PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “ISOFLUROPHATE”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 91 records · Page 5Linked to original sources
PubMed1963

COMPLEMENT AS A MEDIATOR OF INFLAMMATION. ENHANCEMENT OF VASCULAR PERMEABILITY BY PURIFIED HUMAN C'1 ESTERASE.

Purified preparations of the esterase derived from the first component of complement (C'1 esterase) increased vascular permeability in guinea pig skin, an effect inhibited by triprolidine, an antihistaminic agent, but not by soy bean trypsin inhibitor. The permeability-increasing and esterolytic properties of C'1 esterase were inhibited in parallel by the serum inhibitor of C'1 esterase, diisopropylphosphofluoridate and extremes of temperature and pH. Moreover, the permeability-increasing and esterolytic properties evolved in parallel when C'1 esterase was generated from its subcomponents. How C'1 esterase induces changes in vascular permeability remains unexplained, although the possibility that its action is mediated through a histamine-like agent is attractive.

Animals↗
PubMed1965

THE LYSIS OF CELL WALLS OF GROUP A STREPTOCOCCI BY STREPTOMYCES ALBUS ENZYME TREATED WITH DIISOPROPYL FLUOROPHOSPHATE. CHARACTERISTICS OF THE LYTIC REACTION AND THE SOLUBLE CELL WALL FRAGMENTS.

Diisopropyl fluorophosphate (DFP) effectively inhibited proteolytic activity in preparations of partially purified Streptomyces albus enzyme used to lyse cell walls of Group A streptococci. Lysis of non-trypsinized Group A cell walls with DFP-treated S. albus enzyme released a soluble protein fraction containing antigenic type-specific M protein, a carbohydrate fraction consisting of Group A and a small amount of A-variant polysaccharides, and a dialyzable fraction. The similarities of the products of DFP-treated S. albus enzyme lysis of streptococcal cell walls to those released by phage muralytic enzyme furnish additional evidence of the close relationship of these wall lysins. In view of small differences in electrophoretic mobility, immunodiffusion, and chemical composition, it is suggested that Group A streptococcal cell wall polysaccharide dissolved by DFP-S. albus enzyme consists of a spectrum of molecules having the same immunological determinants but differing in content of conjugated mucopeptide.

Bacteriolysis↗
PubMed1965

C'1 ESTERASE EFFECT ON ACTIVITY AND PHYSICOCHEMICAL PROPERTIES OF THE FOURTH COMPONENT OF COMPLEMENT.

Highly purified C'1 esterase of human serum is capable of inactivating isolated fourth component of human complement (beta(1E)-globulin). Inactivation is accompanied by changes in electrophoretic and ultracentrifugal properties of beta(1E)-globulin. If non-sensitized sheep erythrocytes are present during the action of C'1 esterase on beta(1E)-globulin, a complex is formed consisting of cells and cytolytically active fourth component (EC'4). Thus, inactivation of beta(1E)-globulin by C'1 esterase appears to be preceded by a state of activation enabling beta(1E)-molecules to combine with cell membrane receptors. Acceptor groups appear to be present also in 7S gamma-globulin and in beta(1E)-globulin itself, since C'1 esterase can induce the formation of beta-beta and of beta(1E)-7S gamma-globulin complexes.

Animals↗
PubMed1960

The use of DFP32 as a red cell tag with and without simultaneous tagging with chromium 51 in certain animals in the presence or absence of random destruction.

DFP(32), used to label erythrocytes in vitro, combines with cell constituents in two stages, the first almost immediate and involving tributyrinase inactivation, the second slower (more than 40 minutes) involving cholinesterase inactivation. Raising the DFP concentration increases the amount irreversibly bound, but increases even more the immediate post-transfusion elution, and DFP is unsuited for investigating erythrocyte viability of stored samples. In vivo tagging by intramuscular injection is satisfactory and normal survival curves are linear since the sample tagged has normal age distribution of cells in absence of random destruction. Here DFP(32) curves are easier to interpret than Cr(51) curves. In sheep, chromium elution occurs at two different rates producing a rapid initial drop followed by a slower one of about 3 per cent daily. Random destruction alters cell age distribution. New equations are derived for cases in which this is constant both with and without chromium elution; they were applied satisfactorily to dog and sheep blood. Analysis of such curves is difficult; approximate values for random destruction rates can be obtained though not potential life spans. Chromium curves can be analyzed only with the help of DFP(32) or similar curves, and yield little additional information. DFP(32) and chromium can be used simultaneously to provide controls.

Animals↗