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PubMed1996

Effect of bifidogenic factors on growth characteristics of bifidobacteria in infant formulas.

A bifidogenic factor, lactulose or fructooligosaccharides, was added (0.5%) into infant formula based on soy or infant formula with hydrolyzed casein. The infant formulas were then inoculated (2.5%) with Bifidobacterium bifidum (ATCC 15696), Bifidobacterium breve (ATCC 15700), Bifidobacterium infantis (ATCC 15697), or Bifidobacterium longum (ATCC 15708) or their mixture (mixed culture) and incubated at 37 degrees C for 24 h. Lactulose did not influence maximal counts or generation times in either formula for any species except B. infantis, which had lower counts. Trends of developed acidity and pH of the mixed culture in the infant formulas with or without lactulose were similar to those for B. breve. Maximal counts and generation times remained unchanged with or without fructooligosaccharides for all species and the mixed culture, except for B. bifidum in the formula based on soy, for which maximal counts did not occur. Growth in either formula was inhibited for B. infantis with lactulose and B. breve with fructooligosaccharides past 8 h of inoculation.

Acetic Acid↗

PubMed2005

Survival of Enterobacter sakazakii in a dehydrated powdered infant formula.

A quantity of dehydrated powdered infant formula was prepared to contain Enterobacter sakazakii strain 607 at approximately 106 CFU/ml when rehydrated according to the manufacturer's instructions. The survival of the microorganism in the dry formula was followed for 2 years, during which samples periodically were rehydrated and analyzed for viable E. sakazakii. During the initial 5 months of storage at room temperature, viable counts declined approximately 2.4 log cycles. During the subsequent 19 months, the concentration of viable E. sakazakii declined an additional 1.0 log cycle. These results indicate that a small percentage of E. sakazakii cells can survive for extended periods in dehydrated powdered infant formula.

Colony Count, Microbial↗

PubMed1996

Influence of the derivatization procedure on the results of the gaschromatographic fatty acid analysis of human milk and infant formulae.

Many different analytical procedures for fatty acid analysis of infant formulae and human milk are described. The objective was to study possible pitfalls in the use of different acid-catalyzed procedures compared to a base-catalyzed procedure based on sodium-methoxide in methanol. The influence of the different methods on the relative fatty acid composition (wt% of total fatty acids) and the total fatty acid recovery rate (expressed as % of total lipids) was studied in two experimental LCP-containing formulae and a human milk sample. MeOH/HCl-procedures were found to result in an incomplete transesterification of triglycerides, if an additional nonpolar solvent like toluene or hexane is not added and a water-free preparation is not guaranteed. In infant formulae the low transesterification of triglycerides (up to only 37%) could result in an 100%-overestimation of the relative amount of LCP, if these fatty acids primarily derive from phospholipids. This is the case in infant formulae containing egg lipids as raw materials. In formula containing fish oils and in human milk the efficacy of esterification results in incorrect absolute amounts of fatty acids, but has no remarkable effect on the relative fatty acid distribution. This is due to the fact that in these samples LCP are primarily bound to triglycerides. Furthermore, in formulae based on butterfat the derivatization procedure should be designed in such a way that losses of short-chain fatty acids due to evaporation steps can be avoided. The procedure based on sodium methoxide was found to result in a satisfactory (about 90%) conversion of formula lipids and a reliable content of all individual fatty acids. Due to a possibly high amount of free fatty acids in human milk, which are not methylated by sodium-methoxide, caution is expressed about the use of this reagent for fatty acid analysis of mothers milk. It is concluded that accurate fatty acid analysis of infant formulae and human milk requires a careful and quantitative derivatization of both polar and nonpolar lipid classes. Sodium methoxide seems to be a reliable and time-saving method for routine fatty acid analysis of infant formulae, which should be validated by interlaboratory comparison. Anhydrous procedures based on methanolic hydrogen chloride including an additional nonpolar solvent are also suitable for infant formulae but seem to be preferable for human milk samples.

Chromatography, Gas↗

PubMed1984

Growth and tolerance studies of a new infant formula.

