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At least 91 records · Page 5Linked to original sources

The NMR-mouse: construction, excitation, and applications.

A mobile nuclear magnetic resonance (NMR) device similar to a bore-hole probe has been developed for applications in materials science and biomedicine. Inhomogeneous polarizing and radio-frequency (rf) magnetic fields are applied to arbitrarily large samples from one side. Different experimental techniques have been tested to measure transverse and longitudinal relaxation times and translational diffusion constants. Good contrast for discrimination of material properties is gained when the residual dipolar coupling is retained in soft matter by avoiding spinlock effects in multi-pulse techniques. Applications to characterization of products from technical elastomers, skin, and coatings on iron sheets are reported.

Animals↗

[Relatively limited utilization of transportation facilities for handicapped by allochthonous handicapped subjects in The Netherlands].

In order to check whether immigrants and nationals in the Netherlands behave differently in connection with the application for mobile facilities for handicapped people, a retrospective, comparative study was carried out in The Hague and Rotterdam, where more than 20% of the immigrants reside. A total of 600 applicants for mobile facilities in 1986 were selected at random, 300 from Rotterdam and 300 from The Hague; based on the surname of the applicant, the latter was considered to be either an immigrant or a national. Mobile facilities are the most frequently provided facilities. The division between the different nationalities involving the applications was derived from the demographic figures of both cities. Moreover various characteristics of a minor group of immigrant and national applicants for mobile facilities were analysed. In both cities fewer immigrants had applied for a mobile facility than might have been expected on the basis of the demographic figures. To substantiate this, a similar study was made of a file on 1477 clients, who had a mobile facility on loan from the Community Health Service in The Hague. Fewer immigrants than might have been expected on the basis of the demographic figures, had a mobile facility on loan. Immigrants, compared to nationals, are lagging considerably behind with respect to using mobile facilities from the facility package of the General Disablement Act (p less than 0.05). It was noticeable from the characteristics of the applicants and users of mobile facilities, that applications from immigrants for a mobile facility were mostly submitted by an official of a convalescence institute. Applications from immigrants mostly involved young people suffering from neurological disorders. National applicants and users of a mobile facility mostly submitted the application themselves; they had a higher average age and often suffered from neurological disorders or diseases of joints and muscles.

Adult↗

A mobile computed tomographic scanner with intraoperative and intensive care unit applications.

INTRODUCTION: A mobile computed tomographic scanner has been developed in which the scan plane is selected by means of gantry translation, rather than by translation of the patient table. This permits computed tomographic scanning in situ of any patient who is positioned on a radiolucent surface that fits within the inner diameter of the gantry. We report the design of and initial experience with this scanner as used with adapters for intraoperative and bedside computed tomography (CT). METHODS: The scanner is equipped with wheels, draws power from wall outlets (120 V, 20 A) in combination with batteries, and has a translating gantry. Preclinical studies of image quality were performed with phantoms. An operating table adapter was built for use with a radiolucent cranial fixation device. A bedside adapter was built that holds the head and shoulders of a patient in the intensive care unit. RESULTS: The preclinical phantom studies showed satisfactory image spatial resolution (0.8 mm) and low-contrast resolution signal-to-noise relative standard deviation (0.37%). Experience to date with 12 patients has confirmed the feasibility of intraoperative CT on demand. Experience to date with 26 patients has confirmed the feasibility of routine bedside CT in the intensive care unit. CONCLUSION: With these adaptations, mobile CT may increase the efficiency of intraoperative scanning by making it available to multiple operating rooms without committing it to any room for an entire operation and may increase the efficiency and safety of CT of critically ill patients who currently need to leave the intensive care unit to travel to a fixed CT installation and back.

Child↗

Not in our back yard: media coverage of community opposition to mobile phone towers--an application of Sandman's outrage model of risk perception.

Australia has the world's second highest per capita use of mobile phones. To meet rapid expansion in use, transmission towers are being erected hastily, backed by federal government exemptions from normal local government planning procedures. In a case study of media reportage of a successful resident protest about the placement of transmitters adjacent to a kindergarten, uncertain risks to health were the ostensible concerns voiced by the protesters. Eleven of the 12 primary components and six out of eight secondary components of Sandman's model of community risk perception were consistent with both the protesters' expressed concerns about the transmission towers and the way in which the issues involved were framed by the media.

