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The early social environment of premature and fullterm infants.

The behaviors of 10 mothers of prematures and 29 mothers of fullterms were compared from 7-h observations made in the home when the infants were 2, 3, 4 and 5 weeks post-term. The observation day was divided into four mutually exclusive interactional contexts that together made up the total day: feeding time, changing or bathing time, time when the mother and infant were in physical contact but the mother was not caretaking, and time the infant was alone. Measures of ten maternal behaviors were also compared: moving, rocking, patting, caressing, talking, looking, engaging in vis-a-vis with the baby, holding or carrying, smiling or laughing, and stimulating the baby to suck. Mothers of prematures left their infants alone more and changed them less than mothers of fullterms. In addition, mothers of prematures moved their infants less often, talked to their infants less, looked at their infants less, and held their infants less. These results indicate that, over the 7-h day, prematures receive markedly less stimulation than fullterms. Since the neurobehavioral characteristics of premature and fullterm infants are known to differ, it is suggested that these differences in maternal behaviors may be in response to infant cues and appropriate for the infants.

Adolescent↗

Prevalence of selected maternal and infant characteristics, Pregnancy Risk Assessment Monitoring System (PRAMS), 1997.

PROBLEM/CONDITION: Certain maternal behaviors and experiences (e.g., unintended pregnancy, late entry into prenatal care, smoking cigarettes during pregnancy, and physical abuse during pregnancy) might be related to adverse reproductive outcomes (e.g., low birthweight, infant morbidity and mortality, and maternal morbidity). Information on the prevalence of these behaviors and experiences is needed to monitor trends over time, to increase understanding of maternal behaviors and experiences and their relation to perinatal and maternal outcomes, and to develop and assess programs and policies designed to reduce these adverse outcomes among pregnant women and their infants. REPORTING PERIOD: From 1993 through 1997. DESCRIPTION OF SYSTEM: The Pregnancy Risk Assessment Monitoring System (PRAMS) is a CDC-developed, ongoing, state- and population-based surveillance system designed to collect information on self-reported maternal behaviors and experiences that occur before, during, and shortly after pregnancy among women who deliver a live-born infant. A 14-page questionnaire is mailed to a sample of mothers that is randomly selected from state birth certificate records. The questionnaire is followed by two additional mailings and follow-up with nonresponders by telephone. Responses are accumulated during the calendar year, combined with birth certificate data, and then weighted to be representative of all mothers who had a live-born infant in the state. Data for 1997 from 13 states were examined. RESULTS: The prevalence of unintended pregnancy resulting in a live-born infant ranged from 33.9% to 50.0% in the 13 states. From 1993 through 1997, data from Georgia demonstrated a significant decreasing trend (p = .01) in unintended pregnancy, whereas this trend significantly increased in New York (p = .03). In most states, women who were younger (aged <20 years), had less than 12 years of education, were black, and received Medicaid were more likely to report an unintended pregnancy. In 1997, 16.6%-30.7% of women entered prenatal care after the first 3 months of pregnancy. In most states, women who were younger (aged <20 years), black, had lower levels of education, and received Medicaid were more likely to enter prenatal care late or not at all. Georgia and Washington experienced significant decreasing trends in smoking during pregnancy. Across the 13 states, 11.0%-23.9% of women reported smoking during pregnancy. In seven of 13 states, white women were more likely to smoke during pregnancy than black women. In eight of 13 states, smoking was significantly more prevalent among women who delivered a low birthweight infant than women who delivered a normal weight infant. In seven states, the proportion of women who initiated breast-feeding significantly increased from 1993 through 1997; the prevalence in 1997 ranged from 48.1% to 86.8%. Women who were most likely to breast-feed were older, more educated, white, and did not receive Medicaid. The state-specific prevalence of physical abuse during pregnancy by a husband or partner ranged from 2.4% to 5.6%. In most of these states, women who had less than a high school education and women who received Medicaid were more likely to report having been abused. INTERPRETATION: Findings indicate that many women report high-risk behaviors or experience high-risk conditions before, during, and shortly after pregnancy. Furthermore, several specific groups of women were significantly more likely to report these behaviors or experience these conditions. In many states, women who were younger, less educated, and Medicaid recipients were more likely to experience unintended pregnancy, enter prenatal care after the first 3 months of pregnancy or not at all, smoke during the last 3 months of pregnancy, and never initiate breast-feeding. PUBLIC HEALTH ACTION: These findings are useful for state agencies to monitor trends in behaviors and experiences and to design public health programs and policies that addre

