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PubMed1999

[Studies on the screening high yield acid protease producing strain L336 by combining microwave irradiation with chemical inducing].

A high yield acid protease producing strain Aspergillus usamii L336 was screened from its parent strain B1 by combining microwave irradiation with inducing of nitrosoguanidine and Li2SO4. By incubation of L336 in shaking flask at 31 degrees C for 88 h, its enzyme activity was 5500 u/mL, and its properties for high yield producing acid protease remained stable after many times of subculture and storage for two months.

Aspartic Acid Endopeptidases↗
PubMed2003

Orthodontic radiographic procedures--how safe are they?

Parents of children subjected to radiographic procedures for orthodontic purposes occasionally express concern about possible radiation overdose to sensitive structures in the head and neck region. The following study was designed to determine this. Twenty Harshaw lithium fluoride thermoluminescent dosimeters (TLDs) were irradiated using a calibrated RT 100 X-ray source. These were read in a Toledo 654 TLD reader. The readings of all dosimeters fell within 10% on either side of the mean reading for the dosimeters. Twelve were finally selected which fell closest to the mean. Six of the TLDs were randomly selected and placed in a Rando female phantom in a position corresponding to the lens of the eye-3 in the left and 3 in the right eye. A standard pantomogram was taken of the phantom using an Orthophos machine. The TLDs were then replaced by another two groups of 3 in the same positions in the phantom and a lateral cephalogram taken on the same machine. The TLDs were read in the Toledo reader. Six of the 12 TLDs were then randomly selected for re-use. Three were placed in the phantom in the region of the thyroid and a pantomogram again taken. The procedure was repeated for a cephalogram and the TLDs again read. In all cases the readings of each group of 3 TLDs did not vary by more than 10% on either side of the mean readings. The TLD readings were then converted by means of a conversion factor to actual dose measurements. The doses to left and right eyes and to the thyroid were respectively found to be 0,0151, 0,0222 & 0,0896 mSv for the pantomogram and 0,0351, 0,0183 & 0,0177 mSv for the cephalogram--an almost insignificant dose in terms of the "background equivalent" concept.

Cephalometry↗
PubMedDate not supplied

Determination of elemental composition of substance lost following wear of all-ceramic materials.

PURPOSE: The aim of this study was to test the possible elemental release of four different all-ceramic materials in a wear machine to predict results about their long-term behavior in the oral environment. MATERIALS AND METHODS: Four different all-ceramic materials with different chemical compositions were selected for the wear testing. A total of 20 cylindric samples, five for each ceramic group, were prepared according to the manufacturers' instructions. These were subjected to two-body wear testing in an artificial saliva medium under a covered unit with a computer-operated wear machine. The artificial saliva solutions for each material were analyzed for the determination of amounts of sodium, potassium, calcium, magnesium, and lithium elements released from the glass-ceramic materials. The differences between and within groups were statistically analyzed with a one-way ANOVA, followed by Duncan tests. RESULTS: The statistical analyses revealed no significant differences among Na, K, Ca, or Mg levels (P > .05) released from the leucite-reinforced groups, while there was a significant (P < .05) increase in Li release from the lithium disilicate group. CONCLUSION: Considerable element release to the artifical saliva medium was demonstrated in short-term wear testing. The lithia-based ceramic was more prone to Li release when compared with other elements and materials.

Aluminum Silicates↗
PubMedDate not supplied

Influence of surface conditions and silane agent on the bond of resin to IPS Empress 2 ceramic.

PURPOSE: The aim of this study was to evaluate the effect of different ceramic surface treatments on the tensile bond strength between IPS Empress 2 ceramic framework and Rely X adhesive resin cement, with or without the application of a silane coupling agent. MATERIALS AND METHODS: One hundred twenty disks were made, embedded in resin, and randomly divided into six groups: group 1 = sandblasting (100 microm), no silanation; group 2 = sandblasting (100 microm), silane treatment; group 3 = sandblasting (50 microm), no silanation; group 4 = sandblasting (50 microm), silane treatment; group 5 = hydrofluoric acid etching, no silanation; and group 6 = hydrofluoric acid etching, silane treatment. The disks were bonded into pairs with adhesive resin cement. All samples were stored in distilled water at 37 degrees C for 24 hours and then thermocycled. The samples were submitted to tensile testing. RESULTS: The use of silane improved the bond strength in relation to the groups in which silane was not applied (P < .05). The most effective surface treatment was etching with 10% hydrofluoric acid, both with (25.6 MPa) and without silane application (16.4 MPa); these values showed a statistically significant difference compared to sandblasting with 50- and 100-microm Al2O3. Sandblasting with 50-microm Al2O3, with (11.8 MPa) and without silane (5.4 MPa), demonstrated significantly higher tensile bond strength than sandblasting with 100-microm Al2O3, with (8.3 MPa) and without silane (3.8 MPa). CONCLUSION: Combined application of 10% hydrofluoric acid and silane enhanced the bond strength between the IPS Empress 2 ceramic framework and resin agent.

