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At least 91 records · Page 5Linked to original sources
PubMed2001

PREP for peds--patient physiology, rescuer responses, equipment, protocols. Size-up & approach tips for pediatric calls.

Well-orchestrated EMS calls demand a global size-up followed by a focused assessment and treatment plan. Use the PAT to rapidly answer the questions, "How sick?" and "How quick?" Knowledge of the child's physiological status allows you to make appropriate decisions about triage, how you should react, what equipment to prepare and what treatment and transport protocols to use. The PREP scheme gives you a consistent and reliable approach for every pediatric call, facilitating focused management for an unstable child and a more relaxed and confident encounter for the stable child and their family.

Child↗
PubMed2003

Altering the course of marriage: the effect of PREP communication skills acquisition on couples' risk of becoming maritally distressed.

This longitudinal study predicted marital outcome from communication skills gained from participation in the Prevention and Relationship Enhancement Program (PREP; H. J. Markman, S. L. Blumberg & S. M. Stanley, 1989; Stanley et al., 2001) by 39 women and 38 men. Results were in the expected direction for men but not for women. Men's pre- to posttest decrease in negative communication and increase in positive communication predicted decreased risk of marital distress onset in men. Women's pre- to posttest increase in positive communication, which was related to self-reported avoidance of problem solving, predicted an increased risk of male and female distress onset. Couples with men relatively high on premarital risk factors demonstrated a greater benefit from men's positive communication skills acquisition.

Adult↗
PubMed1990

Prep-A-Gene: a superior matrix for the purification of DNA and DNA fragments.

A new chromatographic matrix, Prep-A-Gene, is described for the isolation and purification of high quality DNA suitable for restriction analysis, ligation, transformation and sequencing protocols. This matrix selectively binds DNA greater than approximately 200 base pairs in length, while RNA, proteins, cellular components, agarose and other contaminants are washed free in minutes. This eliminates the need for time-consuming and laborious RNase treatments, gel extractions and phenol extractions. The DNA that is desorbed from the matrix is available immediately as a substrate for subsequent protocols. DNA purified in this manner exhibits no detectable shearing, even with more fragile chromosomal DNA.

Animals↗
PubMed1975

[The prep-trainer--thermal control in the pulp space].

The "Prep-Trainer" was developed as teaching and checking aid in order to enable students and dentists interested in continued education to check themselves. The heat development in the pulp cavity is measured at the model. Measurements made by the author showed that the safest method for maintaining constant temperatures and thus avoiding over-heating of the pulp cavity is the cooling with the air-water spray handpiece by the dental assistant.

Audiovisual Aids↗
PubMed1982

A rapid isolation technique for drugs from tissues and fluids: use of the Du Pont Prep 1 system.

A microprocessor-controlled, automated extraction/concentration device, the Prep 1 (Du Pont Clinical Systems), was evaluated for application to the isolation of drug substances from postmortem fluids and tissue homogenates. Two classes of materials were investigated: barbiturates and the benzodiazepine, diazepam. With as little as 200 mg of tissue, barbiturate derivatives were successfully isolated and measured by gas chromatography using nitrogen-phosphorus detection with a coefficient of variation of 2 to 5%. Diazepam was measured in a similar fashion with a coefficient of variation of 4.4%. Preliminary investigation indicates that this system is applicable to a wide range of drug substances of toxicological interest.

Autopsy↗
PubMed1995

PREP profiles: creating and using learning opportunities.

PREP requires practitioners to demonstrate that they have completed the equivalent of five days' study activity over three years. This requirement commences from the first renewal of registration with the UKCC after April 1995. Study requirements may be fulfilled in many different ways, without necessarily spending time in formal classroom courses. Profile records should include outcomes, demonstrating the relevance of the study activity to the practitioner's work area.

Career Mobility↗
PubMed1995

The implications of PREP for practitioners.

The UKCC's proposals for PREP take effect on April 1, 1995. There are three major proposals: preceptor support; maintenance of effective registration and standard-setting for post-registration courses. There are four requirements for maintaining effective registration: notification of practice; return to practice programmes; five days of study every three years; and professional profiling.

Education, Nursing, Continuing↗
PubMed1995

The new PREP requirements.

The UKCC post-registration education and practice (PREP) regulations come into effect this month. HVA education officer Sarah Forester answers some of the questions commonly raised by members about the new requirements for renewal of registration.

Education, Nursing, Continuing↗
PubMed1997

PREP: what's on offer?

To help you maintain and develop your professional and clinical practice and meet your UKCC requirements for PREP the RCN is offering free Continuing Education vouchers along with some new CE initiatives starting next week in Nursing Standard. This report explains what will be available.

Education, Nursing, Continuing↗
PubMed2002

Prep2: cloning and expression of a new prep family member.

We describe Prep2, a new murine homeobox-containing gene closely related to Prep1. The PREP2 protein belongs to the three amino acid loop extension (TALE) superclass of homeodomain-containing proteins and encodes a polypeptide of 462 residues. As for PREP1, PREP2 binds an appropriate site on DNA as a heterodimer with PBX1A. Northern analysis, immunoblotting, immunohistochemistry, and in situ hybridization show widespread Prep2 expression during organogenesis and in the adult. The data suggest that Prep2 functions to varying degrees in a broad array of tissues and developmental processes.

Animals↗
PubMed1995

Think before you prep: defining the terms of change in American healthcare.

United States physicians are grappling with a fundamental reorganization of the healthcare system. Although many remain skeptical of governmental efforts at reform, they seem to take as given an industrial efficiency model of change, including large and integrated managed care arrangements and performance measurement based on outcomes such as quality. This uncharacteristic acquiescence seems to derive in part from a confounding of concepts. This article makes three distinctions: among knowledge about practice, knowledge about quality, and outcomes research; between outcomes research and the outcomes movement; and between the outcomes movement and other options for healthcare reform. The suggestion that statistical measurement of specific variables ought not to have a priori precedence over other ways of thinking--and doing something--about healthcare is made.

Efficiency, Organizational↗
PubMed1993

Rapid isolation of DNA from trypanosomatid protozoa using a simple 'mini-prep' procedure.

Although several methods for isolating genomic DNA from trypanosomatid protozoa exist, all are time-consuming and cumbersome. Faster, simpler and efficient protocols for preparation of DNA from these protozoa are needed to ease the screening of mutants and transfectants. We describe the use of a bacterial lysis method to isolate chromosomal DNA from a wide range of trypanosomatids. The method is based on the finding reported by He et al., who noticed that phenol/chloroform treatment of Escherichia coli cells in the presence of LiCl and Triton X-100 solubilizes plasmid DNA, while precipitating unwanted chromosomal DNA and denatured cellular proteins. In applying this lysis method to the isolation of episomal DNA from transfected trypanosomatids, we found that, unlike bacterial genomic DNA, chromosomal DNA of trypanosomatids was soluble in the phenol/chloroform/Triton/LiCl mixture. This observation prompted us to use the bacterial lysis method as a routine protocol for extraction of DNA from trypanosomatids.

Animals↗