Adult↗

Evaluation of a two-generation reproduction toxicity study adding endpoints to detect endocrine disrupting activity using lindane.

A two-generation reproduction toxicity study in rats adding extra endpoints to detect endocrine disrupting activity was conducted using lindane by dietary administration at 0, 10, 60, and 300 ppm, for investigation of its utility. The extra endpoints included anogenital distance (AGD), nipple development, sexual maturation (vaginal opening and preputial separation), estrous cycle, spermatogenesis, sex organ weights, and blood hormone concentrations (thyroid and sex hormones). F1 offspring were examined for emotionality (open field test), motor coordination (rotarod test), as well as learning and memory (pole-climbing test). Hepatic drug-metabolizing enzyme activities were also measured. The results revealed general toxicological effects on parental animals, influence on reproductive function, and altered development of offspring; however, they did not demonstrate any distinct changes in the extra endpoints for detection of endocrine disrupting activity. Adult toxicity was observed in both F0 and F1 animals, including suppressed body weight gain and reduced food consumption in both sexes, and deaths of females at 300 ppm. Convulsions and irritability were observed during the perinatal period in pregnant F1 females given 300 ppm. Pathological examination revealed increased liver weights and centrilobular hepatocellular hypertrophy in both sexes and generations at 10 or 60 ppm and above; in addition, increased kidney weights and increased hyaline droplets in the proximal tubule epithelium, and basophilic renal tubules in males were noted at 10 ppm and above. Pituitary weights were decreased in F0 females and in F1 males and females and adrenal weights were increased in F1 males and females at 300 ppm; however, no histological changes were observed, and manifestations suggesting endocrine disrupting activity related to these changes were lacking. Hypertrophy of the thyroid follicular epithelium in F0 females at 300 ppm and in F1 males at 60 and 300 ppm, and decreases in T3 and/or T4 in both sexes and generations at 300 ppm were presumed to be secondary changes associated with the induction of hepatic drug-metabolizing enzymes. Blood hormone analysis revealed no changes in sex hormones attributable to lindane in males or females. Hepatic drug-metabolizing enzyme activities were increased dose-dependently from 10 ppm in both sexes and generations, with the rise in BROD activity being the most prominent. There were also increases in MROD, EROD, T-6beta-OH, and T4-UDP-GT activities (BROD >> EROD > MROD, T-6beta-OH, T4-UDP-GT). This suggests that while lindane most strongly induces CYP2B, it also upregulates a number of other drug metabolizing enzymes, such as CYP1A, CYP3A, and UDP-GT. As for effects on reproductive function, lack of maternal behavior, including lactation and retrieval behavior, and consequent total litter loss were observed in F1 dams at 300 ppm. There were no effects of lindane on the estrous cycle, spermatogenesis, mating, fertility, pregnancy, or parturition. Neonatal toxicity was observed in both sexes and generations, including suppressed body weight gain at 60 and 300 ppm, and decreased thymus and spleen weights without histological change at 300 ppm. The postnatal survival rate in F2 offspring was decreased due to lack of maternal behavior in dams at 300 ppm.

Administration, Oral↗

Infant temperament and noncompliant behavior at four years: a longitudinal study.