A new routine infant formula has been developed and clinically tested. The clinical study reported here involved 337 normal newborns cared for by six private pediatric group practices. Infants were examined regularly by the investigators until six months of age. Serum biochemistries, hematology, and growth and tolerance variables were compared to infants fed control formulas and to reported values for breast-fed infants. This new formulation compared favorably, in all areas studied here, to controls and previously reported values.

Blood Proteins↗

PubMed1981

Effect of feeding an infant formula with high energy density on gastric emptying in infants with congenital heart disease.

Gastric emptying of meals of standard infant formula and formula fortified with a glucose polymer (Caloreen) were compared in 7 infants with severe congenital heart disease aged 9 days to 5 months. A marker dilution technique was used in estimating gastric emptying. Gastric half-emptying times were significantly longer for meals containing Caloreen than for meals of standard infant formula. Although significantly smaller amount of meal containing Caloreen had left the stomach after 2 hours, the net transfer of energy to the intestine was larger than after feeding a standard meal.

Dextrins↗

PubMed2004

Isoflavones in soy infant formula: a review of evidence for endocrine and other activity in infants.

Soy infant formulas are widely used, but few studies have evaluated long-term safety or examined specific forms of toxicity, such as to the endocrine or immune systems. This review focuses on newer experimental studies of the effects on estrogen activity, immune function, and thyroid economy of genistein and daidzein, two isoflavones in soy infant formula, and existing human studies of soy formula use. In order to judge the likelihood that an endpoint seen in laboratory studies might occur in soy-fed infants, we examined the doses and the resulting serum or plasma concentrations from the laboratory studies and compared them with doses and concentrations seen in soy-fed infants. We also summarized the estimates of the potency of the isoflavone compounds relative to estradiol. Given the scarcity and inconsistency of existing human data and the substantial laboratory evidence of hormonal and other activity at doses relevant to the soy-fed infant, we conclude that more clinical and epidemiological study is warranted.

Dose-Response Relationship, Drug↗

PubMed1995

Effect of a fermented infant formula containing viable bifidobacteria on the fecal flora composition and pH of healthy full-term infants.

We assessed the growth, tolerance, and acceptability as well as fecal flora composition and stool pH of 20 healthy full-term infants fed with a fermented whey-adapted infant formula containing viable bifidobacteria (10(6)/g of powder) during the first 2 months of life. This fermented infant formula, first biologically acidified by Streptococcus thermophilus and Lactobacillus helveticus, was compared to a whey-adapted, nonacidified, low-phosphate infant formula in a double-blind, randomized controlled study. The results were compared to a control group (n = 14) of fully breast-fed infants. The fermented whey-adapted formula containing viable bifidobacteria induced a prevalence of colonization with bifidobacteria at 1 month of age similar to that of breast-fed infants (12/20 versus 8/14) but significantly higher than in the group fed the standard infant formula (4/20). The mean bacterial count of bifidobacteria was similar in all colonized infants; however, fecal pH was significantly lower in the breast-fed infants than in the nonacidified bottle-fed infants. This kind of infant formula was well tolerated and promoted a normal growth during the first 2 months.

Bifidobacterium↗

PubMedDate not supplied

The aluminium content of infant formula and tea.

Tea and infant formulae have been examined for the presence of aluminium. Concentrations in 13 different tea infusions ranged from 2.2 mg/l to 4.5 mg/l. In cow's milk-based infant formulae as made up for consumption, aluminium levels of between 0.03 mg/l and 0.20 mg/l were detected. Higher amounts were present in soya-based formulae with concentrations ranging from 0.64 mg/l to 1.34 mg/l.

Aluminum↗

PubMed1976

Pharmacy-based special infant formula service.

The preparation of infant formulas by a hospital pharmacy for a 280-bed department of pediatrics is discussed. A formulary of acceptable special therapeutic formulas was developed in conjunction with the pediatricians. Space and equipment originally intended for intravenous admixture and total parenteral nutrition services were supplemented to include special infant formula services. The personnel and equipment requirements, and quality control procedures, of the service are discussed.

Formularies, Hospital as Topic↗

PubMed1995

Characterization of antigens and allergens in hypo-allergenic infant formulae.