Acid Etching, Dental↗
PubMed2002

[Polarization characteristic of LiF polarizer at UV-VUV spectral range].

A method of determinating the degree of polarization of grating, polarizer and analyzer at some wavelength is introduced. The degrees of polarization of LiF polarizers were determined with 1 m Seya-Namioka monochromator by this way. The source is hollow cathode source. The degrees of polarization of two, four and eight LiF plates were measured at 120, 149.2, 174.2, 214.2, 235.0, 300 and 340 nm. The theoretical and experimental reults were consistent within the error. The angular extinction dependence of the LiF polarizer and analyzer was measured at VUV wavelengths. The results showed the expected cosine square dependence, so a high degree of polarization was indeed produced. Also, the LiF polarizer was used to measure the polarization characters of the Al + MgF2 reflectance and the absolute grating efficiency at both parallel and perpendicular to their incident plane.

Aluminum↗
PubMed2001

[An IR study of ion solvation and association of lithium perchlorate in some organic solvents].

The infrared (IR) spectra of propylene carbonate (PC), gamma-butyrolactone (BL) and diethyl carbonate (DEC) in the presence of LiClO4 have been investigated. It is shown that the interactions between Li+ and these solvents occur on the oxygen atoms of the carbonyl of solvent molecules. On the other hand, spectral curve fitting for band shape of perchlorate anion shows the presence of ion association in these solutions.

4-Butyrolactone↗
PubMed2003

[Research on IR spectra of Li-Mn spinel].

The infrared spectra of Li-Mn-spinel were studied in this paper. As the structure of Li-Mn-spinel was classified as Fd3m space group, the lithium ions (Li(I)) occupy tetrahedral sites (8a sites) and manganese ions (Mn(IV) or Mn(III)) occupy octahedral sites (16d sites). The internal relationship between vibration modes and infrared activity was discussed based on the knowledge of group theory. The experimental IR spectra of Li-Mn-spinel were presented also. By theoretical analysis, we concluded that the bands at 618.6 and 501.5 cm-1 resulted from anti-symmetric stretching vibration of Mn(IV)-O and Mn (III)-O in Li-Mn-spinel crystal (Mn(IV)O6 and Mn(III)O6 octahedron), respectively, the weak band at 1,124 cm-1 resulted from anti-symmetric stretching vibration of Li-O in the spinel (LiO4 tetrahedron), and other possible bands at wave number lower than 400 cm-1 were not detected in the range from 400 to 4,000 cm-1. The reliability of conclusions was proved by IR spectra of both the Li-Mn-spinel and doped Li-Mn-spinel.

Crystallization↗
PubMed2003

Effectiveness and outcome predictors of long-term lithium prophylaxis in unipolar major depressive disorder.

OBJECTIVE: To determine the effectiveness of lithium prophylaxis in unipolar major depressive disorder (MDD) and to identify predictors of outcome including comedication. METHODS: In this long-term naturalistic study, clinical data from 55 patients with MDD (DSM-III-R) were collected prospectively in an outpatient clinic specializing in the treatment of affective disorders. OUTCOME MEASURES: Change in hospital admission rate (number and duration) during prophylaxis compared with the period before prophylaxis, Morbidity-Index during prophylaxis and time to first recurrence after initiation of lithium treatment. RESULTS: During an average follow-up period of 6.7 years, a significant decline in the number of days spent in hospital (p<0.001; 52 d/yr less; 95; CI 31-73 d) and a low Morbidity-Index (mean 0.07) was observed. Only in 6 patients did medication have to be changed because of side-effects (n=4) or a lack of efficacy (n=2). None of the independent variables we analyzed proved to be important in predicting the outcome of lithium prophylaxis. Comedication was necessary in 21 patients. The overall outcome of their prophylactic treatment, however, did not differ from the group that did not receive comedication in the symptom-free intervals. CONCLUSIONS: The results of this study, with its long observation period and the inclusion of comedication as a confounding variable, indicate that lithium is a potent prophylactic agent for unipolar MDD in a naturalistic setting. In contrast to the findings of others, age was not associated with the outcome of prophylaxis, and latency did not predict outcome. Contrary to doubts that have been raised in recent years with regard to the effectiveness of lithium in everyday clinical practice, lithium appears to be a safe and potent alternative to antidepressants.

Adult↗
PubMed2003

Specificity and potential mechanism of sulfatide deficiency in Alzheimer's disease: an electrospray ionization mass spectrometric study.

Recently, we have demonstrated that sulfatide content was substantially depleted in post-mortem brain samples from subjects with very mild Alzheimer's disease (AD) relative to age-matched controls. However, it is unknown if the observed sulfatide deficiency is AD-specific and what mechanism(s) lead to this depletion. By exploiting the advantages of electrospray ionization mass spectrometry techniques, we examined the specificity and a potential mechanism of sulfatide deficiency in AD in the study. In contrast to the sulfatide depletion observed in AD, it was found that the sulfatide content in post-mortem brain samples from subjects with Parkinson's disease and dementia with Lewy bodies was either higher than or comparable to that observed from controls, respectively, suggesting that sulfatide deficiency is likely specific to AD. Examination of lipid alterations in cultured embryonic rat brain oligodendrocytes treated with amyloid-beta peptide demonstrated that there was no alteration in sulfatide content up to a 24-hr interval after amyloid-beta addition/treatment. However, there were significant decreases in plasmenylethanolamine and increases in sphingomyelin content in the same study. These findings suggest that sulfatide deficiency in AD is unlikely mediated directly by amyloid-beta peptide accumulation. Thus, these results illustrate the specificity of sulfatide deficiency in AD and exclude amyloid-beta accumulation as a factor directly contributing to sulfatide deficiency in AD.

