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Results for “Decalcification Technique”

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At least 91 records · Page 5Linked to original sources
PubMed1991

[Frame-mounted anastomosis of veins using a microsurgical technique and demineralized tubular allobone].

The experimental-morphological analysis covers the results of 62 operations for establishment of major vein anastomosis. In three series of experiments microsurgical suturing was used, while the control series employed the techniques of general surgery. In the microsurgery group, the best outcome (100 percent patency without anticoagulant administration) was achieved in cases when the venous anastomosis was protected by the demineralized tubular bone. Good outcomes were seen in cases with application of the original technique of mounting the venous anastomosis on rings of tubular bone. The advantages of microsurgical frame-mounted suturing of major veins over the general surgical techniques are demonstrated.

Anastomosis, Surgical↗
PubMed2003

Decalcifying effect of EDTA-T, 10% citric acid, and 17% EDTA on root canal dentin.

OBJECTIVE: An in vitro study was conducted to determine the efficacy of 17% EDTA plus 1.25% sodium lauryl ether sulfate (EDTA-T), 10% citric acid, and 17% EDTA with respect to Ca(++) extraction. STUDY DESIGN: The root canals of 90 extracted human canines were instrumented by using the step-back technique. The teeth were randomly divided into 9 groups of 10 teeth each according to the solution and time intervals involved (ie, 3, 10, and 15 minutes). The Ca(++) concentration of the solution was determined by using atomic absorption spectroscopy. RESULTS: At 3 minutes, there was no significant difference between Ca(++) extraction by 10% citric acid and 17% EDTA (P >.05). EDTA-T had the least efficacy for Ca(++) extraction (P <.05). At 10 minutes and 15 minutes, there were no significant differences between 10% citric acid and 17% EDTA or between EDTA-T and 17% EDTA (P >.05). However, 10% citric acid was significantly better than EDTA-T in terms of Ca(++) extraction (P <.05). CONCLUSION: Both 10% citric acid and 17% EDTA are good decalcifying agents when the step-back technique is used.

Chelating Agents↗
PubMed2005

An in vitro model to investigate filling of lateral canals.

Aims of this work were to examine lateral canals in extracted teeth, to propose a new technique to produce artificial lateral canals, and to compare two obturation techniques. Cleared roots were examined to record measure and shape of lateral canals. Artificial lateral canals were prepared on human demineralized teeth before final clearing. Specimens were divided in two groups: canals of group 1 were filled with Schilder's technique, canals of group 2 were filled with vertical compaction with apical backfilling. Stereomicroscopic analysis of lateral canal filling revealed lower filling rates in apical canals compared to coronal ones and higher filling rates with "vertical compaction with apical backfilling" compared to Schilder's group. The tested procedure appears to be a reliable technique to obtain standardized lateral canals and to compare filling procedures.

Decalcification Technique↗
PubMed1988

Computer-assisted densitometric image analysis in periodontal radiography. A methodological study.

A videobased computer assisted densitometric image analysis (CADIA) system to quantify alveolar bone density changes on standardized dental radiographs was tested. An algorithm was used for grey level correction of a subsequent image to the baseline image. Quantitative information regarding positive and/or negative grey level changes were obtained automatically. Comparison of the ability of CADIA to detect surgically induced bone loss with interpretation of digital subtraction images and conventional radiographic interpretation revealed that CADIA was the most sensitive of the 3 methods, followed by interpretation of digital subtraction images which was considerably more sensitive than conventional radiographic interpretation. CADIA was capable of assessing differences in alveolar bone changes due to periodontal surgery between sites exposed to ostectomy/osteoplasty and control sites and sites exposed to periodontal surgery without ostectomy/osteoplasty. Finally, CADIA was capable of assessing differences in remodeling activity over 4-6 weeks after periodontal surgery between 45 surgical sites and 45 control sites. The system offers an objective method to quantitatively follow alveolar bone density changes over time and appears to be the most sensitive of previously described radiographic interpretation techniques.

Absorptiometry, Photon↗
PubMed1981

Preparation of small temporal bones for high-resolution light microscopy.

A technique was used for rapid processing of small temporal bones for high-resolution light microscopy, Temporal bones were decalcified in edetic acid and embedded in glycol methacrylate. Sections (thickness, 1 micrometer) were stained with a variety of stains that demonstrated cellular details not usually seen with conventional methods. This technique is suitable for routine histologic, histochemical, and radioautographic studies of small temporal bones.

Animals↗
PubMed1988

A new decalcifying technique for immunohistochemical studies of calcified tissue, especially applicable to cell surface marker demonstration.

We have developed a new decalcifying technique for use in light and electron microscopy studies utilizing immunohistochemical staining of calcified tissues. Specimens containing calcified tissues can be adequately decalcified at a pH of 7.1-7.4 and temperature of -5 degrees C, without freezing, by use of a solution containing EDTA, sodium hydroxide, and glycerol. In this study, Leu-2a, Leu-3a, Leu-4, Leu-7, Leu-12, Leu-14, Leu-M1, Leu-M2, Leu-M3, and HLA-DR-positive cells in destructive lesions of bone tissues from patients with rheumatoid arthritis and osteomyelitis were successfully detected immunohistochemically. Furthermore, the presence of HLA-DR antigen on the surface of the infiltrating cells in the same lesions could be demonstrated using the immunoelectron microscopic technique. The results reported here have not previously been obtainable using conventional decalcifying techniques.

Antibodies, Monoclonal↗
PubMed1992

The microwave oven in temporal bone research.

The microwave oven has recently been introduced in morphological research. It can be used for virtually all procedures in histological processing; fixation, dehydration, embedding, staining, immunotechniques, and the techniques used for electron microscopy. Here we report that the microwave oven histoprocedures also have several advantages in temporal bone research on animals. The microwave oven has been tested for (i) fixation, (ii) decalcification, (ii) staining of semithin plastic sections. The microwave oven procedures are time-saving and results in an excellent preservation of the tissues. The microwave histoprocessing should also be applicable to temporal bone research in human.

Animals↗
PubMed1986

Healing responses to grafting of combined collagen-decalcified bone in periodontal defects in dogs.

The feasibility of a new combined collagen gel-autolysed antigen-extracted allogeneic bone implant was tested. Its effect on gaining new attachment in surgically created defects in four dogs was evaluated over 24 weeks. As controls, sites receiving implants of the bone implant alone, nonimplanted and untreated defects were also evaluated. The collagen gel encouraged ingrowth of regenerative tissue-fibroblasts in the early stages of wound healing while the allogeneic bone induced new bone formation. The graft materials were bicompatible, technically manageable and clinically effective. From the present preliminary study, it was evident that the combined autolysed antigen-extracted allogeneic bone-collagen gel implant may offer advantages over the present implant materials and techniques currently used in the treatment of periodontal intrabony defects.

Alveolar Process↗