The antigenicity and allergenicity of so-called hypo-allergenic infant formulae is mainly determined by the degree of hydrolysis and ultrafiltration. Five different formulae were investigated by means of immunoblotting and RAST in order to characterize the antigens and allergens regarding their molecular weights, molecular origin and their ability to bind human IgG and IgE antibodies: A non hydrolysed infant formula (I-F), a mixture of the major cow's milk proteins (PM), a whey-based infant formula (W-H), a whey-based and ultra-filtrated infant formula (U-H), a casein/whey-based infant formula (CW-H). By immunoblotting we demonstrated that all tested formulae still contain antigens with molecular weights from 3 to 67 kD. But when compared with I-F and PM the antigen content of the hydrolysed formulae was considerably lower. The lowest antigen content could be demonstrated in U-H, which contains casein fragments (3-6 kD) and beta-lactoglobulin and its fragments (6-18 kD). W-H and CW-H contain bovine serum albumin, beta-lactoglobulin, casein and their fragments (3-67 kD). All hydrolysed formulae tested showed a reduced IgE-binding capacity. Three out of 12 cow's milk allergic children possessed IgE binding to U-H or W-H, and 5 of them IgE against CW-H. Conclusion. The enzymatic hydrolysis plus ultra-filtration seems to be the most efficient method to reduce the antigen content of so-called hypo-allergenic infant formulae.

Allergens↗

PubMedDate not supplied

Microwave heating of infant formula and breast milk.

Heating infant formula and breast milk in a microwave oven has become a common practice in many households. A review of the literature is presented to ascertain if there is evidence to support the safety of this practice, as well as to determine if microwaving affects the nutritional content of the heated milk. The results of a local community's survey are presented, which assesses parental use of microwave ovens in the heating of infant formula and parents' knowledge of the potential hazards of this practice.

Burns↗

PubMed2001

Solubility and relative absorption of copper, iron, and zinc in two milk-based liquid infant formulae.

The relative solubility of copper, iron, and zinc in two experimental liquid infant formulae are examined. The results of these trials suggest that substituting organic forms of copper and iron in the mix results in an almost three-fold increase in their solubility (iron lactate, 73.4% vs ferrous sulfate, 27.6% and copper gluconate, 11.3% vs cupric sulfate, 3.0%). Organic zinc substitutes did not show this pattern of increased solubility Electron microscopy was employed to document the changes in protein-protein and protein-lipid interactions and to examine the pattern of electron-dense precipitates in the two experimental formulae. Electron micrographs of the liquid infant formula that had been formulated using inorganic salts (sulfates) showed extensive attachment of denatured whey proteins and casein micelles to the oil droplet surfaces and the surface of the oil droplets were also punctuated by electron-dense granule. The oil droplets of the formula produced using organic versions of the mineral salts were smooth and clear of electron-dense deposits. Experiments were designed to determine whether the observed changes in solubility and microstructure were correlated with increases in relative absorption of the minerals. We applied a technique of in vitro acidification followed by a peptic digestion of the two experimental infant formulae with human milk samples as controls. Coupling this in vitro digestion with an absorption model consisting of live isolated intestinal loops from guinea pig we were able to assess the relative absorption of copper, iron and zinc in the test digests. The relative absorption of the three minerals from digests of human milk was significantly higher than for either of the experimental formulae. Relative mineral absorption from digests of the two experimental infant formulae tested was only significantly different (P < 0.05) for Fe. Based on the results from this study we can conclude that substituting organic forms of iron in bovine milk-base infant formulae would have beneficial effects on both the solubility and bioavailability of this important micronutrient.

Analysis of Variance↗

PubMed1994

Pitfalls in the design and manufacture of infant formulae.

