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At least 865 records · Page 48Linked to original sources
PubMed1997

Identification of lysyl oxidase and TRAMP as the major proteins in dissociative extracts of the demineralized collagen matrix of porcine dentine.

Carbonated apatite (dahllite) is formed within and between collagen fibrils in the mineralization of connective tissues. However, the mechanism of crystal nucleation at these sites has not been resolved. To identify non-collagenous proteins that may be involved in the nucleation process we have utilized a dissociative extraction procedure to isolate proteins associated non-covalently with the de-mineralized collagen matrix of dentine isolated from tooth roots of adult porcine incisors. Following extraction of dentine fragments with 4M GuHCl (G1-extract) and 0.5M EDTA (E-extract), de-mineralized collagen matrix-associated proteins were isolated with a second series of extractions with 4M GuHCl (G2-extract). Analysis of the G2-extracts on SDS-PAGE revealed two major 32 kDa and 24 kDa protein bands, comprising > 80% of the extracted non-collagenous proteins. The 32 kDa protein was purified by FPLC on hydroxyapatite and Mono Q resins, followed by HPLC reverse-phase chromatography. Small amounts of 26 kDa and 6 kDa proteins, which appear to represent proteolytically processed, disulphide-linked fragments of the 32 kDa protein, co-eluted with the major protein. The 32 kDa protein was identified as lysyl oxidase from amino acid sequence analysis of a 13 kDa CNBr peptide obtained from protein purified by preparative electrophoresis on SDS-PAGE. Fractionation of the 24 kDa protein on FPLC Mono Q resin generated < 5 closely eluting protein peaks. The proteins from these peaks were similar in size, staining properties, amino acid composition and CNBr digestion patterns. Each protein was immunoreactive with antibodies raised against a tyrosine-rich acidic matrix protein (TRAMP), reported previously to co-purify with lysyl oxidase. These studies, therefore, show that lysyl oxidase, which is important in collagen cross-link formation, and proteins with properties of TRAMP, a protein that can modulate collagen fibrillogenesis, are the major proteins in dissociative extracts of de-mineralized porcine dentine.

Alkylation↗
PubMed1997

Characterization of cementum derived growth factor as an insulin-like growth factor-I like molecule.

Cementum is the thin calcified outer layer through which tooth-root surfaces are anchored to soft periodontal connective tissues. A variety of growth factors and adhesion molecules are sequestered in the extracellular matrix of cementum, and we have purified and characterized one of the growth factors. This growth factor, the cementum derived growth factor (CGF), was purified from bovine cementum by acetic acid extraction followed by heparin affinity chromatography and HPLC using cation exchange, molecular sieve, and reverse-phase columns. NaDodSO4-polyacrylamide gel electrophoresis of purified CGF preparation revealed the presence of two major protein bands migrating with Mr 18,000-22,000 and 14,000-16,000. The latter was associated with the major part of the mitogenic activity. The activity of CGF was inhibited by antibodies to insulin-like growth factor-I (IGF-I) and IGF-I receptor. Both CGF and IGF-I were mitogenic to human gingival fibroblasts and alveolar bone cells, but the bone cells responded better to CGF than to IGF-I. The IGF-I did not bind to heparin-sepharose, while CGF bound to it and was eluted with 0.6M NaCl from heparin-sepharose columns. Heparin-sepharose 0.2M NaCl fractions of cementum extracts contained IGF-I migrating with Mr 7,500, but its mobility was not affected by N-glycosidase treatment. Western analysis using anti-IGF-I antibodies showed that CGF preparations contained cross-reacting species migrating with Mr 18,000-22,000, 14,000-16,000 and 11,000-12,000, however after treatment with N-glycosidase the Mr 18,000-22,000 component was absent. Internal amino acid sequences of six tryptic peptides of CGF were determined by microsequencing. The sequence of one 15-amino acid long peptide was the same as the receptor binding domain of IGF-I, and another 9-amino acid peptide had 78 % homology to a sequence derived from an untranslated region of sheep IGF-I exon 1. Four other peptides had no apparent homology with IGF-I. From these results we conclude that the CGF is an IGF-I like molecule.

Amino Acid Sequence↗
PubMed2006

Dilacerated unerupted central incisor: A case report.

