PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “SARCOMA/experimental”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 91 records · Page 5Linked to original sources
PubMed1962

The in vivo reutilization of lymphocytic and sarcoma DNA by cells growing in the peritoneal cavity.

An ascites tumor, Sarcoma I, was transplanted to isologous and homologous mice which had been labeled with tritiated thymidine from 1 to 24 hours previously. Radioautographic preparations revealed labeled host lymphocytes emerging to mingle with the transplanted tumor and the subsequent appearance of nuclear radioactivity in the sarcoma. Sarcoma cells cultured subcutaneously or in Millipore diffusion chambers in previously labeled mice did not demonstrate significant radioactivity. Transplantation of washed, H(3)-thymidine-labeled lymphocytes to non-radioactive, sarcoma-bearing mice was followed by the gradual appearance of nuclear radioactivity in the sarcoma. The label in the sarcoma was entirely removed by deoxyribonuclease but not by ribonuclease treatment prior to radioautography. Intraperitoneal injections of purified, H(3)-thymidine-labeled sarcoma or lymphoid DNA in normal or tumor-bearing mice were followed by radioactivity appearing in sarcoma or normal peritoneal mononuclear cells. It was concluded that reutilization of DNA and its metabolites may occur in vivo, and the conditions under which reutilization may be detected are discussed.

Animals↗
PubMed1957

The fine structure of the cells in mouse sarcoma 37 ascitic fluids.

The tumour cells and the reaction cells in Sarcoma 37 ascitic fluids have been studied in thin sections with the electron microscope. The reaction cells were either leucocytes or much larger acidophilic peritoneal cells of the same dimensions as the tumour cells; the peritoneal cells formed as much as 20 per cent of the large cell population. The fine structure of the cells is described and some new observations recorded. It has been found that the cell membrane of eosinophil granulocytes has a laminated composition and the characteristic granules of these cells a double limiting membrane. The pores in the double nuclear membrane of the peritoneal cells have been observed to have a fine line running across them. In the tumour cells, a rounded granular body with a central dense area has been found in the region of the centrosome; these cells were also seen to contain rows of parallel smooth surfaced cisternae lying 150 mmicro apart similar to those hitherto only observed in spermatids. There was a feltwork of fine filaments in the cytoplasm of the centrosome region of the tumour cells. The cytoplasmic fine structure underlying the basophilia of the tumour cells and the acidophilia of the peritoneal cells is compared and discussed.

Animals↗
PubMed1960

Cellular localization of Rous sarcoma virus as studied with fluorescent antibody.

A study has been made of the immunospecific, apple-green staining patterns imparted by fluorescent antibody to Rous virus in frozen sections of wing tumors produced in young chickens by various initiating doses of virus. Variations in the cellular site (cytoplasm, cell membrane, and nucleus), the distribution (particulate or homogeneous), and the quantity (little or much) of Rous viral antigens were seen. The per cent of sarcoma cells containing viral antigens was related to the tumor initiating dose of virus and to the infectivity titer of virus extracted from the wing tumor. With certain assumptions it was estimated that a quantity of virus approaching one tumor-producing dose (1 TD(50)) per tissue section was detectable with fluorescent antibody. Sarcoma cells containing viral antigens were identified as early as 4 days after inoculation of Rous virus. At this time viral antigens were also localized in the skeletal muscle of the inoculated wing in a characteristic focal pattern of deposition near or on the sarcolemma. This association between viral antigens and skeletal muscle fibers was observed also at 3 days after virus inoculation, when sarcoma cells were not yet demonstrable in the tissue sections. Viral antigens were detected in sarcoma cells in lung tumors of young chickens and in tumors of the chorioallantoic membrane of chick embryos.

Animals↗
PubMed1962

Sarcomas in hamsters after injection with Rous chicken tumor material.

Newborn hamsters were injected subcutaneously with a suspension of finely minced Rous chicken sarcoma (Schmidt-Ruppin strain). After an interval of about 2 weeks, progressively growing sarcomas developed at the site of injection in almost all animals. Also in adult hamsters inoculated intramuscularly with the same material sarcomas developed at the site of injection within 2 to 4 months. Secondary growths appeared on the peritoneal surface, in the retroperitoneal and mediastinal lymph nodes and in the lungs. The sarcomas usually had a pleomorphic appearance and showed a certain resemblance to rhabdomyosarcoma, but sometimes they had the character of spindle cell sarcomas of varying degree of maturity. Sarcomas were not only obtained in hamsters injected with cellular material from the Rous chicken sarcoma but were also seen in hamsters which were injected at birth or when 2 months' old with supernatant fluid obtained by repeated centrifugation of suspensions of homogenized chicken sarcoma, and presumed to be cell-free. The hamster sarcoma was transplanted to a newborn hamster and could then without difficulties be passed in series in hamsters. All attempts to transfer the sarcoma from hamster to hamster by means of cell-free material from the hamster sarcoma failed. On the other hand, material from the hamster sarcomas inoculated into chickens induced rapidly growing Rous sarcomas at the site of inoculation. This proved possible not only with material from the first but also from later passages of the tumor in hamsters. It is concluded that the strain of Rous virus used has the capacity to induce sarcomas not only in chickens but also in hamsters.

Animals↗
PubMed1954

Argon, xenon, hydrogen, and the oxygen consumption and glycolysis of mouse tissue slices.

The effects of xenon, argon, and hydrogen on the aerobic and anaerobic metabolism of mouse liver, brain, and sarcoma slices have been investigated. Xenon was found to alter the rates of metabolism of these tissues in a manner almost identical with helium. The gas increased the rate of oxygen consumption in all three tissues and significantly depressed that of anaerobic glycolysis in brain and liver. The depression of glycolysis in sarcoma was less pronounced and not highly significant. Although both the magnitude and statistical significance of the effects observed with argon were much smaller, there was a seeming adherence to the general pattern established by xenon and helium. Hydrogen while remaining essentially ineffective insofar as oxygen uptake was concerned, depressed glycolysis in both liver and brain slices but did not significantly affect sarcoma slices. The following points are stressed in the Discussion: (1) the magnitude and direction of effects exerted by helium, argon, xenon, hydrogen, and nitrogen do not conform with the relative values of molecular weight, density, and solubility of these gases; (2) the effect of these gases on tissue metabolism does not necessarily parallel that exerted upon the whole organism.

Animals↗