The composition of modern infant formulae is basically oriented on the "golden standard" human milk and influenced by several official regulations and recommendations (EC, ESPGAN, etc.). This article will focus on two recent improvements in the field of long-chain polyunsaturated fatty acids (LCPs) and protein hydrolysates. The addition of LCPs for preterm formulae was recommended recently by ESPGAN (the European Society for Pediatric Gastroenterology and Nutrition). Our research has focused on this problem for many years and we have found a good source of LCPs using specially prepared egg-yolk lipids. Others have used fish oils and run into the problem of growth retardation. Therefore, the possible sources of LCPs have to be discussed very critically and the alternatives will be shown. Also, new developments like the use of single-cell oils will be discussed. Second, the use of protein hydrolysates have been introduced for the so-called hypoantigenic or hypoallergenic formulae. Hypoantigenic formulae for preventive use have to be differentiated clearly from hypoallergenic formulae for treatment of proved cows' milk protein allergy. The problems of designing suitable hydrolysates that are low in antigenicity and good in taste will be outlined. The determination of the molecular weight distribution by gel chromatography will be compared critically with the newer techniques. The ELISA technique for testing the antigenicity is recommended before any in vivo evaluation. So far, the anaphylactic guinea-pig model is the most sensitive in vivo testing method. Summing up, modern infant formulae manufacture is much more dependent on modern laboratory techniques, which have to be chosen critically and must be adapted to the newest state of the art.

Fatty Acids, Unsaturated↗

PubMed2002

The U.S. infant formula industry: is direct-to-consumer advertising unethical or inevitable?

Throughout their history, U.S. based infant formula companies have promoted their products as though they required a prescription. This form of marketing is called "ethical" promotion, which focuses on gaining a physician to parent recommendation for a brand of infant formula. Until Nestle's entry into the U.S. infant formula market in 1988, there was little direct-to-consumer promotion of infant formula. This article provides a background on the history of infant formula practices in the United States and then focuses on a descriptive model to explain how mothers' make their infant formula selection. Finally, we offer suggestions for the "ethical" marketers of infant formula.

Advertising↗

PubMed2003

Pesticide content of infant formulae and weaning foods available in New Zealand.

A survey of the pesticide content of 25 commercially available infant formulae and 30 weaning foods available in New Zealand was undertaken in 1996. It included a representative mixture of imported and New Zealand manufactured infant foods. Three different pesticide screening techniques were used: a high-sensitivity organochlorine screen was carried out on all infant formulae, while a multiresidue screen (organochlorine and organophosphorus pesticides, synthetic pyrethroids, carbamate pesticides, fungicides and herbicides), and a specific screen for dithiocarbamate fungicides were both carried out on all weaning foods and on soy-based infant formulae. All results are expressed on a ready-to-feed basis. Extremely low levels of residues of three organochlorine compounds (p,p'-DDE, p,p'-DDT and dieldrin) were detected in infant formulae samples. Residues of p,p'-DDE were found in seven of 20 milk-based infant formulae at concentrations ranging from 0.03 to 0.5 microgram kg(-1). Residues of p,p'-DDT were found in one imported milk-based infant formula at 0.7 microgram kg(-1), and dieldrin residues were detected in four of five soy-based infant formulae at concentrations ranging from 0.05 to 0.08 microgram kg(-1). The multiresidue pesticide screen detected low levels of residues of two organophosphorus pesticides; azinphos-methyl in one soy-based infant formula at a level of 22 microgram kg(-1) and pirimiphos-methyl in two cereal-based weaning foods at concentrations of 5 and 14 microgram kg(-1). None of the other approximately 140 pesticides (including fungicides and herbicides) included in the multiresidue screen were detected in any weaning foods or soy-based infant formulae, at a detection limit of 10 microgram kg(-1). No residues of dithiocarbamate fungicides were detected in any product analysed, at a detection limit of 100 microgram kg(-1).

Azinphosmethyl↗

PubMed2006

Thermal inactivation of ricin using infant formula as a food matrix.

