PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “VASOTOCIN”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 883 records · Page 49Linked to original sources
PubMed1999

Preoptic GnRH and AVT: axes for sexual plasticity in teleost fish.

Alternative reproductive tactics within one sex, adult sex or role change, and reproductive suppression are all forms of reproductive plasticity commonly exhibited among teleost fishes. The two neuropeptides that have been most extensively studied with regard to such behavioral plasticity are gonadotropin releasing hormone (GnRH) and arginine vasotocin (AVT). Here, we review intra- and intersexual variation in the number and size of GnRH and AVT neurons along with gonadal phenotype in those species of teleosts showing intraspecific plasticity in reproductive behavior. In several species, male dimorphisms in the number and/or size of GnRH neurons in the forebrain's preoptic area parallel a divergence in relative gonad size and reproductive tactics. The available studies of AVT-containing neurons in the preoptic area also indicate intrasexual dimorphisms among males, although a proximate link to other reproductive traits and behavioral outcomes is more difficult to recognize. For both GnRH and AVT, there are also species-typical patterns in the coupling between structural (e.g., neuronal and gonadal) traits and reproductive tactic expressed, which likely reflect distinct patterns of adaptation to particular ecological environments. As discussed, neurophysiological, biochemical, and receptor density studies are now essential to establish the functional significance of the diverse organizational patterns of GnRH and AVT neurons in teleosts. Similar studies also need to be carried out in species of other vertebrate groups that show comparable behavioral plasticity.

Animals↗
PubMed1998

Neurally active stress peptide inhibits territorial defense in wild birds.

It is unclear whether the behavioral effects of peptides in laboratory studies always reflect natural conditions. Here we test whether we can detect measurable behavioral changes after rapidly injecting peptides into the brains of wild birds. We used a modified stereotaxic-like technique to inject corticotrophin-releasing factor (CRF) and arginine vasotocin (AVT, the nonmammalian form of arginine vasopressin), two hormones important in the stress response, into the brains of wild, freely behaving, male white-crowned sparrows (Zonotrichia leucophrys). We then monitored subsequent territorial behavior to determine whether CRF or AVT altered this behavior. Surprisingly, the potent stressors of capture and surgery did not eliminate territorial behavior, with many birds resuming territorial defense within 60-90 min after surgery. Centrally acting CRF, however, significantly reduced territorial defense whereas centrally acting AVT had no effect. These results indicate that the behavioral affects of peptides can be studied under natural conditions.

Animals↗
PubMed2001

Manipulations of the AVT system shift social status and related courtship and aggressive behavior in the bluehead wrasse.

Arginine vasotocin (AVT) and its mammalian homologoue arginine vasopressin (AVP) influence male sexual and aggressive behaviors in many species. We tested the effects of AVT and an AVP-V(1a) receptor antagonist on the display of alternative male tactics in a tropical coral reef fish, the bluehead wrasse Thalassoma bifasciatum. We gave AVT injections to territorial and nonterritorial males of the large and colorful phenotype (terminal phase) and an AVP-V(1a) receptor antagonist, Manning compound, to territorial males in the field. AVT increased courtship independent of status, while its effects on territoriality and aggression were dependent upon male status. In territorial males, AVT increased courtship and tended to decrease the number of chases toward initial phase individuals. In nonterritorial males, AVT increased courtship, chases toward initial phase individuals, and territorial behavior while decreasing feeding. These are all behaviors rarely seen in nonterritorial males, so AVT made these males act like territorial TP males. The AVP-V(1a) receptor antagonist had opposite effects. It decreased courtship and territorial defense, making these males act more like nonterritorial males. Manipulations of the AVT system shifted males within a single phenotype from the nonterritorial social status to the territorial social status and vice versa. Since the entire suite of behaviors related to territoriality was affected by AVT system manipulations, our results suggest that the AVT system may play a key role in motivation of behaviors related to mating.

Aggression↗
PubMed1992

Hormone-induced vocal behavior and midbrain auditory sensitivity in the green treefrog, Hyla cinerea.

