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At least 91 records · Page 5Linked to original sources
PubMed1978

Xenogenic response of human peripheral blood lymphocytes against murine cells in vitro.

Human peripheral blood lymphocytes (HPBL) were sensitized to mitomycin-treated murine spleen cells and mitomycin-treated mastocytoma P-815. A DNA proliferative response occurred to both cell types. The sensitized HPBL lysed chromium-51-labelled mastocytoma P-815 cells in a cell-mediated cytotoxicity assay (CMC). Evidence is presented that this human-murine xenogeneic system involves T cell response with specificity in CMC mainly toward H-2 antigens. DBA/2 splenocytes were superior to mastocytoma cells as sensitizers in the xenogeneic DNA proliferative assay.

Animals↗
PubMed1979

A radio (51Cr) micro-tube leukocyte adherence inhibition assay: specific tumor-associated immunity in 3 murine tumor systems.

A radio (51Cr) micro-tube leukocyte adherence inhibition assay is described. In this study, murine mononuclear cells were labeled with 51Cr, plated into tissue culture plates with different tumor extracts and counts/min (cpm) of the non-adherent cells were used as a parameter of adherence inhibition. This assay was used to measure anti-tumor immunity, in vitro, in 3 murine tumor systems: MCA-38 colon adenocarcinoma, L1210 lymphoma and P815 mastocytoma. Tumor immunity was detected using 3 doses (0.01-0.001 mg/ml) of tumor extract in the MCA-38 tumor model, and using 2 doses (0.1-0.05 mg/ml) of tumor extract in both the L1210 and P815 tumor models. It was observed that specific tumor-associated adherence inhibition could be measured in the MCA-38 tumor model between days 7 and 22 of tumor growth and in the L1210 and P815 tumor models between days 7 and 17 of tumor growth. The radio-LAI assay described is an easy, specific and reproducible way to measure tumor-associated adherence inhibition, in vitro.

Adenocarcinoma↗
PubMed1979

Colloidal silica--aluminum modified--PVP density gradient centrifugation: centrifuge tube wall cell adherence, aggregation, separation properties and comparison to BSA and Ficoll.

A method is described for the inexpensive and easy preparation of colloidal silica--aluminum modified--polyvinylpyrrolidone (CS-AM-PVP) density gradient centrifugation medium. Using density gradient centrifugation, several cell separation and biochemical characteristics were studied: centrifuge tube wall cell adherence, mouse spleen cell density distribution, rebanding properties, mitogen response and cell aggregation. Cell adherence to the centrifuge tube wall using density gradients of CS-AM-PVP was compared with density gradients of bovine serum albumin and Ficoll. Few cells adhered to the centrifuge tube wall when CS-AM-PVP was used as a gradient medium; whereas, significant cell adherence to the centrifuge tube cell wall occurred when bovine serum albumin or Ficoll was used as a gradient medium. The CS-AM-PVP gradient medium did not inhibit the response to mitogens of mouse spleen cells which had been separated into density subpopulations in a discontinuous CS-AM-PVP density gradient, caused a minimum amount of cell aggregation, and was found to be non-toxic.

Aluminum↗
PubMedDate not supplied

A multiple-parameter comparison of immunocompetence and tumor resistance in aged BALB/c mice.

Several parameters of cell-mediated and humoral immunity were measured in young-adult and aged BALB/c mice and compared with resistance to the ascites form of intraperitoneal induced P815 mastocytoma. It was found that the age-related decline of in vitro phytohemagglutinin (PHA) responsiveness by spleen cells approximated the marked decrease of old mice to tumor-cell challenge and approached that characteristically seen in humoral immunity. Thus, decreased PHA responsiveness of splenic lymphocytes provided as sensitive an estimate of the age-related decline of immunocompetence in old mice as other classical parameters of cell-mediated immunity (e.g. graft-vs-host reaction or in vivo cellular proliferation of parental spleen cells in lethally-irradiated F1 recipients). Results could be interpreted to represent a decreased ability of noncycling T-cells to be released to a functional cycling state.

Aging↗
PubMed1975

A model of cell cycle control: effects of thymidine on synchronous cell cultures.

