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At least 91 records · Page 5Linked to original sources
PubMed1994

Increased yields of pathogenic Yersinia enterocolitica strains by cold enrichment.

Yersinia strains recovered from routine stool cultures (n = 13,534) as well as clinical symptoms in the patients were analysed in order to establish whether enteric Yersinia enterocolitica strains recovered only after cold enrichment cultures shared the same pathogenicity markers and caused the same symptoms as strains from primary cultures. 93% of the 201 Yersinia isolates were Y. enterocolitica strains and 71% of these were of serotype O3. Nearly 40% of all Y. enterocolitica strains and 21% of serotype O3 strains were isolated only after 1 week's cold enrichment of stool specimens. Practically all Y. enterocolitica O3 strains, whether from primary or cold enrichment cultures, were pathogenic not only on the basis of the serotype but also on the basis of Congo-red uptake and calcium-dependent growth at 35 degrees C (CR-MOX test). The symptoms in patients with Y. enterocolitica O3 from primary and cold enrichment cultures were similar except that abdominal pains were more frequent (p < 0.05) in the former. Arthropathia, mesenteric lymphadenitis and erythema nodosum were detected in 15% of the patients with Yersinia isolates and were more frequent in patients with isolates from cold enrichment or without diarrhea.

Abdominal Pain↗
PubMed2000

High relative humidity increases yield, harvest index, flowering, and gynophore growth of hydroponically grown peanut plants.

Growth chamber experiments were conducted to study the physiological and growth response of peanut (Arachis hypogaea L.) to 50% and 85% relative humidity (RH). The objective was to determine the effects of RH on pod and seed yield, harvest index, and flowering of peanut grown by the nutrient film technique (NFT). 'Georgia Red' peanut plants (14 days old) were planted into growth channels (0.15 x 0.15 x 1.2 m). Plants were spaced 25 cm apart with 15 cm between channels. A modified half-Hoagland solution with an additional 2 mM Ca was used. Solution pH was maintained between 6.4 and 6.7, and electrical conductivity (EC) ranged between 1100 and 1200 microS cm-1. Temperature regimes of 28/22 degrees C were maintained during the light/dark periods (12 hours each) with photosynthetic photon flux (PPF) at canopy level of 500 micromoles-m-2s-1. Foliage and pod fresh and dry weights, total seed yield, harvest index (HI), and seed maturity were greater at high than at low RH. Plants grown at 85% RH had greater total and individual leaflet area and stomatal conductance, flowered 3 days earlier and had a greater number of flowers reaching anthesis. Gynophores grew more rapidly at 85% than at 50% RH.

Arachis↗
PubMed1985

Increased yield of human tissue-type plasminogen activator obtained by means of recombinant DNA technology.

Extra copies of the human tissue-type plasminogen activator (t-PA) gene were introduced into the Bowes melanoma cell line. We obtained a recombinant cell line (TRBM6) which secretes approximately ten-fold more t-PA than the parent cell line. The identity of the plasminogen activator made by the new cell line was confirmed by sizing on sodium dodecyl sulphate polyacrylamide gels and by specific quenching using anti-t-PA antibody. We estimate that the recombinant line produces t-PA at a rate of approximately 3 pg/cell/24 hr and that t-PA accumulates in the harvest medium at a rate of approximately 4000 International t-PA Units/ml/24 hr.

Cell Line↗
PubMed1997

Increased yield of F VIII from pooled plasma vs. single-donor plasma in the production of cryoprecipitates.

Coagulation factor VIII:C yield was studied in two types of cryoprecipitates. The first group contained products from single-donor plasma units. The other group contained cryoprecipitates which were produced from pooled plasma. The volume of plasma/bag was not different between the two groups, but both the yield and the total content of F VIII:C in cryoprecipitates were significantly different. The yield of F VIII:C was higher (+20% in relative terms) in cryoprecipitates produced from pooled plasma, resulting in higher potency of such products. The positive effect of plasma pooling on the recovery of F VIII:C might be a result of reassembly of factor VIII subunits of different individuals in the plasma pools. The findings may have a role also in large-scale production of F VIII concentrates.

Chemical Precipitation↗
PubMed1991

CO(2)-Enhanced Yield and Foliar Deformation among Tomato Genotypes in Elevated CO(2) Environments.