Twenty four castrated male, 6 intact male, and 11 intact female Hyla cinerea were injected subcutaneously with 25 micrograms arginine-vasotocin (AVT) and induced to call 1 h later in response to the playback of a conspecific mating call. Eighteen castrated males and 8 intact females were implanted 5 mg androgen pellets for 3 weeks prior to the neuropeptide injection. Among castrated males, 6/9 testosterone (T) implanted, 4/9 dihydrotestosterone (DHT) implanted and 2/6 non implanted individuals produced calls after being administered AVT. 5/6 intact non implanted males and 6/8 T intact implanted females also called, and 3 intact non implanted females remained silent after the injection. Evoked calls had a mid-frequency spectral peak at about 1900 Hz which is absent in field-recorded mating calls of this species. Calls of implanted females and castrated non implanted males were shorter than those of castrated implanted and intact non implanted males. Audiograms measured before hormone implants showed dips of enhanced sensitivity at about 0.5, 0.9 and 3.0 kHz in males and females. After AVT injection, thresholds at frequencies within the 0.7-1.5 kHz range were increased in castrated males. Such reduction in sensitivity points to an inhibition of the auditory system during hormone induced vocal activation.

Acoustic Stimulation↗
PubMed1978

Immunocytochemical study of the neurohypophysial hormone producing system of the lungfish, Protopterus aethiopicus.

Using the unlabeled antibody peroxidase-antiperoxidase complex (PAP) technique at the light microscopic level, it has been shown that, in the dipnoan preoptico-hypophysial neurosecretory system, vasotocin and mesotocin are synthesized in separate neurons. In the preoptic nuclei, the perikarya of these two types of neurosecretory neurons are not located preferentially. The two types of neurosecretory perikarya give rise to separate vasotocinergic and mesotocinergic axons, respectively. The dipnoan median eminence and neural lobe contain separate vasotocinergic and mesotocinergic nerve fibres, the general distribution of which is described. In the pars distalis and the pars intermedia of the hypophysis, neurohypophysial hormone-containing nerve fibres have not been found.

Animals↗
PubMed1987

Immunocytochemical and ultrastructural evidence for a neurophysinergic innervation of the subcommissural organ of the snake Natrix maura.

The subcommissural organ (SCO) of the snake Natrix maura was studied by use of the immunoperoxidase procedure. Primary antisera against bovine neurophysins (Nps I + II, OXY-Np), oxytocin (OXY), mesotocin (MST), arginine-vasotocin (AVT), somatostatin (SOM), beta-endorphin (END) and bovine Reissner's fiber were used. A conventional ultrastructural study, with special emphasis on the nerve fibers present in the SCO, was also performed. Nerve fibers containing immunoreactive OXY-Np and MST were seen to reach the SCO. The staining of adjacent sections with the anti-Reissner's fiber serum showed that the OXY-Np- and MST-immunoreactive fibers were distributed among the cell bodies and processes of the ependymal secretory cells. No fibers containing immunoreactive OXY, AVT, SOM or END were found in the SCO. The ultrastructural analysis revealed in the SCO the presence of nerve fibers filled with electron-dense granules, 170-210 nm in diameter. Although a direct apposition between these fibers and the SCO cells was frequently seen, no synaptic differentiations were identified. Structures identical to the Herring bodies (found in the neurohypophysis) were seen in the SCO.

Animals↗
PubMed1976

Immunocytochemical demonstration of separate vasotocinergic and mesotocinergic neurons in the amphibian hypothalamic magnocellular neurosecretory system.

Using the unlabeled antibody peroxidase-antiperoxidase (PAP) technique at the light microscopic level, it was demonstrated that, in the amphibian magnocellular hypothalamo-hypophysial neurosecretory system, vasotocin and mesotocin are synthesized in separate neurons. A tendency to preferential location of the two kinds of neuronal perikarya is described. The neurosecretory perikarya are the origin of separate vasotocinergic and mesotocinergic axons. In the neural lobe, the pattern of distribution of the two types of axons is different. The coarse ventricular "dendrites" of both kinds of neurons are hormone-containing processes. Staining with anti-bovine neurophysin I serum suggested that the vasotocinergic and the mesotocinergic neurons synthesize different neurophysins.

Animals↗
PubMed1977

Immunocytochemical investigation of the hypothalamo-neurohypophysial system in birds.