Noncompliant behavior in early childhood (i.e., exaggerated, but age-appropriate negativism) has been inadequately studied. This longitudinal study explores preschool temperament and maternal behavior as current correlates of negativistic behavior at age 4, and early maternal characteristics and infant temperament as etiological variables in the production of negativism. Longitudinal evidence was collected from 62 mother-child dyads, representative of all social classes. This evidence included home observations of maternal and child behavior at 4 years, a temperament questionnaire at 4 years, a questionnaire about infant temperament, and observational and interview measures at various stages from birth to 3 years, of infant behavior and maternal characteristics. The findings suggest that infant and childhood temperament (especially the degree of infant fussiness) are highly predictive of physical noncompliance, and that physical noncompliance is unrelated to maternal or demographic variables. A combination of maternal variables including warmth, closeness, and verbal directiveness, both positive and negative, as well as child variables, predict verbal noncompliance. Demographic variables, temperament, and maternal attitudes predict passive noncompliance. Noncompliance is viewed as a complex phenomenon in which constitutional and environmental variables interact.

Child Behavior Disorders↗

The effect of cholecystokinin-octapeptide on food intake and consummatory behavior in lactating rats.

Rats are less sensitive to the satiating effect of CCK-8 during some reproductive states such as estrus and proestrus, and in ovariectomized rats following the administration of estradiol and progesterone. The sensitivity of rats to CCK-8's effect on food intake decreases as lactation progresses. During lactation, prolactin and progesterone levels are elevated. Implantation of ectopic pituitaries increases prolactin levels in males and females as well as progesterone levels in females. To evaluate whether or not prolactin elevation modifies CCK's effect on feeding, we studied the effect of CCK-8 on food intake during the early dark cycle in male and female rats implanted with ectopic pituitaries. As previously demonstrated, prolactin levels were elevated in both male and female pituitary-implanted rats and progesterone levels were elevated in the female rats. CCK-8 inhibited food intake in sham-operated male rats, but did not reliably decrease early dark cycle food intake in sham-operated or pituitary-implanted female rats or pituitary-implanted male rats. Thus an elevation in prolactin levels does not appear to modify the effect of CCK-8 on food intake in female rats. We also evaluated the effect of CCK on consummatory and maternal behavior in lactating rats. CCK-8 altered the meal patterns of lactating rats primarily by decreasing the rate of food consumption and increasing the latency to the first meal. The latency to the first meal of rats receiving CCK was increased during early and mid-lactation and the PW period, but not during late lactation compared to that of the saline-injected rats. CCK-8 did not modulate any of the maternal behaviors studied.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

Complete maternal deprivation affects social, but not spatial, learning in adult rats.

The effects of maternal deprivation on learning of social and spatial tasks were investigated in female adult rats. Pups were reared artificially and received "lickinglike" tactile stimulation (AR animals) or were reared with their mothers (MR animals). In adulthood, subjects were tested on paradigms of spatial learning and on paradigms involving learning of social cues. Results showed that maternal deprivation did not affect performance on spatial learning, but it did impair performance on the three social learning tasks. The AR animals made no distinction between a new and a previously presented juvenile conspecific. AR animals also responded less rapidly than MR animals at test for maternal behavior 2 weeks after a postpartum experience with pups. Finally, AR animals did not develop a preference for a food previously eaten by a familiar conspecific whereas MR animals did. This study indicates that animals reared without mother and siblings show no deficits in spatial tasks while showing consistent deficits in learning involving social interactions.

Animals↗

Maternal factors and monoamine changes in stress-resilient and susceptible mice: cross-fostering effects.

