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Polarizing microscopy and microradiography of sound enamel.

Plano-parallel longitudinal sections from 19 sound human premolars were imbibed in water and in air. The total birefringence of the occlusal, the midcoronal and the cervical region of the buccal sound enamel cap was then determined. The volume percentage mineral was measured in the same areas using semi-quantitative microradiography. The results show that when the mean values of both water and air imbibed occlusal and midcoronal enamel are considered, there is a positive correlation between the observed total birefringence and the mineral content. The cervical, fissure and cuspal regions showed a poor correlation between mineral content and total birefringence. In longitudinal sections, the influence of the structural organization and the organic matrix is not marked in the occlusal and midcoronal regions although the cervical, fissure and cuspal regions are probably influenced to the greatest extent by these factors.

Air↗

Metallographic texture analysis of dental materials by polarizing microscopy in the incidental light.

Incidental polarized light microscopy can be used for the optical analysis of crystal phases with similar anisotropic behavior or preferred textures in polished sections. In this method, the vibration mode of the reflected light is determined by remarkable interference colors in the microscope using a compensator plate. The application of this method for the analysis of dental materials is demonstrated with amalgams and gold-reduced alloys. As a prerequisite, a modified procedure for preparing suitable polished sections of inhomogenous materials is described. Employing this optical and a crystallographic computing method, more detailed information about the way of element replacement in the course of the chemical reaction can be obtained. The results of these investigations also warrant the assumption that coherent crystal intergrowth which prefers low indicated and closely packed lattice directions, gives rise to a good adhesive strength of the bonding in the alloy.

Crystallography↗