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The Limulus test and bacterial endotoxins: some perspectives.
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[Preliminary clinical observations on the detection of gram-negative bacterial meningitis by limulus test].
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Limulus test, parenteral drugs and biological products: an approach.
The Authors report the results concerning the control of presence of bacterial endotoxins in parenteral drugs, blood products and immunological materials, by means of Limulus amebocyte lysate test. All the data suggest that Limulus assay is a simple, rapid, specific and sensitive test for endotoxin detection, mostly when it is correctly performed.
Limulus test, parenteral drugs and biological products: an approach.
The Authors report the results concerning the control of the presence of bacterial endotoxins in parenteral drugs, blood products and immunological reagents and drugs, by means of Limulus amebocyte lysate test. All the data suggest that Limulus assay is a simple, rapid, sensitive test, mostly when it is correctly performed.
Investigations on an essential plasma factor disturbing the photometric determination of endotoxin in plasma samples with the Limulus test.
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[Preliminary results of detecting endotoxin-like substances in Chlamydia trachomatis by the Limulus test].
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[Specificity of the limulus test for the detection of endotoxins].
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[The Limulus test in microbiological quality control].
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Clinical utility of the Limulus test with blood, CSF and synovial fluid.
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Acceptance of the Limulus test as an alternative pyrogen test for radiopharmaceutical and intrathecal drugs.
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A minor improvement of the Limulus test.
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[Existence of glucan-like activity in the organs of rats. Causes of postoperative elevations of endotoxin using conventional limulus test (toxicolor). Preliminary report].
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[Limulus test for the control of water and materials used in injectables (author's transl)].
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[Limulus test (L.T.) of urine in the diagnosis of urinary tract infection (author's transl)].
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Polyclonal B cell activation, endotoxin tolerance, and limulus tests of endotoxin preparations of some periodontopathogens.
Potencies of polyclonal B-cell activation in C3H/HeN mice of Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, and Porphyromonas gingivalis endotoxins were 0.36, 0.13 and 0.04, taking Salmonella abortusequi as 1.0. F. nucleatum and P. gingivalis endotoxins showed positive reactions in C3H/HeJ mice. Most activities in C3H/HeN other than that of F. nucleatum were suppressed by polymyxin B. In C3H/HeJ mice, similar inhibitions were only 60% for P. gingivalis and hardly observed with F. nucleatum. The resistances to polymyxin B could be due to protein in the endotoxins. A promoting effect of T cells added to B cells was observed only in the activity of F. nucleatum endotoxin in C3H/HeJ mice; there was no influence in other groups. Test endotoxins had nearly the same ability to produce colony stimulating factor as did references and could not produce the factor in tolerant mice. The clinical significance of tolerance is discussed. Regression lines of endotoxin doses and limulus activities of test endotoxins and Salmonella were parallel, either in specific or non-specific tests. The lines of two test groups were also parallel; values obtained by two tests were very close. These data indicate that the test endotoxins did not contain (1-3)-beta-D-glucan and elicited qualitatively similar limulus reactions to that of the reference, despite their different chemical natures. In conclusion, these test preparations had an endotoxicity similar to that of the reference and contribute to produce periodontitis through polyclonal B cell activation.
[The Limulus test in the diagnosis of endotoxemia].
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[Limulus test: rapid spectrophotometric determination of endotoxin].
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