake and its catabolism in S. mutans. In this review, these results are summarized, and directions for future research in this area are discussed.

Carbohydrates↗

Influence of a soft drink with low pH on enamel surfaces: an in vitro study.

INTRODUCTION: The purpose of this study was to investigate, in vitro, the short-term effect of a soft drink (Coca Cola, Coca Cola Company, Atlanta, Ga) with low pH on enamel surfaces at different levels of intake. Effects on labial and palatal surfaces were also compared. METHODS: Thirty human enamel blocks (labial and palatal enamel surfaces) were randomly divided into 5 groups-1 control and 4 experimental. For 7 days, the enamel surfaces in the experimental groups were immersed in Coca-Cola at different frequencies: 1, 2, or 3 times per day for 20 minutes each, with each immersion followed by 1 hour in artificial saliva. Specimens in the fourth group were immersed for 1 minute in the soft drink followed by 3 minutes in artificial saliva, and the cycle repeated for 20 minutes to better simulate drinking habits. Control specimens remained in physiological serum at room temperature. The measurements were made at baseline (T0), day 3 (T1), and day 7 (T2) with a Knoop hardness tester. Analysis of the variance was used to find differences between the groups. For the multiple comparisons, the Dunnett test was used. The statistical level was accepted at P < .05. RESULTS: The experimental groups showed significant reductions in microhardness values. Differences between the test groups were not statistically significant, nor were differences between the labial and the palatal surfaces. CONCLUSIONS: Soft drink intake, even of relatively short duration, can reduce enamel microhardness. The frequency of intake seems not to play a decisive role on enamel microhardness. Care should be taken to avoid soft drinks during orthodontic treatment with fixed appliances.

Analysis of Variance↗

Proteomic characterization of the acquired enamel pellicle under acidic challenges at early and mature formation stages.

OBJECTIVES: This study aimed to characterize acquired enamel pellicle (AEP) proteomic changes after exposure to citric acid (CA) and hydrochloric acid (HCl) under different pellicle formation times (3 and 120&#x202f;min) in the same volunteers. DESIGN: Nine healthy volunteers participated in this randomized crossover in vivo study. The AEP was allowed to form for 3 or 120&#x202f;min and subsequently exposed for 10&#x202f;s to deionized water (control), 1% CA (pH 2.5), or 0.01&#x202f;M HCl (pH 2.0). Pellicle samples were collected, followed by protein extraction, tryptic digestion, and analysis by nanoliquid chromatography (nanoLC) coupled to mass spectrometry (MS) with MSE (data-independent acquisition; nanoLC-MS&#x1d31;). Label-free quantitative proteomics were performed for relative quantification using t-test (p&#x202f;<&#x202f;0.05). RESULTS: At 120&#x202f;min, CA exposure markedly reduced several typical AEP proteins, especially acidic proline-rich proteins (PRPs). Conversely, basic PRPs were upregulated, suggesting acid-resistance protein signature. At 3&#x202f;min, basal-layer proteins (PRPs, cystatins, histatins and mucins) were more abundant. Hemoglobins increased 6-8-fold (up to 150-fold in 3&#x202f;min control), suggesting association with early pellicle formation and an acid-resistant protein signature. CA exposures for 120&#x202f;min also upregulated typical AEP proteins (PRPs, mucins, cystatins, immunoglobulins), while HCl exposure depleted albumins and lactotransferrin. CONCLUSION: Intrinsic and extrinsic acids induce distinct proteomic signatures in the AEP. Hemoglobin and PRPs appear consistently enriched in the early pellicle layer, reflecting an initial acid-resistant protein signature. These findings provide new insights into the molecular remodeling of the AEP following intrinsic and extrinsic acid exposure, highlighting proteins potentially involved in early-stage pellicle formation.

Humans↗

Property variations in the prism and the organic sheath within enamel by nanoindentation.

Atomic force microscopy (AFM) combined with nanoindentation technique was used to definitely, site-specifically, test the nanomechanical properties, including nanohardness and elastic modulus, of the isolated domains within single enamel, the prisms and the surrounding sheaths, of mature human maxillary third molars. In this way, it is for the first time that evident differences of nanomechanical properties were revealed between these domains. The nanohardness and elastic modulus of the sheaths were about 73.6% and 52.7% lower than those of the prisms, respectively. Measuring the residual impressions with AFM supported the similar conclusion. The variations of mechanical properties in these domains are considered to be mainly relative to their different component and fibrils arrangement.

Biomechanical Phenomena↗

The reproducibility of ultrasonic enamel thickness measurements: an in vitro study.

OBJECTIVES: The diagnosis of tooth wear is mostly based on visual scoring systems. Recently, methods measuring ultrasonic enamel thickness have been proposed. The aim of this study was to evaluate the reproducibility and evaluate factors affecting variation of ultrasonic enamel thickness measurements in an in vitro set up. METHODS: Four observers (1-4) in two institutes (G and S) performed ultrasonic enamel thickness measurements at buccal and palatal surfaces of extracted teeth placed in a phantom jaw. A Panametrics 25DL thickness gauge (Panametrics, Waltham MA, USA), with a 15 MHz transducer, covered by a plexi-glass delay line with a tip diameter of 2 mm was used, coupled to a laptop computer. After one week these measurements were repeated. Subsequently four selected teeth were measured 20 times at a fixed buccal site by one observer. Finally, two observers made ink marks with the instrument probe tip on the buccal and palatal surfaces of all teeth. This was repeated after one week. Digital images were made of the marked teeth and analysed to determine variation in positioning. The underlying enamel thickness variation was subsequently estimated. Results were analysed by calculating the 95% range of measurement variation (RMV, 4 x standard deviation) for each experiment. RESULTS: The main reproducibility experiment showed a 95% RMV of 0.79 mm (observer 1G), 0.64 mm (observer 2G), 0.63 mm (observer 3S), 0.71 mm (observer 4S), 0.51 mm (observer 1S). The repeatability experiment showed a mean 95% RMV of 0.26 mm. The probe position variation in the cervical-incisal direction resulted in an estimated thickness 95% RMV of 0.10 mm (1G) and 0.09 mm (2G). CONCLUSIONS: It was concluded that, due to measurement variation, thickness changes of less than 0.33 mm cannot be detected reliably. Probe positioning and a poor repeatability were responsible for only a small part of the total variation. Hence other factors influencing variation in enamel thickness are still to be identified.

Dental Enamel↗