Dilaceration of permanent successors is one of the most common complications of trauma to the deciduous teeth. It is advisable to keep these patients under observation post-trauma and to consult an orthodontist at an early stage to prevent unfavorable sequelae. Presented here is a typical case of dilaceration.

Child↗
PubMedDate not supplied

Multiple dental developmental abnormalities following canine distemper infection.

Multiple dental developmental abnormalities (e.g., dental impaction, partial eruption, oligodontia, enamel hypoplasia, and dentin hypoplasia) in a 10-month-old, female, Tanzanian tropical mixed-breed puppy are reported. Various permanent teeth were involved. These included impacted mandibular canine and first and third premolar teeth; a partially erupted maxillary canine tooth; oligodontia of a mandibular fourth premolar tooth; enamel hypoplasia of the maxillary and mandibular canine teeth, incisors, and premolars; and dentin hypoplasia of the maxillary incisors, maxillary premolars, and mandibular premolars. The puppy had clinical canine distemper at the age of two months and had no history of any other systemic nor generalized infection prior to the time when the dental abnormalities were observed.

Animals↗
PubMed1998

Furcation involvements: therapeutic considerations.

The successful treatment of furcation-involved multirooted teeth has been a challenge for many clinicians. Furcation lesions have been treated by osseous resection, root amputation, tooth hemisection, and other methods. Recently, guided tissue regeneration has been considered the treatment of choice, but inconsistent treatment outcomes have caused frustration for many clinicians. This article discusses the complexity of the furcation lesion, important aspects of the lesion and its relation to the tooth anatomy, and treatment options that should be considered.

Dental Implants↗
PubMed1997

Panoramic radiographic examination of edentulous jaws.

Many dentists routinely make panoramic radiographs of edentulous patients who request replacement dentures. A study was conducted in patients who attended a Jordanian dental school seeking a new set of dentures. Patients who had at least one set of complete dentures and were treated elsewhere, were included in this study. Any patient whose clinical findings suggested a need for further radiographic study was excluded. Of 286 patients (572 edentulous arches), only three were found to have impacted molars that required extraction and altered the treatment plan. The use of radiography in dentistry generates a large expense and there are risks of radiation hazards. The routine use of radiographs for patients who wear complete dentures and need replacement dentures should be discouraged. A thorough history must be obtained and a careful clinical examination must be performed for the diagnosis and treatment planning.

Aged↗
PubMed1975

The transplantation and surgical movement of developing teeth.

There has now been one hundred years of study, research and clinical application into the possibility of transplantation of tooth germs, and a short review of the literature is given. Two clinical cases are described in which replacement of upper incisors has been successfully achieved by auto transplantation of a developing lower bicuspid and upper canines respectively. Long term follow up of such cases now extends over a decade and it is suggested that this technique has reached a stage now hen it can be recommended to the dental profession generally as a worthwhile clinical approach.

Accidents↗
PubMed1999

[Management of an impacted canine tooth in the palate].

The abundance of scientific articles about current dental movements such as intrusion, space closure, uprighting ... is in contrast with the rarity of those concerning palatal impacted canine. The goal of this article is to emphasize upon frequent hazards of this dysmorphosis which often generate litigations and to suggest an original classification of favorable and unfavorable clinical situations and a simplified mechanotherapy.

Cephalometry↗
PubMedDate not supplied

Fracture resistance of endodontically treated teeth restored with bonded amalgam and full crowns.

The use of dentin bonding prior to placement of core build-up restorations has been shown to reduce microleakage and reinforce remaining tooth structure. However, information is lacking about the influence a core build-up has on the fracture resistance of crowned teeth. The purpose of this in-vitro study was to compare the influence of three types of core build-ups on the fracture resistance of crowned teeth.

Analysis of Variance↗
PubMedDate not supplied

Tissue preservation and maintenance of optimum esthetics: a clinical report.

Today, most tooth replacement in the esthetic zone is done using implants placed in a delayed surgical protocol. Unfortunately, this delay can result in loss of both hard and soft tissue during the healing period, necessitating guided tissue regeneration techniques at the time of implant placement. Recent developments with tapered implants have facilitated predictable immediate implant placement, preserving the osseous structure surrounding the socket. Further developments with custom healing abutments can preserve the crestal soft tissues, including the papillae. This article reviews techniques that provide for the preservation of both bone and soft tissue while enhancing the esthetic results around implants.

Bicuspid↗