PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “Tetranitromethane”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 91 records · Page 5Linked to original sources
PubMed1984

Selective nitration of tyrosines-26 and -64 in bacteriorhodopsin with tetranitromethane.

Nitration of tyrosine-26 at pH 9.0 in bacteriorhodopsin does not change its absorption spectrum but lowers the apparent pK of the alkaline transition to a blue-shifted chromophore from about pH 12.0 to 10.6. This effect is reversed by reducing the nitrotyrosine-26 to aminotyrosine which demonstrates that the protonation state of tyrosine-26 and the alkaline chromophore transition are correlated. Nitration of tyrosine-64 resulted in a shift of the purple complex from 570 to 535 nm at neutral pH. The alkaline transition pK of such a nitrated membrane was below 10 but was clearly independent of the protonation state of tyrosine-64 because it is not reversed by reduction of the nitrotyrosine. Nitrotyrosine-26 showed spectral properties similar to L-nitrotyrosine in aqueous environment while nitrotyrosine-64 showed only a 360-nm absorbance in the apomembrane but not in the retinal-containing membrane. Both tyrosines are accessible to water-soluble reagents.

Bacteriorhodopsins↗