Aged↗
PubMed2003

Carbon dioxide scrubbing capabilities of two new nonpowered technologies.

Current guidance for survivors aboard a disabled submarine (DISSUB) recommends the use of the "stir-and-fan" method of carbon dioxide (CO2) scrubbing in which the contents of canisters of lithium hydroxide (LiOH) are dispersed onto horizontal surfaces. This technique is objectionable because it releases large quantities of fine, caustic LiOH dust and it utilizes LiOH inefficiently. This report presents the results of laboratory studies of the CO2 scrubbing capabilities of two new products that might improve on "stir-and-fan": the Battelle Curtain (BC) and the Micropore Reactive Plastic Curtain (RPC). Experiments took place within a sealed hyperbaric chamber. CO2 was added to the chamber at a known mass flow that reproduced what might be encountered in a "worst-case" DISSUB scenario. Natural convection alone circulated gas within the chamber. The mass of BCs or RPCs necessary to limit CO2 to 3% for about 2 days was determined. The total scrubbing capacity (mass of CO2 scrubbed per unit mass of agent) of the BC was 0.756 +/- 0.012 (mean +/- SD), and the comparable value for the RPC was 0.808 +/- 0.007. Both products provided a scrubbing capacity that is close to the stoichiometric limit of the reaction (0.919). Neither product released sufficient caustic dust to prevent handling by a trained individual wearing no personal protective equipment.

Air Conditioning↗
PubMed2003

Comparison of radiation levels from computed tomography and conventional dental radiographs.

BACKGROUND: With the increasing use of computed tomography (CT) in oral diagnosis and treatment planning, concern has been expressed about the high levels of radiation used, and the associated risks. OBJECTIVES: The purpose of this study was to compare the radiation doses of facial CT scans with the radiation doses taking a lateral cephalometric radiograph, a panoramic radiograph (OPG), an occlusal film, and an intra-oral periapical radiograph. METHODS: An Alderson-Rando anthropomorphic phantom head was used for the analysis. Thirty-six lithium fluoride thermoluminescent dosimeters were placed in the phantom head in locations representing radiosensitive sites. Standard facial CT scans and conventional radiographs (lateral cephalometric, OPG, maxillary occlusal, intra-oral periapical) were then taken of the phantom head. RESULTS: The following radiation doses were measured: maxillo-mandibular CT scan, 2.1 mSv; maxillary CT scan, 1.40 mSv; mandibular CT scan, 1.32 mSv; lateral cephalometric radiograph, 0.005 mSv; OPG, 0.010 mSv; maxillary occlusal, 0.007 mSv; intra-oral periapical radiograph, 0.005 mSv. CONCLUSIONS: CT scans produce significantly more ionising radiation than conventional radiographs. This factor should be taken into account when considering a CT scan as an alternative to a survey with conventional radiographs. While CT scans offer many advantages over conventional radiography the high radiation dose to patients, and the cost of this procedure should be considered.

Brain↗
PubMedDate not supplied

Clinical evaluation of marginal fit of a new experimental all-ceramic system before and after cementation.

PURPOSE: The purpose of this in vivo study was to evaluate the marginal fit of inlay and crown abutments for fixed partial dentures (FPD) made from a new all-ceramic material. MATERIALS AND METHODS: Sixteen patients were selected for the study. After abutment preparation, impressions were made and master dies were fabricated. FPDs were made with the experimental heat-pressed lithium-disilicate glass-ceramic following the manufacturer's recommendations. At random, one abutment of each FPD was chosen for evaluation (11 crown and 8 inlay abutments). Impressions of the restoration margins were taken before and after adhesive cementation. Replicas of the abutment margins were investigated by SEM. The marginal discrepancies were evaluated in sections of 200 microm. The highest value of each section was applied, and the mean of all sections of each replica was used for statistical analysis. RESULTS: The median of the marginal discrepancies before cementation was 96 microm for crowns and 89 microm for inlays. After adhesive cementation, the median increased for crowns to 130 microm and for inlays to 92 microm, a significant increase in the marginal discrepancies caused by cementation for crowns, but not for inlays. There were no significant differences between the discrepancies of crowns and inlays before or after cementation. CONCLUSION: The maximum marginal discrepancies found in this study were between 50 and 265 microm before cementation and between 70 and 285 microm after cementation, with no significant differences between the means for crowns and inlays. Adhesive cementation caused a significant increase of the marginal discrepancies for crown, but not inlay, abutments.

Cementation↗