Effect of somatotropin on cells in tissue culture.
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Growth hormone isolated from human pituitaries has been demonstrated to be a good antigen in the rabbit. With the rabbit antiserum to human somatotropin, it is possible to detect as little as 0.1 microg of the hormone by precipitin test. The antiserum was also capable of neutralizing the growth-promoting activity of human somatotropin.
Female rats at the body weight plateau which responded to 13 daily injections of 0.4 mg of growth hormone by greater weight gain per gram of food had a decreased concentration of lipid aldehydes in blood plasma, an increased concentration in liver, and no change in intestine, compared with control rats given isotonic saline.
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