Further evidence is presented in support of a model for growth control in which commitment for cell division is determined by an event in the preceding cell cycle. A study was made of conditions affecting synchronous growth following treatment of murine mastocytoma cells with excess thymidine at different phases of the cell cycle. Cells were synchronized by a physical procedure involving velocity sedimentation in a zonal rotor. Pulse treatment of such cultures with thymidine at times corresponding to the S, G2, and M periods had no effect on further growth. However, addition at G1, although having no immediate effect, arrested cell growth in the next cell cycle. This temporal effect may account for the decay of synchrony observed during double thymidine blockade or thymidine-FUdR blockade. When the time interval between two such blocks was 7 hr or less, P815Y cells were arrested after one synchronous division. At this critical time a majority of cells were at, or near, G1. It is suggested that thymidine exerts a hitherto unrecognized effect at the G1 interval.

Animals↗
PubMed1979

Spin labeling studies of the interactions of P815 mouse mastocytoma cells with antiserum to histocompatibility antigens, H-2d.

The effect of antiserum to histocompatibility antigens on the membrane organization of murine neoplastic mast cells (P815) has been studied by spin labeling. Incubation of in vivo passaged cells, labeled with a nitroxide derivative of methyl stearate, with antiserum to membrane carried histocompatibility antigens, H-2d, resulted in an apparent decrease in membrane fluidity that was accompanied by histamine release. This electron spin resonance (esr) detectable change was found to be temperature, time, and dose dependent. Treatment of the cells with cytochalasin B, a drug disrupting microfilament structure, inhibited the effects induced by H-2d antiserum. In contrast, vinblastine, which disrupts microtubules, did not modulate the effect of this antiserum. Other data presented here suggest that some of the large spectral change observed on treatment of the cells with anti-H-2d serum may result from hydrolysis of the spin label fatty acid ester via activation of a membrane-associated esterase. The relationship of these results to other immunologically induced membrane phenomena is discussed.

Animals↗
PubMedDate not supplied

The influence of anti-theta-globulin treatment on the growth of mastocytoma in mice.

DBA/2 mice were treated with four successive subcutaneous injections of rabbit anti-theta-gamma-globulin followed by the subcutaneous implantation of chemically induced mastocytoma (P-815-X2). Another series of animals received rabbit anti-thymus-gammaglobulin according to the same schedule and still another series of animals served as non-treated controls. A definite augmentation of the tumor growth, as evidenced by the dissemination of the tumor into the spleen, liver and kidney, was evident in the globulin-treated series. Such dissemination was not observed in the control animals, where the metastases were limited to the reginal lymph nodes. The studied gammaglobulins were different in two important respects; the death rate of animals and the frequency of thymic metastases were higher in the anti-theta-globulin series. These findings advocate the conclusion that anti-theta-globulin, prepared against the brain tissue, is the more specific and more potent T-lymphocyte suppressor of these two globulins studied. T-lymphocyte population seems to play an important role in host resistance against experimental neoplasia.

Animals↗
PubMed1978

Post-capillary venules in the lymphatic tissues of mice bearing experimental neoplasia.

The lymph nodes, thymus and Peyer's patches of DBA/2 mice bearing an experimental tumor, mastocytoma, were assessed histologically with special reference to the structure of the post-capillary venules. For each of the lymphoid organ studied, the post-capillary venule score (PCV-S) was determined on the three grades (grades 1, 2 and 3) of the venules classified according to the height of the endothelial cells. The highest scores were obtained in the lymph nodes and Peyer's patches of the control animals. The scores in these lymphoid organs of the tumor-bearing mice were statistically highly significantly lower than in the control series. The lowest scores, however, were obtained in the nodes and patches of mice bearing mastocytoma after the previous treatment with anti-theta-globulin. The scores in the thymuses did not deviate from each other in the three series of mice studied. The findings of the present work support the concept that the structural state of the post-capillary venules in the lymph nodes and Peyer's patches is an important regulator of the T-lymphocyte recirculation in these organs. On the other hand, the venules of the thymus seem to be unrelated both structurally and functionally to the post-capillary venules of the nodes and Peyer's patches, and a new name of "junctional venules" has been proposed for these low endothelium walled venules of the thymus.

Animals↗