Genetic factors influence stressor-provoked monoamine changes associated with anxiety and depression, but such effects might be moderated by early life experiences. To assess the contribution of maternal influences in determining adult brain monoamine responses to a stressor, strains of mice that were either stressor-reactive or -resilient (BALB/cByJ and C57BL/6ByJ, respectively) were assessed as a function of whether they were raising their biological offspring or those of the other strain. As adults, offspring were assessed with respect to stressor-provoked plasma corticosterone elevations and monoamine variations within discrete stressor-sensitive brain regions. BALB/cByJ mice demonstrated poorer maternal behaviors than C57BL/6ByJ dams, irrespective of the pups being raised. In response to a noise stressor, BALB/cByJ mice exhibited higher plasma corticosterone levels and elevated monoamine turnover in several limbic and hypothalamic sites. The stressor-provoked corticosterone increase in BALB/cByJ mice was diminished among males (but not females) raised by a C57BL/6ByJ dam. Moreover, increased prefrontal cortical dopamine utilization was attenuated among BALB/cByJ mice raised by a C57BL/6ByJ dam. These effects were asymmetrical as a C57BL/6ByJ mice raised by a BALB/cByJ dam did not exhibit increased stressor reactivity. It appears that stressors influence multiple neurochemical systems that have been implicated in anxiety and affective disorders. Although monoamine variations were largely determined by genetic factors, maternal influences contributed to stressor-elicited neurochemical changes in some regions, particularly dopamine activation within the prefrontal cortex.

Animals↗

Neonatal behaviors associated with ultrasonic vocalizations in mice (mus musculus): A slow-motion analysis.

Infant rodents emit ultrasonic vocalizations (USVs) when isolated from the mother and littermates. While USVs reportedly promote maternal behavior, their communicatory role is still controversial. We performed a detailed behavioral characterization in 7-day-old CD-1 Swiss mice to describe (a) behavioral patterns accompanying or immediately preceding or following the USV and (b) how selected USV features are modulated according to the behavioral pattern being performed. The present results represent an ethogram of mouse pup behavior in close temporal proximity to USV, providing further information to evaluate the functional role of USVs in the framework of the mother-offspring relationship. A significant increase of head rising behavior immediately prior to the USV and a behavioral activation displayed by pups in conjunction with it suggest that pups behave in a way that may favor interaction with their mothers.

Animal Communication↗

Depression, partnership, social support, and parenting: interaction of maternal factors with behavioral problems of the child.

BACKGROUND: Children of depressed mothers are at elevated risk to develop behavioral problems. Besides maternal depression, other risk factors like interpersonal functioning, are discussed. The object of this study was to investigate the predictive value of the maternal variables depression, partnership, social support, and parenting for internal and external behavioral problems of the children. METHODS: A total of 100 mothers and their children who participated in a German mother-child rehabilitation program were interviewed by questionnaire. Structural equation modeling was conducted in order to determine the predictive value of the mentioned maternal variables on behavioral problems of the children. RESULTS: Behavioral problems of the children can be predicted by the parenting and the partnership of the mothers. Mothers with more parenting difficulties and with less containment with partnership report more behavioral problems of their children. Depression and social support do indirectly predict the child's problems. LIMITATIONS: The data is from a cross-sectional sample. Therefore, path models do not demonstrate causation. All information is based upon maternal report. CONCLUSIONS: These findings suggest the need for intervention programs which focus on the parenting and the partnership of the mothers. More research with independent assessment is needed.

Adult↗

Effects of Tennessee Medicaid managed care on obstetrical care and birth outcomes.

A comparative study was conducted in two neighboring states, Tennessee and North Carolina, to determine whether Medicaid managed care (implemented in Tennessee as TennCare) affected prenatal care, care patterns at labor-delivery, and birth outcomes. A pre- and post-design coupled with a difference-in-difference approach--using North Carolina as a control--was used to assess TennCare's effects for all births and for three categories of high-risk mothers (under age eighteen, unwed, or living in high poverty areas). Data from 328,296 singleton births in birth files and matched birth-death files for 1993 and 1995 in both states were used to analyze a number of variables related to maternal behavior during pregnancy, utilization of care before and after labor-delivery, patterns of obstetrical care at delivery, and birth outcomes. Under TennCare, Tennessee mothers were relatively more likely to obtain no prenatal care or to wait and initiate third trimester care as compared to those in North Carolina. Relative utilization of specific prenatal procedures declined, Apgar scores fell very slightly, and birth abnormalities increased in the poverty subsample. TennCare had no significant effect on infant mortality. Utilization reductions in obstetrical services were achieved with apparent spillovers to non-TennCare births, but without adverse effects overall. TennCare was neither a panacea nor an unmitigated disaster. It is a model worth examining, but not uncritically.