Attitude↗

Reliability of a functional mobility assessment tool with application to neurologically impaired patients: a preliminary report.

The purpose of this preliminary study was to examine the intratester and intertester reliability of a functional mobility assessment tool (FMAT). Seven licensed physical therapists with varying amounts of clinical experience served as raters. Twelve patients with neurological deficits were subjects for this study. Raters were asked to provide eight possible ratings for each of eight critical mobility functions. Average weighted kappa coefficients ranged from .82 to .97. Intraclass correlation coefficients ranged from .73 to .97 for the first assessment and from .52 to .97 for the second (retest) assessment. A high degree of agreement between and within seven raters indicated that this tool may provide an effective assessment of stroke, brain injury, and spinal cord injury. Preliminary findings indicate the functional mobility assessment tool is reliable, thereby increasing the usefulness of this method for clinical assessment. The high resolution of the FMAT makes this tool ideally suited for use in future studies focusing on the prediction of mobility function following neurological insult.

Activities of Daily Living↗

Clinical applications of a standardized mobility test.

Four patients with mobility problems were evaluated in relation to their performances on a standardized mobility test throughout the course of their rehabilitation programs. While each of the four cases demonstrates the value of an objective measurement of independent function, each also reflects individual points of merit. Clinical application demonstrated the test's ability to separate out areas of mobility deficit, evaluate forms of treatment, and indicate need for follow-up. Further, the sensitivity of the test in long-term follow-up of mobility problems was illustrated.

Adult↗

Model for Plateau border drainage of power-law fluid with mobile interface and its application to foam drainage.

A model for drainage of a power-law fluid through a Plateau border is proposed which accounts for the actual Plateau border geometry and interfacial mobility. The non-dimensionalized Navier-Stokes equations have been solved using finite element method to obtain the contours of velocity within the Plateau border cross section and average Plateau border velocity in terms of dimensionless inverse surface viscosity and power-law rheological parameters. The velocity coefficient, the correction for the average velocity through a Plateau border of actual geometry compared to that for a simplified circular geometry of the same area of cross section, was expressed as a function of dimensionless inverse surface viscosity and flow behavior index of the power-law fluid. The results of this improved model for Plateau border drainage were then incorporated in a previously developed foam drainage model [G. Narsimhan, J. Food Eng. 14 (1991) 139] to predict the evolution of liquid holdup profiles in a standing foam. Foam drainage was found to be slower for actual Plateau border cross section compared to circular geometry and faster for higher interfacial mobility and larger bubble size. Evolution of liquid holdup profiles in a standing foam formed by whipping and stabilized by 0.1% beta-lactoglobulin in the presence of xanthan gum when subjected to 16g and 45g centrifugal force fields was measured using magnetic resonance imaging for different xanthan gum concentrations. Drainage resulted in the formation of a separate liquid layer at the bottom at longer times. Measured bubble size, surface shear viscosity of beta-lactoglobulin solutions and literature values of power-law parameters of xanthan gum solution were employed in the current model to predict the evolution of liquid holdup profile which compared well with the experimental data. Newtonian model for foam drainage for zero shear viscosity underpredicted drainage rates and did not agree with the experimental data.

Journal Article↗

The mobile receptor hypothesis and "cooperativity" of hormone binding. Application to insulin.

The mobile receptor hypothesis has been proposed to describe the process by which hormone receptor binding initiates a biological response; it states that receptors, which can diffuse independently in the plane of the membrane, reversibly associate with effectors to regulate their activity. The affinity for effector is greater when the receptor is occupied by hormone. A mathematical expression of the mobile receptor hypothesis is used to show that: (1) The predicted kinetics of hormone receptor binding may be indistinguishable from "negative cooperativity." (2) Receptor occupancy and biological response may be coupled in a non-linear fashion. By choosing specific parameters, most of the existing data on insulin binding and biological responses can be explained in terms of the mobile receptor hypothesis. Thus, the following are easily explained: (1) A single homogeneous receptor may appear kinetically to be composed of two classes (of high and low affinity) of receptors. (2) Occupancy of the apparent class of high affinity receptors is related linearly to the biological response. (3) The same receptor in different tissues may appear to have different affinity. (4) The binding of different biologically active insulin analogues may exhibit different degrees of "cooperativity." These considerations may also be pertinent to interpretations of other hormone-receptor systems and of various ligand-macromolecule interactions.

