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[Frequency of sex chromatin among mental patients].

The frequency of sex chromatin of 2000 cases of mental patients are observed in this paper. The frequency of Y chromatin in male and X chromatin in female was 28.01% and 26.86% respectively, whereas only 16.97% and 17.84% respectively in the control group. The mental patient group is higher than the control group significantly (P < 0.0001). It may be influenced by some psychotropic drugs and the factor of course. Positive X chromatin in the male patients was 8 cases and karyotypes of chromosome was XXY type all. The incidence of XXY type is 0.77% and significant higher than the control group (0.18%, P < 0.01). It suggested that ratio of add one X chromosome in the male mental patients be obviously increased. The authors indicated if this examination be routinely used in clinic that would prevent misdiagnosis and would raise accuracy of diagnosis.

Adolescent↗

Sex chromatin in mammalian bone.

The presence of a sex chromatin body similar to that reported in other tissues has been demonstrated in the nuclei of osteoblasts, osteocytes, and periosteal cells from female dogs and cats.

Animals↗

[Movement of corneal endothelium after penetrating keratoplasty. Observation of sex chromatin as a cell marker].

There is a hypothesis that corneal endothelial cells migrate after penetrating keratoplasty from the areas of higher cell density to the lower cell density areas. In order to confirm this hypothesis, the author performed exchange penetrating keratoplasty in normal albino rabbits using four combinations of sex and age and examined the movement of the endothelium between donors and recipients by observing the density and sex chromatin of the endothelial cells. As a result, there was no significant difference of the rate of decrease of the endothelial cell density of the postoperative grafts between young and old grafts. In the experiment using the sex chromatin of the endothelial cells as a cell marker, it was concluded that the endothelium of the rabbit cornea migrate from the young grafts to the old recipients or from the young recipients to the old grafts. In the control study exchanging rabbit grafts between animals of different sex but the same age the count of the sex chromatin of the endothelium of the grafts and recipients did not change postoperatively. The results confirmed the above hypothesis concerning the migration of the transplanted corneal endothelial cells in the rabbit cornea.

Age Factors↗

Incidence of sex chromatin in Gallus domesticus.

The presence of so-called sex chromatin has been demonstrated in the interphase nuclei of the cells of the domestic chicken (Gallus domesticus). Definite sex dimorphism was observed for the incidence of this nuclear component; the frequency of its occurrence in females was at least ten times that of its occurrence in the males, ranging from 22 percent in the duodenal muscle cells to 52 percent in the epidermal cells of a growing feather.

Animals↗

Frequency of sex chromatin in the buccal smear of newborn females and their mothers during first six days after delivery.

The frequencies of sex chromatin in the buccal smear of the newborn females and their mothers were low on the first post-partum day and it increased gradually during the second and third day. By the fourth and fifth day it stabilized and the incidence of sex chromatin both in the mothers and the children became similar, although the frequency on the first day was significantly lower in the newborn. The incidences of pyknotic cells in the buccal smears of the newborns and their mothers were highest on day one and these declined rapidly in the following days. The significance of these findings have been discussed.

Adolescent↗

Sex chromatin and chromosome numbers in Lepidoptera.

Three classes of sex chromatin (SC) distribution have been encountered in a sample of Canadian Lepidoptera. In 78 of the 103 species examined, females were SC positive and males were SC negative. In another 24 species, females and males were SC negative. Females and males of a single species, Eucordylea resinosae, had two equal-sized SC bodies in interphase nuclei. In no species were females found to be SC negative and males SC positive. Usually presence of SC indicates an XX male and XY female sex determining mechanism, and its absence, XX male; XO female, but the exceptional occurrence of SC in females and males of a species suggests the need for caution in too universal an application of this interpretation. Chromosome numbers have also been determined for 53 of the 103 species. Male haploid numbers ranged from 11 to 61, but 30 was most frequently encountered.

Animals↗

Sex chromatin analysis of lymphocytes invading host organs in transfusion-associated graft-versus-host disease.

We report a method of sex chromatin analysis of lymphocytes separated from host organs in transfusion-associated graft-versus-host disease (GVHD), which enabled the demonstration of invasion by donor lymphocytes. The lymphocytes examined were separated from deparaffinized tissue blocks of skin, spleen and bone marrow from two female patients with transfusion-associated GVHD by incubation in 0.5% pepsin. The tissues had been removed at autopsy and fixed in formalin. Sex chromatin analysis was performed by fluorescence microscopy on separated lymphocytes stained with 0.005% quinacrine dihydrochloride. By this means Y chromatin-positive (i.e. male) lymphocytes were demonstrated in the skin, spleen and bone marrow of both female patients.

Aged↗

Studies on the chromosomes and sex chromatin in the horse.

This study provides accumulated data to assist the definition of karyotypes from normal and infertile horses. The normal karyotype of the horse (2n = 64) was characterized following Giemsa staining and C- banding, and 23% aneuploidy was found among chromosome counts of cells prepared from 44 clinically normal horses and 24 equine embryos. These expected variations in chromosome counts are especially important in the evaluation of potential mosaicism. Centromere staining was shown to be a valuable aid for the identification of specific chromosomes, in particular the sex chromosomes. Sex chromatin studies were applied to nerve tissue and polymorphonuclear neutrophils obtained from three horses. Distinctive sex chromatin bodies were detected in 70% of neurones from a normal mare. The sex chromatin was most frequently located adjacent to the nucleolus. Nuclear appendages ("drumsticks") were present in 4% of polymorphonuclear neutrophils from a normal mare. Small numbers of similar structures were noted in the neutrophils from each animal examined.

Journal Article↗