Female↗

Neonatal social isolation alters both maternal and pup behaviors in rats.

The development of emotional behavior is dependent on the early experiences of the infant and the quality of maternal care. In these experiments, the effects of social isolation during the preweaning period on both pup behavior and maternal responsivity were examined. In the first study, the number of ultrasonic vocalizations (USVs) emitted after brief maternal separation was measured in neonatal rats with differing histories of social isolation. The social isolation procedure consisted of 5 days of daily separation from the dam and littermates for either 3 or 6 hr. At both ages tested, socially isolated pups vocalized significantly less than control pups. In the second study, the effects of prior isolation either daily for 5 previous days (Chronic Isolation) or for 4 hr prior to testing (Acute Isolation) were examined in a T-maze choice test. Pup vocalizations in the presence of the dam and dams' maternal behavior were assessed. When the dam was confined to the start box or during the maternal free access period, both Chronic and Acute Isolates vocalized less than pups that had never left the home nest. Dams spent more time with and licked and groomed more frequently and for a longer time both Chronic and Acute Isolates compared to pups that had always been with dams in the home nest. These results suggest that early isolation experience can alter subsequent responses to separation stress in neonatal rats and that maternal behavior is sensitive to the prior experiences of offspring.

Animals↗

Correlates of maternal directiveness with children who are developmentally delayed.

Interactions between 25 mothers and their children with developmental delays were correlated to determine the relationships between maternal directiveness and a) maternal behavior regarded as developmentally facilitative, b) maternal intrusiveness, and c) child developmental competence and behavioral engagement. The findings, many of which challenge common conceptions about the nature and role of maternal directiveness, are discussed in relation to these conceptions and to the potential role of directiveness in the development of children with handicaps.

Adult↗

Effects of Shiga toxin 2 on lethality, fetuses, delivery, and puerperal behavior in pregnant mice.

Shiga toxin 2 (Stx2) is produced by enterohemorrhagic Escherichia coli (EHEC) and is known as the major virulence factor of EHEC. The aim of this study was to evaluate the effects of Stx2 on (i) maternal lethality, (ii) fetuses, (iii) delivery period, and (iv) maternal behavior after delivery. Timed pregnant ICR mice were injected intravenously with Stx2 on day 5 of pregnancy (early stage) or on day 15 (late stage). In early-stage experiments, the number of normal fetuses of mice injected with Stx2 was significantly lower than that of control mice. In late-stage experiments, mothers injected with Stx2 delivered normal numbers of neonates, but could not take care of them. The lethal doses of Stx2 were not different for pregnant and nonpregnant female mice at either stage. We conclude that Stx2 is toxic to the fetus in early pregnancy and affects maternal puerperal behavior in late pregnancy.

Animals↗

Reproductive experience increases prolactin responsiveness in the medial preoptic area and arcuate nucleus of female rats.

The experience of pregnancy plus lactation produces long-term enhancements in maternal behavior as well as reduced secretion of prolactin, a key hormone for the initial establishment of maternal care. Given that prolactin acts centrally to induce maternal care as well as regulate its own secretion, we tested whether prolactin receptors in brain regions known to regulate behavioral and neuroendocrine processes were up-regulated and more responsive to prolactin in reproductively experienced females. Diestrous primiparous (8 wk after weaning) and age-matched virgin rats were treated with 250 microg ovine prolactin sc or vehicle and the brains collected 2 h later for measurement of mRNA for genes involved in prolactin signaling. Reproductively experienced rats had lower serum prolactin concentrations, compared with virgin rats, suggesting enhanced prolactin feedback on the arcuate neurons regulating prolactin secretion. In the medial preoptic area and arcuate nucleus (regions involved in regulating maternal behavior and prolactin secretion, respectively), the level of long-form prolactin receptor mRNA was higher in primiparous rats, and prolactin treatment induced a further increase in receptor expression in these animals. In the same regions, suppressors of cytokine signaling-1 and -3 mRNA levels were also markedly increased after prolactin treatment in reproductively experienced but not virgin rats. These results support the idea that reproductive experience increases central prolactin responsiveness. The induction of prolactin receptors and enhanced prolactin responsiveness as a result of pregnancy and lactation may help account for the retention of maternal behavior and shifts in prolactin secretion in reproductively experienced females.