Binding Sites↗

Joint mobilization principles. Considerations for use in the child with central nervous system dysfunction.

The purpose of this article is to explore the use of mobilization in the treatment of capsular dysfunction of the shoulder joint in children with central nervous system dysfunction. A review of functional anatomy and of normal and abnormal development of the shoulder is presented. Basic mobilization principles and application of joint mobilization techniques to children with long-standing shoulder hypomobility and spasticity are discussed. Research to determine the benefits and risks of mobilization of joints in children with spasticity is recommended.

Central Nervous System Diseases↗

A semi-empirical approach to the modeling of the electrophoretic mobility in free solution: application to polystyrenesulfonates of various sulfonation rates.

This work focuses on the understanding of the electrophoretic behavior of flexible chains of polystyrenesulfonates (PSSs) in free solution. It deals mainly with the variation of the electrophoretic mobility with (i) the polymerization degree (N) of fully sulfonated PSSs and (ii) the sulfonation rate of randomly sulfonated PSSs. In both cases, the electrophoretic mobility was modeled following a semi-empirical approach which involves parameters retaining a physical meaning. Fully sulfonated PSS oligomers, having a length smaller than or similar to the Debye length, exhibit a particular electrophoretic behavior, in-between that observed for multicharged small molecules and that for polyelectrolytes. The electrophoretic mobility of these oligomers increases strongly with N, which is attributed to a hydrodynamic coupling between monomers. Then the mobility is maximum for an N of about 10, for which the PSS oligomers are still in a rod-like conformation. Afterwards, as N increases and the PSSs are larger than the Debye length, the electrophoretic mobility decreases slowly until it reaches a constant value corresponding to the free-draining behavior. Next, the electrophoretic behavior of long PSS (N about 1,200) differing in their sulfonation rates was investigated. The effective charge rates were determined independently by conductimetric measurements and the mobilities were modeled as a function of the sulfonation rate. The PSS behavior observed was compared to the one previously reported for classical polyelectrolytes having hydrophilic backbones, such as copolymers of poly(acryamide-coacrylic acid). A specific behavior has been pointed out for these partially sulfonated PSSs, which is attributed to the hydrophobicity of their backbone. Finally, it is shown that separations of PSSs of different sulfonation rates can be obtained with electrolytes containing an anionic surfactant or methanol.

Electrophoresis↗

Protein-directed DNA structure. I. Raman spectroscopy of a high-mobility-group box with application to human sex reversal.

Protein-directed reorganization of DNA underlies mechanisms of transcription, replication, and recombination. A molecular model for DNA reorganization in the regulation of gene expression is provided by the sequence-specific high-mobility-group (HMG) box. Structures of HMG-box complexes with DNA are characterized by expansion of the minor groove, sharp bending toward the major groove, and local unwinding of the double helix. The Raman vibrational signature of such DNA reorganization has been identified in a study of the SRY HMG box, encoded by the human male-determining region of the Y chromosome. We observe in the human SRY-HMG:DNA complex extraordinarily large perturbations to Raman bands associated with vibrational modes of the DNA backbone and accompanying large increases in intensities of Raman bands attributable to base unstacking. In contrast, DNA major-groove binding, as occurs for the bZIP protein GCN4 [Benevides, J. M., Li, T., Lu, X.-J., Srinivasan, A. R., Olson, W. K., Weiss, M. A., and Thomas, G. J., Jr. (2000) Biochemistry 39, 548-556], perturbs the Raman signature of DNA only marginally. Raman markers of minor-groove recognition in the human SRY-HMG:DNA complex are due primarily to perturbation of specific vibrational modes of deoxyribose moieties and presumably reflect desolvation at the nonpolar interface of protein and DNA. These Raman markers may be diagnostic of protein-induced DNA bending and are proposed as a baseline for comparative analysis of mutations in SRY that cause human sex reversal.