Yield increases observed among eight genotypes of tomato (Lycopersicon esculentum Mill.) grown at ambient CO(2) (about 350) or 1000 microliters per liter CO(2) were not due to carbon exchange rate increases. Yield varied among genotypes while carbon exchange rate did not. Yield increases were due to a change in partitioning from root to fruit. Tomatoes grown with CO(2) enrichment exhibited nonepinastic foliar deformation similar to nutrient deficiency symptoms. Foliar deformation varied among genotypes, increased throughout the season, and became most severe at elevated CO(2). Foliar deformation was positively related to fruit yield. Foliage from the lower canopy was sampled throughout the growing season and analysed for starch, K, P, Ca, Mg, Fe, and Mn concentrations. Foliar K and Mn concentrations were the only elements correlated with deformation severity. Foliar K decreased while deformation increased. In another study, foliage of half the plants of one genotype received foliar applications of 7 millimolar KH(2)PO(4). Untreated foliage showed significantly greater deformation than treated foliage. Reduced foliar K concentration may cause CO(2)-enhanced foliar deformation. Reduced K may occur following decreased nutrient uptake resulting from reduced root mass due to the change in partitioning from root to fruit.

Journal Article↗
PubMed1978

Increased production of the antibiotic aurodox (X-5108) by aurodox-resistant mutants.

Conventional strain and media improvement techniques were of limited success in increasing yields of the antibiotic aurodox (X-5108) above 0.5 g/liter. Higher yields were obtained by reversion of a zero producer followed by the selection of mutants resistant to aurodox. Resistant strains of Streptomyces goldiniensis ATCC 21386 able to grow on 2 g/liter of aurodox produced greater than 2.5 g/liter of antibiotic. The rate of yield increase leveled off as the strains became resistant to greater than 2 g/liter of aurodox. These strains, in contrast to those sensitive to aurodox, gave a positive response to conventional mutagenic methods for further yield increases.

Aurodox↗
PubMed2004

Liquid culture mass production of biocontrol nematodes, Heterorhabditis bacteriophora (Nematoda: Rhabditida): improved timing of dauer juvenile inoculation.

Heterorhabditis bacteriophora is used in biological control of soil-borne insect pests in horticulture and turf. Mass production is carried out in monoxenic liquid cultures pre-incubated with the symbiont of the nematodes, the bacterium Photorhabdus luminescens, before nematode dauer juveniles (DJ) are inoculated. As a response to bacterial food signals, the DJ recover from the developmentally arrested dauer stage, grow to adults and produce DJ offspring. Variable DJ recovery after inoculation into cultures of P. luminescens often causes process failure due to low numbers of adult nematodes in the medium. In order to enhance DJ recovery, improve nematode population management and increase yields, the optimal timing for DJ inoculation was sought. The process parameter pH and respiration quotient (RQ) were recorded in order to test whether changes can be used to identify the best moment for DJ inoculation. When DJ were inoculated during the lag and early logarithmic growth phases of P. luminescens cultures, DJ recovery was low and almost no nematode reproduction was obtained. High populations of P. luminescens phase variants were recorded. Recovery and yields increased when DJ were inoculated during the latter log phase during which the RQ dropped to values <0.8 and the pH reached a maximum. The highest DJ recovery and yields were observed in cultures that were inoculated during the late stationary growth phase. This period started with the increase of the pH after its distinct minimum at pH <8.0. Thus optimal timing for DJ inoculation can be defined through monitoring of the pH in the P. luminescens culture.

Animals↗
PubMedDate not supplied

Multicenter comparison of the high volume (10 ml) NR BACTEC PLUS system and the standard (5 ml) NR BACTEC system.

This multicenter study was designed to compare the new BACTEC PLUS system (nonradiometric), which utilizes an 8- to 10-ml blood inoculum in a resin-containing medium, to the standard BACTEC (nonradiometric) without resins and 5-ml blood inoculum. There were 12,341 compliant sets studied, yielding 1331 positives, with 1099 sets deemed clinically significant. Overall the BACTEC PLUS showed an enhanced recovery of 33% (p less than 0.001) over its standard counterpart, with significant yield increased in the staphylococci (p less than 0.001), streptococci (p less than 0.002), pseudomonads (p less than 0.002), Enterobacteriaceae (p less than 0.001), and other aerobic Gram negatives (p less than 0.02). The enhanced performance increased to 53% if the patient was receiving any antibiotics at the time the blood was cultured. In patients known to be free of antibiotics at the time of blood draw, there was still an increased yield of 18%. The new system detected positivity at least one reading sooner than twice as often as the converse, and confirmed septic episodes significantly more often (21% overall) (41% on antibiotics) (15% no antibiotics). The BACTEC PLUS has distinct advantages over its low blood volume, nonresin counterpart.

Bacteria↗