Animals↗

Relationship of maternal parenting behaviors to preschool children's temperament.

Mothers of 182 preschool nursery school children rated their own parenting responses on a "Parent's Report" questionnaire. At the same time the mothers responded to the "Behavior Style Questionnaire" (BSQ) from which scores were determined for nine categories of temperament. On the basis of category scores the children were grouped into one of five temperament clusters i.e. easy, difficult, slow to warm up, high intermediate, low intermediate. The children's membership in BSQ clusters was independent of sex, age, birth order, and mothers employment status but there was a significantly higher ratio of "easy" children from higher socioeconomic classes I and II. Mothers of children grouped in either the "difficult" or "slow to warmup"clusters were more likely to use "guilt inducing" and "temper-detachment" parenting styles than mothers of children grouped in the "easy" cluster.

Child↗

Effects of maternal hormones on 'timidity' and attraction to pup-related odors in female rats.

The present study was designed to determine whether changes in 'emotionality' and responses to odorants that occur in the postpartum rat are due to the same configuration of hormones that facilitate the expression of maternal behavior. Ovariectomized females were implanted with silastic capsules containing progesterone and estradiol or cholesterol for a 19-21 day period and were tested 1 or 7 days later for emergence behavior, ambulation in the open-field and responses to nesting-material containing different odorants. All females were given two tests in which clean nest-material was presented and two with lactating nest material. In comparison to cholesterol animals hormonally primed animals emerged more rapidly into the open-field, crossed more squares while in the field and spent more time around the odor stimulus. Moreover, hormonally primed animals crossed more squares in the region around the odor source when lactating nest odor was present than when it was not and, under the lactating nest odor condition, crossed more squares in the stimulus region than did any other group. This study suggests that the Bridges' regimen of progesterone and estradiol reduces general 'timidity,' reflected in open-field performance, and increases attraction to pup-related odors. These hormone-induced behavioral changes may well contribute to the heightened maternal responsiveness also produced by these hormones.

Animals↗

The actions of prolactin in the brain during pregnancy and lactation.

The vital role played by prolactin during pregnancy and lactation is emphasized by the physiological adaptations that occur in the mother to maintain a prolonged state of hyperprolactinemia. In many species the placenta provides a source of lactogenic hormones in the circulation, ensuring the continued presence of a hormone capable of activating the prolactin receptor throughout pregnancy. In addition, the tuberoinfundibular dopamine neurons, which normally maintain a tonic inhibitory influence over prolactin secretion, show a reduced ability to respond to prolactin during late pregnancy and lactation, allowing high levels of prolactin to be maintained unopposed by a regulatory feedback mechanisms. There is clear evidence that systemic prolactin gains access to the cerebrospinal fluid, from where it can diffuse to numerous brain regions. Prolactin receptors are expressed in several hypothalamic nuclei, including the medial preoptic and arcuate nuclei, and we have observed marked increases in expression of prolactin receptors in these nuclei during lactation. Moreover, a number of hypothalamic nuclei, including the paraventricular, supraoptic and ventromedial nuclei, in which prolactin receptors were not detected in diestrous rats, were found to express significant amounts of prolactin receptor during lactation. These observations have important implications for the variety of documented actions of prolactin on the brain. Prolactin has been reported to influence numerous brain functions, including maternal behavior, feeding and appetite, oxytocin secretion, and ACTH secretion in response to stress. In light of the high circulating levels of prolactin during pregnancy and lactation and the increased expression of prolactin receptors in the hypothalamus, many of these effects of prolactin may be enhanced or exaggerated during lactation. Hence, prolactin may be a key player in the coordination of neuroendocrine and behavioral adaptations of the maternal brain.

Animals↗