Amino Acid Sequence↗

Development, feasibility, acceptability, and preliminary impact of NutriSOS®: A behavioral mobile app to promote sustainable diets.

The primary objective of this study was to describe the development of the NutriSOS&#xae; app and to evaluate its feasibility and acceptability for its use in the NutriSOS&#xae; Randomized Controlled Trial (RCT) to promote sustainable diets. A secondary objective was to explore preliminary changes in dietary and physical activity behaviors and environmental impact following app use. The NutriSOS&#xae; app integrates personalized dietary advice, educational content, self-monitoring, and social interaction features. A single-arm, pre-post pilot study was conducted in 37 young Mexican adults over four weeks. Feasibility, acceptability, quality, and usability were assessed using online surveys, alongside exploratory changes in dietary and physical activity behaviors, environmental indicators, and their association with perceived behavioral determinants. Feasibility and acceptability were high overall, with favorable responses reaching up to 100% in key components such as the nutritional guide and learning modules, and above 90% for messaging, registration, and design. Greater variability was observed in some sections, particularly the 24-h recall (41-86%). Reductions in red and processed meat and ultra-processed food consumption were observed (from 3 to 1 times/week, p&#xa0;<&#xa0;0.01), with &#x223c;60% decreases in their related environmental footprints (p&#xa0;<&#xa0;0.01) and favorable self-reported behavioral determinants (p&#xa0;<&#xa0;0.0001). Physical activity type and intensity changed (p&#xa0;<&#xa0;0.05). These findings support NutriSOS&#xae; as a feasible and acceptable tool, while highlighting areas for refinement, particularly those related to the time and effort required for data entry, prior to its implementation in the NutriSOS&#xae; RCT, in which its effectiveness will be formally evaluated.

Humans↗

Ferguson plots based on absolute mobilities in polyacrylamide gel electrophoresis: dependence of linearity of polymerization conditions and application to the determination of free mobility.

In contrast to Ferguson plots based on relative mobilities, Ferguson plots of proteins in polyacrylamide gel electrophoresis based on their absolute mobilities were found to be linear under unusual polymerization conditions which yield relatively wide gel fibers and a low total fiber length per unit weight, but not under previously and commonly used conditions. These linear Ferguson plots in gels of 1, 3 and 5% crosslinking intersect at a single gel concentration between 1 and 2% T (M-point). It is postulated that the measure of free mobility of the proteins is the M-point, and not the intercept of their Ferguson plots with the mobility axis as assumed previously. This postulate abolishes the well-known paradoxical interpretation of the increase with %C of the linearly extrapolated intercept of the Ferguson plot with the log(mobility) axis (designated Yo) in terms of free mobility. The postulate is also compatible with the interpretation of the points of intersection of the Ferguson plots of oligomeric series of proteins at finite gel concentrations (designated mu-points) as their common free mobilities.

Buffers↗

Application of the heteroduplex mobility assay (HMA) for the investigation of the genomic diversity among noroviruses in environmental samples.

Recent studies have demonstrated the widespread contamination of river and seawater with noroviruses (NV), often with more than one strain. The heteroduplex mobility assay (HMA) in which amplicons from study samples are hybridised (by denaturing and reannealing) to amplicons from reference strains and resolved by electrophoresis, has the potential to provide a simple and rapid means to identify samples containing multiple NV strains and to establish the diversity of strains within that sample. PCR amplicons from environmental samples that were tested directly in the HMA assay were shown to contain more than one strain. In order to evaluate HMA for investigations of NV diversity in environmental samples, amplicons from three representative samples were cloned and, for each, 20 amplicons derived from individual clones were analysed by HMA. Between two and six different HMA profiles were demonstrated among clones from a single sample indicating the extent of NV diversity in the sample. Sequence analysis confirmed the relationship of HMA profile and NV 'genotype'. Far greater diversity was seen among Genogroup (G) II (Ni/E3) amplicons than Genogroup (G) I (Ando/E3) amplicons (generated from the RNA dependent RNA polymerase region of the ORF1 of noroviruses), which often contained only a single strain, which is reflective of the greater prevalence of GII NVs over GI NVs. Overall, four GII and four GI strains were identified in these environmental water/sewage samples.

Base Sequence↗