The results of an immunohistochemical investigation of the hypothalamo-neurohypophysial system in several species of birds have shown that: (1) mesotocin and vasotocin are synthesized in separate neurons; (2) in all species investigated the distribution of mesotocinergic and vasotocinergic perikarya follows a common pattern; (3) the external zone of the avian anterior median eminence contains exclusively vasotocinergic nerve fibers, originating in supraoptic and ventral paraventricular regions; (4) the distribution of immunoreactive elements in the neural lobe shows a definite species-dependent pattern.

Animals↗
PubMed1986

Immunocytochemical study of the development of vasotocin/mesotocin in the hypothalamo-hypophysial system of the chick embryo.

The hypothalamo-hypophysial system of the chick embryo has been studied with a monoclonal antibody which cross-reacts with arginine vasotocin and mesotocin, using thick (100 micron) sections in conjunction with a peroxidase-conjugated rabbit anti-mouse antibody. Although weakly stained perikarya occur occasionally in the tuberal region on embryonic days 6 and 7, the most consistent immunostaining of perikarya is found in the periventricular region of the caudal midhypothalamus at the level of the optic chiasm after embryonic day 8 1/2. Synthesis of peptides, therefore, takes place while the cells are close to their site of origin. Between embryonic days 9 and 10, beaded axons run along the anterior median eminence closely apposed to the adenohypophysis, thereby forming the anlage of the zona externa. The axons of the hypothalamo-neurohypophysial tract surround the neural lobe between embryonic days 11 and 12. The caudal to rostral wave of neuronal maturation that occurs during development appears to be due to a progressive differentiation of the periventricular zone, as well as the migration of perikarya. The early periventricular perikarya at embryonic day 8 1/2 send processes rostrally in a wing-shaped formation that extends both dorso- and ventrolaterally. From embryonic days 10 to 12, perikarya can be observed in the wing-like extensions, apparently migrating to rostral levels. The dorsolateral pathway gives rise at its midportion to the lateral cell group, whereas those perikarya migrating more laterally form the anlage of the external supraoptic nucleus. The ventrolateral wing-shaped extension of perikarya appears to be directed toward the ventral group and those lateral perikarya continuous with it. The location of mature neuronal cell groups is well established by embryonic day 17.

Animals↗
PubMed1991

Changes in expression of provasotocin and proisotocin genes during adaptation to hyper- and hypo-osmotic environments in rainbow trout.

The physiological roles of neurohypophysial hormones, vasotocin (VT) and isotocin (IT), are not yet clear in teleosts. Since information on responsiveness of hypothalamic neurosecretory neurons to environmental stimuli may contribute to an understanding of their physiological roles, effects of environmental hyper- and hypo-osmotic stimuli on expressions of VT and IT precursor (proVT and proIT) genes in rainbow trout were investigated, using an in situ hybridization technique in which 46 mer synthetic oligonucleotides were used as hybridization probes. The probes corresponded to the mRNA loci encoding chum salmon proVT (-5 to 11) and proIT (-5 to 11), and were labeled at the 3'-end with 35S. Autoradiographic silver grains which represent the hybridization signals of proVT and proIT mRNAs were localized in both magnocellular and parvocellular neurons in the nucleus preopticus magnocellularis (NPOmg). Localizations of proVT and proIT hybridization signals coincided with those of VT- and IT-immunoreactive neurons in adjacent sections, and showed that proVT and proIT genes are expressed in separate neurons. The intensity of proVT hybridization signals as determined by grain counting in magnocellular neurons in the NPOmg was conspicuously decreased after transfer from fresh water (FW) to 80% seawater (SW). The proVT mRNA levels in SW trout were consistently lower than those of FW trout for up to 2 weeks. After return from 80% SW to FW, the proVT mRNA level increased, attaining the initial FW level. The proIT mRNA levels in SW trout were not statistically different from those in FW trout, except for the 1st day after transfer to SW.(ABSTRACT TRUNCATED AT 250 WORDS)

Amino Acid Sequence↗
PubMed1990

Arterial hypotension in ducks adapted to high salt intake.

