Platelet count and thrombelastographic maximal amplitude in experimental thrombocythemias.
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In a previous work, we have compared two automated techniques for platelet-rich plasma using a thrombocounter or whole blood with the continuous flow instrument Auto-analyzer (AA). More recently, we have tested two instruments using whole blood: the first one, fully automated: Coulter S+; and the other one, semi-automated: Clay-Adams. In the present work, these 4 methods are compared to the phase contrast microscope technique, used as reference. The coefficients of variation ranged between 2 and 13 per cent. The coefficients of correlation between the different methods were between 0.92 and 0.96. Platelet distribution curves for platelet volumes obtained in parallel with platelet-rich plasma and with whole blood in 30 controls, show that platelet population are not significantly different. These instruments count particles and, therefore, necessitate a calibration made by means of platelet standards. Different platelet standards have been studied: these containing latex particles seem to give better results than suspensions of human or animal platelets.
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The estimation of the results of automatic calculations of the blood platelets was made. The probability of the false thrombocytopenia data obtaining has been noted. The use of this method for the preliminary calculation of the platelets number in stained blood sample and revealing the cases with false thrombocytopenia has been suggested.
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