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PubMed2002

[Classification for one pathogenic Vibrio anguillarum strain isolated from skin-ulcer flounder].

A pathogenic bacterial strain M3 revealed gram negative rod shape, motile and translucent clone was studied. This bacterium was isolated from the skin-ulcer flounder in the fish farm of RongCheng City, Shandong Provice, and could not be identified by BIOLOG ID SYSTEM. Comparative 16S rDNA sequence analyses revealed that strain M3 was most related to the genus of Vibrio. The overall similarity value between strain M3 and Vibrio species were 94% to 98%. Phylogentic analysis showed that M3 strain exhibited the highest level of similarity to Vibrio anguillarum, and the biological features of strain M3 were very similar to that of Vibrio anguillarum. Based on the results obtained, strain M3 was identified as Vibrio anguillarum.

Animals↗
PubMed1982

Current approaches to the classification, characterisation and typing of pathogenic anaerobic bacteria.

Characterisation systems based on the demonstration of soluble antigens and toxins are of much practical value but have clear taxonomic limitations. This is illustrated by reference to our work with some of the pathogenic clostridia. The definition and classification of the anaerobic cocci is a difficult and continuing challenge. Systems for the classification and characterisation of the gram-negative non-sporing anaerobic bacilli are evolving well, though there are still some very difficult areas. A selection of the most helpful tests is indicated. Biotyping systems are compared with serotyping systems; the results of serological approaches to characterisation are encouraging. The further exploration of bacteriocin typing seems to be merited. The challenge of characterisation and classification of anaerobic spirochaetes commonly involved in human infections must now be taken up.

Amino Acids↗
PubMed1993

Emerging fungal pathogens in immunocompromised patients: classification, diagnosis, and management.

Fungi such as Fusarium species, Trichosporon species, Curvularia species, and Alternaria species previously were thought to represent contamination or harmless colonization when isolated from immunocompromised patients. More recently, the pathogenic role of these and other fungi has been clearly established. Three diverse groups of fungi are responsible for these emerging infections: the agents of phaeohyphomycosis and hyalohyphomycosis and certain yeasts. Reports of the emergence of these organisms as significant pathogens may be ascribed to increasing awareness by physicians and microbiologists, aggressive culture of patient specimens, increasingly cytotoxic chemotherapy, and selection of resistant organisms by the widespread empirical use of amphotericin B. Infections with these fungi tend to be disseminated and are frequently fatal in immunocompromised hosts. Treatment of these infections is not standardized. Experimental therapy in murine models of fungal infections suggests a role for newer agents, combination antifungal chemotherapy, and immunotherapy.

Fungi↗
PubMed1975

[Evaluation of the lysozyme and DNAase activity for the identification of pathogenic staphylococci].

The sensitivity of two tests recently proposed for the classification of pathogen staphylococci were evaluated: --production of DNA-ase with the modified method of Lachica et al.; --production of lisozyme. The two above tests were studied with other six tests on 1,000 strains of staphylococci showing a very high specificity. The Authors propose that the DNA-ase production and the lisozyme production, also for their very simple execution, should become routine tests to identify the strains of pathogenic staphylococci.

Deoxyribonucleases↗
PubMed1994

Utility of newer techniques for classification and identification of pathogenic anaerobic bacteria.

Results of genetic and biochemical analyses have broadened our understanding of taxonomic relationships among groups of anaerobic bacteria and have led to a better understanding of the pathogenesis of infection. Conventional bacteriologic methods are still of prime importance for the detection and identification of anaerobic pathogens. The use of nucleic acid probes has so far been restricted to research laboratories. A polymerase chain reaction-generated probe would be most useful for the rapid detection of toxigenic Clostridium difficile in feces. Probes are needed for detection of periodontopathogenic bacteria in dental plaque. Use of nucleic acid probes may become a useful adjunct to classic methodology in reference and teaching laboratories.

Bacteria, Anaerobic↗