A homogeneous group of 8-week-old Pekin ducks was divided into two groups: saltwater (SW) ducks received salt water of gradually increasing salinity (200-600 mOsm.kg-1) from the 8th to 20th week of age; freshwater (FW) ducks were maintained on fresh water but otherwise treated identically. During the course of salt-adaptation SW ducks increased plasma osmolality, Na+ and Cl- levels, and concentrations of the osmoregulatory peptide hormones arginine vasotocin and angiotensin II. The apparent volume of inulin distribution decreased in SW ducks, but blood volume was not reduced. SW ducks also developed arterial hypotension, bradycardia, and reduced cardiac output in the course of salt adaptation. This depressed cardiovascular performance was associated with enhanced vagal restraint of cardiac function and reduced plasma concentrations of norepinephrine. Salt water adaptation did not alter the degrees to which mean arterial pressure and heart rate changed in response to intravenous bolus injections of catecholamines. The same applied to the osmoregulatory peptides which were, however, effective only at supraphysiological concentrations. The Pekin duck, as a bird predisposed for adaptation to high salt loads, presumably adapts to chronic hypertonic saline intake by resetting the central autonomic control of blood pressure to a lower level.

Adaptation, Physiological↗
PubMed1980

Immunocytochemical evidence for the presence of an albumin-like substance in the rat pineal gland.

Two rabbits and two guinea pigs were immunized with arginine vasotocin (AVT) conjugated to bovine albumin with glutaraldehyde. Only one preparation of antiserum (anti-G 7) was of value. Anti-G 7 immunochemically defined the same rat pineal cells previously reported as presumptive AVT cells. However, absorption of anti-G 7 with bovine albumin inhibited the staining of the pineal cells demonstrating that they contained an albumin-like substance. Positive immunochemical staining of the rat pars nervosa suggested that anti-G 7 contained antibodies able to react with arginine vasopressin (AVP). Loss of a positive reaction in the posterior lobe on absorption of anti-G 7 with AVT or AVP confirmed this. However, the addition of AVT to anti-G 7 failed to inhibit the immunochemical staining of the pineal cells. This study reports the presence of an albumin-like substance in pineal cells previously described as presumptive AVT cells, and discusses possible explanations for the inability of anti-G 7 to recognize immunocytochemically the native AVT molecule. Confirmation of AVT in the pineal gland by immunocytochemistry must await the availability of more specific antisera.

Albumins↗
PubMed1988

Electrophysiological analysis of sodium-transport in the colon of the frog (Rana esculenta). Modulation of apical membrane properties by antidiuretic hormone.

Sodium transport and apical bioelectrical membrane properties were investigated in frog colonic epithelium in the absence and presence of the antidiuretic hormone arginine-vasotocin (AVT). Apical Na-permeability and intracellular Na-activity were evaluated by analysis of current-voltage relationships in the serosally K-depolarized tissue. Tissue- and apical membrane capacitance were measured by voltages step analysis. The frog colon was found to be a tight epithelium with a transepithelial resistance of 2.63 +/- 0.25 k omega.muF (n = 17). 85-90% of short circuit current (11.2 +/- 1.1 microA.microF.l-1; n = 17) was related to electrogenic Na-transport from mucosa to serosa. Graded doses of amiloride (less than 50 mumol.l-1) induced Michaelis-Menten-type inhibition kinetics. Serosal addition of 10(-6) mol.l-1 AVT induced a significant increase in sodium current (25%), apical sodium permeability (19%) and tissue capacitance (4.3%) whereas intracellular Na-activity remained unchanged. There was a good correlation between increased Na-current and apical Na-permeability. No correlation was found between Na-current and membrane capacitance. Our results demonstrate that in contrast to other species the amphibian colon shows a natriferic reaction to AVT. We suggest that the regulation of Na-transport in frog colon is similar to that in the toad urinary bladder. It is caused by an activation of preexisting apical Na-channels and not by fusion of subapical cytoplasmic vesicles with the apical membrane.

Amiloride↗
PubMed1986

Thermally induced changes in neural and hormonal control of osmoregulation in a bird with salt glands (Anas platyrhynchos).

In conscious Pekin ducks adapted to hypertonic saline (1.9%) as drinking water, steady state secretion of the salt glands was established by continuous intravenous salt loading and the effects of hypothalamic thermal stimulation on salt gland activity and on the plasma concentrations of arginine vasotocin (AVT) and angiotensin II (AII) were observed. Hypothalamic cooling depressed salt gland secretion and the plasma level of AVT. Hypothalamic warming caused transient activation and subsequent inhibition of salt gland secretion without consistent changes of the plasma levels of AVT and AII. Whole body cooling by heat extraction with a colonic thermode produced moderate inhibition of salt gland activity, without changes in plasma AVT and AII, which may be explained by peripheral vasoconstriction. The results are consistent with the view that hypothalamic osmoregulation is under an influence of local temperature by combined osmo/thermo-responsiveness of hypothalamic neurons and temperature dependence of signal transmission in hypothalamic neural integration of osmoregulation.