Ricin is a potent protein toxin found in the seeds of the castor bean plant, Ricinus communis. Ricin specifically and irreversibly inactivates ribosomes, promoting cell death by inhibiting protein synthesis. It is composed of a ribosome-inactivating enzyme (A-chain) linked to a lectin (B-chain) by a single disulfide bond. Several reports indicate that ricin can be detoxified by thermal treatment; however, the conditions required for inactivation are not well characterized. In addition, little information exists on the thermal stability of ricin added to foods. The objective of this work was to determine the effects of heat treatments on the detection and toxicity of ricin added to milk- and soy-based infant formulas. Reconstituted infant formula powders containing 100 mug of ricin/mL were heated at 60-90 degrees C for up to 5 h. The heat-treated formulas were analyzed by ELISA to determine levels of ricin. The residual cytotoxicity of ricin-containing infant formula after heat treatments was determined using RAW264.7 mouse macrophage cells. The ELISA and the cytotoxicity assay indicated that ricin detection and toxicity decreased with increasing heating times and temperatures. Minimal losses in detection and toxicity were found for ricin heated at 60 degrees C for 2 h. The half-lives of ricin cytoxic activity in a milk-based infant formula at 60, 70, 75, 80, 85, and 90 degrees C were >100, 9.8 +/- 0.5, 5.8 +/- 0.9, 5.1 +/- 0.7, 3.1 +/- 0.4, and 1.8 +/- 0.2 min, respectively; the comparable values for a soy-based infant formula were >100, 16 +/- 1.6, 8.7 +/- 1.2, 6.9 +/- 1.1, 3.0 +/- 0.4, and 2.0 +/- 0.3 min. ELISA detection was a good indicator of the cytotoxicity of heat-treated ricin. The results indicate that ricin is a relatively heat stable protein and may remain toxic under some food processing conditions.

Animals↗

PubMed2006

True ileal amino acid digestibility of goat and cow milk infant formulas.

Goat milk is used as an alternative to cow milk for the production of infant formulas. However, little is known about the protein quality and, specifically, about the digestible AA pattern of goat milk formulas compared with their cow milk counterparts. In this study, the true ileal AA digestibility of a goat milk infant formula was compared with a premium cow milk infant formula. The 3-wk-old piglet was used as a model for the 3-mo-old infant. Both milk formulas were prepared as described by the manufacturer, with titanium dioxide added as an indigestible marker. The formulas were fed to the piglets over a 2-wk trial period. Digesta from the terminal ileum were collected post euthanasia and analyzed for AA content, along with samples of the formulas. True AA digestibility was determined after correcting for endogenous AA loss at the terminal ileum of pigs fed an enzyme-hydrolyzed casein-based diet, followed by ultrafiltration (5,000 Da) of the digesta. Total urine and feces collection was also undertaken to determine the nitrogen retention from the diets. The true ileal AA digestibility was similar between the goat and cow milk infant formulas for all AA except Gly and Trp. There was no significant difference in the nitrogen retention of piglets fed the two different formulas. The goat milk infant formula and the premium cow milk infant formula were similar in terms of protein quality.

Amino Acids↗

PubMed2004

Iron bioavailability from powdered and in-bottle-sterilized infant formulas in suckling and weanling rats.

OBJECTIVE: The Maillard reaction and lactose isomerization may be induced during the manufacture of infant formulas. We studied the effects of dehydration and sterilization on iron bioavailability from an infant formula in suckling and weanling rats. METHODS: In experiment 1, a previously reconstituted powdered infant formula and an in-bottle-sterilized liquid infant formula from the same manufacturer were fed from drinking bottles to 2-wk-old suckling rat pups for 7 d. In experiment 2, the same formulas were complemented with AIN-76 and fed to weanling rats for 7 d after a 4-d adaptation period. In both experiments, intake, body weight, and fecal and urinary excretions were monitored, and the following iron indexes were calculated: apparent absorption and retention and the coefficients percentage of absorption versus intake, percentage of retention versus absorption, and percentage of retention versus intake. RESULTS: The liquid infant formula resulted in lower body weights on day 4, particularly among the younger rats that had significantly lower food intakes (P = 0.045). In weanling rats fed powdered and liquid infant formulas, food intake and body weight were not significantly different. The pups showed significantly higher absorption (percentage of absorption versus intake) and retention (percentages of retention versus absorption and retention versus intake) efficiencies than did the weanling rats (P < 0.001 for the three indexes). Hemoglobin values (P = 0.001) and liver iron concentrations (P = 0.009) were significantly higher in the weanling rats than in the pups. In contrast, erythrocyte iron concentrations and hematocrit were higher in the pups (P = 0.016 and 0.053, respectively). CONCLUSIONS: In rat pups, iron bioavailability is negatively affected by the consumption of in-bottle-sterilized infant formula, possibly as the result of the content of Maillard reaction products, altered proteins, and lactulose. However, when this formula is included in a mixed diet and given to weanling rats, this detrimental effect no longer occurs.

Analysis of Variance↗