Angiotensin II↗
PubMed1988

Contributions of the kidneys and intestines to water conservation, and plasma levels of antidiuretic hormone, during dehydration in house sparrows (Passer domesticus).

The contributions of the kidneys, the small intestine and the lower intestine (rectum plus cloaca) to water conservation during dehydration in unanaesthetized, unrestrained house sparrows (Passer domesticus) were assessed. Thirty hours of acute dehydration resulted in a 12% loss in body mass and a significant increase in plasma osmolality. Glomerular filtration rate declined by 55%, from 7.7 to 3.5 ml/h, and urine flow rate delined by more than 80%, from 0.2 to 0.03 ml/h. These changes are likely attributable to a large increase in plasma levels of arginine vasotocin during dehydration, from less than 26 pg/ml in hydrated birds to greater than 200 pg/ml after 30 h dehydration. Flow of water from the ileum to the lower intestine was reduced during dehydration, primarily because of a reduced flow of dry matter (with no significant reduction in water content). The rate of water loss in the excreta declined from 0.2 ml/h in hydrated birds to 0.04 ml/h in dehydrated birds. The rate of water reabsorption in the lower intestine (equal to the rate of water loss in the excreta minus the combined rates of inflow into the lower intestine from the urine and the ileal contents) slightly exceeded the rate of water flow from the ileum in both hydrated and dehydrated birds. We suggest that much of the water reabsorbed in the lower intestine of hydrated birds derives from the urine, but that primarily water from ileal contents is reabsorbed in dehydrated birds. That is, urine undergoes significant post-renal modification in hydrated but not dehydrated house sparrows.

Animals↗
PubMed1984

Specificity of a cytochemical bioassay for arginine-vasopressin and its validation for plasma measurement.

The total Na+/K+ ATP-ase activity of the thick ascending limb of the loop of Henle may be stimulated by arginine-vasopressin (AVP). Lysine-vasopressin (LVP), oxytocin (OT), and arginine-vasotocin (AVT) produce less than 5% of the enzyme activity induced by the same concentration of AVP. Physiological concentrations of a mixture of other hormones with known activity on the kidney (T3, T4, aldosterone, angiotensin II, and OT) did not significantly increase total Na+/K+ ATP-ase activity. Specific AVP antiserum consistently removed greater than 90% of the stimulatory effect of plasma. The concentration of AVP in plasmas from dehydrated subjects was greater than 10 times that of the same subjects hydrated. Intra-assay coefficient of variation was 35% and 52% from 200 microliters and 20 microliters of plasma respectively. The interassay coefficient of variation was 53% and 55% from plasma pools with high and low AVP content.

Animals↗
PubMed1983

Inhibition by naloxone of the serotonin-induced prolactin release in free-moving rats.

The effect of the opiate antagonist naloxone on serum prolactin after treatment with serotonin, arginine vasotocin (AVT) or melatonin was studied in prepubertal and adult unanesthetized rats. Prolactin was quantified in blood samples withdrawn through an intrajugular silastic cannula from undisturbed ovariectomized adult rats. After taking a basal sample, animals were injected through the cannula with naloxone (0.8 mg/kg) and 5 min later with serotonin creatine sulphate (6.4 mg/kg), AVT (20 micrograms/kg), melatonin (4 mg/kg) or saline; new samples were taken 15 and 30 min thereafter. Injection of serotonin was followed by a 10-fold increase of prolactin levels 15 min later; this increase was drastically reduced, although not abolished, by pretreatment with naloxone. In animals injected with saline, AVT or melatonin, no significant changes in serum prolactin were observed. In a second group of experiments, 30 day-old female rats injected with serotonin creatinine sulphate (10 mg/kg, i.p.) exhibited a 6-fold increase in serum prolactin 15 min after injection; this increment was reduced but not abolished by pretreatment with naloxone (5 mg/kg, i.p.). It is postulated that the prolactin releasing effect of serotonin is mediated, at least in part, by an opioid receptor.

Animals↗